Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8p11
(526 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 85 5e-18
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom... 57 1e-09
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 54 1e-08
SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual 44 1e-05
SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 39 4e-04
SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces... 39 5e-04
SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr 1|||M... 38 7e-04
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p... 36 0.003
SPCC663.09c |||short chain dehydrogenase |Schizosaccharomyces po... 33 0.026
SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces ... 32 0.046
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 32 0.046
SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 28 0.98
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 27 1.3
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 27 1.3
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb... 27 1.7
SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|c... 27 1.7
SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 25 5.2
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 25 9.1
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 85.4 bits (202), Expect = 5e-18
Identities = 44/120 (36%), Positives = 73/120 (60%)
Frame = +3
Query: 141 LVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHL 320
L GK AL+TG + GIG TAL+ ++GA+V +A R+ E+ ++ I + +
Sbjct: 40 LTGKVALVTGSSGGIGYVTALELARKGAKVYLAGRNEEKYQKVMKQIHDEVRH----SKI 95
Query: 321 IIAKCDLTSLKSVREFAQNILDTEPQINILVNNAGVMMCPKGETEDGFEIQFATNHLAHF 500
+ DL +SV + A++ + E +++ILVNNAG+M P T+DG+E+Q TN+L+H+
Sbjct: 96 RFLRLDLLDFESVYQAAESFIAKEEKLHILVNNAGIMNPPFELTKDGYELQIQTNYLSHY 155
>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 397
Score = 57.2 bits (132), Expect = 1e-09
Identities = 36/126 (28%), Positives = 67/126 (53%), Gaps = 5/126 (3%)
Frame = +3
Query: 138 KLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGH 317
K+ G ++TG ++GIG+ GA+V++ R+ ++ D +
Sbjct: 72 KMNGMVVMVTGGSSGIGQVVVEKLASLGAQVVILLRT--EPDQFTVDYIMDLRKRTKNQL 129
Query: 318 LIIAKCDLTSLKSVREFAQNILDTEP--QINILVNNAGVMMCP---KGETEDGFEIQFAT 482
+ CDL+S+ SVR+FA +D P +++++V +GV++ P + TE+G E+Q+AT
Sbjct: 130 IYTEVCDLSSMLSVRKFATKWIDCTPIRRLDMIVLCSGVLLPPFMDRQTTEEGVELQWAT 189
Query: 483 NHLAHF 500
N L +
Sbjct: 190 NFLGPY 195
>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 54.0 bits (124), Expect = 1e-08
Identities = 34/100 (34%), Positives = 55/100 (55%), Gaps = 1/100 (1%)
Frame = +3
Query: 138 KLVGKTALITGCNTGIGKETALDFYK-RGARVIMACRSLERAEEAKADIEKSCNNINETG 314
+L GKT LITG ++GIGK TA + K ++I+A R EE ++E E
Sbjct: 3 RLDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFSTVEEIAKELESK----YEVS 58
Query: 315 HLIIAKCDLTSLKSVREFAQNILDTEPQINILVNNAGVMM 434
L + K D++ LKS+ +++ I++L+NNAG+ +
Sbjct: 59 VLPL-KLDVSDLKSIPGVIESLPKEFADIDVLINNAGLAL 97
>SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual
Length = 503
Score = 44.4 bits (100), Expect = 1e-05
Identities = 24/74 (32%), Positives = 40/74 (54%)
Frame = +3
Query: 159 LITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLIIAKCD 338
L+TG + G+GK A + RGA V + R++ + +EA A++ S I+E + D
Sbjct: 26 LVTGGSQGLGKAIAKELVLRGANVTIVARTVTKLQEAVAELSDS--KIHEDQQVSFESVD 83
