Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov10n17
(543 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family prot... 81 5e-16
At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa, pu... 64 8e-11
At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family prot... 63 1e-10
At4g33905.1 68417.m04811 peroxisomal membrane protein 22 kDa, pu... 59 2e-09
At1g52870.2 68414.m05978 peroxisomal membrane protein-related co... 50 1e-06
At1g52870.1 68414.m05977 peroxisomal membrane protein-related co... 45 4e-05
At2g42770.1 68415.m05296 peroxisomal membrane 22 kDa family prot... 37 0.008
At3g50160.1 68416.m05483 hypothetical protein contains Pfam prof... 32 0.22
At3g24570.1 68416.m03087 peroxisomal membrane 22 kDa family prot... 29 1.5
At1g79975.2 68414.m09353 expressed protein 28 4.6
At1g79975.1 68414.m09352 expressed protein 28 4.6
At3g50130.1 68416.m05480 expressed protein ; expression supporte... 27 8.1
At1g28430.1 68414.m03495 cytochrome P450, putative similar to cy... 27 8.1
>At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family protein
similar to SP|P42925 22 kDa peroxisomal membrane protein
{Mus musculus}; contains Pfam profile PF04117: Mpv17 /
PMP22 family
Length = 288
Score = 81.0 bits (191), Expect = 5e-16
Identities = 45/138 (32%), Positives = 71/138 (51%)
Frame = +1
Query: 130 YRFFKYYQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGFF 309
+ +YQ L P L +A+ L GD+I Q+ I KT D +RTL F +G
Sbjct: 112 WSLLSWYQALLSNSPVLTKAVTAALLNLVGDLICQLTINKT--SSLDKKRTLTFTFLGLG 169
Query: 310 IGGPALRTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDIIK 489
+ GP L WY L+K V + G + ++ LDQF+FAP F+ + L +V L+ K ++I
Sbjct: 170 LVGPTLHFWYLYLSKVVTASGLSGAVIRLLLDQFVFAPIFVGVFLSAVVTLEGKPSNVIP 229
Query: 490 VDLKSNYFDVLTTNYYIW 543
L+ + + N+ +W
Sbjct: 230 -KLQQEWTGAMIANWQLW 246
>At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa,
putative similar to 22 kDa peroxisomal membrane protein
{Mus musculus} SWISS-PROT:P42925, NCBI_gi:454833
Length = 252
Score = 63.7 bits (148), Expect = 8e-11
Identities = 35/136 (25%), Positives = 60/136 (44%)
Frame = +1
Query: 136 FFKYYQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGFFIG 315
F +Y ++ P + +++ + + D+ SQ I KT D+ RT + G G F+
Sbjct: 73 FVGWYLGMVKSHPVVTKSVTSSLIYIAADLSSQTIA-KTSSESYDLVRTARMGGYGLFVL 131
Query: 316 GPALRTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDIIKVD 495
GP L W+ +++ Q I T KK+ + Q ++ P I A+LQ + +I
Sbjct: 132 GPTLHYWFNFMSRLFPKQDLITTFKKMAMGQTIYGPIMTVIFFSLNASLQGERGSVILAR 191
Query: 496 LKSNYFDVLTTNYYIW 543
LK + L W
Sbjct: 192 LKRDLLPALFNGVMYW 207
>At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 254
Score = 63.3 bits (147), Expect = 1e-10
Identities = 38/137 (27%), Positives = 62/137 (45%), Gaps = 1/137 (0%)
Frame = +1
Query: 136 FFKYYQEFLRRRPYLVQAIQTGGLMATGDVISQVI-IEKTYWRDTDIQRTLKFGSIGFFI 312
F ++Y L P++ ++I T + D+ SQ+I +E T D+ RT + S G
Sbjct: 78 FLRWYLRKLESHPFMTKSITTSVIYMAADLTSQMITMEPT--GSFDLIRTARMASFGLIF 135
