BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov11c06 (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.007 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.047 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.11 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.11 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.14 At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.0 At4g14830.1 68417.m02280 expressed protein 29 2.4 At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 4.1 At1g22080.1 68414.m02761 hypothetical protein 28 4.1 At3g49540.1 68416.m05414 expressed protein 28 5.4 At1g76770.1 68414.m08934 heat shock protein-related contains sim... 28 5.4 At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 28 5.4 At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 27 7.2 At2g28680.1 68415.m03486 cupin family protein similar to legumin... 27 7.2 At3g43230.1 68416.m04563 zinc finger (FYVE type) family protein ... 27 9.5 At3g16857.2 68416.m02153 two-component responsive regulator fami... 27 9.5 At3g16857.1 68416.m02152 two-component responsive regulator fami... 27 9.5 >At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (HSP22.0-ER) identical to endomembrane-localized small heat shock protein GI:511795 from [Arabidopsis thaliana] Length = 195 Score = 37.5 bits (83), Expect = 0.007 Identities = 20/48 (41%), Positives = 31/48 (64%) Frame = +3 Query: 426 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 +F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 34.7 bits (76), Expect = 0.047 Identities = 18/50 (36%), Positives = 31/50 (62%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+ Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 33.5 bits (73), Expect = 0.11 Identities = 18/50 (36%), Positives = 30/50 (60%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+ Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 33.5 bits (73), Expect = 0.11 Identities = 19/50 (38%), Positives = 32/50 (64%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+ Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 33.1 bits (72), Expect = 0.14 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 524 S QF RR+ LPE + V++ + +GVLT+T P+ Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138 >At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock protein (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family: identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 134 Score = 30.3 bits (65), Expect = 1.0 Identities = 18/79 (22%), Positives = 33/79 (41%) Frame = +3 Query: 294 DKFQVNLDVQHFAPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 473 D ++D+ EEI V+ D ++ + F R++ LPE Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94 Query: 474 VESRLSSDGVLTITAPRKV 530 + + DGVLT+ P+++ Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 29.1 bits (62), Expect = 2.4 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +3 Query: 432 VRRYALPEGAAPETVESRLSSDGVLTITAPR 524 V R+ LPE PE V DG L +T P+ Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139 >At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyltransferase family protein similar to defense-related protein cjs1 [Brassica carinata][GI:14009292][Mol Plant Pathol (2001) 2(3):159-169] Length = 352 Score = 28.3 bits (60), Expect = 4.1 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = +1 Query: 430 SSDVTRCLKARRLRLWNRDCHQTGYSPSLRR 522 +S + + + R LWNRD H TG++ +++ Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFNNKVKK 181 >At1g22080.1 68414.m02761 hypothetical protein Length = 475 Score = 28.3 bits (60), Expect = 4.1 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = -1 Query: 220 LFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKYGSND 92 L + P A L+R + PK RRR R+ SSK + + D Sbjct: 305 LMDEAPRANLKRKQEQQDEPKRSKKRRRQRVARSSSKLHSNLD 347 >At3g49540.1 68416.m05414 expressed protein Length = 166 Score = 27.9 bits (59), Expect = 5.4 Identities = 17/47 (36%), Positives = 27/47 (57%) Frame = +3 Query: 453 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIAQTGPVRKE 593 E AA E E+++ + + + AP KV AV+ E+K A+ PV+ E Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAE 140 >At1g76770.1 68414.m08934 heat shock protein-related contains similarity to 17.9 kDa heat-shock protein [Helianthus annuus] gi|11990130|emb|CAB55634 Length = 244 Score = 27.9 bits (59), Expect = 5.4 Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%) Frame = +3 Query: 423 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKV 530 ++F + + +P+ + +++R + DG LT+T P+KV Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129 >At1g66880.1 68414.m07601 serine/threonine protein kinase family protein contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 1296 Score = 27.9 bits (59), Expect = 5.4 Identities = 13/41 (31%), Positives = 18/41 (43%) Frame = -1 Query: 226 YSLFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKY 104 Y S G A + +ARP CWS R +E S+ + Sbjct: 1041 YEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSF 1081 >At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, putative similar to trehalose-6-phosphate phosphatase (AtTPPA) GI:2944178; contains Pfam profile PF02358: Trehalose-phosphatase Length = 377 Score = 27.5 bits (58), Expect = 7.2 Identities = 16/57 (28%), Positives = 28/57 (49%) Frame = +3 Query: 126 SRRPRRLLDQHFGLALTPDDLLSVAAGPLLNREYYRPWRHLAAAARDVGSSIKVDKD 296 S PR+ L + F + + P+D S A L + H+AA A++ ++ +D D Sbjct: 59 SSPPRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115 >At2g28680.1 68415.m03486 cupin family protein similar to legumin (11S-globulin) from Ginkgo biloba [GI:949869], 11S globulin from Avena sativa [GI:472867]; contains a 11-S plant seed storage protein signature (PS00305) Length = 356 Score = 27.5 bits (58), Expect = 7.2 Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 1/47 (2%) Frame = +3 Query: 420 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK 557 S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRK 186 >At3g43230.1 68416.m04563 zinc finger (FYVE type) family protein contains Pfam domain PF01363: FYVE zinc finger Length = 485 Score = 27.1 bits (57), Expect = 9.5 Identities = 11/15 (73%), Positives = 12/15 (80%) Frame = +1 Query: 520 RGRCLTPSRERERCP 564 +GRCL PSR RER P Sbjct: 211 KGRCLMPSRFRERNP 225 >At3g16857.2 68416.m02153 two-component responsive regulator family protein / response regulator family protein contains Pfam profile: PF00072 response regulator receiver domain; similar to ARR1 protein GB:BAA74528 from [Arabidopsis thaliana] (Plant Cell Physiol. (1998) 39 (11), 1232-1239) Length = 690 Score = 27.1 bits (57), Expect = 9.5 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +1 Query: 376 WKASTKRRKTSTGIFQGSSSDVTRC-LKARRLRLWNRDCHQ 495 W++S+++RK G QG D LK R+ +W+ + HQ Sbjct: 209 WRSSSRKRKDEEGEEQGDDKDEDASNLKKPRV-VWSVELHQ 248 >At3g16857.1 68416.m02152 two-component responsive regulator family protein / response regulator family protein contains Pfam profile: PF00072 response regulator receiver domain; similar to ARR1 protein GB:BAA74528 from [Arabidopsis thaliana] (Plant Cell Physiol. (1998) 39 (11), 1232-1239) Length = 669 Score = 27.1 bits (57), Expect = 9.5 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +1 Query: 376 WKASTKRRKTSTGIFQGSSSDVTRC-LKARRLRLWNRDCHQ 495 W++S+++RK G QG D LK R+ +W+ + HQ Sbjct: 209 WRSSSRKRKDEEGEEQGDDKDEDASNLKKPRV-VWSVELHQ 248 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,409,879 Number of Sequences: 28952 Number of extensions: 236721 Number of successful extensions: 631 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 618 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 631 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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