Query: 339 LTSLKSVREFAQNI 380
LTS +SV + +
Sbjct: 84 LTSYESVHSMIERL 97
>SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 39.1 bits (87), Expect = 4e-04
Identities = 35/130 (26%), Positives = 61/130 (46%), Gaps = 5/130 (3%)
Frame = +3
Query: 126 ISTVKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLER--AEEAKADIEKSCNN 299
+ + KL K L+TG ++GIGK A+ F + G+ ++++C ER AE + IE+ N
Sbjct: 35 VGSGKLAEKKTLLTGGDSGIGKAAAVMFAREGSDLVISCLPEERDDAEVTRDLIEREGRN 94
Query: 300 INETGHLIIAKCDLTSLKSVREFAQNILDTEPQINILVNNAG---VMMCPKGETEDGFEI 470
I + L + R+ L I++LVNN V + ++ +++
Sbjct: 95 ------CWIWEGKLDKSDNCRDLVDFALKKLGWIDVLVNNIAYQQVAQSIEDIDDEQWDL 148
Query: 471 QFATNHLAHF 500
F TN + F
Sbjct: 149 TFKTNIFSFF 158
>SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 338
Score = 38.7 bits (86), Expect = 5e-04
Identities = 33/102 (32%), Positives = 52/102 (50%), Gaps = 10/102 (9%)
Frame = +3
Query: 150 KTALITGCNTGIGKETA---LDFYKRGAR-------VIMACRSLERAEEAKADIEKSCNN 299
K ALITG N+G+G A L FY+ + VI+ CRS E+AE+A +++
Sbjct: 5 KYALITGSNSGLGFGIATRLLQFYQPRLQDEPEVFTVILTCRSREKAEDACRRLKEFFP- 63
Query: 300 INETGHLIIAKCDLTSLKSVREFAQNILDTEPQINILVNNAG 425
+ L DL+++ SV Q+I P+++ + NAG
Sbjct: 64 -DRKIRLEYVLLDLSNMASVEAAVQDIATRFPKLDFVYLNAG 104
>SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 38.3 bits (85), Expect = 7e-04
Identities = 25/93 (26%), Positives = 44/93 (47%)
Frame = +3
Query: 147 GKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLII 326
G A++TG GIGKE A G V++ R+ E+ + ++E T +
Sbjct: 57 GYWAVVTGATDGIGKEYATQLAMSGFNVVLISRTQEKLDALAKELETVAKVKTRTIAIDY 116
Query: 327 AKCDLTSLKSVREFAQNILDTEPQINILVNNAG 425
K T+ ++ + Q+++ T I +L+NN G
Sbjct: 117 TK---TTAETFEKLHQDLVGT--PITVLINNVG 144
>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 254
Score = 36.3 bits (80), Expect = 0.003
Identities = 30/95 (31%), Positives = 46/95 (48%), Gaps = 1/95 (1%)
Frame = +3
Query: 147 GKTALITGCNTGIGKETALDFY-KRGARVIMACRSLERAEEAKADIEKSCNNINETGHLI 323
G +I G N GIG + K G V + R A E K D K+ +N+ H+I
Sbjct: 6 GIVYVIVGGNRGIGLSLVKELSNKEGVTVFASARGPGSASELK-DWSKTHSNV----HII 60
Query: 324 IAKCDLTSLKSVREFAQNILDTEPQINILVNNAGV 428
K D+TSL+S ++ A + I++L N+G+
Sbjct: 61 --KLDVTSLRSAKDAAMQVEKVVKCIDVLWVNSGI 93
>SPCC663.09c |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 253
Score = 33.1 bits (72), Expect = 0.026
Identities = 28/94 (29%), Positives = 42/94 (44%), Gaps = 1/94 (1%)
Frame = +3
Query: 150 KTALITGCNTGIGKETALDFYKR-GARVIMACRSLERAEEAKADIEKSCNNINETGHLII 326
K I G N GIG + R G V + R E A E + + KS N+
Sbjct: 6 KVYFIAGGNRGIGLSLVKELSSREGTTVFASARKPEAATELQ-EWSKSHPNVKTV----- 59
Query: 327 AKCDLTSLKSVREFAQNILDTEPQINILVNNAGV 428
+ D+TS +S E AQ++ I++L N+G+
Sbjct: 60 -ELDVTSQQSANEAAQSVAKAVDGIDVLWLNSGI 92
>SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 261
Score = 32.3 bits (70), Expect = 0.046
Identities = 28/105 (26%), Positives = 44/105 (41%)
Frame = +3
Query: 114 SGRCISTVKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSC 293
S + + L GK A++ G GIG F + GA + + E+A +I ++
Sbjct: 10 SRNILDLLSLKGKNAVVFGGARGIGHAICSVFAEAGANAFIVYNTTP-GEKAAKEIAQA- 67
Query: 294 NNINETGHLIIAKCDLTSLKSVREFAQNILDTEPQINILVNNAGV 428
N + KCD+T K V I I+I+V N G+
Sbjct: 68 NGVKT----YTCKCDVTIPKEVEHAFAEIQKVFDTIDIVVPNNGI 108
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 32.3 bits (70), Expect = 0.046
