BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10c03 (643 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 29 0.75 SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces... 28 1.00 SPBC1539.01c |||mitochondrial ribosomal protein subunit L15|Schi... 27 1.7 SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 27 3.0 SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 27 3.0 SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |S... 26 4.0 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 5.3 SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosacc... 25 7.0 SPBC887.10 |mcs4||two-component response regulator |Schizosaccha... 25 7.0 SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 25 9.3 SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccha... 25 9.3 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 28.7 bits (61), Expect = 0.75 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +3 Query: 9 STYLLLNYYCIDFFVYVSILANEIHAYENKYIYQSRLWNCIKA 137 + YL + CIDFFV V++ N ++A + SR + KA Sbjct: 1009 NAYLRSTWNCIDFFVLVTLWIN-LYAVLTSHALLSRAFRAFKA 1050 >SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 28.3 bits (60), Expect = 1.00 Identities = 17/55 (30%), Positives = 25/55 (45%) Frame = -3 Query: 266 NCLRPPELACLVVHLPPPASSFWSTNITVIHFNN*STAAVLQCSFDTIP*TTLID 102 N L P + +S+F S++ T I N T + L CSFD + + ID Sbjct: 571 NTLPPTSQGATSTTVSSASSNFLSSSCTPIDDTNSVTGSTLSCSFDEMKLSDKID 625 >SPBC1539.01c |||mitochondrial ribosomal protein subunit L15|Schizosaccharomyces pombe|chr 2|||Manual Length = 210 Score = 27.5 bits (58), Expect = 1.7 Identities = 7/15 (46%), Positives = 13/15 (86%) Frame = +3 Query: 90 ENKYIYQSRLWNCIK 134 ENK++++S WNC++ Sbjct: 14 ENKFVFRSSSWNCVR 28 >SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1462 Score = 26.6 bits (56), Expect = 3.0 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = +3 Query: 444 YNLDDMSYSLEEAENMDLRENI 509 Y++ D SYS+ E + ++REN+ Sbjct: 1096 YDIHDQSYSVHELHSENMRENV 1117 >SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 26.6 bits (56), Expect = 3.0 Identities = 23/68 (33%), Positives = 33/68 (48%) Frame = +3 Query: 3 INSTYLLLNYYCIDFFVYVSILANEIHAYENKYIYQSRLWNCIKAAL*NCCRGLIVKMNN 182 I+S L+N I +++ +EI ENK+I L N I++ L C G+I K Sbjct: 397 ISSILQLMNGLSISSYMFAITKMDEIEWDENKFI---NLVNSIQSFLKESC-GIIEKSKF 452 Query: 183 GDISAPKG 206 IS KG Sbjct: 453 IPISGLKG 460 >SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 26.2 bits (55), Expect = 4.0 Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 2/114 (1%) Frame = +3 Query: 60 SILANEIHAYENKYIYQSRLWNCIKAAL*NCCRGLIVKMNNGDISAPKGRGRGWQMNNQA 239 +I A+E YEN + SRL+ CR + +N G A G R +N Sbjct: 279 TIDAHESSLYEN--VNDSRLYEV------PACRNMA--LNTGYSDADPGYLRTSFRSNSH 328 Query: 240 RELRRPKAVSDEPKVDTSKSKLSADAK--EWYPANYTSQALPAYNTEPAPYRPS 395 L P + ++E V + +S + Y ++ ++P Y EP P RPS Sbjct: 329 NSL--PYSANEEEDVLQADFLVSQQSSMVSSYVSSRDPHSMPYYRREPIPLRPS 380 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 25.8 bits (54), Expect = 5.3 Identities = 13/49 (26%), Positives = 24/49 (48%) Frame = +3 Query: 318 KEWYPANYTSQALPAYNTEPAPYRPSRPSVQGRLRQAQDQNPYNLDDMS 464 K +PA+Y+S A N + + + S+ G ++ + + P NL S Sbjct: 422 KSIFPASYSSSAEDGQNMQAPAHAYMQSSIYGVNQEQKSEYPSNLSMQS 470 >SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 25.4 bits (53), Expect = 7.0 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +3 Query: 453 DDMSYSLEEAENMDLRENIANLITVMCE 536 DD S+ + E+ +RE + I V+CE Sbjct: 7 DDFSFEKRKTESQRIREKYPDRIPVICE 34 >SPBC887.10 |mcs4||two-component response regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 25.4 bits (53), Expect = 7.0 Identities = 21/91 (23%), Positives = 39/91 (42%) Frame = +3 Query: 222 QMNNQARELRRPKAVSDEPKVDTSKSKLSADAKEWYPANYTSQALPAYNTEPAPYRPSRP 401 Q +++A + R ++ + D S ++ A A+ P N S + + T+ + P Sbjct: 225 QPSDEAESITRKNSIGMSTRSDESTAEKLAKAEVATPTNSRSISHSSLYTKQSGTAGVLP 284 Query: 402 SVQGRLRQAQDQNPYNLDDMSYSLEEAENMD 494 +V + A NP D+S A+N D Sbjct: 285 AVNADIDAANRMNP----DISSQFPIADNKD 311 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 25.0 bits (52), Expect = 9.3 Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 1/55 (1%) Frame = +3 Query: 285 DTSKSKLSADAKEWYP-ANYTSQALPAYNTEPAPYRPSRPSVQGRLRQAQDQNPY 446 + +K+K A+ P A S + + +P+ Y +R LRQ +D NPY Sbjct: 41 ELAKAKKQAEKAASAPVAAPKSSSKKEEDLDPSQYFENRSRTIMELRQTKDPNPY 95 >SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 25.0 bits (52), Expect = 9.3 Identities = 11/45 (24%), Positives = 22/45 (48%) Frame = +3 Query: 249 RRPKAVSDEPKVDTSKSKLSADAKEWYPANYTSQALPAYNTEPAP 383 RR ++ SDE + S +S ++ + + + + P+ T P P Sbjct: 320 RRSRSYSDEDEESVYSSDVSTESSSQFSSRSSEYSKPSRPTAPKP 364 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,791,987 Number of Sequences: 5004 Number of extensions: 60190 Number of successful extensions: 193 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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