Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov10f08
(613 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g78660.3 68414.m09168 gamma-glutamyl hydrolase, putative / ga... 92 3e-19
At1g78660.2 68414.m09169 gamma-glutamyl hydrolase, putative / ga... 92 3e-19
At1g78660.1 68414.m09167 gamma-glutamyl hydrolase, putative / ga... 92 3e-19
At1g78680.1 68414.m09171 gamma-glutamyl hydrolase (GGH1) / gamma... 84 8e-17
At1g78670.1 68414.m09170 gamma-glutamyl hydrolase, putative / ga... 79 2e-15
At4g28670.1 68417.m04097 protein kinase family protein contains ... 32 0.34
At3g22720.1 68416.m02866 F-box family protein similar to F-box p... 29 3.2
At2g34890.1 68415.m04283 CTP synthase, putative / UTP--ammonia l... 29 3.2
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 28 5.6
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 28 5.6
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 28 5.6
At2g19940.2 68415.m02331 semialdehyde dehydrogenase family prote... 27 7.4
At2g19940.1 68415.m02330 semialdehyde dehydrogenase family prote... 27 7.4
At3g21170.1 68416.m02674 F-box family protein contains Pfam prof... 27 9.8
At2g41550.1 68415.m05135 expressed protein 27 9.8
>At1g78660.3 68414.m09168 gamma-glutamyl hydrolase, putative /
gamma-Glu-X carboxypeptidase, putative / conjugase,
putative similar to SP|O65355 Gamma-glutamyl hydrolase
precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase)
(Conjugase) (GH) {Arabidopsis thaliana}
Length = 348
Score = 91.9 bits (218), Expect = 3e-19
Identities = 45/121 (37%), Positives = 74/121 (61%)
Frame = +2
Query: 131 VNDRPIIGVLSQEQSFYLHGKFPEENYTSYIASSYVKDIEASGARVVPILIGKDRSYYKE 310
+N RP+IG+LS G+ + ++YIA+SYVK EA GARV+P++ + +
Sbjct: 51 LNYRPVIGILSHPGDG-ASGRLTNDTSSTYIAASYVKFAEAGGARVIPLIYNEPEEVLFQ 109
Query: 311 LMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFELLVILA 490
++ +NGV+ GG + + Y + + I+ A+E ND G++FP++G CLGFEL+ I+
Sbjct: 110 KLELVNGVIFTGG---WAKKYDYFEIVKKIFTKALERNDAGEHFPVYGICLGFELMSIII 166
Query: 491 S 493
S
Sbjct: 167 S 167
>At1g78660.2 68414.m09169 gamma-glutamyl hydrolase, putative /
gamma-Glu-X carboxypeptidase, putative / conjugase,
putative similar to SP|O65355 Gamma-glutamyl hydrolase
precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase)
(Conjugase) (GH) {Arabidopsis thaliana}
Length = 347
Score = 91.9 bits (218), Expect = 3e-19
Identities = 45/121 (37%), Positives = 74/121 (61%)
Frame = +2
Query: 131 VNDRPIIGVLSQEQSFYLHGKFPEENYTSYIASSYVKDIEASGARVVPILIGKDRSYYKE 310
+N RP+IG+LS G+ + ++YIA+SYVK EA GARV+P++ + +
Sbjct: 50 LNYRPVIGILSHPGDG-ASGRLTNDTSSTYIAASYVKFAEAGGARVIPLIYNEPEEVLFQ 108
Query: 311 LMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFELLVILA 490
++ +NGV+ GG + + Y + + I+ A+E ND G++FP++G CLGFEL+ I+
Sbjct: 109 KLELVNGVIFTGG---WAKKYDYFEIVKKIFTKALERNDAGEHFPVYGICLGFELMSIII 165
Query: 491 S 493
S
Sbjct: 166 S 166
>At1g78660.1 68414.m09167 gamma-glutamyl hydrolase, putative /
gamma-Glu-X carboxypeptidase, putative / conjugase,
putative similar to SP|O65355 Gamma-glutamyl hydrolase
precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase)
(Conjugase) (GH) {Arabidopsis thaliana}
Length = 348
Score = 91.9 bits (218), Expect = 3e-19
Identities = 45/121 (37%), Positives = 74/121 (61%)
