BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10i04 (576 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 87 1e-17 At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 83 1e-16 At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 81 4e-16 At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 79 2e-15 At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 77 6e-15 At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 75 3e-14 At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 75 3e-14 At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 72 2e-13 At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 69 2e-12 At5g56860.1 68418.m07095 zinc finger (GATA type) family protein ... 31 0.73 At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 1.3 At4g19865.1 68417.m02911 kelch repeat-containing F-box family pr... 29 1.7 At3g50780.1 68416.m05561 expressed protein 29 2.2 At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 2.2 At3g10630.1 68416.m01278 glycosyl transferase family 1 protein c... 29 2.9 At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 28 3.9 At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 3.9 At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 5.1 At3g62090.1 68416.m06976 basic helix-loop-helix (bHLH) protein, ... 27 6.8 At5g39770.1 68418.m04817 repair endonuclease family protein cont... 27 9.0 At3g57570.1 68416.m06410 expressed protein 27 9.0 At2g13950.1 68415.m01550 DC1 domain-containing protein contains ... 27 9.0 >At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from [Arabidopsis thaliana] Length = 307 Score = 86.6 bits (205), Expect = 1e-17 Identities = 47/147 (31%), Positives = 73/147 (49%), Gaps = 8/147 (5%) Frame = +1 Query: 151 DANNLPEDAPPHVPPSAEKRPWKIV---WRNV--ILF---FILHVGGVYGGYLFLFKAMW 306 + N+ + P +KR W WR + + F F +H + + F + A+W Sbjct: 8 EENHQKNPSTPAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALW 67 Query: 307 RTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHR 486 T +F Y GLGIT HR AH+S+K L LL +A Q +DW HR Sbjct: 68 VTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHR 123 Query: 487 MHHKYSETDADPHNATRGFFFSHIGWL 567 HH++++++ DPH+ GF+FSH+ W+ Sbjct: 124 YHHQFTDSERDPHSPKEGFWFSHLLWI 150 >At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180 GI:2970034 from [Arabidopsis thaliana] Length = 305 Score = 83.4 bits (197), Expect = 1e-16 Identities = 44/144 (30%), Positives = 67/144 (46%), Gaps = 3/144 (2%) Frame = +1 Query: 145 EADANNLPEDAPPHVPPSAEKRPWKIVWRNVILFFILHVGGVYGGYLFL---FKAMWRTS 315 E + NN A ++ W+ W+ L + ++ +L L F W Sbjct: 7 EKEENNKKMAADKAEMGRKKRAMWERKWKR--LDIVKAFASLFVHFLCLLAPFNFTWPAL 64 Query: 316 IFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHRMHH 495 A+ +Y GLGIT HR AH+S+K L +A Q +DW HR HH Sbjct: 65 RVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 124 Query: 496 KYSETDADPHNATRGFFFSHIGWL 567 +++++D DPH+ GF+FSH+ WL Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWL 148 >At1g06360.1 68414.m00672 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 81.4 bits (192), Expect = 4e-16 Identities = 43/127 (33%), Positives = 63/127 (49%), Gaps = 3/127 (2%) Frame = +1 Query: 196 SAEKRPW---KIVWRNVILFFILHVGGVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAG 366 S EKRP+ + W ++I + + + L F W F LY + L IT Sbjct: 17 SKEKRPYIHREWSWADIIRALTV-INVHFLCLLAPFNYKWEALRFGFVLYALTSLSITFS 75 Query: 367 AHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSETDADPHNATRGFF 546 HR AH+S+K L L F A Q +DW HR HH+++++D DPH+ GF+ Sbjct: 76 YHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFW 135 Query: 547 FSHIGWL 567 FSH+ W+ Sbjct: 136 FSHVWWI 142 >At3g15850.1 68416.m02005 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 371 Score = 79.0 bits (186), Expect = 2e-15 Identities = 36/93 (38%), Positives = 51/93 (54%), Gaps = 1/93 (1%) Frame = +1 Query: 292 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 468 F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180 Query: 469 WARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 W HR HH++ ++D DPH+ GF+FSH+ W+ Sbjct: 181 WVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 213 >At3g15870.1 68416.m02007 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 