BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8e08
(344 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59730.1 68416.m06664 receptor lectin kinase, putative simila... 29 0.83
At1g71830.1 68414.m08301 leucine-rich repeat family protein / pr... 29 1.1
At3g62250.1 68416.m06993 ubiquitin extension protein 5 (UBQ5) / ... 28 1.9
At1g34210.1 68414.m04245 somatic embryogenesis receptor-like kin... 28 1.9
At2g47110.1 68415.m05883 ubiquitin extension protein 6 (UBQ6) / ... 27 2.5
At2g16960.1 68415.m01954 importin beta-2 subunit family protein ... 27 4.4
At5g16000.1 68418.m01871 leucine-rich repeat family protein / pr... 26 7.7
At5g15360.1 68418.m01798 hypothetical protein 26 7.7
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 26 7.7
At4g13620.1 68417.m02120 AP2 domain-containing transcription fac... 26 7.7
At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 26 7.7
At1g63810.1 68414.m07221 nucleolar RNA-associated family protein... 26 7.7
At1g30500.2 68414.m03729 CCAAT-binding transcription factor (CBF... 26 7.7
At1g30500.1 68414.m03728 CCAAT-binding transcription factor (CBF... 26 7.7
>At3g59730.1 68416.m06664 receptor lectin kinase, putative similar
to receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains pfam domains PF00139:
Legume lectins beta domain and PF00069: Protein kinase
domain
Length = 523
Score = 29.1 bits (62), Expect = 0.83
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -2
Query: 253 LHY-VLLLSSPIRALSSVRWGNLPFFPPSKEKCSDRHNRHRA*CYSIS 113
LHY VL+ + P + +G +P PP +K SDR A C +++
Sbjct: 239 LHYMVLVYTYPEAVYQPLEFGRVPTLPPYPKKPSDRLRTVLAVCLTLA 286
>At1g71830.1 68414.m08301 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 625
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/32 (34%), Positives = 15/32 (46%)
Frame = +2
Query: 95 GSNTSAANAIALGAMAVVAVGTFLFTWWKERK 190
G + A +A GA + A F WW+ RK
Sbjct: 235 GITGAIAGGVAAGAALLFAAPAIAFAWWRRRK 266
>At3g62250.1 68416.m06993 ubiquitin extension protein 5 (UBQ5) / 40S
ribosomal protein S27A (RPS27aC) identical to GI:166933,
GI:166934
Length = 157
Score = 27.9 bits (59), Expect = 1.9
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 306 KHKRVHVSIILRTKLFFRCTMFCYYLRRFAP*APCGGGTFLSFH 175
KHK+V ++++ K+ + LR+ P A CG GTF++ H
Sbjct: 94 KHKKVKLAVLQFYKIDGSGKV--QRLRKECPNATCGAGTFMASH 135
>At1g34210.1 68414.m04245 somatic embryogenesis receptor-like kinase
2 (SERK2) nearly identical to somatic embryogenesis
receptor-like kinase 2 [Arabidopsis thaliana]
GI:14573457; contains Pfam domains PF00560: Leucine Rich
Repeat and PF00069: Protein kinase domain; identical to
cDNA somatic embryogenesis receptor-like kinase 2
(SERK2) GI:14573456
Length = 628
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +2
Query: 113 ANAIALGAMAVVAVGTFLFTWWKERK 190
A +A GA + A F WW+ RK
Sbjct: 244 AGGVAAGAALLFAAPALAFAWWRRRK 269
>At2g47110.1 68415.m05883 ubiquitin extension protein 6 (UBQ6) / 40S
ribosomal protein S27A (RPS27aB) identical to GI:166936
Length = 157
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 306 KHKRVHVSIILRTKLFFRCTMFCYYLRRFAP*APCGGGTFLSFH 175
KHK+V ++++ K+ + LR+ P A CG GTF++ H
Sbjct: 94 KHKKVKLAVLQFYKVDGSGKV--QRLRKECPNATCGAGTFMASH 135
>At2g16960.1 68415.m01954 importin beta-2 subunit family protein
contains weak hit to Pfam PF02985: HEAT repeat (4
copies); contains weak hit to Pfam PF03130: PBS lyase
HEAT-like repeat (2 copies); supported by tandem
duplication of importin beta family protein
