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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc8j03
         (656 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    29   0.78 
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit...    26   5.5  
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar...    26   5.5  
SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual    25   7.3  

>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 28.7 bits (61), Expect = 0.78
 Identities = 17/45 (37%), Positives = 24/45 (53%)
 Frame = -2

Query: 211 RSDSDSKFVSVCSASLASCLKLHDDRQ*KYSSTSMTTPISLFKCS 77
           +S SDS   S  S+S +  + L+DD    YSS +  T IS +  S
Sbjct: 112 KSYSDSSTSSARSSSTSGSISLYDDYYPAYSSNAPNTAISNYVSS 156


>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
           Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 161

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 7/15 (46%), Positives = 10/15 (66%)
 Frame = +3

Query: 12  WCDFHRNHAYRGTRD 56
           WC+ H+N  Y GT +
Sbjct: 57  WCEHHKNDLYSGTEE 71


>SPAPB1A10.07c |||sphingolipid biosynthesis
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
 Frame = +3

Query: 96  MGVVMLVEEY-FYCLSSCNFKQ 158
           +G+V+ +  Y F+C SSC+F Q
Sbjct: 212 VGLVLTILTYVFFCASSCSFNQ 233


>SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 16/61 (26%), Positives = 30/61 (49%)
 Frame = +3

Query: 45  GTRDIACNNYVEHLNSDMGVVMLVEEYFYCLSSCNFKQDAKLALQTLTNFESLSDLMASY 224
           G+ ++  ++Y  H + D+GV ++    F  L     + D+K  L       ++ DL+ SY
Sbjct: 205 GSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKL-------TMQDLINSY 257

Query: 225 N 227
           N
Sbjct: 258 N 258


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,530,763
Number of Sequences: 5004
Number of extensions: 49818
Number of successful extensions: 112
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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