BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10j02 (690 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC530.15c ||SPBC661.01|spermidine family transporter |Schizosa... 27 2.6 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 27 3.4 SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces ... 26 4.5 >SPBC530.15c ||SPBC661.01|spermidine family transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 516 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/25 (40%), Positives = 13/25 (52%) Frame = +2 Query: 590 LILPIRVFWLLWTNILGPWFFQEAP 664 ++ PI +FWL WT PW P Sbjct: 394 IVFPIGIFWLAWTGNY-PWIHWIVP 417 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/58 (22%), Positives = 28/58 (48%) Frame = +1 Query: 478 FRFEHGGWNGRTYQGYCNTLIYNSSFSIDKQLLLAIASNFTNKSILAIVDQYTWSLVF 651 F+ E+ W+ G T++ N + S D + + + +NK +++ +T L+F Sbjct: 512 FQVEYSSWSESFLAGKRLTVVKNQAVSNDDNINMVNIPDGSNKKYMSLRQHFTAVLLF 569 >SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 306 Score = 26.2 bits (55), Expect = 4.5 Identities = 8/38 (21%), Positives = 22/38 (57%) Frame = +2 Query: 290 RNMSMAATSFYGIITALFYYENISNWVLFFNVLVLIIH 403 +N+ +A+ F + + YY W++ F ++++++H Sbjct: 81 KNVFSSASFFREVSVRMVYYVRYIQWLINFPLIIVMLH 118 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,962,002 Number of Sequences: 5004 Number of extensions: 63690 Number of successful extensions: 129 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 124 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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