BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8d05
(444 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B) ribo... 130 5e-31
At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C) 127 3e-30
At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A) 127 4e-30
At4g17250.1 68417.m02594 expressed protein 28 2.5
At3g19400.2 68416.m02460 cysteine proteinase, putative non-conse... 28 2.5
At3g19400.1 68416.m02461 cysteine proteinase, putative non-conse... 28 2.5
At3g51220.1 68416.m05607 expressed protein contains Pfam doamin ... 27 4.3
At1g32900.1 68414.m04053 starch synthase, putative similar to st... 27 5.7
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ... 26 10.0
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 26 10.0
>At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B)
ribosomal protein L31, Nicotiana glutinosa, U23784
Length = 119
Score = 130 bits (313), Expect = 5e-31
Identities = 62/116 (53%), Positives = 81/116 (69%), Gaps = 2/116 (1%)
Frame = +2
Query: 65 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 244
E+KG+ EVVTREYT+NLH+RLH FKK+AP+AIKEIRKFAEK+MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVVTREYTINLHRRLHSCTFKKKAPKAIKEIRKFAEKEMGTKDVRVDVKLN 60
Query: 245 KFLWSKGVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 406
K +WSKG+R P NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 61 KQIWSKGIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKIIE 116
>At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)
Length = 119
Score = 127 bits (307), Expect = 3e-30
Identities = 61/116 (52%), Positives = 80/116 (68%), Gaps = 2/116 (1%)
Frame = +2
Query: 65 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 244
E+KG+ EV+TREYT+NLH+RLH FKK+AP+AIKEIRKFAEK MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVITREYTINLHRRLHKCTFKKKAPKAIKEIRKFAEKAMGTKDVRVDVKLN 60
Query: 245 KFLWSKGVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 406
K +WSKG+R P NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 61 KQIWSKGIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIE 116
>At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A)
Length = 119
Score = 127 bits (306), Expect = 4e-30
Identities = 61/117 (52%), Positives = 77/117 (65%), Gaps = 2/117 (1%)
Frame = +2
Query: 71 KGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 250
KGK EVVTREYT+NLH+RLH FKK+AP AIKEIRKFA K MGT D+RVD +LNK
Sbjct: 3 KGKGRKEEVVTREYTINLHRRLHSCTFKKKAPNAIKEIRKFALKAMGTKDVRVDVKLNKQ 62
Query: 251 LWSKGVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVDASQ 415
+WSKG+R P NDDED+ + F+LVT +P + GL T+ ++ +
Sbjct: 63 IWSKGIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIEEEE 119
>At4g17250.1 68417.m02594 expressed protein
Length = 416
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +2
Query: 113 TVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVD 232
TV KR + +KKR A+K+ + E++ G P ++ D
Sbjct: 169 TVPATKRFLELKYKKRYEFALKQCPSYTERRRGVPKLKED 208
>At3g19400.2 68416.m02460 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 290
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +2
Query: 107 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 250
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At3g19400.1 68416.m02461 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 362
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +2
Query: 107 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 250
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At3g51220.1 68416.m05607 expressed protein contains Pfam doamin
PF05701: Plant protein of unknown function (DUF827)
Length = 186
Score = 27.5 bits (58), Expect = 4.3
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = +2
Query: 38 KITMAKPKGERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTP 217
K + K + E K S + E +T+E K H + P+ +++ E + P
Sbjct: 53 KENLKKAEEENKVLSQLIESLTQELETTKEKLNHSLRNFPEHPQVEDDLKFIEESTVNEP 112
Query: 218 DIRVDTRLNKF 250
D + ++N+F
Sbjct: 113 DNITEIKMNRF 123
>At1g32900.1 68414.m04053 starch synthase, putative similar to
starch synthase SP:Q42857 from [Ipomoea batatas]
Length = 610
Score = 27.1 bits (57), Expect = 5.7
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = +2
Query: 65 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRV 229
E+KG + E +++ +N+ + G G KK + ++ KF K +G V
Sbjct: 412 EQKGSDILVEAISKFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNV 466
>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 376
Score = 26.2 bits (55), Expect = 10.0
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 92 EVVT--REYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 250
EV+T ++ V K +G+G K+R + K+ K E+ P+ + LNKF
Sbjct: 36 EVLTMYEQWLVENGKNYNGLGEKERRFKIFKDNLKRIEEHNSDPNRSYERGLNKF 90
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 26.2 bits (55), Expect = 10.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 302 KASHGHGREHF*LP*TKGICLSE 234
+A G H+ +P T+G+CLSE
Sbjct: 342 EAREGEKNGHYHMPETRGLCLSE 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,232,203
Number of Sequences: 28952
Number of extensions: 172379
Number of successful extensions: 342
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 335
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 342
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -