Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov10a14
(627 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 38 0.005
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.005
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 37 0.010
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 36 0.017
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 36 0.022
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 36 0.029
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 35 0.051
At2g19110.1 68415.m02231 ATPase E1-E2 type family protein / halo... 31 0.47
At3g01085.1 68416.m00012 protein kinase family protein contains ... 29 2.5
At5g53390.1 68418.m06634 expressed protein 28 4.4
At3g16480.1 68416.m02103 mitochondrial processing peptidase alph... 28 4.4
At2g33160.1 68415.m04063 glycoside hydrolase family 28 protein /... 28 4.4
At2g47520.1 68415.m05931 AP2 domain-containing transcription fac... 28 5.8
At1g31460.1 68414.m03852 expressed protein 28 5.8
At5g39420.1 68418.m04775 protein kinase family protein contains ... 27 7.7
At5g23630.1 68418.m02771 ATPase E1-E2 type family protein / halo... 27 7.7
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.9 bits (84), Expect = 0.005
Identities = 37/148 (25%), Positives = 66/148 (44%), Gaps = 13/148 (8%)
Frame = +2
Query: 179 FSVFSRDPFFRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DVQNFT 346
F FS+D + DP + +S + ++ V + + +VD K+ E H+ D+
Sbjct: 16 FDPFSQDLW--DPFEGFFTPSSALANASTARDVAAFTNARVDWKETPEAHVFKADLPGLK 73
Query: 347 PEQIQVKTVG-NEIMIEGKKEIKRE---DGWTR-----SHFERRFLLPEGFPPERVECHL 499
E+++V+ N + I G++ + E D W R F RRF LPE E V+ +
Sbjct: 74 KEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATM 133
Query: 500 DKGKLKLIAFRAEPLQERAVPIQEKSVN 583
+ G L ++ +A + + I N
Sbjct: 134 ENGVLTVVVPKAPEKKPQVKSIDISGAN 161
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.9 bits (84), Expect = 0.005
Identities = 29/139 (20%), Positives = 61/139 (43%), Gaps = 9/139 (6%)
Frame = +2
Query: 206 FRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKVEVHLDVQNFTPEQIQVKTVGNEI 385
F DP K + + +E + + + K + E+ LD+ ++++++ N +
Sbjct: 46 FPDPFKILERIPLGLERDTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGV 105
Query: 386 M-IEGKK---EIKREDGWTR-----SHFERRFLLPEGFPPERVECHLDKGKLKLIAFRAE 538
+ + G++ E K+ D W R F R+F LP+ E V+ L+ G L + +
Sbjct: 106 LRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINLTKLS 165
Query: 539 PLQERAVPIQEKSVNSEQT 595
P + + + + +QT
Sbjct: 166 PEKVKGPRVVNIAAEEDQT 184
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 37.1 bits (82), Expect = 0.010
Identities = 37/148 (25%), Positives = 73/148 (49%), Gaps = 13/148 (8%)
Frame = +2
Query: 179 FSVFSRDPFFRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DVQNFT 346
F FS D + DP K ++ +S ++ I + +VD K+ E H+ D+
Sbjct: 18 FDPFSLDVW--DPFKELQFPSSLSGETSAI------TNARVDWKETAEAHVFKADLPGMK 69
Query: 347 PEQIQVKTVGNEIM-IEGKKEIKRE---DGWTR-----SHFERRFLLPEGFPPERVECHL 499
E+++V+ + ++ I G++ +++E D W R F R+F LPE ++V+ +
Sbjct: 70 KEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASM 129
Query: 500 DKGKLKLIAFRAEPLQERAVPIQEKSVN 583
+ G L + + E +++A Q KS++
Sbjct: 130 ENGVLTVTVPKVEEAKKKA---QVKSID 154
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 36.3 bits (80), Expect = 0.017
Identities = 38/129 (29%), Positives = 59/129 (45%), Gaps = 13/129 (10%)
Frame = +2
Query: 173 DPFSVFSRDPFFRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DVQN 340
DPFS+ DPF + +P +D V + + KVD ++ E H+ DV
Sbjct: 17 DPFSLDVWDPF--EGFLTPGLTNAPAKD------VAAFTNAKVDWRETPEAHVFKADVPG 68
Query: 341 FTPEQIQVKTV-GNEIMIEGKKEIKRE---DGWTR-----SHFERRFLLPEGFPPERVEC 493
E+++V+ GN + I G++ + E D W R F RRF LPE E V+
Sbjct: 69 LKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVEEVKA 128
Query: 494 HLDKGKLKL 520
++ G L +
Sbjct: 129 SMENGVLSV 137
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 35.9 bits (79), Expect = 0.022
Identities = 35/142 (24%), Positives = 67/142 (47%), Gaps = 13/142 (9%)
Frame = +2
Query: 197 DPFFRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DVQNFTPEQIQV 364
DPF D ++ P S I + +VD K+ E H+ D+ E+++V
Sbjct: 20 DPFSLDVWDPFKELQFPSSSSSAI------ANARVDWKETAEAHVFKADLPGMKKEEVKV 73
Query: 365 KTVGNEIM-IEGKKEIKRE---DGWTRSH-----FERRFLLPEGFPPERVECHLDKGKLK 517
+ + ++ I G++ +++E D W R F R+F LPE ++V+ ++ G L
Sbjct: 74 EIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASMENGVLT 133
Query: 518 LIAFRAEPLQERAVPIQEKSVN 583
+ + E +++A Q KS++
Sbjct: 134 VTVPKVETNKKKA---QVKSID 152
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 35.5 bits (78), Expect = 0.029
Identities = 39/131 (29%), Positives = 60/131 (45%), Gaps = 15/131 (11%)
Frame = +2
Query: 173 DPFSVFSRDPF--FRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DV 334
DPFS+ DPF F P A +P D V + + KVD ++ E H+ D+
Sbjct: 17 DPFSLDVFDPFEGFLTPSGL---ANAPAMD------VAAFTNAKVDWRETPEAHVFKADL 67
Query: 335 QNFTPEQIQVKTV-GNEIMIEGKKEIKRE---DGWTR-----SHFERRFLLPEGFPPERV 487
E+++V+ GN + I G++ + E D W R F RRF LPE E +
Sbjct: 68 PGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEI 127
Query: 488 ECHLDKGKLKL 520
+ ++ G L +
Sbjct: 128 KASMENGVLSV 138
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 34.7 bits (76), Expect = 0.051
Identities = 36/143 (25%), Positives = 67/143 (46%), Gaps = 13/143 (9%)
Frame = +2
Query: 179 FSVFSRDPFFRDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKV-EVHL---DVQNFT 346
F FS D + DP K + ++ E+S + +VD ++ E H+ D+
Sbjct: 17 FDPFSLDVW--DPFKELTSSSLSRENSAIVNA-------RVDWRETPEAHVFKADLPGLK 67
Query: 347 PEQIQVKTVGNEIM-IEGKKEIKREDG---WTR-----SHFERRFLLPEGFPPERVECHL 499
E+++V+ + ++ I G++ +++ED W R F RRF LPE ++V+ +
Sbjct: 68 KEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAM 127
Query: 500 DKGKLKLIAFRAEPLQERAVPIQ 568
+ G L + +AE + IQ
Sbjct: 128 ENGVLTVTVPKAETKKADVKSIQ 150
>At2g19110.1 68415.m02231 ATPase E1-E2 type family protein /
haloacid dehalogenase-like hydrolase family protein /
heavy-metal-associated domain-containing protein similar
to cadmium efflux pump protein from Geobacillus
stearothermophilus [GI:16753175], cadmium resistance
protein B from Staphylococcus aureus [GI:14020985];
T20K24.13 has been merged with T20K24.12 per suggestion
of Dr. Kristian Axelsen (axe@biobase.dk)
Length = 1172
Score = 31.5 bits (68), Expect = 0.47
Identities = 26/81 (32%), Positives = 35/81 (43%), Gaps = 2/81 (2%)
Frame = +2
Query: 179 FSVFSRDPFFRDPIKYIRQATSPIEDSHGIEGVYSD--SEIKVDGKKVEVHLDVQNFTPE 352
F SRD R + ++ S + SH + D + V+ + EV D QNF E
Sbjct: 416 FKSLSRDINLRSLLYWVSSVES--KSSHPMAATIVDYAKSVSVEPRPEEVE-DYQNFPGE 472
Query: 353 QIQVKTVGNEIMIEGKKEIKR 415
I K GN+I I KK R
Sbjct: 473 GIYGKIDGNDIFIGNKKIASR 493
>At3g01085.1 68416.m00012 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain; contains
non-consensus splice site (GC) at intron 2
Length = 629
Score = 29.1 bits (62), Expect = 2.5
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 141 RTGIRLLEHLKILSPSSPGTLSSAIRSSTSGKPPH 245
+T I LLE+L + P GT SSA+ S P+
Sbjct: 371 KTAINLLENLLSIDPEKRGTASSALMSEYFNTQPY 405
>At5g53390.1 68418.m06634 expressed protein
Length = 486
Score = 28.3 bits (60), Expect = 4.4
Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +2
Query: 212 DPIKYIRQATSPIE-DSHGIEGVYSDSEIKV 301
DP++Y+RQA + I+ H +E V+S + K+
Sbjct: 347 DPLEYVRQAKATIDRKKHSLEAVFSMAFFKL 377
>At3g16480.1 68416.m02103 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 499
Score = 28.3 bits (60), Expect = 4.4
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 10/66 (15%)
Frame = +2
Query: 239 TSPIEDSHGIEGVYSDSEIKVDGKKVEVHLD----------VQNFTPEQIQVKTVGNEIM 388
TSP S GIE V S+ DGK + HLD + N I + +G +I+
Sbjct: 383 TSPEFASQGIELVASEMNAVADGKVNQKHLDRAKAATKSAILMNLESRMIAAEDIGRQIL 442
Query: 389 IEGKKE 406
G+++
Sbjct: 443 TYGERK 448
>At2g33160.1 68415.m04063 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Salix gilgiana] GI:6714524; contains
Pfam profile PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 664
Score = 28.3 bits (60), Expect = 4.4
Identities = 17/43 (39%), Positives = 21/43 (48%)
Frame = +2
Query: 266 IEGVYSDSEIKVDGKKVEVHLDVQNFTPEQIQVKTVGNEIMIE 394
I G IK K V + V NF E IQ+ VGN I+I+
Sbjct: 273 INGTTDGLRIKTWAKSVS-QISVSNFLYENIQMINVGNPIVID 314
>At2g47520.1 68415.m05931 AP2 domain-containing transcription
factor, putative
Length = 171
Score = 27.9 bits (59), Expect = 5.8
Identities = 21/87 (24%), Positives = 37/87 (42%), Gaps = 2/87 (2%)
Frame = +2
Query: 209 RDPIKYIRQATSPIEDSHGIEGVYSDSEIKVDGKKVEVHLDVQNFTPEQIQVKTVG--NE 382
RDP K +R + + Y + IK+ G+K +++ E+ K G NE
Sbjct: 66 RDPSKGVRVWLGTFKTADEAARAYDVAAIKIRGRKAKLNFP-NTQVEEEADTKPGGNQNE 124
Query: 383 IMIEGKKEIKREDGWTRSHFERRFLLP 463
++ E + E ED + R + +P
Sbjct: 125 LISENQVESLSEDLMALEDYMRFYQIP 151
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 27.9 bits (59), Expect = 5.8
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +3
Query: 162 EHLKILSPSSPGTLSSAIRSSTSGKPPHQLKTVMASKEYTQIQRSKL 302
E +I+SPSS ++ A ST +P + K S + ++ SKL
Sbjct: 164 EKRRIVSPSSSSSIDLATPPSTDSEPEKKSKPKSKSSWFDKLSPSKL 210
>At5g39420.1 68418.m04775 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 27.5 bits (58), Expect = 7.7
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +3
Query: 144 TGIRLLEHLKILSPSSPGTLSSAIRS 221
TG+ LLE L + P GT SSA+ S
Sbjct: 361 TGVYLLETLLSMEPDKRGTASSALNS 386
>At5g23630.1 68418.m02771 ATPase E1-E2 type family protein / haloacid
dehalogenase-like hydrolase familiy protein similar to
SP|O14072 Cation-transporting ATPase 4 (EC 3.6.3.-)
{Schizosaccharomyces pombe}; contains InterPro accession
IPR001757: ATPase, E1-E2 type; contains Pfam profile
PF00702: haloacid dehalogenase-like hydrolase
Length = 1179
Score = 27.5 bits (58), Expect = 7.7
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -3
Query: 433 PRPAVFSLYLFLALNHNFVTN 371
P P+VFS+YLFL+L F +
Sbjct: 1008 PHPSVFSVYLFLSLIGQFAVH 1028
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,694,177
Number of Sequences: 28952
Number of extensions: 253016
Number of successful extensions: 870
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 870
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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