Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmov10l22
         (590 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family prote...   100   8e-22
At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote...    58   4e-09
At3g42320.1 68416.m04373 hypothetical protein various predicted ...    31   0.57 
At5g35753.1 68418.m04282 expressed protein                             29   1.8  
At2g36380.1 68415.m04464 ABC transporter family protein related ...    29   2.3  
At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containi...    28   4.1  
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati...    28   4.1  
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati...    28   4.1  
At2g31130.1 68415.m03801 expressed protein                             28   5.4  
At4g29400.1 68417.m04199 expressed protein ; expression supporte...    27   7.1  
At3g30560.1 68416.m03867 hypothetical protein                          27   7.1  
At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containi...    27   9.4  

>At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family protein 
           contains InterPro accession IPR002728: Diphthamide
           synthesis DPH2 protein
          Length = 491

 Score =  100 bits (239), Expect = 8e-22
 Identities = 47/135 (34%), Positives = 76/135 (56%), Gaps = 7/135 (5%)
 Frame = +3

Query: 138 TNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRI------EVDLYIMGDTS 299
           + ++I  T ++I  K F ++ LQFPD+LL+ S KV   +K +       EV  ++M DT+
Sbjct: 7   SKYEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTT 66

Query: 300 YASCCIDSVAAMHVQANGIVHYGHKCFSKTDI-PVYMVLPKEKLDFDAAINTLKEKYDSD 476
           Y SCCID V A+H+ +  +VHYG  C S T + P + V  K  ++  + +  L + Y S 
Sbjct: 67  YGSCCIDEVGALHIDSECVVHYGQTCLSPTSVLPAFFVFGKASINVSSCVKHLID-YASK 125

Query: 477 DNMKLCLFYGAEFEH 521
            +  + + YG E+ H
Sbjct: 126 SDKPIMILYGLEYAH 140


>At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein 
           contains InterPro accession IPR002728: Diphthamide
           synthesis DPH2 protein
          Length = 453

 Score = 58.0 bits (134), Expect = 4e-09
 Identities = 32/119 (26%), Positives = 58/119 (48%), Gaps = 5/119 (4%)
 Frame = +3

Query: 135 ITNFDIKETCKW-IERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVD-LYIMGDTSYAS 308
           I +F++ + C W I+     ++ LQ P+ LL  +  +             +++GD +Y +
Sbjct: 46  IYHFEVHK-CVWRIKTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGA 104

Query: 309 CCIDSVAAMHVQANGIVHYGHKC---FSKTDIPVYMVLPKEKLDFDAAINTLKEKYDSD 476
           CC+D  +A  + A+ ++HYGH C      T IP   V  + ++D    +NT+     SD
Sbjct: 105 CCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHLNLASD 163


>At3g42320.1 68416.m04373 hypothetical protein various predicted
           Helicases, Arabidopsis thaliana
          Length = 541

 Score = 31.1 bits (67), Expect = 0.57
 Identities = 31/121 (25%), Positives = 53/121 (43%), Gaps = 5/121 (4%)
 Frame = +3

Query: 159 TC--KWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVDL---YIMGDTSYASCCIDS 323
           TC  KW E  +F K      +D  ++ C+++K   D +  DL   Y++G T   SC    
Sbjct: 166 TCNPKWHEIVRFYKARNLKSEDRPDIICQIFKMKLDNLMYDLTTKYLLGKT--VSC---- 219

Query: 324 VAAMHVQANGIVHYGHKCFSKTDIPVYMVLPKEKLDFDAAINTLKEKYDSDDNMKLCLFY 503
           +  +  Q  G++H     F      +Y     +K+   A I   K+K +    +K C+ +
Sbjct: 220 MYTIEFQKRGLLHAHILLFLHATNKLYTAEDTDKV-ITAEIPDKKKKLELYVLVKDCMIH 278

Query: 504 G 506
           G
Sbjct: 279 G 279


>At5g35753.1 68418.m04282 expressed protein
          Length = 592

 Score = 29.5 bits (63), Expect = 1.8
 Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
 Frame = +3

Query: 111 KTEGYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLL-EVSCKV 242
           K +GY  +    D +E+ KWI R++  +   Q P   L E +C V
Sbjct: 524 KVDGYKTVYCRRDKEESKKWIPRREMLRFSHQVPSRFLKEETCGV 568


>At2g36380.1 68415.m04464 ABC transporter family protein related to
            multi drug resistance proteins and P-glycoproteins
          Length = 1453

 Score = 29.1 bits (62), Expect = 2.3
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
 Frame = -1

