BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10m12 (613 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 209 2e-55 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 119 4e-28 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 116 2e-27 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 116 2e-27 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 113 2e-26 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 111 1e-25 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 92 5e-20 SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 29 0.53 SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosacch... 25 6.5 SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 8.6 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 209 bits (511), Expect = 2e-55 Identities = 96/167 (57%), Positives = 125/167 (74%) Frame = +3 Query: 105 GFDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVT 284 GFDR IT+FSPEGRLYQVEYA KA N G+TSV + G + A V +Q+KVPD+L+D +V Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVK 65 Query: 285 HLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQ 464 H+F +T IGCVMTG IAD+R+QV RAR EAA ++YK G +P +L +R+A+I+QV TQ Sbjct: 66 HVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQ 125 Query: 465 NAEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAGAKTVD 605 A MRPLG +M L+A DDE GP ++K DP+G+Y YKA +AG K + Sbjct: 126 RAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTE 172 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 119 bits (286), Expect = 4e-28 Identities = 62/168 (36%), Positives = 97/168 (57%), Gaps = 1/168 (0%) Frame = +3 Query: 99 SAGFDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLD-PV 275 S +D TIFSPEGRLYQVEYAL+AIN G+ ++ + D V+AA++KV +LL+ Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGV-ALGIVAKDGIVLAAEKKVTSKLLEQEE 60 Query: 276 SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQV 455 S L+ + D + C + G+ AD+ + AR + + E+P L RR+ D+ Q Sbjct: 61 SAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQG 120 Query: 456 YTQNAEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAGAKT 599 YTQ +RP G S + +D G +++++PSG Y S++A + G + Sbjct: 121 YTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNS 168 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 116 bits (280), Expect = 2e-27 Identities = 67/159 (42%), Positives = 94/159 (59%), Gaps = 2/159 (1%) Frame = +3 Query: 120 ITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFAL 299 +T FSP G+L Q+EYAL A+N G+TSV ++ TD V+A ++K L S+ + A+ Sbjct: 9 LTTFSPNGKLVQIEYALNAVN-AGVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAI 67 Query: 300 TDRIGCVMTGMIADSRSQVQRARYEAANWQYK--YGSEIPVHILCRRIADISQVYTQNAE 473 T IG V +GM D R V ++R + A YK Y P IL + IA + Q TQ+ Sbjct: 68 TPDIGMVYSGMGPDFRVLVDKSR-KVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGG 126 Query: 474 MRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAG 590 +RP G S+ L+A DE GP +Y+ DPSG Y ++KA A G Sbjct: 127 VRPFGVSL-LVAGMDEKGPSLYQVDPSGTYFAWKATAIG 164 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 116 bits (280), Expect = 2e-27 Identities = 63/166 (37%), Positives = 99/166 (59%), Gaps = 3/166 (1%) Frame = +3 Query: 102 AGFDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRK-VPDRLLDPVS 278 +G+DR +++FSP+GRL QVEY +A+ +G T++ALRG + V+ +RK VP L VS Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRRG-TTAIALRGNECIVIGVERKNVPK--LQNVS 58 Query: 279 VTHLFALTDRIGCV-MTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQV 455 A+ D C+ G+ AD+R + +AR EA N + + + L R +A + Q Sbjct: 59 NFQKIAMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQK 118 Query: 456 YTQNAEMRPLGCSMMLIAYD-DEAGPCVYKTDPSGYYCSYKAVAAG 590 YTQ+ +RP G S ++ +D + P VY+T+P+G Y ++KA A G Sbjct: 119 YTQSGGVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIG 164 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 113 bits (272), Expect = 2e-26 Identities = 58/162 (35%), Positives = 90/162 (55%) Frame = +3 Query: 105 GFDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVT 284 G+D + FSP+GRL+Q EYA KA+ T + ++ D ++A ++ V +LL P Sbjct: 7 GYDLGL-FFSPDGRLFQAEYAYKAVENAS-TCIGIKCEDGVILALEKVVTSKLLKPRVNN 64 Query: 285 HLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQ 464 + ++ IG TG I D + V+RAR EA +W+ YGS IP ++ R+ + Q++T Sbjct: 65 RIGSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTC 124 Query: 465 NAEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAG 590 + +RP G + YD E GP +Y +P+G Y Y AAG Sbjct: 125 YSSVRPFGVMSFVATYDSE-GPHLYMVEPNGVYWGYNGAAAG 165 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 111 bits (266), Expect = 1e-25 Identities = 58/168 (34%), Positives = 99/168 (58%), Gaps = 6/168 (3%) Frame = +3 Query: 108 FDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTH 287 +DR + FSPEGRL+QVEYA++AI G T++ ++ DA V+ ++++ L++ SV Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLGS-TAIGVKTKDAVVLGVEKRLTSPLMESHSVEK 66 Query: 288 LFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQN 467 LF + IGC ++G+ AD+R+ ++ AR + N ++ Y + + I D++ + + Sbjct: 67 LFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEG 126 Query: 468 AE------MRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAGA 593 + RP G + +LIA DE GP +Y ++PSG Y Y+A A G+ Sbjct: 127 EDGEERIMSRPFGVA-LLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGS 173 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 92.3 bits (219), Expect = 5e-20 Identities = 55/164 (33%), Positives = 86/164 (52%) Frame = +3 Query: 108 FDRHITIFSPEGRLYQVEYALKAINQGGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTH 287 +D T +SP+GRL+QVEYAL+AI QG T + T A +VA +R + L Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEE--LSSYQ-KK 62 Query: 288 LFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQN 467 L + D IG + G+ D+R + EA + + + IPV L ++A+ +Q+ TQ Sbjct: 63 LIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQE 122 Query: 468 AEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYKAVAAGAKT 599 RP G ++I Y DE+GP + + PSG Y + G+++ Sbjct: 123 YGRRPYGVGFLVIGY-DESGPHLLEFQPSGLVLEYLGTSMGSRS 165 >SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1958 Score = 29.1 bits (62), Expect = 0.53 Identities = 15/62 (24%), Positives = 25/62 (40%) Frame = +3 Query: 327 GMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLI 506 GM +R+ Q +E +W Y + V LCR + ++ N + + LI Sbjct: 606 GMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLI 665 Query: 507 AY 512 Y Sbjct: 666 LY 667 >SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 576 Score = 25.4 bits (53), Expect = 6.5 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = -2 Query: 381 WQPRSELVVLETGYQQSSR 325 WQP S + L TGY ++R Sbjct: 205 WQPDSSQIFLTTGYDSTAR 223 >SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 630 Score = 25.0 bits (52), Expect = 8.6 Identities = 20/90 (22%), Positives = 38/90 (42%), Gaps = 1/90 (1%) Frame = +3 Query: 276 SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADIS-Q 452 ++ +L+ +I CV AD Q QR + + + G P HI I + S + Sbjct: 509 NLLNLYPDLQKIPCVFAATKADLDRQQQRYPVQPDEFTKQLGLPSPTHISTAAIWNTSKE 568 Query: 453 VYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 542 + Q AE S ++ ++++ Y+ Sbjct: 569 FFIQLAESAQYPASSIIRIPEEDSNKTNYQ 598 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,350,443 Number of Sequences: 5004 Number of extensions: 44186 Number of successful extensions: 122 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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