SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmov10g12
         (669 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...   177   2e-45
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc...   150   2e-37
SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac...   103   3e-23
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    43   5e-05
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...    36   0.007
SPAC1805.05 |cki3||serine/threonine protein kinase Cki3|Schizosa...    29   0.80 
SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pomb...    27   1.8  
SPAC6C3.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    27   2.4  
SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces p...    27   2.4  
SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac...    26   4.3  
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo...    26   4.3  
SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizos...    26   5.6  
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy...    26   5.6  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    26   5.6  
SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb...    25   7.5  
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    25   7.5  
SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6 family|Schi...    25   7.5  
SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharom...    25   7.5  
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p...    25   9.9  
SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At...    25   9.9  
SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schi...    25   9.9  

>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score =  177 bits (430), Expect = 2e-45
 Identities = 81/143 (56%), Positives = 108/143 (75%), Gaps = 1/143 (0%)
 Frame = +1

Query: 76  MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAK-HTNFVEFRNFKIVY 252
           MI+FILIQNR GK RL+K+Y+ FDDDEK +L   +H +++ R+ K   NF+E+ N K+VY
Sbjct: 1   MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60

Query: 253 RRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFL 432
           RRYAGLYFC CVD  DN+L  LE IH FVE+L+ +F NVCELDL+FNFYKV  ++DE+ L
Sbjct: 61  RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEIIL 120

Query: 433 AGEIRETSQTKVLKQLLMLNSLE 501
            GEI E+++  VL+++  L  LE
Sbjct: 121 GGEIGESNKKSVLERIEALEKLE 143


>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score =  150 bits (363), Expect = 2e-37
 Identities = 69/135 (51%), Positives = 95/135 (70%)
 Frame = +1

Query: 79  IRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRR 258
           I+F L+ +R GK RLAKW+      E+ K+I +V ++V  R  K  NFVE++  KIVYRR
Sbjct: 3   IKFFLLVSRQGKVRLAKWFNTLSIKERAKIIRDVSSLVITRKPKMCNFVEYKGEKIVYRR 62

Query: 259 YAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAG 438
           YA L+F   ++ +DN L  LE IH FVE L++YF NVCELDL+FNF K Y V++E+ LAG
Sbjct: 63  YASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFEKAYYVMEELLLAG 122

Query: 439 EIRETSQTKVLKQLL 483
           E++E+S+T VL  +L
Sbjct: 123 ELQESSKTNVLSAVL 137


>SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 165

 Score =  103 bits (246), Expect = 3e-23
 Identities = 53/136 (38%), Positives = 81/136 (59%), Gaps = 4/136 (2%)
 Frame = +1

Query: 76  MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNF----K 243
           MI  + I N  GK RL K+Y   D+  +QKLI +++A V+ R     NF+E        +
Sbjct: 1   MIYAVFIFNNKGKPRLTKFYTPIDESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGKNR 60

Query: 244 IVYRRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDE 423
           I+YR+YA LYF   VD  ++ L  L+ I  FVE L+  F+NVCELDLVF F +++ ++ E
Sbjct: 61  IIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAILAE 120

Query: 424 MFLAGEIRETSQTKVL 471
           +   G + ET+  +++
Sbjct: 121 VVSGGLVLETNLNEIV 136


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 34/127 (26%), Positives = 57/127 (44%), Gaps = 1/127 (0%)
 Frame = +1

Query: 76  MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVH-AVVTVRDAKHTNFVEFRNFKIVY 252
           MI  + I N  G T + K    F  D K+ + E    A++T  D +H   V   +   +Y
Sbjct: 1   MISGLFIFNLKGDTLICK---TFRHDLKKSVTEIFRVAILTNTDYRHP-IVSIGSSTYIY 56

Query: 253 RRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFL 432
            ++  LY       N N +  LE + + ++ L  YF  + E  +  N   ++ ++DEM  
Sbjct: 57  TKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMID 116

Query: 433 AGEIRET 453
            G I+ T
Sbjct: 117 YGIIQTT 123


>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 35.5 bits (78), Expect = 0.007
 Identities = 20/83 (24%), Positives = 41/83 (49%)
 Frame = +1

Query: 247 VYRRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEM 426
           +Y  +  +Y      +N + +  L  +    +V  +YF  + E  +  NF  VY ++DE+
Sbjct: 58  IYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEI 117

Query: 427 FLAGEIRETSQTKVLKQLLMLNS 495
              G   +T++TK+L++ +   S
Sbjct: 118 MDFG-FPQTTETKILQEYITQTS 139


>SPAC1805.05 |cki3||serine/threonine protein kinase
           Cki3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 439

 Score = 28.7 bits (61), Expect = 0.80
 Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
 Frame = -3

Query: 493 SSASRVV*AL*FGMSP*SHQPKTFRLQPYTLYKN*RPSPTRKHYG-NIHLGPRQS 332
           S    V+ A+ FGM+     PKT   +PY  +K+   S T ++   N HLG  QS
Sbjct: 147 SKTENVIYAVDFGMAKQYRDPKTHVHRPYNEHKS--LSGTARYMSINTHLGREQS 199


>SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 494

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +3

Query: 15  GGVNPSLKVIFKKKAITKRENDKIHFD 95
           GGV  +  V+ KKK I K END+ + +
Sbjct: 334 GGVGSAFGVVGKKKQIYKGENDEFYVE 360


>SPAC6C3.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 269

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
 Frame = +1

Query: 286 VDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFY-KVYTVVDEMF 429
           ++ +DN+L YL         L  YF  +   + V N   K  TV+D ++
Sbjct: 111 INASDNSLAYLSKPEESYNFLTIYFSTLLSYEKVENLIKKSLTVIDSLY 159


>SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 464

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 11/31 (35%), Positives = 17/31 (54%)
 Frame = +1

Query: 289 DVNDNNLCYLEAIHNFVEVLNEYFHNVCELD 381
           D +DN+ C+     N +  LN YF N  ++D
Sbjct: 180 DKSDNSNCWRPIATNLLHRLNAYFQNEVKMD 210


>SPCC18B5.11c |cds1||replication checkpoint kinase
           Cds1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 17/52 (32%), Positives = 25/52 (48%)
 Frame = +1

Query: 328 HNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLL 483
           H  V   +E F N  EL +V  + +   ++D +   G I E     +LKQLL
Sbjct: 228 HPGVVQCHEIFENDDELFIVMEYVEGGDLMDFLIANGSIDEQDCKPLLKQLL 279


>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1183

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 8/25 (32%), Positives = 16/25 (64%)
 Frame = +3

Query: 66  KRENDKIHFDPEPSRQDSTCEMVYE 140
           +  + ++H +PEP  QDS  E++ +
Sbjct: 35  QESSSEVHANPEPDNQDSNAEILID 59


>SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 10/34 (29%), Positives = 17/34 (50%)
 Frame = +1

Query: 112 KTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKH 213
           K RL  WY  +    ++ L+E+  A +  +  KH
Sbjct: 141 KRRLDAWYFGYIPPAQESLLEDFEAKIEEQQHKH 174


>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 542

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 14/64 (21%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
 Frame = -1

Query: 297 IYIDTYTEVQTCIPPVYYLEISELDKVRVFRVS---DGDDCMHFFYQLLFFVIIKIHIPF 127
           +++D  ++   C   V Y +   ++ +RV+ V+   D   CM  F     +V+  +  P+
Sbjct: 59  LFVDPLSDGDACSRDVPYFQELSVNAIRVYAVNASLDHSACMQAFQDAGIYVLSDLAQPY 118

Query: 126 RKSS 115
              S
Sbjct: 119 EAIS 122


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 12/56 (21%), Positives = 26/56 (46%)
 Frame = +1

Query: 91  LIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRR 258
           L+ N  GK    +   ++D  +   LIE+ + + +        FVE+++  +  R+
Sbjct: 651 LLLNEMGKLFQVELNFSYDSPDVNLLIEQFYEITSYESNYDDAFVEYKDATVANRK 706


>SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 440

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 23/86 (26%), Positives = 35/86 (40%), Gaps = 6/86 (6%)
 Frame = +1

Query: 190 VTVRDAKHTNFVEFRNFKIVYRRYAGLYFCICVDVNDNNLCYLEAIHNF---VEVLNEYF 360
           VTV +    N      F   +   +     IC   NDNNL  L+  H     ++VL +Y 
Sbjct: 181 VTVHETGFGNIENLATFSTKFVALSSHKNYICGLTNDNNLYILQHSHQAGTKLKVLGKYH 240

Query: 361 HNVCELDLVFNFYKV---YTVVDEMF 429
            +  E  +  +F +    Y VV  +F
Sbjct: 241 VSSIEKQVAIHFQQSKEGYEVVHVVF 266


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = -2

Query: 350 FRTSTKLCMASR*HKLLSFTSTHIQKYRPAYLLYTIL 240
           F+T+T+LC        LSF+  H + + P++++ TIL
Sbjct: 80  FQTNTRLC--------LSFSDFHPKSWNPSWMVSTIL 108


>SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 984

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 23/92 (25%), Positives = 36/92 (39%), Gaps = 2/92 (2%)
 Frame = -2

Query: 521 KNNTNTYSKEFSIKSCLSTLVWDVSLISPAKNIXXXXXXXXXXXXXXSNSQT--LWKYSF 348
           KN    Y +EF  ++    L+  + L  PA                   S+T  L  Y+ 
Sbjct: 435 KNQFRVYLQEFDFEADKVNLISTIELELPATFDPTSLTVDEEYSNFLIGSRTGSLALYNI 494

Query: 347 RTSTKLCMASR*HKLLSFTSTHIQKYRPAYLL 252
             S  L +  R H+  + +S HI+  +  YLL
Sbjct: 495 GESNYLSVWRRIHEFDAISSIHIKSSKRDYLL 526


>SPAC959.03c |||U3 snoRNP-associated protein
           Utp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 520

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 15/49 (30%), Positives = 25/49 (51%)
 Frame = +1

Query: 127 KWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRRYAGLY 273
           K +++  D    KL+ E+H   TVRD K   F   + F +  ++Y  +Y
Sbjct: 122 KGHISAFDWRTGKLLTELHLRETVRDVKW--FHNHQYFAVAQKKYVYVY 168


>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 926

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -1

Query: 462 SLGCLPDLTSQKHFVYNRIHFIKIEDQVQLAN 367
           +L CLP  TS K   Y+R +  + ED ++ A+
Sbjct: 529 TLSCLPRYTSGKTQFYHRWNASRSEDALKFAS 560


>SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 3/26 (11%)
 Frame = -1

Query: 261 IPPVY---YLEISELDKVRVFRVSDG 193
           IPP+    YL+IS+L K +   VSDG
Sbjct: 106 IPPMVLHTYLDISDLSKNQTLSVSDG 131


>SPBC146.11c |mug97|meu33|meiotically upregulated gene
           Mug97|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 7/17 (41%), Positives = 13/17 (76%)
 Frame = +1

Query: 124 AKWYMNFDDDEKQKLIE 174
           ++WY+ FD D+  +L+E
Sbjct: 178 SRWYLPFDKDDDNQLVE 194


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,743,888
Number of Sequences: 5004
Number of extensions: 57081
Number of successful extensions: 205
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -