BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10k02 (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 198 5e-52 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 102 4e-23 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 93 3e-20 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 93 3e-20 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 93 3e-20 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 85 7e-18 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 79 8e-16 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 77 2e-15 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 73 3e-14 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 66 3e-12 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 64 2e-11 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 63 4e-11 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 33 0.030 SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 29 0.48 SPBC19F8.06c |meu22||amino acid permease, unknown 11|Schizosacch... 27 2.6 SPAC328.08c |||tubulin specific chaperone cofactor C |Schizosacc... 26 4.5 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 7.9 SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces pomb... 25 7.9 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 198 bits (484), Expect = 5e-52 Identities = 93/182 (51%), Positives = 124/182 (68%) Frame = +1 Query: 76 MIKLFSLKQQKKDEEGSTRGGGSQKKASAAQLRITKDLNELNLPKTCNTEFPDPDDLLNF 255 M K++ LK+++ +E + G S AQ+RI KD+ +L +P T +T +PDP L Sbjct: 1 MRKIWELKKKEAQKEKNASG------ISPAQIRIQKDVTDLEIPSTMSTSWPDPIKLNVL 54 Query: 256 KLIICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWK 435 L I PDEG+Y+GGKF F ++ NYPH+PPKVKC +YHPNID+EGNVCLNILR+DW Sbjct: 55 HLEIRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWN 114 Query: 436 PVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMRGGYVGTSYFER 615 PVL +NSI+ GLQ+LFL PN EDPLNKEAA +L + + F V+ AM+GG V F+ Sbjct: 115 PVLNLNSILVGLQFLFLSPNAEDPLNKEAAADLHKDPQGFASRVRTAMKGGLVNGISFDN 174 Query: 616 CL 621 + Sbjct: 175 VM 176 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 102 bits (245), Expect = 4e-23 Identities = 50/132 (37%), Positives = 78/132 (59%), Gaps = 3/132 (2%) Frame = +1 Query: 172 RITKDLNELNL--PKTCNTEFPDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHE 342 RI ++L +L P +C+ P DDL +++ I P + Y GG F S +YP + Sbjct: 5 RINRELADLGKDPPSSCSAG-PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63 Query: 343 PPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEA 522 PPKV T +YHPNI+ G++CL+ILR+ W P LT++ ++ + L +PNP+DPL E Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 123 Query: 523 ADELQSNRRLFE 558 A +++R +E Sbjct: 124 AHVYKTDRSRYE 135 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 93.5 bits (222), Expect = 3e-20 Identities = 47/122 (38%), Positives = 71/122 (58%), Gaps = 3/122 (2%) Frame = +1 Query: 172 RITKDLNELNLPKTCN-TEFPDPD-DLLNFK-LIICPDEGFYRGGKFVFSFKVGPNYPHE 342 R+ +L L + T + FPD D +LL++ I P + +Y G KF S NYP+ Sbjct: 32 RLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYS 91 Query: 343 PPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEA 522 PP + + ++HPN+D+ GN+CL+IL++ W V V +I+ LQ L EPN PLN +A Sbjct: 92 PPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQA 151 Query: 523 AD 528 A+ Sbjct: 152 AE 153 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 93.5 bits (222), Expect = 3e-20 Identities = 41/98 (41%), Positives = 63/98 (64%), Gaps = 1/98 (1%) Frame = +1 Query: 271 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILRED-WKPVLT 447 P + Y GGKF FS K +YP +PP ++ T +YHPN D EGNVCL IL++ +KP + Sbjct: 46 PSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIK 105 Query: 448 VNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQ 561 + S++ + L EPNP+DPL A++ +++R F++ Sbjct: 106 LRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDK 143 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 93.1 bits (221), Expect = 3e-20 Identities = 45/126 (35%), Positives = 68/126 (53%), Gaps = 2/126 (1%) Frame = +1 Query: 172 RITKDLNELNL-PKTCNTEFPDPDDLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEP 345 RI K++ L P P D+L FK+ + P + Y GGKF + YP P Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMP 65 Query: 346 PKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAA 525 P V+ T +YHPN+D G +CL+ L++DW P L + +++ +Q L PNP+DPL+ + A Sbjct: 66 PNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVA 125 Query: 526 DELQSN 543 + N Sbjct: 126 KIWKEN 131 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 85.4 bits (202), Expect = 7e-18 Identities = 44/131 (33%), Positives = 70/131 (53%), Gaps = 3/131 (2%) Frame = +1 Query: 172 RITKDLNELNLPKTCNTE-FPDPDDLLNFKLIICPDEGF-YRGGKFVFSFKVGPNYPHEP 345 RI K+L ++ K + + DD+ + K + EG Y GG FV ++ +YP P Sbjct: 8 RIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRP 67 Query: 346 PKVKCETAVYHPNIDLE-GNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEA 522 PK+ +T +YHPN+ + G +CL+IL++ W PV T+ S + LQ L P P +P + + Sbjct: 68 PKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQV 127 Query: 523 ADELQSNRRLF 555 A N + F Sbjct: 128 AQVYLQNYQQF 138 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 78.6 bits (185), Expect = 8e-16 Identities = 48/159 (30%), Positives = 81/159 (50%), Gaps = 16/159 (10%) Frame = +1 Query: 154 ASAAQLRITKDLNEL--NLPKTCNTEFPDPDDLLNFK-LIICPDEGFYRGGKFVFSFKVG 324 +SA++ + K L E+ N P+ + D + ++ +II P++ Y GG F + Sbjct: 3 SSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFP 62 Query: 325 PNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR-------------EDWKPVLTVNSIVY 465 +YP PPK+K T ++HPN+ G VC++IL E W PV + +I+ Sbjct: 63 QDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILI 122 Query: 466 GLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMR 582 + + PN E P N +AA E + N + F++ V+R +R Sbjct: 123 SVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVR 161 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 77.4 bits (182), Expect = 2e-15 Identities = 40/148 (27%), Positives = 76/148 (51%), Gaps = 2/148 (1%) Frame = +1 Query: 154 ASAAQLRITKDLNELNL-PKTCNTEFPDPDDLLNFK-LIICPDEGFYRGGKFVFSFKVGP 327 ++ A+ R+ +D + P + P D+++ + +II P + + G F Sbjct: 2 STTARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDE 61 Query: 328 NYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDP 507 YP++PP VK + ++HPN+ G +CL+IL+ W P V +I+ +Q L +PN P Sbjct: 62 QYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASP 121 Query: 508 LNKEAADELQSNRRLFEQHVQRAMRGGY 591 N EAA + N++ + + V++ + + Sbjct: 122 ANAEAAQLHRENKKEYVRRVRKTVEDSW 149 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 73.3 bits (172), Expect = 3e-14 Identities = 38/117 (32%), Positives = 62/117 (52%), Gaps = 3/117 (2%) Frame = +1 Query: 241 DLLNFKL-IICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNI 417 DL+N+K+ I + + GG + + YP PPK + ++HPN+ G VCL+I Sbjct: 37 DLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSI 96 Query: 418 LRED--WKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMR 582 L E+ WKP +T+ I+ G+Q L +PN P EA + ++ +E+ V+ R Sbjct: 97 LNEEEGWKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVRAQAR 153 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 66.5 bits (155), Expect = 3e-12 Identities = 35/102 (34%), Positives = 53/102 (51%), Gaps = 2/102 (1%) Frame = +1 Query: 241 DLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLN 414 D+ ++K +I P E Y GG++V V YP PP V +T + HPNI G VC++ Sbjct: 33 DMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMD 92 Query: 415 ILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQS 540 IL+ W P ++ S + L + PLN +AA L++ Sbjct: 93 ILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRT 134 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 63.7 bits (148), Expect = 2e-11 Identities = 32/87 (36%), Positives = 46/87 (52%), Gaps = 2/87 (2%) Frame = +1 Query: 271 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLNILREDWKPVL- 444 P E Y GG + ++ YP + P + ++HPNID L G+VCL+++ + W P+ Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFD 98 Query: 445 TVNSIVYGLQYLFLEPNPEDPLNKEAA 525 +N L L PN DPLN EAA Sbjct: 99 MINIFEVFLPQLLRYPNASDPLNGEAA 125 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 62.9 bits (146), Expect = 4e-11 Identities = 47/160 (29%), Positives = 73/160 (45%), Gaps = 16/160 (10%) Frame = +1 Query: 151 KASAAQLRITKDLNEL--NLPKTCNTEFPDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKV 321 KA A + R+ K+ EL N P + DD + +I PD + GG + + K Sbjct: 3 KAMALR-RLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKF 61 Query: 322 GPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR-------------EDWKPVLTVNSIV 462 +YP PP +K E +HPN+ +G VC++IL E W PV +V I+ Sbjct: 62 PSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKIL 121 Query: 463 YGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMR 582 + + EPN E N +A + +R + + V+R R Sbjct: 122 LSVMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLAR 161 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 33.5 bits (73), Expect = 0.030 Identities = 22/85 (25%), Positives = 40/85 (47%), Gaps = 4/85 (4%) Frame = +1 Query: 229 PDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNV 405 P +++L + II P + Y GG++ + P+YP +PP ++ T + Sbjct: 29 PATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRL 86 Query: 406 CLNIL---REDWKPVLTVNSIVYGL 471 CL+ + W P V++I+ GL Sbjct: 87 CLSFSDFHPKSWNPSWMVSTILVGL 111 >SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 509 Score = 29.5 bits (63), Expect = 0.48 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +2 Query: 80 LNFFH*NNRKKMKRVLPEEEDHKKKHQQP 166 + +F NN K ++R+L E ED KH +P Sbjct: 235 IRYFKHNNMKDLERILQELEDDFVKHNRP 263 >SPBC19F8.06c |meu22||amino acid permease, unknown 11|Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 27.1 bits (57), Expect = 2.6 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +1 Query: 505 PLNKEAADELQSNRRLFEQHVQRAMRGGYVGTSYF 609 PL E +LQ R+L +H+Q GG VGT F Sbjct: 49 PLQPEDTQDLQ--RKLKPRHMQMIAIGGCVGTGLF 81 >SPAC328.08c |||tubulin specific chaperone cofactor C |Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/59 (22%), Positives = 26/59 (44%) Frame = +2 Query: 476 IYSWNQTLKIPSTRKQLTSSKATGDFSNNTFRELCEAATSAHLISNDASNENLHTCKKC 652 +Y N TL + + + Q +N + ++ S ++ ++ S+ NLH KC Sbjct: 114 VYFENDTLYLANLKNQNIGDNVIPYPNNKAVKVSAKSLRSCNISISNCSSVNLHNATKC 172 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/43 (25%), Positives = 22/43 (51%) Frame = -1 Query: 266 IINLKFNRSSGSGNSVLHVFGRFNSFKSFVIRNWAADAFFCDP 138 II+L + +S + N+ ++FG F ++ + W+ F P Sbjct: 214 IISLAASSTSETRNTGSYIFGNFENYSGWTNMGWSFILCFTTP 256 >SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 25.4 bits (53), Expect = 7.9 Identities = 18/46 (39%), Positives = 23/46 (50%) Frame = +2 Query: 515 RKQLTSSKATGDFSNNTFRELCEAATSAHLISNDASNENLHTCKKC 652 RK + S TGD ++ F EAAT A L D+ N+ T KC Sbjct: 157 RKYMECSSLTGDGVDDVF----EAATRAALTVRDSENDKSST--KC 196 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,625,605 Number of Sequences: 5004 Number of extensions: 56257 Number of successful extensions: 216 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 199 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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