Query: 313 GGPALRTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDIIKV 492
GP+ W+ L+K + + + T KK+ + Q LF P + AALQ + + I
Sbjct: 136 LGPSQHLWFSYLSKILPKRDVLTTFKKIMMGQVLFGPVSNTVFYSYNAALQGENSEEIVA 195
Query: 493 DLKSNYFDVLTTNYYIW 543
LK + L W
Sbjct: 196 RLKRDLLPTLKNGLMYW 212
>At4g33905.1 68417.m04811 peroxisomal membrane protein 22 kDa,
putative similar to 22 kDa peroxisomal membrane protein
PMP22 [Mus musculus] gi|10954089|gb|AAG25724
Length = 261
Score = 59.3 bits (137), Expect = 2e-09
Identities = 34/139 (24%), Positives = 61/139 (43%)
Frame = +1
Query: 127 TYRFFKYYQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGF 306
T F +Y ++ RP L +++ + + D+ SQ I + + D+ RT + G G
Sbjct: 79 TAGFIGWYLGMVKSRPVLTKSVTSSLIYIAADLSSQTIPQASV-DSYDLVRTARMGGYGL 137
Query: 307 FIGGPALRTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDII 486
I GP L W+ +++ + I T KK+ + Q ++ P+ + AALQ + I
Sbjct: 138 LILGPTLHYWFNLMSSLFPKRDLITTFKKMAMGQTVYGPAMNVVFFSLNAALQGENGSEI 197
Query: 487 KVDLKSNYFDVLTTNYYIW 543
LK + + W
Sbjct: 198 VARLKRDLLPTMLNGVMYW 216
>At1g52870.2 68414.m05978 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 366
Score = 49.6 bits (113), Expect = 1e-06
Identities = 34/132 (25%), Positives = 64/132 (48%)
Frame = +1
Query: 148 YQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGFFIGGPAL 327
Y+E L++ P L + + +G + + GD I+Q K + + D RTL+ G +GF + G
Sbjct: 173 YEEALKQNPVLAKMVISGVVYSVGDWIAQCYEGKPLF-EIDRARTLRSGLVGFTLHGSLS 231
Query: 328 RTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDIIKVDLKSN 507
+Y + Q V KV DQ +++ + I + L+ ++ I +LK+
Sbjct: 232 HFYYQFCEELFPFQDWWVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPISIFKELKAT 291
Query: 508 YFDVLTTNYYIW 543
+ +LT + +W
Sbjct: 292 FLPMLTAGWKLW 303
>At1g52870.1 68414.m05977 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 306
Score = 44.8 bits (101), Expect = 4e-05
Identities = 33/130 (25%), Positives = 62/130 (47%)
Frame = +1
Query: 148 YQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGFFIGGPAL 327
Y+E L++ P L + + +G + + GD I+Q K + + D RTL+ G +GF + G
Sbjct: 173 YEEALKQNPVLAKMVISGVVYSVGDWIAQCYEGKPLF-EIDRARTLRSGLVGFTLHGSLS 231
Query: 328 RTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCILLISVAALQRKTWDIIKVDLKSN 507
+Y + Q V KV DQ +++ + I + L+ ++ I +LK+
Sbjct: 232 HFYYQFCEELFPFQDWWVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPISIFKELKAT 291
Query: 508 YFDVLTTNYY 537
+ +LT +
Sbjct: 292 FLPMLTVGSF 301
>At2g42770.1 68415.m05296 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 232
Score = 37.1 bits (82), Expect = 0.008
Identities = 29/107 (27%), Positives = 44/107 (41%), Gaps = 19/107 (17%)
Frame = +1
Query: 178 LVQAIQTGGLMATGDVISQV----------------IIEKTYWR---DTDIQRTLKFGSI 300
L QA+ G L TGD I+Q+ + E W + D R L+ S
Sbjct: 56 LKQAVTAGALTFTGDTIAQLSGRWKKRTALKQSSSELDEGELWNIFSEHDWIRALRMSSY 115
Query: 301 GFFIGGPALRTWYGVLNKYVGSQGKIVTIKKVFLDQFLFAPSFLCIL 441
GF + GP WY L+ + + KV L+Q + PS + ++
Sbjct: 116 GFLLYGPGSYAWYQFLDHSLPKPTATNLVLKVLLNQVILGPSVIAVI 162
