Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8f15
(726 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 97 2e-21
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 79 6e-16
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 77 2e-15
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 49 6e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 46 4e-06
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 4e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 5e-04
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 38 0.002
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 32 0.096
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.51
SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce... 25 8.3
SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 25 8.3
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 97.1 bits (231), Expect = 2e-21
Identities = 58/169 (34%), Positives = 80/169 (47%), Gaps = 5/169 (2%)
Frame = +2
Query: 203 SSDVIELTPSN-FDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGA 373
+S V+EL N + + S + +IEF+A WCGHCKSL P Y++ + V +G
Sbjct: 19 ASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGK 78
Query: 374 LDADEHRSVSQKYGVTGFPTIKIF--TGSKHTPYQGQRTAEGFVXXXXXXXXXXXXXNLX 547
+DAD H V+ KY +TGFPT+ F GS+ Y R +
Sbjct: 79 IDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLT---------QFVSEKT 129
Query: 548 XXXXXXXXXXXXVITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEP 694
V+ L NF ++V+D LVEFYA WCG+CK L P
Sbjct: 130 GIKKRKIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAP 178
Score = 73.7 bits (173), Expect = 2e-14
Identities = 37/103 (35%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +2
Query: 206 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALD 379
S+V+EL NFDK+V + + ++EF+A WCG+CK L P Y+ + K V++ ++
Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKIN 199
Query: 380 ADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAEGFV 499
AD + + + V FPTIK F K P Y+G R+ E +
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLI 242
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 79.0 bits (186), Expect = 6e-16
Identities = 44/101 (43%), Positives = 60/101 (59%), Gaps = 8/101 (7%)
Frame = +2
Query: 206 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--D 379
S+ IEL NF K V ++ F+APWCG+CK LVP Y+K A L ++ V A+ D
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVV-FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCD 89
Query: 380 ADEHRSVSQKYGVTGFPTIK-IFTGSK-----HTPYQGQRT 484
AD++R+V +Y V GFPTIK ++ SK T Y G R+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRS 130
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/45 (46%), Positives = 26/45 (57%)
Frame = +2
Query: 587 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATEL 721
I L NF++ V LV FYAPWCG+CK L P + K A+ L
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGYCKKLVPTYQKLASNL 77
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 77.4 bits (182), Expect = 2e-15
Identities = 34/102 (33%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
Frame = +2
Query: 200 SSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGAL 376
+S++V ++ ++L+T +D++ +++F+APWCGHCK+L PEY+ AA L K + + +
Sbjct: 20 ASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEV 78
Query: 377 DADEHRSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAEGFV 499
D E + +Y + G+PT+ +F G + + Y G R + V
Sbjct: 79 DCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALV 120
Score = 74.9 bits (176), Expect = 1e-14
Identities = 41/103 (39%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +2
Query: 197 DSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVG 370
+S D++ L NFD +V + + ++EF+APWCGHCK+L P Y+K A V V
Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVA 411
Query: 371 ALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAE 490
+DA E+ +S ++GFPTI F K P Y+G RT E
Sbjct: 412 KIDATEN-DIS--VSISGFPTIMFFKANDKVNPVRYEGDRTLE 451
Score = 58.8 bits (136), Expect = 7e-10
Identities = 24/45 (53%), Positives = 30/45 (66%)
Frame = +2
Query: 584 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATE 718
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEE 401
Score = 49.2 bits (112), Expect = 6e-07
Identities = 18/35 (51%), Positives = 26/35 (74%)
Frame = +2
Query: 620 VLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 724
++ +D + +V+FYAPWCGHCK L P + AA EL+
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELE 69
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.2 bits (112), Expect = 6e-07
Identities = 21/64 (32%), Positives = 40/64 (62%)
Frame = +2
Query: 254 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 433
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 434 IKIF 445
+ +F
Sbjct: 92 MVLF 95
Score = 36.3 bits (80), Expect = 0.004