Frame = +2
Query: 131 VNDRPIIGVLSQEQSFYLHGKFPEENYTSYIASSYVKDIEASGARVVPILIGKDRSYYKE 310
+N RP+IG+LS G+ + ++YIA+SYVK EA GARV+P++ + +
Sbjct: 51 LNYRPVIGILSHPGDG-ASGRLTNDTSSTYIAASYVKFAEAGGARVIPLIYNEPEEVLFQ 109
Query: 311 LMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFELLVILA 490
++ +NGV+ GG + + Y + + I+ A+E ND G++FP++G CLGFEL+ I+
Sbjct: 110 KLELVNGVIFTGG---WAKKYDYFEIVKKIFTKALERNDAGEHFPVYGICLGFELMSIII 166
Query: 491 S 493
S
Sbjct: 167 S 167
>At1g78680.1 68414.m09171 gamma-glutamyl hydrolase (GGH1) /
gamma-Glu-X carboxypeptidase / conjugase identical to
SP|O65355 Gamma-glutamyl hydrolase precursor (EC
3.4.19.9) (Gamma-Glu-X carboxypeptidase) (Conjugase)
(GH) {Arabidopsis thaliana}
Length = 347
Score = 83.8 bits (198), Expect = 8e-17
Identities = 43/121 (35%), Positives = 70/121 (57%)
Frame = +2
Query: 131 VNDRPIIGVLSQEQSFYLHGKFPEENYTSYIASSYVKDIEASGARVVPILIGKDRSYYKE 310
+N RP+IG+LS G+ S IA+SYVK E+ GARV+P++ + +
Sbjct: 50 LNYRPVIGILSHPGDG-ASGRLSNATDASSIAASYVKLAESGGARVIPLIFNEPEEILFQ 108
Query: 311 LMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFELLVILA 490
++ +NGV++ GG + + Y + + I+ +E ND G++FPI+ CLGFELL ++
Sbjct: 109 KLELVNGVILTGG---WAKEGLYFEIVKKIFNKVLERNDAGEHFPIYAICLGFELLTMII 165
Query: 491 S 493
S
Sbjct: 166 S 166
>At1g78670.1 68414.m09170 gamma-glutamyl hydrolase, putative /
gamma-Glu-X carboxypeptidase, putative / conjugase,
putative similar to gamma glutamyl hydrolase GI:1679658
SP|P93164 from [Glycine max]
Length = 352
Score = 79.4 bits (187), Expect = 2e-15
Identities = 42/127 (33%), Positives = 72/127 (56%), Gaps = 6/127 (4%)
Frame = +2
Query: 131 VNDRPIIGVLSQEQSFYLHGKFPE-ENYT-----SYIASSYVKDIEASGARVVPILIGKD 292
+N +P+IG+L+ + +NY SYIA+SYVK E GARV+P++ +
Sbjct: 54 LNYKPVIGILTHPGEGRWDARLHSLKNYAYATNISYIAASYVKLAETGGARVIPLIYNEP 113
Query: 293 RSYYKELMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFE 472
+ ++ +NGV+ GG + ++ Y D + I+ +E ND G++FP++ CLGFE
Sbjct: 114 EEILFQKLELVNGVIFTGG---WAKTGLYYDVVEKIFNKVMEKNDAGEHFPVYAMCLGFE 170
Query: 473 LLVILAS 493
+L ++ S
Sbjct: 171 ILSMIIS 177
>At4g28670.1 68417.m04097 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 625
Score = 31.9 bits (69), Expect = 0.34
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 497 RGKKENRITCHSFGNLPLDFTSDFRSSKMFS 589
+G+ N+I +SFG L L+ TS FR++K S
Sbjct: 510 KGRLSNKIDAYSFGVLVLEITSGFRNNKFRS 540
>At3g22720.1 68416.m02866 F-box family protein similar to F-box
protein family, AtFBX9 (GI:20197985) [Arabidopsis
thaliana]; contains Pfam PF00646: F-box domain; contains
TIGRFAM TIGR01640 : F-box protein interaction domain
Length = 378
Score = 28.7 bits (61), Expect = 3.2
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +2
Query: 80 VFHCEGAVIVDSNEYSVVNDRPIIGVLSQEQSFYLHGKFPEENYTSYIASSYVKDIEA 253
VFHC+G ++ +N+ +V P +G ++ ++ K Y S A Y+++ E+
Sbjct: 97 VFHCDGLLLCTTNDDRLVVWNPCLG-----ETKWIQLKVDYRRYVSIFALGYIQNNES 149
>At2g34890.1 68415.m04283 CTP synthase, putative / UTP--ammonia
ligase, putative similar to SP|P17812 CTP synthase (EC
6.3.4.2) (UTP--ammonia ligase) {Homo sapiens}; contains
Pfam profile PF00117: glutamine amidotransferase class-I
Length = 597
Score = 28.7 bits (61), Expect = 3.2
Identities = 20/59 (33%), Positives = 28/59 (47%)
Frame = +2
Query: 308 ELMKKINGVLIPGGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCLGFELLVI 484
+L+K NGVL+PG G+ D G LA + + P G CLG ++ VI
Sbjct: 358 KLLKDANGVLLPG---------GFGDRGVEGMILAAKYARENN-IPFLGICLGMQIAVI 406
>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 453
Score = 27.9 bits (59), Expect = 5.6
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +2
Query: 287 KDRSYYKELMKKINGVLIP-GGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCL 463
KD SY+K L K LI G TF +Q + Y + +H Y + VG +P +
Sbjct: 253 KDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH-YRAPEVILGVGWNYPCDLWSI 311
Query: 464 GFELLVILASG 496
G +LV L SG
Sbjct: 312 GC-ILVELCSG 321
>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 5.6
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +2
Query: 287 KDRSYYKELMKKINGVLIP-GGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCL 463
KD SY+K L K LI G TF +Q + Y + +H Y + VG +P +
Sbjct: 267 KDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH-YRAPEVILGVGWNYPCDLWSI 325
Query: 464 GFELLVILASG 496
G +LV L SG
Sbjct: 326 GC-ILVELCSG 335
>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 5.6
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +2
Query: 287 KDRSYYKELMKKINGVLIP-GGATFFNQSNGYADAGQHIYELAIELNDVGDYFPIFGTCL 463
KD SY+K L K LI G TF +Q + Y + +H Y + VG +P +
Sbjct: 267 KDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH-YRAPEVILGVGWNYPCDLWSI 325
Query: 464 GFELLVILASG 496
G +LV L SG
Sbjct: 326 GC-ILVELCSG 335
>At2g19940.2 68415.m02331 semialdehyde dehydrogenase family protein
similar to N-acetyl-glutamyl-phosphate reductase
[Campylobacter jejuni] GI:6650362; contains Pfam
profiles PF02774: Semialdehyde dehydrogenase
dimerisation domain, PF01118: Semialdehyde dehydrogenase
NAD binding domain
Length = 359
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -2
Query: 597 VLHENILLERKSEVKSSGRFPKE 529
+ HENI+++ KS V +GR KE
Sbjct: 181 IKHENIIIDAKSGVSGAGRGAKE 203
>At2g19940.1 68415.m02330 semialdehyde dehydrogenase family protein
similar to N-acetyl-glutamyl-phosphate reductase
[Campylobacter jejuni] GI:6650362; contains Pfam
profiles PF02774: Semialdehyde dehydrogenase
dimerisation domain, PF01118: Semialdehyde dehydrogenase
NAD binding domain
Length = 359
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -2
Query: 597 VLHENILLERKSEVKSSGRFPKE 529
+ HENI+++ KS V +GR KE
Sbjct: 181 IKHENIIIDAKSGVSGAGRGAKE 203
>At3g21170.1 68416.m02674 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 394
Score = 27.1 bits (57), Expect = 9.8
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +2
Query: 77 YVFHCEGAVIVDSNEYSVVNDRPIIG 154
+VFHC+G ++ + +Y +V P+ G
Sbjct: 106 HVFHCDGLLLCTTKDYRLVVWNPLSG 131
>At2g41550.1 68415.m05135 expressed protein
Length = 475
Score = 27.1 bits (57), Expect = 9.8
Identities = 15/25 (60%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = -1
Query: 475 ELKTKTGSEDRKIVSDV-VQFYSKL 404
ELK KTG E R I D+ VQ Y KL
Sbjct: 424 ELKKKTGKELRSIAKDLKVQHYYKL 448
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,366,898
Number of Sequences: 28952
Number of extensions: 283114
Number of successful extensions: 732
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 726
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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