292 Score = 77.4 bits (182), Expect = 6e-15 Identities = 35/98 (35%), Positives = 55/98 (56%) Frame = +1 Query: 274 GGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQ 453 G L +A W +F +++ +G+ IT HR +H+S+ L L +AFQ Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQ 98 Query: 454 DAVVDWARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 ++W +HR HHK+ ET DPH+ T+GF+FSH+ W+ Sbjct: 99 GDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWI 136 >At1g06090.1 68414.m00638 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana] Length = 299 Score = 75.4 bits (177), Expect = 3e-14 Identities = 32/92 (34%), Positives = 46/92 (50%) Frame = +1 Query: 292 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 471 F W F + L + + L IT HR HKS+K L A Q +DW Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110 Query: 472 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At1g06120.1 68414.m00641 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported by cDNA:gi_12083275_gb_AF332434.1_AF332434 Length = 299 Score = 74.9 bits (176), Expect = 3e-14 Identities = 37/115 (32%), Positives = 55/115 (47%), Gaps = 1/115 (0%) Frame = +1 Query: 226 WRNVILFFILHVGGVYGGYLFL-FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKA 402 W V + + VG V+ L F W FA + + + L IT HR H+S+K Sbjct: 28 WTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKL 87 Query: 403 RLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 L A Q +DW HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 88 PKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At1g06100.1 68414.m00639 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 72.1 bits (169), Expect = 2e-13 Identities = 32/92 (34%), Positives = 47/92 (51%) Frame = +1 Query: 292 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 471 F W F I L + + L IT HR H+S+K L +A Q +DW Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110 Query: 472 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVLWI 142 >At1g06350.1 68414.m00671 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 300 Score = 68.9 bits (161), Expect = 2e-12 Identities = 31/92 (33%), Positives = 45/92 (48%) Frame = +1 Query: 292 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 471 F W F + L+ + L IT HR +H+S+K L A Q +DW Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111 Query: 472 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 567 HR HH+++++D DPH+ G FSHI W+ Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLFSHILWI 143 >At5g56860.1 68418.m07095 zinc finger (GATA type) family protein similar to unknown protein (pir |T04270) Length = 398 Score = 30.7 bits (66), Expect = 0.73 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = -3 Query: 286 TSTLHKHHRHAE*RTESHFSTQSSKVSSL 200 +S+LH HH H + S+ S+ SS +SSL Sbjct: 29 SSSLHHHHHHHHHQVPSNSSSSSSSISSL 57 >At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) identical to metal transporter Nramp4 [Arabidopsis thaliana] gi|6468014|gb|AAF13279; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563 Length = 512 Score = 29.9 bits (64), Expect = 1.3 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = +1 Query: 265 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 372 G GY ++ MW T+I + L + LG+ G H Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114 >At4g19865.1 68417.m02911 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 393 Score = 29.5 bits (63), Expect = 1.7 Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 3/94 (3%) Frame = +1 Query: 175 APPHVPPSAEKRPWKIVWRNVILFFILHVGGVYGGYLFLFKAMWRTSIFAIFLYLC-SGL 351 +PPH PPS+ ++ +++ + + +Y L L +R+ + + LY S L Sbjct: 17 SPPHSPPSSSSPSLSLLPEEIVVHCLARISRLYYPTLSLVSKSFRSILSSTELYATRSHL 76 Query: 352 GITAGAHR--LWAHKSYKARLPLRILLTIFNTIA 447 G T LW SY+ LR+L+ T+A Sbjct: 77 GSTEQCVYLCLW-DPSYQFPQWLRLLVNPNRTLA 109 >At3g50780.1 68416.m05561 expressed protein Length = 520 Score = 29.1 bits (62), Expect = 2.2 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 2/68 (2%) Frame = +3 Query: 111 PKETGGFVG--IVRSRRQ*PARRCTAPCTSLSREETLEDCVEKCDSVLYSACRWCLWRVL 284 PKET + ++RS+ + R + L RE+ + + + +YS+C+ CL VL Sbjct: 270 PKETLSRIIELVLRSKEEKSRREMKSIVLKLLREQNGANVADNFNDTIYSSCQTCLDSVL 329 Query: 285 