Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc8j09
         (755 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    44   3e-05
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...    37   0.004
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    28   1.7  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    28   1.7  
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S...    26   5.0  
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa...    26   5.0  
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo...    26   5.0  
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ...    26   6.7  
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    26   6.7  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   8.8  

>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 42/148 (28%), Positives = 60/148 (40%)
 Frame = +1

Query: 274 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 453
           EG  M+L   A PL           +  KD  A  +     +K G  R DLL AN+ IFK
Sbjct: 59  EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117

Query: 454 EQGQALDKAARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAK 633
           E  + + K   KD  +LV  NP +       K      +    + T +D  R Q  +   
Sbjct: 118 EILREVTKYT-KDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKL 176

Query: 634 IGVPVKDVKRVIIWGNHSSTQFPDASNA 717
            G+  + V   II G H  ++    S+A
Sbjct: 177 YGLDPQSVNADII-GEHGDSELAVWSHA 203


>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score = 36.7 bits (81), Expect = 0.004
 Identities = 20/53 (37%), Positives = 27/53 (50%)
 Frame = +1

Query: 466 ALDKAARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL 624
           A+  +   D K +VV + +   A +CSKY PSIP      +TR  Q   QS L
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHL 443


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = -1

Query: 407 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 234
           P F  + P RK +A  T+L  SS       P  + RA SANS+T+P   P   A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573

Query: 233 KT 228
            T
Sbjct: 574 TT 575


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
 Frame = -1

Query: 512 PTTRTFTSLRAALSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 348
           P+  T + L  +   + P S    T A   S   + S      T  ++     +T   ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580

Query: 347 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 261
           SG     T   SG    + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609


>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
           Klp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 784

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 22/102 (21%), Positives = 45/102 (44%), Gaps = 2/102 (1%)
 Frame = +1

Query: 424 LLAANVRIFKEQGQALDKAARKDVKV--LVVGNPANTNALICSKYAPSIPKENFTAMTRL 597
           ++   +R   ++ + L +    D+ V  L + N    + L+    +   PK +       
Sbjct: 153 IIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPK-SLNICEDA 211

Query: 598 DQNRAQSQLAAKIGVPVKDVKRVIIWGNHSSTQFPDASNAVA 723
           +QN +   L+      +++V  +II GN + T  P  +NAV+
Sbjct: 212 EQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVS 253


>SPCC18B5.03 |wee1||dual specificity protein kinase
           Wee1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 877

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 23/74 (31%), Positives = 26/74 (35%), Gaps = 9/74 (12%)
 Frame = +1

Query: 517 PANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL---------AAKIGVPVKDVKRVI 669
           P N+N    S    SIP    T       N AQS L         A K+G      K  +
Sbjct: 240 PPNSNNANPSTLFSSIPSSRHTTSNHFPSNSAQSSLFSPTARPLTARKLGFASSQTKSAV 299

Query: 670 IWGNHSSTQFPDAS 711
              NHS     DAS
Sbjct: 300 S-NNHSRNSSKDAS 312


>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 571

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = -1

Query: 566 GIDGAYLEHIRAFVLAGLPTTR 501
           G+ G+Y EH+    + G+P+T+
Sbjct: 87  GVAGSYAEHVPVVHIVGMPSTK 108


>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
           Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = -1

Query: 692 VDEWFPQMITLLT 654
           VDEWFP+M T LT
Sbjct: 231 VDEWFPKMETSLT 243


>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 594

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -1

Query: 566 GIDGAYLEHIRAFVLAGLPTTRTFTS 489
           GI GAY E++   +++G P T   +S
Sbjct: 89  GIGGAYAENLPVILVSGSPNTNDLSS 114


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -1

Query: 314 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 192
           + R+ S N   TPSS+P   + SR +       PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,959,744
Number of Sequences: 5004
Number of extensions: 60322
Number of successful extensions: 194
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)