BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov10a15
(676 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 79 2e-15
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 79 2e-15
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 43 2e-04
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 43 2e-04
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 42 4e-04
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 39 0.004
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 35 0.057
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 35 0.057
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 35 0.057
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 34 0.075
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 34 0.100
At2g39480.1 68415.m04845 ABC transporter family protein related ... 32 0.40
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 31 0.93
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 29 2.8
At1g06730.1 68414.m00715 pfkB-type carbohydrate kinase family pr... 29 3.8
At5g20420.1 68418.m02428 SNF2 domain-containing protein / helica... 28 6.6
At1g12930.1 68414.m01501 importin-related similar to late gestat... 28 6.6
At1g08700.1 68414.m00966 presenilin family protein similar to SP... 28 6.6
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 79.4 bits (187), Expect = 2e-15
Identities = 37/88 (42%), Positives = 55/88 (62%)
Frame = +2
Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565
++ L+ GEGI E + +WFVK GD+V++F +CEVQSDKA + ITSR+ G + + H
Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134
Query: 566 DIDQTALVGQPLVDIDVQDSENDGKPTD 649
VG+ LV + V+DS++ TD
Sbjct: 135 SPGDIIKVGETLVRLAVEDSQDSLLTTD 162
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 79.4 bits (187), Expect = 2e-15
Identities = 37/88 (42%), Positives = 55/88 (62%)
Frame = +2
Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565
++ L+ GEGI E + +WFVK GD+V++F +CEVQSDKA + ITSR+ G + + H
Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134
Query: 566 DIDQTALVGQPLVDIDVQDSENDGKPTD 649
VG+ LV + V+DS++ TD
Sbjct: 135 SPGDIIKVGETLVRLAVEDSQDSLLTTD 162
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 43.2 bits (97), Expect = 2e-04
Identities = 29/131 (22%), Positives = 58/131 (44%), Gaps = 3/131 (2%)
Frame = +2
Query: 275 VTTQSARNGSQLSYKTPLNESLSKELRH---FHTSHAVNKIVAFKLSDIGEGIREVVIKE 445
++T S + S ++ E +S ++R F +S + + + + E I
Sbjct: 71 ISTTSTKLSSPMAGPKLFKEFISSQMRSVRGFSSSSDLPPHQEIGMPSLSPTMTEGNIAR 130
Query: 446 WFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPLVDIDVQDS 625
W K GD V + +CEV++DKA V + +G + ++ + + ++ I V+D
Sbjct: 131 WLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEIQVGEVIAITVEDE 190
Query: 626 ENDGKPTDVAP 658
++ K D P
Sbjct: 191 DDIQKFKDYTP 201
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 43.2 bits (97), Expect = 2e-04
Identities = 22/74 (29%), Positives = 36/74 (48%)
Frame = +2
Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPLVDIDV 616
I W K GD V + +CEV++DKA V + +G + ++ + ++ I V
Sbjct: 128 IARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEGSKEIQVGEVIAITV 187
Query: 617 QDSENDGKPTDVAP 658
+D E+ GK D P
Sbjct: 188 EDEEDIGKFKDYTP 201
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 41.9 bits (94), Expect = 4e-04
Identities = 24/95 (25%), Positives = 44/95 (46%), Gaps = 1/95 (1%)
Frame = +2
Query: 386 IVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYH 565
+V + +GE I + + + K GD V+ + I ++++DK + I S G+I
Sbjct: 93 VVEAVVPHMGESITDGTLAAFLKKPGDRVEADEAIAQIETDKVTIDIASPASGVIQEFLV 152
Query: 566 DIDQTALVGQPLVDIDVQ-DSENDGKPTDVAPDKP 667
T G + I D+ + P++ AP+KP
Sbjct: 153 KEGDTVEPGNKVARISTSADAVSHVAPSEKAPEKP 187
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 38.7 bits (86), Expect = 0.004
Identities = 14/41 (34%), Positives = 26/41 (63%)
Frame = +2
Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRL 559
+ +W K GD V+ D +CE+++DKA V S+ +G + ++
Sbjct: 102 VVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKI 142
Score = 37.9 bits (84), Expect = 0.006
Identities = 21/66 (31%), Positives = 39/66 (59%), Gaps = 1/66 (1%)
Frame = +2
Query: 437 IKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITR-LYHDIDQTALVGQPLVDID 613
I +W+ K GD ++ D I E+++DKA + S +G + + L + + VG+P+ I
Sbjct: 229 IAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYLAKILIPEGSKDVAVGKPIALI- 287
Query: 614 VQDSEN 631
V+D+E+
Sbjct: 288 VEDAES 293
>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 365
Score = 34.7 bits (76), Expect = 0.057
Identities = 21/89 (23%), Positives = 41/89 (46%)
Frame = +2
Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589
+GE I + + + K G+ VQ + I ++++DK + I S G+I + T
Sbjct: 1 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 60
Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676
G + I S+++ + V P + + E
Sbjct: 61 GTKVAII----SKSEDTASQVTPSQKIPE 85
>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 463
Score = 34.7 bits (76), Expect = 0.057
