BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov10a15 (676 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 79 2e-15 At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 79 2e-15 At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 43 2e-04 At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 43 2e-04 At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 42 4e-04 At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 39 0.004 At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 35 0.057 At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 35 0.057 At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 35 0.057 At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 34 0.075 At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 34 0.100 At2g39480.1 68415.m04845 ABC transporter family protein related ... 32 0.40 At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 31 0.93 At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 29 2.8 At1g06730.1 68414.m00715 pfkB-type carbohydrate kinase family pr... 29 3.8 At5g20420.1 68418.m02428 SNF2 domain-containing protein / helica... 28 6.6 At1g12930.1 68414.m01501 importin-related similar to late gestat... 28 6.6 At1g08700.1 68414.m00966 presenilin family protein similar to SP... 28 6.6 >At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrogenase E2 subunit (din3) identical to branched chain alpha-keto acid dehydrogenase E2 subunit (din3) [Arabidopsis thaliana] GI:7021284 Length = 483 Score = 79.4 bits (187), Expect = 2e-15 Identities = 37/88 (42%), Positives = 55/88 (62%) Frame = +2 Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565 ++ L+ GEGI E + +WFVK GD+V++F +CEVQSDKA + ITSR+ G + + H Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134 Query: 566 DIDQTALVGQPLVDIDVQDSENDGKPTD 649 VG+ LV + V+DS++ TD Sbjct: 135 SPGDIIKVGETLVRLAVEDSQDSLLTTD 162 >At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrogenase E2 subunit (din3) identical to branched chain alpha-keto acid dehydrogenase E2 subunit (din3) [Arabidopsis thaliana] GI:7021284 Length = 483 Score = 79.4 bits (187), Expect = 2e-15 Identities = 37/88 (42%), Positives = 55/88 (62%) Frame = +2 Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565 ++ L+ GEGI E + +WFVK GD+V++F +CEVQSDKA + ITSR+ G + + H Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134 Query: 566 DIDQTALVGQPLVDIDVQDSENDGKPTD 649 VG+ LV + V+DS++ TD Sbjct: 135 SPGDIIKVGETLVRLAVEDSQDSLLTTD 162 >At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase [Zea mays] GI:5669871; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain Length = 539 Score = 43.2 bits (97), Expect = 2e-04 Identities = 29/131 (22%), Positives = 58/131 (44%), Gaps = 3/131 (2%) Frame = +2 Query: 275 VTTQSARNGSQLSYKTPLNESLSKELRH---FHTSHAVNKIVAFKLSDIGEGIREVVIKE 445 ++T S + S ++ E +S ++R F +S + + + + E I Sbjct: 71 ISTTSTKLSSPMAGPKLFKEFISSQMRSVRGFSSSSDLPPHQEIGMPSLSPTMTEGNIAR 130 Query: 446 WFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPLVDIDVQDS 625 W K GD V + +CEV++DKA V + +G + ++ + + ++ I V+D Sbjct: 131 WLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEIQVGEVIAITVEDE 190 Query: 626 ENDGKPTDVAP 658 ++ K D P Sbjct: 191 DDIQKFKDYTP 201 >At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase GI:5669871 [Zea mays]; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain Length = 539 Score = 43.2 bits (97), Expect = 2e-04 Identities = 22/74 (29%), Positives = 36/74 (48%) Frame = +2 Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPLVDIDV 616 I W K GD V + +CEV++DKA V + +G + ++ + ++ I V Sbjct: 128 IARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEGSKEIQVGEVIAITV 187 Query: 617 QDSENDGKPTDVAP 658 +D E+ GK D P Sbjct: 188 EDEEDIGKFKDYTP 201 >At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein similar to SP|Q01205 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC 2.3.1.61) {Rattus norvegicus}; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme Length = 464 Score = 41.9 bits (94), Expect = 4e-04 Identities = 24/95 (25%), Positives = 44/95 (46%), Gaps = 1/95 (1%) Frame = +2 Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565 +V + +GE I + + + K GD V+ + I ++++DK + I S G+I Sbjct: 93 VVEAVVPHMGESITDGTLAAFLKKPGDRVEADEAIAQIETDKVTIDIASPASGVIQEFLV 152 Query: 566 DIDQTALVGQPLVDIDVQ-DSENDGKPTDVAPDKP 667 T G + I D+ + P++ AP+KP Sbjct: 153 KEGDTVEPGNKVARISTSADAVSHVAPSEKAPEKP 187 >At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide acetyltransferase (E2) subunit of PDC [Arabidopsis thaliana] GI:559395; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain; supporting cDNA gi|5881964|gb|AF066080.1|AF066080 Length = 637 Score = 38.7 bits (86), Expect = 0.004 Identities = 14/41 (34%), Positives = 26/41 (63%) Frame = +2 Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRL 559 + +W K GD V+ D +CE+++DKA V S+ +G + ++ Sbjct: 102 VVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKI 142 Score = 37.9 bits (84), Expect = 0.006 Identities = 21/66 (31%), Positives = 39/66 (59%), Gaps = 1/66 (1%) Frame = +2 Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITR-LYHDIDQTALVGQPLVDID 613 I +W+ K GD ++ D I E+++DKA + S +G + + L + + VG+P+ I Sbjct: 229 IAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYLAKILIPEGSKDVAVGKPIALI- 287 Query: 614 VQDSEN 631 V+D+E+ Sbjct: 288 VEDAES 293 >At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein similar to SP|P36957 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC 2.3.1.61) {Homo sapiens}; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme Length = 365 Score = 34.7 bits (76), Expect = 0.057 Identities = 21/89 (23%), Positives = 41/89 (46%) Frame = +2 Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589 +GE I + + + K G+ VQ + I ++++DK + I S G+I + T Sbjct: 1 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 60 Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676 G + I S+++ + V P + + E Sbjct: 61 GTKVAII----SKSEDTASQVTPSQKIPE 85 >At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein similar to SP|P36957 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC 2.3.1.61) {Homo sapiens}; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme Length = 463 Score = 34.7 bits (76), Expect = 0.057 Identities = 21/89 (23%), Positives = 41/89 (46%) Frame = +2 Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589 +GE I + + + K G+ VQ + I ++++DK + I S G+I + T Sbjct: 99 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 158 Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676 G + I S+++ + V P + + E Sbjct: 159 GTKVAII----SKSEDTASQVTPSQKIPE 183 >At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein similar to SP|P36957 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC 2.3.1.61) {Homo sapiens}; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme Length = 464 Score = 34.7 bits (76), Expect = 0.057 Identities = 21/89 (23%), Positives = 41/89 (46%) Frame = +2 Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589 +GE I + + + K G+ VQ + I ++++DK + I S G+I + T Sbjct: 100 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 159 Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676 G + I S+++ + V P + + E Sbjct: 160 GTKVAII----SKSEDTASQVTPSQKIPE 184 >At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (LTA2) identical to dihydrolipoamide S-acetyltransferase (LTA2) [Arabidopsis thaliana] GI:5881963 Length = 480 Score = 34.3 bits (75), Expect = 0.075 Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 5/109 (4%) Frame = +2 Query: 281 TQSARNGSQLSYKTPLNESLSKELRHFHTSHAVNKIVAFKLSDI-----GEGIREVVIKE 445 T S N S S +P S+ TSH + V K+ +I + E I Sbjct: 15 TNSKSNISFASSVSPSLRSVVFRSTTPATSHRRSMTVRSKIREIFMPALSSTMTEGKIVS 74 Query: 446 WFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVG 592 W G+ + + +++ V+SDKA + + + YDG + + +TA VG Sbjct: 75 WIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEGETAPVG 123 >At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase (LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain Length = 465 Score = 33.9 bits (74), Expect = 0.100 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 1/87 (1%) Frame = +2 Query: 383 KIVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLY 562 KI + + + E I W GD + + +++ V+SDKA + + + YDG + + Sbjct: 38 KIREIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIM 97 Query: 563 HDIDQTALVGQPLVDI-DVQDSENDGK 640 + A VG + + + +D D K Sbjct: 98 VEEGGVAPVGSAIALLAETEDEIADAK 124 >At2g39480.1 68415.m04845 ABC transporter family protein related to multi drug resistance proteins and P-glycoproteins Length = 1407 Score = 31.9 bits (69), Expect = 0.40 Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = -1 Query: 550 YDTVVSASDGDSCLVAL-YFANVVELLNIITDFDEPFLDDDFS 425 + +V +A+ G + +V L YFA +V++L TD D DD F+ Sbjct: 88 FGSVAAAAHGTALIVYLHYFAKIVQVLAFPTDSDHLISDDQFN 130 >At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2) identical to biotin carboxyl carrier protein isoform 2 [Arabidopsis thaliana] gi|8886869|gb|AAF80592 Length = 255 Score = 30.7 bits (66), Expect = 0.93 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +2 Query: 449 FVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPL 601 FVKVGD VQ+ +C +++ K I + G I L + + V PL Sbjct: 200 FVKVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVDTPL 250 >At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1) identical to biotin carboxyl carrier protein of acetyl-CoA carboxylase precursor [Arabidopsis thaliana] gi|9759121|dbj|BAB09606 Length = 280 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/51 (29%), Positives = 26/51 (50%) Frame = +2 Query: 449 FVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPL 601 F+KVGD VQ+ +C V++ K I S + G + + + + + PL Sbjct: 225 FIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPL 275 >At1g06730.1 68414.m00715 pfkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 pfkB family carbohydrate kinase Length = 488 Score = 28.7 bits (61), Expect = 3.8 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = -2 Query: 243 SWNTDLRTRIDIAMKELRTRILNNYKEHDASPVF 142 SW TDL + +A+++ + N Y D SP F Sbjct: 220 SWITDLSDEVKMAIRQSKVLFCNGYDFDDFSPSF 253 >At5g20420.1 68418.m02428 SNF2 domain-containing protein / helicase domain-containing protein low similarity to SP|Q9U7E0 Transcriptional regulator ATRX homolog {Caenorhabditis elegans}; contains PFam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain Length = 1261 Score = 27.9 bits (59), Expect = 6.6 Identities = 30/122 (24%), Positives = 57/122 (46%) Frame = +2 Query: 146 TGLASCSL*LFKIRVLSSFIAMSILVRRSVFQLRTVNRCRKIKVTTQSARNGSQLSYKTP 325 T L L L K S++ ++ ++ VFQ+RTV ++ T + S LS Sbjct: 172 TSLMKTRLSLGKFLPDLSWLTVTSTLKSIVFQIRTVQTKMVYQIVTDEEGSSSTLS---S 228 Query: 326 LNESLSKELRHFHTSHAVNKIVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQS 505 +N +L + +++K+V F +DI + +++ IK+ D Q+ D + E++ Sbjct: 229 MNITLEDGV-------SLSKVVKFNPADILDDSQDLEIKQ----ETDYYQEEDEVVELRR 277 Query: 506 DK 511 K Sbjct: 278 SK 279 >At1g12930.1 68414.m01501 importin-related similar to late gestation lung 2 protein (GI:7274209) {Rattus norvegicus}; similar to Ran binding protein 13 (importin 13)) (GI:8133102) {Homo sapiens}; contains weak hit to Pfam PF03810: Importin-beta N-terminal domain Length = 1005 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = -1 Query: 595 LSYKSGLINIMVQSCYDTVVSASDGDSCLVAL 500 +SY SG + + + S +TV S SDG +V++ Sbjct: 831 MSYLSGFLEVSLSSMIETVNSISDGSFSVVSV 862 >At1g08700.1 68414.m00966 presenilin family protein similar to SP|P52166 Presenilin sel-12 {Caenorhabditis elegans}; contains Pfam profile PF01080: Presenilin Length = 453 Score = 27.9 bits (59), Expect = 6.6 Identities = 14/27 (51%), Positives = 16/27 (59%) Frame = -2 Query: 675 SATGLSGATSVGFPSFSESCTSISTRG 595 S GL + VG PS SE TS+ TRG Sbjct: 296 SRGGLERSPLVGSPSASEHSTSVGTRG 322 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,340,406 Number of Sequences: 28952 Number of extensions: 260462 Number of successful extensions: 767 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 743 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 767 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1432596384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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