BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov11a04 (722 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.009 At3g49540.1 68416.m05414 expressed protein 36 0.027 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.063 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.14 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.14 At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing ... 33 0.19 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.19 At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.4 At4g14830.1 68417.m02280 expressed protein 29 3.1 At1g76770.1 68414.m08934 heat shock protein-related contains sim... 29 3.1 At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 4.1 At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 5.5 At1g22080.1 68414.m02761 hypothetical protein 28 5.5 At1g01340.1 68414.m00049 cyclic nucleotide-regulated ion channel... 28 5.5 At4g15410.1 68417.m02355 UBX domain-containing protein low simil... 28 7.2 At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 28 7.2 At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 27 9.5 At4g01010.1 68417.m00136 cyclic nucleotide-regulated ion channel... 27 9.5 At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2... 27 9.5 At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila... 27 9.5 At1g05830.1 68414.m00610 trithorax protein, putative / PHD finge... 27 9.5 >At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (HSP22.0-ER) identical to endomembrane-localized small heat shock protein GI:511795 from [Arabidopsis thaliana] Length = 195 Score = 37.5 bits (83), Expect = 0.009 Identities = 20/48 (41%), Positives = 31/48 (64%) Frame = +3 Query: 426 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 +F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178 >At3g49540.1 68416.m05414 expressed protein Length = 166 Score = 35.9 bits (79), Expect = 0.027 Identities = 22/64 (34%), Positives = 37/64 (57%), Gaps = 4/64 (6%) Frame = +3 Query: 453 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKEIKDQSEEAN 620 E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E + EE+ Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156 Query: 621 EKEK 632 ++EK Sbjct: 157 QEEK 160 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 34.7 bits (76), Expect = 0.063 Identities = 18/50 (36%), Positives = 31/50 (62%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+ Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 33.5 bits (73), Expect = 0.14 Identities = 18/50 (36%), Positives = 30/50 (60%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+ Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 33.5 bits (73), Expect = 0.14 Identities = 19/50 (38%), Positives = 32/50 (64%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 569 S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+ Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155 >At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing protein Length = 987 Score = 33.1 bits (72), Expect = 0.19 Identities = 25/92 (27%), Positives = 42/92 (45%), Gaps = 6/92 (6%) Frame = +1 Query: 400 KTSTGIFQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLRRGRCLTPS------RERERC 561 K++T + +++++R L+ + ++ Q SPS R + S R R R Sbjct: 474 KSATELAAARAAEISRKLRPDGVGNDEKEADQKSRSPSKSPARSRSKSKSPISYRRRRRS 533 Query: 562 PSHRPVPFARRSRIRVKKPTRRKSRKVYEELR 657 P++ P PF R R + P R + R YE R Sbjct: 534 PTYSP-PFRRPRSHRSRSPLRYQRRSTYEGRR 564 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 33.1 bits (72), Expect = 0.19 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +3 Query: 420 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 524 S QF RR+ LPE + V++ + +GVLT+T P+ Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138 >At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock protein (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family: identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 134 Score = 30.3 bits (65), Expect = 1.4 Identities = 18/79 (22%), Positives = 33/79 (41%) Frame = +3 Query: 294 DKFQVNLDVQHFAPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 473 D ++D+ EEI V+ D ++ + F R++ LPE Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94 Query: 474 VESRLSSDGVLTITAPRKV 530 + + DGVLT+ P+++ Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 29.1 bits (62), Expect = 3.1 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +3 Query: 432 VRRYALPEGAAPETVESRLSSDGVLTITAPR 524 V R+ LPE PE V DG L +T P+ Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139 >At1g76770.1 68414.m08934 heat shock protein-related contains similarity to 17.9 kDa heat-shock protein [Helianthus annuus] gi|11990130|emb|CAB55634 Length = 244 Score = 29.1 bits (62), Expect = 3.1 Identities = 19/73 (26%), Positives = 40/73 (54%), Gaps = 3/73 (4%) Frame = +3 Query: 423 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKVPDAVKGERKVPIAQTGPVRKE-- 593 ++F + + +P+ + +++R + DG LT+T P+KV + G K+ KE Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV-KGITG-LKIEEEDEEEEMKEPI 150 Query: 594 IKDQSEEANEKEK 632 +++++EE E E+ Sbjct: 151 VEEKTEEKTEPEE 163 >At2g28680.1 68415.m03486 cupin family protein similar to legumin (11S-globulin) from Ginkgo biloba [GI:949869], 11S globulin from Avena sativa [GI:472867]; contains a 11-S plant seed storage protein signature (PS00305) Length = 356 