Identities = 21/89 (23%), Positives = 41/89 (46%)
Frame = +2
Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589
+GE I + + + K G+ VQ + I ++++DK + I S G+I + T
Sbjct: 99 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 158
Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676
G + I S+++ + V P + + E
Sbjct: 159 GTKVAII----SKSEDTASQVTPSQKIPE 183
>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 34.7 bits (76), Expect = 0.057
Identities = 21/89 (23%), Positives = 41/89 (46%)
Frame = +2
Query: 410 IGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALV 589
+GE I + + + K G+ VQ + I ++++DK + I S G+I + T
Sbjct: 100 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEP 159
Query: 590 GQPLVDIDVQDSENDGKPTDVAPDKPVAE 676
G + I S+++ + V P + + E
Sbjct: 160 GTKVAII----SKSEDTASQVTPSQKIPE 184
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 34.3 bits (75), Expect = 0.075
Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 5/109 (4%)
Frame = +2
Query: 281 TQSARNGSQLSYKTPLNESLSKELRHFHTSHAVNKIVAFKLSDI-----GEGIREVVIKE 445
T S N S S +P S+ TSH + V K+ +I + E I
Sbjct: 15 TNSKSNISFASSVSPSLRSVVFRSTTPATSHRRSMTVRSKIREIFMPALSSTMTEGKIVS 74
Query: 446 WFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVG 592
W G+ + + +++ V+SDKA + + + YDG + + +TA VG
Sbjct: 75 WIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEGETAPVG 123
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 33.9 bits (74), Expect = 0.100
Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +2
Query: 383 KIVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLY 562
KI + + + E I W GD + + +++ V+SDKA + + + YDG + +
Sbjct: 38 KIREIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIM 97
Query: 563 HDIDQTALVGQPLVDI-DVQDSENDGK 640
+ A VG + + + +D D K
Sbjct: 98 VEEGGVAPVGSAIALLAETEDEIADAK 124
>At2g39480.1 68415.m04845 ABC transporter family protein related to
multi drug resistance proteins and P-glycoproteins
Length = 1407
Score = 31.9 bits (69), Expect = 0.40
Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = -1
Query: 550 YDTVVSASDGDSCLVAL-YFANVVELLNIITDFDEPFLDDDFS 425
+ +V +A+ G + +V L YFA +V++L TD D DD F+
Sbjct: 88 FGSVAAAAHGTALIVYLHYFAKIVQVLAFPTDSDHLISDDQFN 130
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 30.7 bits (66), Expect = 0.93
Identities = 17/51 (33%), Positives = 25/51 (49%)
Frame = +2
Query: 449 FVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPL 601
FVKVGD VQ+ +C +++ K I + G I L + + V PL
Sbjct: 200 FVKVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVDTPL 250
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = +2
Query: 449 FVKVGDNVQQFDNICEVQSDKAAVTITSRYDGIITRLYHDIDQTALVGQPL 601
F+KVGD VQ+ +C V++ K I S + G + + + + + PL
Sbjct: 225 FIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPL 275
>At1g06730.1 68414.m00715 pfkB-type carbohydrate kinase family
protein contains Pfam profile: PF00294 pfkB family
carbohydrate kinase
Length = 488
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -2
Query: 243 SWNTDLRTRIDIAMKELRTRILNNYKEHDASPVF 142
SW TDL + +A+++ + N Y D SP F
Sbjct: 220 SWITDLSDEVKMAIRQSKVLFCNGYDFDDFSPSF 253
>At5g20420.1 68418.m02428 SNF2 domain-containing protein / helicase
domain-containing protein low similarity to SP|Q9U7E0
Transcriptional regulator ATRX homolog {Caenorhabditis
elegans}; contains PFam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain
Length = 1261
Score = 27.9 bits (59), Expect = 6.6
Identities = 30/122 (24%), Positives = 57/122 (46%)
Frame = +2
Query: 146 TGLASCSL*LFKIRVLSSFIAMSILVRRSVFQLRTVNRCRKIKVTTQSARNGSQLSYKTP 325
T L L L K S++ ++ ++ VFQ+RTV ++ T + S LS
Sbjct: 172 TSLMKTRLSLGKFLPDLSWLTVTSTLKSIVFQIRTVQTKMVYQIVTDEEGSSSTLS---S 228
Query: 326 LNESLSKELRHFHTSHAVNKIVAFKLSDIGEGIREVVIKEWFVKVGDNVQQFDNICEVQS 505
+N +L + +++K+V F +DI + +++ IK+ D Q+ D + E++
Sbjct: 229 MNITLEDGV-------SLSKVVKFNPADILDDSQDLEIKQ----ETDYYQEEDEVVELRR 277
Query: 506 DK 511
K
Sbjct: 278 SK 279
>At1g12930.1 68414.m01501 importin-related similar to late gestation
lung 2 protein (GI:7274209) {Rattus norvegicus}; similar
to Ran binding protein 13 (importin 13)) (GI:8133102)
{Homo sapiens}; contains weak hit to Pfam PF03810:
Importin-beta N-terminal domain
Length = 1005
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 595 LSYKSGLINIMVQSCYDTVVSASDGDSCLVAL 500
+SY SG + + + S +TV S SDG +V++
Sbjct: 831 MSYLSGFLEVSLSSMIETVNSISDGSFSVVSV 862
>At1g08700.1 68414.m00966 presenilin family protein similar to
SP|P52166 Presenilin sel-12 {Caenorhabditis elegans};
contains Pfam profile PF01080: Presenilin
Length = 453
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = -2
Query: 675 SATGLSGATSVGFPSFSESCTSISTRG 595
S GL + VG PS SE TS+ TRG
Sbjct: 296 SRGGLERSPLVGSPSASEHSTSVGTRG 322
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,340,406
Number of Sequences: 28952
Number of extensions: 260462
Number of successful extensions: 767
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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