BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10c01 (717 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosacc... 156 3e-39 SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 77 2e-15 SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo... 76 4e-15 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 49 6e-07 SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 29 0.88 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 28 1.5 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 27 2.7 SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schi... 27 3.5 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 27 3.5 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 26 4.7 SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schiz... 26 6.2 SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pomb... 25 8.2 SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||... 25 8.2 SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 8.2 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 25 8.2 SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 25 8.2 >SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 156 bits (379), Expect = 3e-39 Identities = 78/173 (45%), Positives = 111/173 (64%), Gaps = 7/173 (4%) Frame = +1 Query: 82 MNYENKIILAPMVRIGTLPMRLLALKYGADIVYTEELIDWKFLRS---KRRFNDILNTVD 252 +NY NK+ LAPMVRIG LPMRLLAL+YGA++V+ E++D L +R ND +N +D Sbjct: 4 LNYSNKVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCID 63 Query: 253 YVDQTDGTIVFRTCEEEKKKVVLQLGTCDEARALKVAKLVEHDVAAIDINMGCPKEFSIK 432 +V ++FR E +++ QLG+ A++ AKLV +DVA ID+N GCPK FS+ Sbjct: 64 FVKPPSNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVH 123 Query: 433 GGMGVALLSKPDKAYQILKTLVDNLSIP----VTCKIRILKTPEATLELVNKL 579 GMG LL D+ IL LV+ + P ++CKIR+L+T E TL+LV ++ Sbjct: 124 AGMGAGLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERI 176 >SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 326 Score = 77.4 bits (182), Expect = 2e-15 Identities = 58/169 (34%), Positives = 81/169 (47%), Gaps = 2/169 (1%) Frame = +1 Query: 70 FPINMNYENKIILAPMVRIGTLPMRLLALKYGADIVYTEELIDWKFLRSKRRFNDILNTV 249 F IN I APMVR LP R L Y DIVYT ++ +FL K R+ D Sbjct: 12 FEINKGKRPVHIAAPMVRYSKLPFRQLVRDYNTDIVYTPMILAKEFLHPKGRYFD----- 66 Query: 250 DYVDQTDGTIVFRTCEEEKKKVVLQLGTCDEARALKVAKLVEHDVAAIDINMGCPKEFSI 429 F T + + ++LQ G D K A+LV V I IN GCP+ ++I Sbjct: 67 -----------FSTNDADAS-LILQFGVDDPVILEKAAQLVGPYVDGIGINCGCPQTWAI 114 Query: 430 KGGMGVALLSKPDKAYQILKTLVDNL--SIPVTCKIRILKTPEATLELV 570 + G+G ALL +P+K +++++ + L S KIRI K T L+ Sbjct: 115 QEGIGSALLDEPEKVHKLVRAVKSTLGESFCTEVKIRIAKDLNKTRHLM 163 >SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 76.2 bits (179), Expect = 4e-15 Identities = 55/162 (33%), Positives = 77/162 (47%) Frame = +1 Query: 97 KIILAPMVRIGTLPMRLLALKYGADIVYTEELIDWKFLRSKRRFNDILNTVDYVDQTDGT 276 K ILAPMV LP R+LA + GAD+ Y+ F S+ N + +T Sbjct: 19 KRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFST---------- 68 Query: 277 IVFRTCEEEKKKVVLQLGTCDEARALKVAKLVEHDVAAIDINMGCPKEFSIKGGMGVALL 456 RT EE+ ++ G D LK AK+ A+D+N+GCP+ + KG G L Sbjct: 69 ---RTIPEERPLIIQFCGN-DPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQ 124 Query: 457 SKPDKAYQILKTLVDNLSIPVTCKIRILKTPEATLELVNKLI 582 + I+ L LSIPVT KIRI P+ TL+ ++ Sbjct: 125 ENWNLIESIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMIL 166 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 49.2 bits (112), Expect = 6e-07 Identities = 41/157 (26%), Positives = 79/157 (50%), Gaps = 5/157 (3%) Frame = +1 Query: 82 MNYENKIILAPMVRIGTLPMRLLALKYGADIVYTEELIDWKFLRSKRRFNDILNTVDYVD 261 +++ ++ ILAP+ +G P R L GAD Y+E + + ++ + ++ ++Y Sbjct: 235 IDWRDRKILAPLTTVGNPPFRRLCGSLGADTFYSEMAMCYPLMQGHQPEWALVRGLNYER 294 Query: 262 QTDGTIVFRTCEEEKKKVVLQLGTCDEARALKVAKLVEHD---VAAIDINMGCPKEFSIK 432 + + R + + +QL T +A K A+++ V +D+N GCP + + Sbjct: 295 E-----MMR--GGRRGILGVQLATGKLWQATKTAQVIAEQCDGVDFLDLNCGCPIDLVFR 