Score = 28.7 bits (61), Expect = 4.1 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%) Frame = +3 Query: 420 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 590 S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+ Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199 Query: 591 EIKD 602 +IKD Sbjct: 200 DIKD 203 >At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyltransferase family protein similar to defense-related protein cjs1 [Brassica carinata][GI:14009292][Mol Plant Pathol (2001) 2(3):159-169] Length = 352 Score = 28.3 bits (60), Expect = 5.5 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = +1 Query: 430 SSDVTRCLKARRLRLWNRDCHQTGYSPSLRR 522 +S + + + R LWNRD H TG++ +++ Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFNNKVKK 181 >At1g22080.1 68414.m02761 hypothetical protein Length = 475 Score = 28.3 bits (60), Expect = 5.5 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = -2 Query: 220 LFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKYGSND 92 L + P A L+R + PK RRR R+ SSK + + D Sbjct: 305 LMDEAPRANLKRKQEQQDEPKRSKKRRRQRVARSSSKLHSNLD 347 >At1g01340.1 68414.m00049 cyclic nucleotide-regulated ion channel (CNGC10) (ACBK1) almost identical to CaM-regulated potassium ion channel (ACBK1) GI:8515883 from [Arabidopsis thaliana]; contains Pfam domain, PF00520: Ion transport protein Length = 706 Score = 28.3 bits (60), Expect = 5.5 Identities = 18/55 (32%), Positives = 27/55 (49%) Frame = +1 Query: 505 SPSLRRGRCLTPSRERERCPSHRPVPFARRSRIRVKKPTRRKSRKVYEELRLLQN 669 S ++R R+ E+ SHR +P R RIR + R + + EE LL+N Sbjct: 391 STTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEEETLLRN 445 >At4g15410.1 68417.m02355 UBX domain-containing protein low similarity to XY40 protein [Rattus norvegicus] GI:2547025; contains Pfam profile PF00789: UBX domain Length = 421 Score = 27.9 bits (59), Expect = 7.2 Identities = 15/37 (40%), Positives = 18/37 (48%) Frame = +1 Query: 490 HQTGYSPSLRRGRCLTPSRERERCPSHRPVPFARRSR 600 H Y+PS +PSR R PS R P+ RSR Sbjct: 75 HSPDYTPSETSP---SPSRSRSASPSSRAAPYGLRSR 108 >At1g66880.1 68414.m07601 serine/threonine protein kinase family protein contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 1296 Score = 27.9 bits (59), Expect = 7.2 Identities = 13/41 (31%), Positives = 18/41 (43%) Frame = -2 Query: 226 YSLFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKY 104 Y S G A + +ARP CWS R +E S+ + Sbjct: 1041 YEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSF 1081 >At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, putative similar to trehalose-6-phosphate phosphatase (AtTPPA) GI:2944178; contains Pfam profile PF02358: Trehalose-phosphatase Length = 377 Score = 27.5 bits (58), Expect = 9.5 Identities = 16/57 (28%), Positives = 28/57 (49%) Frame = +3 Query: 126 SRRPRRLLDQHFGLALTPDDLLSVAAGPLLNREYYRPWRHLAAAARDVGSSIKVDKD 296 S PR+ L + F + + P+D S A L + H+AA A++ ++ +D D Sbjct: 59 SSPPRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115 >At4g01010.1 68417.m00136 cyclic nucleotide-regulated ion channel, putative (CNGC13) similar to CaM-regulated potassium ion channel (ACBK1) GI:8515883 from [Arabidopsis thaliana] Length = 696 Score = 27.5 bits (58), Expect = 9.5 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = +1 Query: 505 SPSLRRGRCLTPSRERERCPSHRPVPFARRSRIRVKKPTRRKSRKVYEELRLLQN 669 S ++R R+ E+ SHR +P R RIR + + + + EE LL+N Sbjct: 397 STTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRRYEQYKWQETRGVEEENLLRN 451 >At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2 / CNBT2 (CNGC19) identical to cyclic nucleotide-binding transporter 2 (CNBT2) GI:8131900 from [Arabidopsis thaliana]; member of the cyclic nucleotide-gated channel family (CNGC)- see PMID:11500563 Length = 743 Score = 27.5 bits (58), Expect = 9.5 Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 1/52 (1%) Frame = +1 Query: 517 RRGRCLTPSRERERCPSHRPVPFARRSRIR-VKKPTRRKSRKVYEELRLLQN 669 RR + R+ E+ SHR +P R R+R V++ T +R V EEL L +N Sbjct: 488 RRMEMMLRKRDVEQWMSHRRLPEDIRKRVREVERYTWAATRGVNEEL-LFEN 538 >At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar to RNA helicase GB:A57514 GI:897915 from [Rattus norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 989 Score = 27.5 bits (58), Expect = 9.5 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = +2 Query: 536 RRQGREKGAHRTDRSRSQGDQGSE*RSQREGKVERFMK 649 +R+G +KG R +R R +G R +RE + E+ K Sbjct: 67 KRRGEDKGRGRRERDRDRGKYLKRDRERREREKEKGRK 104 >At1g05830.1 68414.m00610 trithorax protein, putative / PHD finger family protein / SET domain-containing protein similar to trithorax-like protein 1 [Arabidopsis thaliana] GI:12659210; contains Pfam domain, PF00628: PHD-finger and PF00856: SET domain Length = 1056 Score = 27.5 bits (58), Expect = 9.5 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = +1 Query: 610 KKPTRRKSRKVYEELRLLQNGGATRD 687 +K RR+ R+ + EL +LQN G RD Sbjct: 89 RKRRRRRRRESFLELAILQNEGVERD 114 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,156,690 Number of Sequences: 28952 Number of extensions: 296004 Number of successful extensions: 846 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 823 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 846 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1575119672 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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