Query: 167  FACFLYIKIG-DYIVITFSFIYCY-LTCYTYF 78
            +   LY  IG D+ V+ F + Y Y LTC+ YF
Sbjct: 1294 YTLILYSMIGYDWTVVKFFWFYYYMLTCFVYF 1325


>At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile:PF00806 Pumilio-family RNA
           binding domains
          Length = 861

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 21/83 (25%), Positives = 40/83 (48%), Gaps = 5/83 (6%)
 Frame = +3

Query: 42  VKNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKW---IERKKFAKVCLQ-- 206
           ++ K+ N   E+K  + REI  +  +   D+  N+ I++  ++    +RK+ A   +   
Sbjct: 550 IQQKLENCKPEEKAAVFREILPHACKLMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQI 609

Query: 207 FPDDLLEVSCKVYKEIKDRIEVD 275
            P  L    C+V ++  D IE D
Sbjct: 610 VPLSLQMYGCRVIQKALDVIEPD 632


>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
           strong similarity to SP|P20115 Citrate synthase,
           mitochondrial precursor  {Arabidopsis thaliana};
           contains Pfam profile PF00285: Citrate synthase
          Length = 474

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 18/67 (26%), Positives = 30/67 (44%)
 Frame = +3

Query: 360 HYGHKCFSKTDIPVYMVLPKEKLDFDAAINTLKEKYDSDDNMKLCLFYGAEFEHCNENIT 539
           H G    + T   V   L    L F AA+N L        N ++ L+  +  E C E+I+
Sbjct: 272 HEGGNVSAHTGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGEDIS 331

Query: 540 RKWFQKY 560
           ++  ++Y
Sbjct: 332 KEQLKEY 338


>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
           strong similarity to SP|P20115 Citrate synthase,
           mitochondrial precursor  {Arabidopsis thaliana};
           contains Pfam profile PF00285: Citrate synthase
          Length = 473

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 18/67 (26%), Positives = 30/67 (44%)
 Frame = +3

Query: 360 HYGHKCFSKTDIPVYMVLPKEKLDFDAAINTLKEKYDSDDNMKLCLFYGAEFEHCNENIT 539
           H G    + T   V   L    L F AA+N L        N ++ L+  +  E C E+I+
Sbjct: 271 HEGGNVSAHTGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGEDIS 330

Query: 540 RKWFQKY 560
           ++  ++Y
Sbjct: 331 KEQLKEY 337


>At2g31130.1 68415.m03801 expressed protein
          Length = 419

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 14/39 (35%), Positives = 23/39 (58%)
 Frame = +3

Query: 150 IKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRI 266
           ++E+C  ++R +F  V   FPD +     K YK+I+D I
Sbjct: 333 VEESCILVDRDEFHSV---FPDKMENDKHKPYKKIRDAI 368


>At4g29400.1 68417.m04199 expressed protein ; expression supported
           by MPSS
          Length = 288

 Score = 27.5 bits (58), Expect = 7.1
 Identities = 15/40 (37%), Positives = 20/40 (50%)
 Frame = +3

Query: 420 EKLDFDAAINTLKEKYDSDDNMKLCLFYGAEFEHCNENIT 539
           E L  D  IN L +       +K  +F G+EFE   EN+T
Sbjct: 238 EPLILDVLINALTQISSDYVGIKKVVFGGSEFESWKENMT 277


>At3g30560.1 68416.m03867 hypothetical protein
          Length = 1473

 Score = 27.5 bits (58), Expect = 7.1
 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
 Frame = +3

Query: 120 GYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVDL---YIMG 290
           G+ D+   F      KW +  +F K      +D  ++ C++YK   + +  DL   +I G
Sbjct: 490 GFPDLFITFTCNS--KWPKITRFVKERKLNAEDKPDIICRIYKMKLENLMDDLTKNHIFG 547

Query: 291 DTSYASCCID 320
            T  A+  I+
Sbjct: 548 KTKLATYTIE 557


>At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile:PF00806 Pumilio-family RNA
           binding domains
          Length = 852

 Score = 27.1 bits (57), Expect = 9.4
 Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 5/82 (6%)
 Frame = +3

Query: 45  KNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKW---IERKKFAKVCLQ--F 209
           + K+ N   E+K  + REI  +  +   D+  N+ I++  ++    +RK+ A   +    
Sbjct: 542 QQKLENCKPEEKAAVFREILPHACKLMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQIV 601

Query: 210 PDDLLEVSCKVYKEIKDRIEVD 275
           P  L    C+V ++  D IE D
Sbjct: 602 PLSLQMYGCRVIQKALDVIEPD 623


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,745,124
Number of Sequences: 28952
Number of extensions: 266605
Number of successful extensions: 679
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 663
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 678
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1171109464
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)