VPFQSHVE 308 F+ E Sbjct: 330 SLFKQASE 337 >At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) identical to metal transporter Nramp3 [Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563 Length = 509 Score = 29.1 bits (62), Expect = 2.2 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = +1 Query: 265 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 372 G GY L+ MW T++ + L + LG+ G H Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118 >At3g10630.1 68416.m01278 glycosyl transferase family 1 protein contains Pfam glycosyl transferase, group 1 family protein domain PF00534; C-terminal portion similar to mannosyltransferase GB:BAA28328 [Escherichia coli] Length = 487 Score = 28.7 bits (61), Expect = 2.9 Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 3/27 (11%) Frame = +1 Query: 190 PPSAEKRPWK---IVWRNVILFFILHV 261 PP KRPWK IV+ + ILF +L + Sbjct: 4 PPDRSKRPWKFSTIVYSSSILFLLLSI 30 >At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / histidine--tRNA ligase, putative similar to SP|P12081 Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 479 Score = 28.3 bits (60), Expect = 3.9 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = -3 Query: 109 EAFSLIQNIFKYCKCVVLQTAVFKKHAT 26 +AFS+IQN+FK L T VF+ T Sbjct: 61 KAFSIIQNVFKRHGATALDTPVFELRET 88 >At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative (NRAMP2) similar to metal transporter Nramp3 [Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563 Length = 530 Score = 28.3 bits (60), Expect = 3.9 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = +1 Query: 265 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 372 G GY L+ MW T++ + L + +G+ G H Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130 >At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Lycopersicon esculentum] GI:7381227; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 468 Score = 27.9 bits (59), Expect = 5.1 Identities = 11/34 (32%), Positives = 16/34 (47%) Frame = +1 Query: 130 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWR 231 S+ ++ D +P D P P S KR W +R Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 157 >At3g62090.1 68416.m06976 basic helix-loop-helix (bHLH) protein, putative very strong similarity to PIF3 like basic Helix Loop Helix protein 2 (PIL2) [Arabidopsis thaliana] GI:22535494 Length = 346 Score = 27.5 bits (58), Expect = 6.8 Identities = 22/74 (29%), Positives = 26/74 (35%) Frame = +3 Query: 336 LVFGIGYHSWRAQTLGTQVL*SSAATENITHHIQYYRFSGCRSGLGP*SSHAPQIFRDRC 515 L G+G Q L VL A I RF GL P + AP I + C Sbjct: 251 LAMGVGMQMGEQQFLPAHVL--GAGLPGINDSADMLRFLN-HPGLMPMQNSAPFIPTENC 307 Query: 516 GPPQRNPRILLFPH 557 P P FP+ Sbjct: 308 SPQSVPPSCAAFPN 321 >At5g39770.1 68418.m04817 repair endonuclease family protein contains Pfam PF02732 : ERCC4 domain; similar to MUS81 endonuclease (GI:16755674) [Mus musculus]; similar to repair endonuclease (TIGR:At5g41150) [Arabidopsis thaliana] Length = 1242 Score = 27.1 bits (57), Expect = 9.0 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = -3 Query: 142 TIPTKPPVSFGEAFSLIQNI 83 TIP PP+ FGEAF + ++ Sbjct: 979 TIPRLPPLRFGEAFEEVYDV 998 >At3g57570.1 68416.m06410 expressed protein Length = 1057 Score = 27.1 bits (57), Expect = 9.0 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +2 Query: 482 IACTTNIQRPMRTPTTQPEDSSFPT 556 +A T NI + PT+ PEDS+ T Sbjct: 232 VALTPNISEKKKRPTSLPEDSNIET 256 >At2g13950.1 68415.m01550 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 517 Score = 27.1 bits (57), Expect = 9.0 Identities = 16/55 (29%), Positives = 26/55 (47%) Frame = +3 Query: 204 EETLEDCVEKCDSVLYSACRWCLWRVLVPFQSHVEDFYIRHIFVLVFGIGYHSWR 368 E T C++KC+ +L+ AC V P + H + + + V IGY +R Sbjct: 292 EGTYYSCLDKCEFILHEAC------VNAPRKKH-HPLHTHPLTLKVVSIGYDHYR 339 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,588,624 Number of Sequences: 28952 Number of extensions: 375715 Number of successful extensions: 1078 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 1040 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1077 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1121903184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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