Identities = 29/111 (26%), Positives = 53/111 (47%), Gaps = 1/111 (0%)
Frame = +3
Query: 129 STVKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINE 308
S L GKT LITG + GIG A F G+ V + ++A E A++ + + +
Sbjct: 3 SMFSLKGKTTLITGGSGGIGFSIAKAFAAAGSNVGLLYGRNKKALEYAAEL-RDKHGVQA 61
Query: 309 TGHLIIAKCDLTSLKSVREFA-QNILDTEPQINILVNNAGVMMCPKGETED 458
+ C + + +V E Q + + ++++++ NAG+ + P ED
Sbjct: 62 KAY----SCPIENRSAVIETTNQAVEELGGRLDVMIANAGIAI-PHLSLED 107
>SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 292
Score = 27.9 bits (59), Expect = 0.98
Identities = 30/97 (30%), Positives = 44/97 (45%)
Frame = +3
Query: 135 VKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETG 314
+K T LITG + G+G +G VI AC RA + IE S
Sbjct: 1 MKSTFSTVLITGSSKGLGYALVKVGLAQGYNVI-AC---SRAPDT-ITIEHS-------- 47
Query: 315 HLIIAKCDLTSLKSVREFAQNILDTEPQINILVNNAG 425
L+ K D+T +KSV ++ ++I++NNAG
Sbjct: 48 KLLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNAG 84
>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 27.5 bits (58), Expect = 1.3
Identities = 24/94 (25%), Positives = 41/94 (43%)
Frame = +3
Query: 147 GKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLII 326
G+ LITG GIGK F + G RV A DI + + + +
Sbjct: 5 GRVVLITGAAGGIGKVLCKMFTELGDRV------------AGIDIVDP-SKVQDAA--LA 49
Query: 327 AKCDLTSLKSVREFAQNILDTEPQINILVNNAGV 428
+ D++ + + ++ T I++L+NNAG+
Sbjct: 50 LQADVSKADQIETAIEKVIQTLGPIDVLINNAGL 83
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 27.5 bits (58), Expect = 1.3
Identities = 15/72 (20%), Positives = 36/72 (50%)
Frame = +3
Query: 159 LITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLIIAKCD 338
L+ + GI +T +FY ++ ++ L + + AD++++ + +T + ++K
Sbjct: 153 LLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVDLPTADVDRTLIQVQQTFDIPMSKPI 212
Query: 339 LTSLKSVREFAQ 374
L S K+ + Q
Sbjct: 213 LVSSKTGKNVEQ 224
>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 247
Score = 27.1 bits (57), Expect = 1.7
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +3
Query: 150 KTALITGCNTGIGKETALDFYKRGARVIMACRSL 251
K L+TG + GIG TA K+ A+VI RSL
Sbjct: 7 KVILLTGSSKGIGLATAEALQKK-AKVIAVSRSL 39
>SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 329
Score = 27.1 bits (57), Expect = 1.7
Identities = 12/47 (25%), Positives = 20/47 (42%)
Frame = +2
Query: 359 TRICTKHFGYRTTNQYPC*QCRSDDVSQGRN*RWFRNTVCYKPFSSF 499
+R C K Y+ + C QC + + WF+N V ++ F
Sbjct: 96 SRFCEKCQEYKCDRSHHCSQCNKCILRMDHHCMWFKNCVGFRNHKFF 142
>SPBC1685.07c |||amino acid transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 25.4 bits (53), Expect = 5.2
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -1
Query: 487 WFVANCISKPSSVSPLGHIITPALLTRILICGSVSKMFCAN 365
+F ++ ++ P + SPL I+ A + I G+V + C N
Sbjct: 372 YFFSHKVASPGNSSPLQLRISRAFAAGLAIYGTVVMILCLN 412
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 24.6 bits (51), Expect = 9.1
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 237 ACRSLERAEEAKADIEKSCNNINETGHLIIAKCDLTS 347
+CR ++R+ + CN++ + L IAKC LTS
Sbjct: 299 SCRQIKRSS-----VVSLCNSVFDGKKLTIAKCLLTS 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,091,579
Number of Sequences: 5004
Number of extensions: 40581
Number of successful extensions: 121
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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