>At3g50160.1 68416.m05483 hypothetical protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 511
Score = 32.3 bits (70), Expect = 0.22
Identities = 15/62 (24%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = +1
Query: 337 YGVLNKYVGSQGKIVTIKKVFLDQFLFAPS--FLCILLISVAALQRKTWDIIKVDLKSNY 510
YG++ ++GS ++ + + +F P+ +L L V R+ W+ +K L+ Y
Sbjct: 406 YGIIENWLGSDSEVSDLFNGLGKEVIFDPNDGYLSALTGEVNIYYRRKWNYLKATLRHKY 465
Query: 511 FD 516
F+
Sbjct: 466 FN 467
>At3g24570.1 68416.m03087 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 235
Score = 29.5 bits (63), Expect = 1.5
Identities = 39/171 (22%), Positives = 60/171 (35%), Gaps = 34/171 (19%)
Frame = +1
Query: 133 RFFKYYQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWR------------DTDIQ 276
+ +++YQ L P Q I +G L GDV +Q I T R D D +
Sbjct: 3 KLWRWYQRCLTVHPVKTQVISSGFLWGFGDVTAQYITHSTAKRRLLRLTETNKDADADAE 62
Query: 277 RTLKF----------------GSIGFFIGGPALRTWYGVLNKYVGSQGKIV------TIK 390
+K+ GF GP WY L+K++ + + V
Sbjct: 63 IKVKWKQDAEFKVNWKRVAITSMFGFGFVGPVGHFWYEGLDKFIKLKLRYVPKSTRFVAA 122
Query: 391 KVFLDQFLFAPSFLCILLISVAALQRKTWDIIKVDLKSNYFDVLTTNYYIW 543
KV +D +F P L + + K +K LK ++ L W
Sbjct: 123 KVAMDGLIFGPVDLLVFFTYMGFATGKNTAEVKEGLKRDFLPALALEGGAW 173
>At1g79975.2 68414.m09353 expressed protein
Length = 152
Score = 27.9 bits (59), Expect = 4.6
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2
Query: 530 LVVKTSK*LDFKSTLIISHVFLC 462
LV + K LDF +TL I H+F C
Sbjct: 82 LVERARKCLDFSATLYIIHLFFC 104
>At1g79975.1 68414.m09352 expressed protein
Length = 152
Score = 27.9 bits (59), Expect = 4.6
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2
Query: 530 LVVKTSK*LDFKSTLIISHVFLC 462
LV + K LDF +TL I H+F C
Sbjct: 82 LVERARKCLDFSATLYIIHLFFC 104
>At3g50130.1 68416.m05480 expressed protein ; expression supported
by MPSS
Length = 564
Score = 27.1 bits (57), Expect = 8.1
Identities = 14/61 (22%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +1
Query: 340 GVLNKYVGSQGKIVTIKKVFLDQFLFAP--SFLCILLISVAALQRKTWDIIKVDLKSNYF 513
G++ ++G+ ++ + + F P S+L L V + W+++K LK YF
Sbjct: 470 GIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYSRKWNVLKAILKHKYF 529
Query: 514 D 516
+
Sbjct: 530 N 530
>At1g28430.1 68414.m03495 cytochrome P450, putative similar to
cytochrome P450 (CYP93A1) GI:1435059 from [Glycine max]
Length = 521
Score = 27.1 bits (57), Expect = 8.1
Identities = 15/54 (27%), Positives = 21/54 (38%)
Frame = +1
Query: 148 YQEFLRRRPYLVQAIQTGGLMATGDVISQVIIEKTYWRDTDIQRTLKFGSIGFF 309
Y FL R + V + D+ I +Y D I + FGS G+F
Sbjct: 72 YGPFLHLRIFHVPIVLVSSASTAYDIFKTNDINVSYRGDVAIDECIVFGSFGYF 125
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,171,568
Number of Sequences: 28952
Number of extensions: 216063
Number of successful extensions: 444
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 422
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 441
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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