347 Query: 433 GGMGVALLSKPDKAYQILKTLVDNLS--IPVTCKIRI 537 G G +LL P + + L+ + D +S IPVT K+R+ Sbjct: 348 QGAGSSLLENPGRLLRNLQGM-DAVSGQIPVTVKLRM 383 >SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1389 Score = 28.7 bits (61), Expect = 0.88 Identities = 12/15 (80%), Positives = 12/15 (80%) Frame = -3 Query: 118 PLAQVLFYFRNSCLW 74 PLAQ LF FRNS LW Sbjct: 301 PLAQALFAFRNSDLW 315 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/42 (28%), Positives = 25/42 (59%) Frame = +1 Query: 178 YTEELIDWKFLRSKRRFNDILNTVDYVDQTDGTIVFRTCEEE 303 YT+E I+W F+ ND+ T+D +++++ +F +E+ Sbjct: 498 YTQERIEWDFIDYG---NDLQPTIDAIEKSEPIGIFSCLDED 536 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.1 bits (57), Expect = 2.7 Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 5/67 (7%) Frame = -2 Query: 455 NSATPMPPFIENSFGHPILISIAATSC-----STNLATFKALASSHVPSCKTTFFFSSSQ 291 +S++ +P I S P+ +S ++ S STNL T + ++ + ++ F+S+S Sbjct: 240 SSSSVLPTSIITSTSTPVTVSSSSLSSFTPSYSTNLTTTGSTTTTGSATVSSSPFYSNSS 299 Query: 290 VRNTIVP 270 V T VP Sbjct: 300 VIPTSVP 306 >SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schizosaccharomyces pombe|chr 2|||Manual Length = 1107 Score = 26.6 bits (56), Expect = 3.5 Identities = 17/56 (30%), Positives = 26/56 (46%) Frame = +1 Query: 7 IHYLIRRCRFKTFIT*SILLQFPINMNYENKIILAPMVRIGTLPMRLLALKYGADI 174 +HYLIRRC T I I F N N + +++ + + +P L YG + Sbjct: 851 VHYLIRRCEALTNIINRI--SFRNNPNMDGEVLNELNISLLEIPNLRLQAFYGITV 904 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +1 Query: 193 IDWKFLRSKRRFNDILNTVDYVDQTDGTIVF 285 + + F+ +K ND+ N YVD +D I+F Sbjct: 191 LPFSFMPAKANVNDLFNISAYVDTSDLPILF 221 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 26.2 bits (55), Expect = 4.7 Identities = 16/43 (37%), Positives = 22/43 (51%) Frame = -2 Query: 461 FDNSATPMPPFIENSFGHPILISIAATSCSTNLATFKALASSH 333 F +SAT F S G PI S+ +TS N A+ +SS+ Sbjct: 299 FPSSATNSFSFEHGSAGFPIPGSVPSTSYHANTASEDGFSSSY 341 >SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 547 Score = 25.8 bits (54), Expect = 6.2 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = -2 Query: 467 SGFDNSATPMPPFIENSFGHPILISIAATSCSTNLATFKALASSHVPSCKTTFFFS 300 SGF +S +P P S+A+++ STNL K L + S +FS Sbjct: 207 SGFPSSLNGIPIASPPLATSPTSFSLASSASSTNLGGSKGLLFQQMTSENNRDYFS 262 >SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 323 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +1 Query: 193 IDWKFLRSKRRFNDILNTVDYVDQTDGTIVFRTC 294 ID +F RRF+ + +D +DQ TI R+C Sbjct: 193 IDQRFNSIDRRFDSMEQRLDSMDQKMETIDARSC 226 >SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||Manual Length = 247 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +1 Query: 193 IDWKFLRSKRRFNDILNTVDYVDQTDGTIVFRTC 294 ID +F RRF+ + +D +DQ TI R+C Sbjct: 193 IDQRFNSIDRRFDSMEQRLDSMDQKMETIDARSC 226 >SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1877 Score = 25.4 bits (53), Expect = 8.2 Identities = 8/19 (42%), Positives = 12/19 (63%) Frame = -1 Query: 447 HTHATFYRKFFWASHINIY 391 HT T + K F+ASH+ + Sbjct: 164 HTKTTIFEKVFYASHLGFF 182 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 25.4 bits (53), Expect = 8.2 Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = +1 Query: 211 RSKRRFNDILNTVDYVDQTDGTIVFRTCEE-EKKKVVLQ 324 R K RF +LN + VD T + + CE EK+K L+ Sbjct: 1334 RWKLRFQSVLNKYERVDPTQLEELKKNCEALEKEKQELE 1372 >SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 25.4 bits (53), Expect = 8.2 Identities = 14/49 (28%), Positives = 21/49 (42%) Frame = +1 Query: 268 DGTIVFRTCEEEKKKVVLQLGTCDEARALKVAKLVEHDVAAIDINMGCP 414 DG F+T EK + + C A ++ + + D A I MG P Sbjct: 51 DGREYFKTKNNEKTGIFVPFDKCKLASSISSSPSPKIDGTAASIGMGFP 99 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,952,676 Number of Sequences: 5004 Number of extensions: 59961 Number of successful extensions: 161 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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