BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8j03
(656 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 29 0.78
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit... 26 5.5
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 5.5
SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual 25 7.3
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 28.7 bits (61), Expect = 0.78
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = -2
Query: 211 RSDSDSKFVSVCSASLASCLKLHDDRQ*KYSSTSMTTPISLFKCS 77
+S SDS S S+S + + L+DD YSS + T IS + S
Sbjct: 112 KSYSDSSTSSARSSSTSGSISLYDDYYPAYSSNAPNTAISNYVSS 156
>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 161
Score = 25.8 bits (54), Expect = 5.5
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = +3
Query: 12 WCDFHRNHAYRGTRD 56
WC+ H+N Y GT +
Sbjct: 57 WCEHHKNDLYSGTEE 71
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
Frame = +3
Query: 96 MGVVMLVEEY-FYCLSSCNFKQ 158
+G+V+ + Y F+C SSC+F Q
Sbjct: 212 VGLVLTILTYVFFCASSCSFNQ 233
>SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 25.4 bits (53), Expect = 7.3
Identities = 16/61 (26%), Positives = 30/61 (49%)
Frame = +3
Query: 45 GTRDIACNNYVEHLNSDMGVVMLVEEYFYCLSSCNFKQDAKLALQTLTNFESLSDLMASY 224
G+ ++ ++Y H + D+GV ++ F L + D+K L ++ DL+ SY
Sbjct: 205 GSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKL-------TMQDLINSY 257
Query: 225 N 227
N
Sbjct: 258 N 258
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,530,763
Number of Sequences: 5004
Number of extensions: 49818
Number of successful extensions: 112
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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