Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov10g05
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 94 1e-20
SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2 |Schizosacc... 70 2e-13
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 63 3e-11
SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 46 4e-06
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 29 0.75
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 26 5.3
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 25 7.0
SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 25 9.3
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 25 9.3
SPAC186.01 |||DIPSY family|Schizosaccharomyces pombe|chr 1|||Manual 25 9.3
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 94.3 bits (224), Expect = 1e-20
Identities = 47/128 (36%), Positives = 76/128 (59%), Gaps = 2/128 (1%)
Frame = +2
Query: 266 ERIKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQR 445
E+ ++K+A L+GY G Y+GMQ NP +TI ++ + ++ + R
Sbjct: 39 EKKPKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKVALAR 98
Query: 446 SSRTDKGVSAAEQVVSLKLPLEVN--VEEINKRLPETIKVFAVKRVTNRFNSKSKCNARS 619
++RTDKGV AA V+SLKL +E +E++N+ LP +I+++ V R N FN ++ C +R
Sbjct: 99 AARTDKGVHAAGNVISLKLIMEDEKLIEKVNEHLPPSIRLWDVIRTINSFNPRTYCESRI 158
Query: 620 YSYTLPTY 643
Y Y +PTY
Sbjct: 159 YEYMVPTY 166
>SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 451
Score = 70.1 bits (164), Expect = 2e-13
Identities = 39/126 (30%), Positives = 70/126 (55%), Gaps = 6/126 (4%)
Frame = +2
Query: 284 KMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTDK 463
++ +LLGYSG Y+G+Q N ++TI Y+ + + A++ R++RTDK
Sbjct: 40 RIIILLGYSGYGYHGIQINNPLKTIEGDVVAVLKKLGYLKTNNID-AEHLCIARAARTDK 98
Query: 464 GVSAAEQVVSLKL----PLEVNV--EEINKRLPETIKVFAVKRVTNRFNSKSKCNARSYS 625
GV ++SL L PL++++ E+N+ L I+V++V FN + C +R+Y
Sbjct: 99 GVHTLRNLISLNLFVDKPLDISLLKTELNEALCSQIRVWSVFPAPKYFNPRISCESRTYE 158
Query: 626 YTLPTY 643
Y +P++
Sbjct: 159 YLIPSF 164
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 63.3 bits (147), Expect = 3e-11
Identities = 39/125 (31%), Positives = 63/125 (50%), Gaps = 5/125 (4%)
Frame = +2
Query: 278 RKKMAM-LLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSR 454
RK+M ++ Y G + G+Q N V+TI + Q + ++ + + ++R
Sbjct: 35 RKRMVYCVVSYRGTGFAGLQYNANVKTIQDTLFQAFARVGAVVQVNADSPKKIRMCSAAR 94
Query: 455 TDKGVSAAEQVVSLKL----PLEVNVEEINKRLPETIKVFAVKRVTNRFNSKSKCNARSY 622
TDKGV A V+ LK+ PL V +N LP I+V+ + R N F+ + C++R Y
Sbjct: 95 TDKGVHAIVNVLGLKVLDNQPLPHVVNLVNDILPPCIRVWKMARTFNSFSPHTVCDSRVY 154
Query: 623 SYTLP 637
Y LP
Sbjct: 155 EYWLP 159
>SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 46.4 bits (105), Expect = 4e-06
Identities = 43/170 (25%), Positives = 72/170 (42%), Gaps = 9/170 (5%)
Frame = +2
Query: 146 KDEENKSIDIVPNKLLTRYNKRRAHRDWETKPPGEIKKPYERIKRKKMAMLLGYSGVEYY 325
K++ + +I P L + A +D + P + +P++ K +A Y G Y
Sbjct: 47 KNKLDNGNEIEPQHLSQELVLQHALQDASSLPKLKPVRPFQLEKTVFVAFKFAYCGWNYN 106
Query: 326 GM--QRNPG-VQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTDKGVSAAEQVVSL 496
G+ Q P + T+ IT + + F R RTDKGVSA QV+SL
Sbjct: 107 GLAYQLEPTPLPTVEGKVFEALLRAHLIT-----DPSSCSFSRCGRTDKGVSAMGQVISL 161
Query: 497 KL------PLEVNVEEINKRLPETIKVFAVKRVTNRFNSKSKCNARSYSY 628
+ P+ + + +N+ LP I++ F+++ C R Y Y
Sbjct: 162 NVRHSERRPI-IYCDVLNRLLPADIRITGYAFPPEGFDARFSCKQRHYKY 210
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 28.7 bits (61), Expect = 0.75
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -2
Query: 339 FRCIP*YSTPEYPSSIAIFFLLILSYGFLISP 244
++C Y E+P S+ +FF I+ + F SP
Sbjct: 1520 WKCYKAYDITEFPDSLKLFFSDIMGWNFFKSP 1551
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/42 (28%), Positives = 19/42 (45%)
Frame = +2
Query: 506 LEVNVEEINKRLPETIKVFAVKRVTNRFNSKSKCNARSYSYT 631
+E N+E++N P V A+K + NS +YT
Sbjct: 83 MESNIEKVNGLFPNDNSVIALKPNEEKLNSSLSVENNDSTYT 124
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 25.4 bits (53), Expect = 7.0
Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = -3
Query: 173 YRLTYFLHLSLRRQLPLRTASVRLLWLKYFFMNSHAFLV-CNY---PLVLYLPQLT 18
Y L FLH+ + L + AS+ + WL F ++ L+ NY P++ +P +T
Sbjct: 83 YFLALFLHVVEQPTLRIPMASLLMFWLFKFLASALVLLLRPNYTMFPMLNVVPSIT 138
>SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 283
Score = 25.0 bits (52), Expect = 9.3
Identities = 20/58 (34%), Positives = 26/58 (44%)
Frame = -2
Query: 291 AIFFLLILSYGFLISPGGLVSQSRCARRLLYLVNSLFGTISIDLFSSSFVTSAASITD 118
AIFFL SY I G+ C RR + L T DL S+ TS +I++
Sbjct: 11 AIFFLCSFSYVSSIKLCGIPGAPACKRREISLYKRATDTFP-DLSSAIASTSYYNISE 67
>SPCC132.01c ||SPCC1322.17c|DUF814 family
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1021
Score = 25.0 bits (52), Expect = 9.3
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = -2
Query: 279 LLILSYGFLISPGGLVSQSRCARRLLYLVNSLFGTISIDLFSSSFVTSAASITD 118
+LI+ YG L S+ R ARRL V G +S + VTS +I D
Sbjct: 686 VLIMGYGILWQLDEKSSERRKARRLEMEVVETQGKVSELKMEGTSVTSEDNIQD 739
>SPAC186.01 |||DIPSY family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 326
Score = 25.0 bits (52), Expect = 9.3
Identities = 14/48 (29%), Positives = 28/48 (58%)
Frame = -2
Query: 588 NLFVTLLTANTLIVSGNLLFISSTLTSKGSLSETTCSAADTPLSVLEL 445
N+ T +++ ++I S I++T+TS L TT + +TP+ +E+
Sbjct: 89 NIDETSVSSESIITST----ITTTITSGSQLYTTTITGQNTPVDTVEV 132
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,585,111
Number of Sequences: 5004
Number of extensions: 52022
Number of successful extensions: 178
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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