(TIGR_Ath1:At2g16950) [Arabidopsis thaliana]; similar to
Importin beta-2 subunit (Karyopherin beta-2 subunit)
(Transportin) (M9 region interaction protein) (MIP)
(Swiss-Prot:Q92973) [Homo sapiens]
Length = 547
Score = 26.6 bits (56), Expect = 4.4
Identities = 15/31 (48%), Positives = 20/31 (64%), Gaps = 2/31 (6%)
Frame = +2
Query: 59 CHFAMTKNSNSQGSNTSAAN--AIALGAMAV 145
CH +TK++N G N SAAN A+G +AV
Sbjct: 336 CH--LTKSANFSGENLSAANNACKAIGELAV 364
>At5g16000.1 68418.m01871 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 638
Score = 25.8 bits (54), Expect = 7.7
Identities = 8/33 (24%), Positives = 19/33 (57%)
Frame = +2
Query: 89 SQGSNTSAANAIALGAMAVVAVGTFLFTWWKER 187
S+ + A ++G ++++ + LF WW++R
Sbjct: 242 SRNHKMAIAVGSSVGTVSLIFIAVGLFLWWRQR 274
>At5g15360.1 68418.m01798 hypothetical protein
Length = 253
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = +2
Query: 35 RFSLPQINCHFAMTKNSNSQG---SNTSAANAIALGAMAVVAVGTF 163
RF LPQI C F+ + + S ++ +A+ L A AVV T+
Sbjct: 185 RFGLPQIACAFSNSSHLQDMSHLFSALASNSAVDLSARAVVERTTY 230
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +2
Query: 65 FAMTKNSNSQGSNTSAANAIALGAMAVVAVGTFLFTWWKERKVPP 199
F M N N + + + +A + G + LFT E+K+PP
Sbjct: 655 FKMFSNLNKKDTVSYSAMIMGCGINGMATEANSLFTAMIEKKIPP 699
>At4g13620.1 68417.m02120 AP2 domain-containing transcription
factor, putative similar to AP2 domain containing
protein RAP2.4 [Arabidopsis thaliana] GI:2281633;
contains Pfam profile PF00847: AP2 domain
Length = 388
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 238 LLSSPIRALSSVRWGNLPFFPPSKEKCSDRHNRH 137
LL S I+ +SS + N P PP K ++ N H
Sbjct: 306 LLESKIQQISSSQVSNSPSPPPPKVGTPEQKNHH 339
>At1g66880.1 68414.m07601 serine/threonine protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 1296
Score = 25.8 bits (54), Expect = 7.7
Identities = 14/58 (24%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = -2
Query: 175 PSKEKCSDRHN-RHRA*CYSISSGCV--AALRIRILGHRKMAIYLWQRKTTMIVMEHF 11
P + KC H+ + C +I++G R +I GH + ++ + ++I+ +HF
Sbjct: 401 PFEVKCKPHHSPTAKFQCGNITAGFPFSGGNRPQICGHPSLELHCYNNMASIIISDHF 458
>At1g63810.1 68414.m07221 nucleolar RNA-associated family protein /
Nrap family protein contains Pfam profile PF03813: Nrap
protein; similar to nucleolar RNA-associated protein
alpha (GI:18539461) [Mus musculus]
Length = 1053
Score = 25.8 bits (54), Expect = 7.7
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 150 RSEHFSLLGGKKGRFP 197
RS+H S++ G +GRFP
Sbjct: 726 RSQHASMINGLQGRFP 741
>At1g30500.2 68414.m03729 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
Length = 190
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 121 YSTRRDGGCGGRNISLYLVERKEGSPTARSSRRESAKIVAEHSA 252
++ RR GCGGR +L +KE SS E + + A SA
Sbjct: 143 HAIRRPRGCGGR----FLNAKKEDEHHEDSSHEEKSNLSAGKSA 182
>At1g30500.1 68414.m03728 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
Length = 186
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 121 YSTRRDGGCGGRNISLYLVERKEGSPTARSSRRESAKIVAEHSA 252
++ RR GCGGR +L +KE SS E + + A SA
Sbjct: 139 HAIRRPRGCGGR----FLNAKKEDEHHEDSSHEEKSNLSAGKSA 178
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,783,735
Number of Sequences: 28952
Number of extensions: 142566
Number of successful extensions: 390
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 382
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 390
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 419412672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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