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 623 LDSDDLWLVEFYAPWCGHCKNLEPHWAKAATE 718
+ +D + +V+FYA WCG CK L+P K + +
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 46.4 bits (105), Expect = 4e-06
Identities = 22/94 (23%), Positives = 49/94 (52%), Gaps = 1/94 (1%)
Frame = +2
Query: 212 VIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEH 391
V +++ S+ K + D++ +++FFA WCG CK++ P++++ + + +D D+
Sbjct: 2 VKQVSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQL 60
Query: 392 RSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAE 490
++ + GV P+ ++ G K G A+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAK 94
Score = 35.5 bits (78), Expect = 0.008
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 602 SNFKELVLDSDDLWLVEFYAPWCGHCKNLEP 694
S FK +V D L +V+F+A WCG CK + P
Sbjct: 9 SEFKSIVCQ-DKLVVVDFFATWCGPCKAIAP 38
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.9 bits (89), Expect = 4e-04
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +2
Query: 275 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 451
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 452 SK 457
K
Sbjct: 84 GK 85
Score = 29.9 bits (64), Expect = 0.39
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 647 VEFYAPWCGHCKNLEPHWAKAATE 718
V+ YA WCG CK + P +++ A++
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASK 47
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 39.5 bits (88), Expect = 5e-04
Identities = 20/83 (24%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +2
Query: 215 IELT-PSNFDKLVTNSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADE 388
+E+T F +++ N E I ++ F+APW CK + + + A+ K V + ++A++
Sbjct: 3 VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEK 61
Query: 389 HRSVSQKYGVTGFPTIKIFTGSK 457
+++ + V P + G+K
Sbjct: 62 FSDIAESFDVNAVPLFVLIHGAK 84
Score = 28.7 bits (61), Expect = 0.89
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +2
Query: 608 FKELVLDS-DDLWLVEFYAPWCGHCKNLEPHWAKAATELK 724
F+E++ + + + L+ FYAPW CK + + + A + K
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTK 50
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +2
Query: 587 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 724
+ LTD++ + V S W +++Y P CG CK L P W + K
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAK 72
Score = 37.1 bits (82), Expect = 0.003
Identities = 18/82 (21%), Positives = 36/82 (43%)
Frame = +2
Query: 230 SNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQK 409
++ D +T+ E W I+F++ C C + + A ++G + V ++ + ++
Sbjct: 289 ADIDAALTDK-EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQ 347
Query: 410 YGVTGFPTIKIFTGSKHTPYQG 475
Y + FPT F Y G
Sbjct: 348 YSIQYFPTFLFFKEEAFVEYVG 369
Score = 30.3 bits (65), Expect = 0.29
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 3/80 (3%)
Frame = +2
Query: 215 IELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DAD 385
+ LT ++ + V+ W I+++ P CG CK L P + K V+ + D
Sbjct: 29 VPLTDNDLESEVSKGT--WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 386 EHRSVSQKYGVTGFPTIKIF 445
+ +S + PT+ ++
Sbjct: 87 CSKELSSCANIRAVPTLYLY 106
Score = 26.6 bits (56), Expect = 3.6
Identities = 8/37 (21%), Positives = 17/37 (45%)
Frame = +2
Query: 614 ELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 724
+ L + W ++FY+ C C ++ W A ++
Sbjct: 292 DAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMR 328
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 31.9 bits (69), Expect = 0.096
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +2
Query: 176 TGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFF 286
TGS + SDV+ L+ S L+ SD WII FF
Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 29.5 bits (63), Expect = 0.51
Identities = 13/60 (21%), Positives = 24/60 (40%)
Frame = +2
Query: 296 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 475
C C + R +K+ ++ DE + + + + FPT ++F G Y G
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
>SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 459
Score = 25.4 bits (53), Expect = 8.3
Identities = 14/54 (25%), Positives = 21/54 (38%)
Frame = -1
Query: 393 LCSSASKAPTFTMPFRALAAFLYSGTRLLQCPHHGAKNSMIQISSEFVTNLSKL 232
LC K T + + F +CPH G KN+ +Q + SK+
Sbjct: 37 LCMECGKNGTTKLLLTVIPYFREVVLMSFECPHCGFKNAQVQHAETIQPEGSKI 90
>SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 138
Score = 25.4 bits (53), Expect = 8.3
Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
Frame = +2
Query: 302 HCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGV-TGFPTIKIFTG 451
HC KAAR L I++ ++ E S+SQK + PT+ I G
Sbjct: 69 HCTYSQVRGPKAARVLSEILRNRITESKEKLSLSQKEKLFQNLPTVYILHG 119
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,920,922
Number of Sequences: 5004
Number of extensions: 58548
Number of successful extensions: 188
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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