Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8e19
(361 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42300.1 68418.m05148 ubiquitin family protein contains INTER... 128 1e-30
At3g45180.1 68416.m04876 ubiquitin family protein contains INTER... 126 6e-30
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 34 0.025
At1g53950.1 68414.m06145 ubiquitin family protein contains INTER... 28 1.6
At3g17570.1 68416.m02243 F-box family protein contains Pfam PF00... 27 2.8
At5g57860.3 68418.m07237 ubiquitin family protein contains Pfam ... 27 3.8
At5g57860.2 68418.m07236 ubiquitin family protein contains Pfam ... 27 3.8
At5g57860.1 68418.m07235 ubiquitin family protein contains Pfam ... 27 3.8
At4g11230.1 68417.m01819 respiratory burst oxidase, putative / N... 27 3.8
At3g17270.1 68416.m02208 F-box family protein 26 6.6
At1g57650.1 68414.m06542 disease resistance protein (NBS-LRR cla... 26 6.6
At1g80070.1 68414.m09373 splicing factor, putative strong simila... 26 8.7
At1g77870.1 68414.m09075 expressed protein similar to geranylger... 26 8.7
At1g43780.1 68414.m05043 serine carboxypeptidase S10 family prot... 26 8.7
>At5g42300.1 68418.m05148 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 73
Score = 128 bits (308), Expect = 1e-30
Identities = 55/72 (76%), Positives = 63/72 (87%)
Frame = +1
Query: 112 MLEVTCNDRLGKKVRVKCNPDDTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKDHIKLADY 291
M+EV NDRLGKKVRVKCN DDT+GDLKKL+AAQTGTR +KI ++KWY ++KDHI L DY
Sbjct: 1 MIEVVLNDRLGKKVRVKCNDDDTIGDLKKLVAAQTGTRAEKIRIQKWYNIYKDHITLKDY 60
Query: 292 EIHDGMNLELYY 327
EIHDGM LELYY
Sbjct: 61 EIHDGMGLELYY 72
>At3g45180.1 68416.m04876 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 73
Score = 126 bits (303), Expect = 6e-30
Identities = 54/72 (75%), Positives = 63/72 (87%)
Frame = +1
Query: 112 MLEVTCNDRLGKKVRVKCNPDDTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKDHIKLADY 291
M+EV NDRLGKKVRVKCN +DT+GDLKKL+AAQTGTR +KI ++KWY ++KDHI L DY
Sbjct: 1 MIEVVLNDRLGKKVRVKCNEEDTIGDLKKLVAAQTGTRPEKIRIQKWYNIYKDHIPLKDY 60
Query: 292 EIHDGMNLELYY 327
EIHDGM LELYY
Sbjct: 61 EIHDGMGLELYY 72
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 34.3 bits (75), Expect = 0.025
Identities = 17/60 (28%), Positives = 32/60 (53%)
Frame = +1
Query: 142 GKKVRVKCNPDDTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKDHIKLADYEIHDGMNLEL 321
GK + ++ DT+ ++K+ I + G + D+ +L + +D + L DY+IH L L
Sbjct: 561 GKTIILEVESSDTIANVKEKIQVKEGIKPDQQMLIFFGQQLEDGVTLGDYDIHKKSTLYL 620
Score = 26.6 bits (56), Expect = 5.0
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +1
Query: 142 GKKVRVKCNPDDTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKDHIKLADYEIHDGMNLEL 321
GK + ++ DT+ +K I + G+ D+ +L +D L DY I + L L
Sbjct: 402 GKIITLEVLSSDTIKSVKAKIQDKVGSPPDQQILLFRGGQLQDGRTLGDYNIRNESTLHL 461
Query: 322 YY 327
++
Sbjct: 462 FF 463
>At1g53950.1 68414.m06145 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 216
Score = 28.3 bits (60), Expect = 1.6
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 175 DTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKDHIKLADYEIHDGMNL 315
DT+ LK +I Q G D+ L T K++ ++DY I NL
Sbjct: 161 DTIKKLKSMIHDQGGPPVDQQDLNHLGTKLKNNATISDYNIKPDANL 207
>At3g17570.1 68416.m02243 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain
Length = 381
Score = 27.5 bits (58), Expect = 2.8
Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 6/37 (16%)
Frame = +1
Query: 232 KIVLKKWYTVFKD------HIKLADYEIHDGMNLELY 324
K K+WYT+FKD H+ A+ E+ M+L +Y
Sbjct: 26 KPTCKRWYTLFKDPEFLKKHVGRAEREVISLMSLRVY 62
>At5g57860.3 68418.m07237 ubiquitin family protein contains Pfam
profile PF00240: Ubiquitin family
Length = 95
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +1
Query: 157 VKCNPDDTVGDLK-KLIA-AQTGTRYDKIVLKKWYTVFKDHIKLADYEIHD 303
++C+P +TV D+K KL A + ++VL V +D LAD ++ +
Sbjct: 16 IQCDPTETVLDVKQKLFALIEQPVNNQRLVLMSTEEVLEDSKSLADQKVEN 66
>At5g57860.2 68418.m07236 ubiquitin family protein contains Pfam
profile PF00240: Ubiquitin family
Length = 95
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +1
Query: 157 VKCNPDDTVGDLK-KLIA-AQTGTRYDKIVLKKWYTVFKDHIKLADYEIHD 303
++C+P +TV D+K KL A + ++VL V +D LAD ++ +
Sbjct: 16 IQCDPTETVLDVKQKLFALIEQPVNNQRLVLMSTEEVLEDSKSLADQKVEN 66
>At5g57860.1 68418.m07235 ubiquitin family protein contains Pfam
profile PF00240: Ubiquitin family
Length = 95
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +1
Query: 157 VKCNPDDTVGDLK-KLIA-AQTGTRYDKIVLKKWYTVFKDHIKLADYEIHD 303
++C+P +TV D+K KL A + ++VL V +D LAD ++ +
Sbjct: 16 IQCDPTETVLDVKQKLFALIEQPVNNQRLVLMSTEEVLEDSKSLADQKVEN 66
>At4g11230.1 68417.m01819 respiratory burst oxidase, putative /
NADPH oxidase, putative similar to respiratory burst
oxidase homolog F [gi:3242456], RbohAp108 [gi:2654868]
from Arabidopsis thaliana, respiratory burst oxidase
homolog [GI:16549087] from Solanum tuberosum; contains
Pfam profile PF01794 Ferric reductase like transmembrane
component
Length = 941
Score = 27.1 bits (57), Expect = 3.8
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -3
Query: 263 NTVYHFLSTILSYLVPVCAAINFFKSPTVS 174
NT+ + ST LS+ VP INF K+ +V+
Sbjct: 433 NTITYLRSTALSHSVPFDDCINFHKTISVA 462
>At3g17270.1 68416.m02208 F-box family protein
Length = 134
Score = 26.2 bits (55), Expect = 6.6
Identities = 9/34 (26%), Positives = 21/34 (61%)
Frame = +1
Query: 169 PDDTVGDLKKLIAAQTGTRYDKIVLKKWYTVFKD 270
PD+ ++ + A++ ++ K + K+WY +F+D
Sbjct: 8 PDELESEILSRVPAKSLAKW-KTICKRWYALFRD 40
>At1g57650.1 68414.m06542 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 709
Score = 26.2 bits (55), Expect = 6.6
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 172 DDTVGDLKK-LIAAQTGTRYDKIVLKKWYTVFKDHIKLADYEIHDGMNLELYY 327
DD VG K+ L++ + G+ + +WY DH+ + + E + + EL +
Sbjct: 580 DDEVGIQKRGLVSCRIGSEWR---FSEWYPFITDHLYIFEVEAKEVTSTELIF 629
>At1g80070.1 68414.m09373 splicing factor, putative strong similarity
to splicing factor Prp8 [Homo sapiens] GI:3661610;
contains Pfam profile PF01398: Mov34/MPN/PAD-1 family
Length = 2382
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/36 (36%), Positives = 22/36 (61%), Gaps = 2/36 (5%)
Frame = -2
Query: 360 FFFKIQMFLL--LIVQLQIHSIVDFIVCKFNMILEY 259
FF KI + +L L+ + H+I D++ K N++L Y
Sbjct: 1030 FFEKIDLTMLNRLLRLVLDHNIADYVSAKNNVVLSY 1065
>At1g77870.1 68414.m09075 expressed protein similar to
geranylgeranylated protein ATGP4 [GI:4097567]
Length = 120
Score = 25.8 bits (54), Expect = 8.7
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +3
Query: 12 VKILNGGKITKTNKKL 59
VK++NGGKI + NK L
Sbjct: 55 VKLINGGKILENNKTL 70
>At1g43780.1 68414.m05043 serine carboxypeptidase S10 family protein
similar to serine carboxylase II-3 GB:CAA55478 GI:474392
from [Hordeum vulgare]
Length = 479
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = -3
Query: 200 NFFKSPTVSSGLHLTRTFLPSRSLHVTSSILLSNQL 93
N TV H+ PSR+LH+ +S +L +L
Sbjct: 434 NLLTFATVRGAAHMVPYSQPSRALHLFTSFVLGRKL 469
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,100,475
Number of Sequences: 28952
Number of extensions: 105048
Number of successful extensions: 295
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 295
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 469342752
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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