BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8a08
(311 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putativ... 33 0.053
At5g54900.1 68418.m06838 RNA-binding protein 45 (RBP45), putativ... 31 0.12
At1g14670.1 68414.m01744 endomembrane protein 70, putative simil... 29 0.50
At2g01970.1 68415.m00132 endomembrane protein 70, putative 29 0.66
At5g37310.1 68418.m04481 endomembrane protein 70, putative multi... 29 0.87
At4g13430.1 68417.m02096 aconitase family protein / aconitate hy... 27 2.6
At1g52290.1 68414.m05900 protein kinase family protein contains ... 27 3.5
At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase fa... 26 4.6
At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase fa... 26 4.6
At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase fa... 26 4.6
At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative... 26 6.1
At5g07290.1 68418.m00832 RNA recognition motif (RRM)-containing ... 25 8.1
>At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putative
DNA binding protein ACBF - Nicotiana tabacum,
PID:g1899188
Length = 415
Score = 32.7 bits (71), Expect = 0.053
Identities = 15/47 (31%), Positives = 27/47 (57%)
Frame = -2
Query: 142 QEIAENIVKPCFEQFGTILHIYVCPLNHNRIIVEYANSESVQKAMTV 2
Q + E+ +K F QFG ++H+ + P V+YAN ++A++V
Sbjct: 287 QSVTEDDLKSVFGQFGELVHVKI-PAGKRCGFVQYANRACAEQALSV 332
>At5g54900.1 68418.m06838 RNA-binding protein 45 (RBP45), putative
contains similarity to polyadenylate-binding protein 5
Length = 387
Score = 31.5 bits (68), Expect = 0.12
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -2
Query: 136 IAENIVKPCFEQFGTILHIYVCPLNHNRIIVEYANSESVQKAMTV 2
+ ++ +K F QFG +LH+ + P V+YAN S + A++V
Sbjct: 271 VTDDELKSIFGQFGELLHVKI-PPGKRCGFVQYANKASAEHALSV 314
>At1g14670.1 68414.m01744 endomembrane protein 70, putative similar
to endomembrane protein emp70 precursor isolog
GB:AAF67014 GI:7677068 (Homo sapiens)
Length = 592
Score = 29.5 bits (63), Expect = 0.50
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +3
Query: 93 VPNCSKQGFTMFSAISCLVIFCSSSEWGFQSI*ILFIVVGRFTIIVLVFCLIS 251
+P + GF FSAI + + +S WG + I I+ F I+++V I+
Sbjct: 450 IPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILIIVTAFIT 502
>At2g01970.1 68415.m00132 endomembrane protein 70, putative
Length = 592
Score = 29.1 bits (62), Expect = 0.66
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = +3
Query: 93 VPNCSKQGFTMFSAISCLVIFCSSSEWGFQSI*ILFIVVGRFTIIVLVFCLIS 251
VP + GF FSAI + + +S WG + I I+ F I+++V I+
Sbjct: 450 VPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFIT 502
>At5g37310.1 68418.m04481 endomembrane protein 70, putative
multispanning membrane protein, Homo sapiens,
EMBL:HSU94831
Length = 564
Score = 28.7 bits (61), Expect = 0.87
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = +3
Query: 93 VPNCSKQGFTMFSAISCLVIFCSSSEWGFQSI*ILFIVVGRFTIIVLVFCLIS 251
+P + GF FSAI + + +S WG + I I+ F I+V+V I+
Sbjct: 451 LPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILSIVFLILVIVTAFIT 503
>At4g13430.1 68417.m02096 aconitase family protein / aconitate
hydratase family protein contains Pfam profile PF00330:
Aconitase family (aconitate hydratase
Length = 509
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = -1
Query: 131 RKHCKALF*TIWHHITHIRMSFKSQPNYCRVCKLRVSTKSH 9
R+HC+ ++ IT + +FK+ P+Y VC + ++ + H
Sbjct: 149 REHCREQNIKYFYDITDLG-NFKANPDYKGVCHVALAQEGH 188
>At1g52290.1 68414.m05900 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 509
Score = 26.6 bits (56), Expect = 3.5
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = -2
Query: 304 FVVSYQFIFDRVQKLYNREIKQKTKTIIVKRPTTMNRIQIDWKPHSEDEQKMTKQEIAEN 125
FV+ IF K R++K+K K I ++NR +D K S + Q+ + EI +N
Sbjct: 73 FVLLGVCIFVCFYKRKKRKLKKKKKEDI---EASINRDSLDPKDDSNNLQQWSSSEIGQN 129
Query: 124 I 122
+
Sbjct: 130 L 130
>At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 431
Score = 26.2 bits (55), Expect = 4.6
Identities = 17/72 (23%), Positives = 32/72 (44%), Gaps = 3/72 (4%)
Frame = -2
Query: 214 RPTTMNRIQIDWKPHSEDEQKMT---KQEIAENIVKPCFEQFGTILHIYVCPLNHNRIIV 44
RPT + I +P+S + + N + F +FG + +Y + R+IV
Sbjct: 93 RPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADDSGVRVIV 152
Query: 43 EYANSESVQKAM 8
+A+ S + A+
Sbjct: 153 SFADPFSAKAAL 164
>At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 344
Score = 26.2 bits (55), Expect = 4.6
Identities = 17/72 (23%), Positives = 32/72 (44%), Gaps = 3/72 (4%)
Frame = -2
Query: 214 RPTTMNRIQIDWKPHSEDEQKMT---KQEIAENIVKPCFEQFGTILHIYVCPLNHNRIIV 44
RPT + I +P+S + + N + F +FG + +Y + R+IV
Sbjct: 8 RPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADDSGVRVIV 67
Query: 43 EYANSESVQKAM 8
+A+ S + A+
Sbjct: 68 SFADPFSAKAAL 79
>At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 431
Score = 26.2 bits (55), Expect = 4.6
Identities = 17/72 (23%), Positives = 32/72 (44%), Gaps = 3/72 (4%)
Frame = -2
Query: 214 RPTTMNRIQIDWKPHSEDEQKMT---KQEIAENIVKPCFEQFGTILHIYVCPLNHNRIIV 44
RPT + I +P+S + + N + F +FG + +Y + R+IV
Sbjct: 93 RPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADDSGVRVIV 152
Query: 43 EYANSESVQKAM 8
+A+ S + A+
Sbjct: 153 SFADPFSAKAAL 164
>At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative
strong similarity to gi:4467359
Length = 1116
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = -2
Query: 268 QKLYNREIKQKTKTIIVKRPTTMNRIQIDWKPHSEDEQKMTKQEI 134
QKL++ + T+ ++ PT+ + +Q D S D+ K+ K+ I
Sbjct: 176 QKLFSLTLSPPTQKSLLFSPTSGSNLQDDGSQLSADDNKIFKRLI 220
>At5g07290.1 68418.m00832 RNA recognition motif (RRM)-containing
protein Mei2-like protein - Arabidopsis thaliana,
EMBL:D86122
Length = 907
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -2
Query: 109 FEQFGTILHIYVCPLNHNRIIVEYANSESVQKA 11
F+QFG + ++ N I+V Y + + QKA
Sbjct: 231 FKQFGDVRALHTAGKNRGFIMVSYYDIRAAQKA 263
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,088,172
Number of Sequences: 28952
Number of extensions: 102228
Number of successful extensions: 333
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 333
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 331449360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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