Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc8a01
(732 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58230.1 68418.m07290 WD-40 repeat protein (MSI1) contains 6 ... 279 1e-75
At4g35050.1 68417.m04974 WD-40 repeat protein (MSI3) contains 5 ... 174 4e-44
At2g16780.1 68415.m01924 WD-40 repeat protein (MSI2) contains 5 ... 171 3e-43
At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5 ... 84 1e-16
At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 ... 83 2e-16
At2g19540.1 68415.m02283 transducin family protein / WD-40 repea... 44 8e-05
At2g23170.1 68415.m02768 auxin-responsive GH3 family protein sim... 30 1.4
At4g29230.1 68417.m04181 no apical meristem (NAM) family protein... 29 2.4
At4g00590.1 68417.m00083 asparaginase 2 family protein very low ... 28 7.3
At3g24340.1 68416.m03056 SNF2 domain-containing protein / helica... 28 7.3
At3g49890.1 68416.m05454 expressed protein lea32, Arabidopsis th... 27 9.7
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 27 9.7
>At5g58230.1 68418.m07290 WD-40 repeat protein (MSI1) contains 6
WD-40 repeats (PF0400); identical to WD-40 repeat
protein (SP:O22467) [Arabidopsis thaliana]
Length = 424
Score = 279 bits (685), Expect = 1e-75
Identities = 122/184 (66%), Positives = 149/184 (80%), Gaps = 1/184 (0%)
Frame = +1
Query: 127 DGETFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPDGKDYSV 306
D E +EER+INEEYKIWKKNTPFLYDLV+THALEWPSLT +WLPD P GKDYSV
Sbjct: 4 DEEEMRGEIEERLINEEYKIWKKNTPFLYDLVITHALEWPSLTVEWLPDREEPSGKDYSV 63
Query: 307 HRLILGTHTSD-EQNHLLIASVQLPNEDAQFDASHYDNDKGEFGGFGSVSGKIDIEIKIN 483
++ILGTHTS+ E N+L++A VQLP +D + +A YD+D+ EFGGFG +GK+ I +IN
Sbjct: 64 QKMILGTHTSESEPNYLMLAQVQLPLDDTESEARQYDDDRSEFGGFGCATGKVQIIQQIN 123
Query: 484 HEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTKHPSKPEPSGECHPDLRLRGHQKEGYG 663
H+GEVNRARYMPQNP +IATKT +++V VFDY+KHPSKP G C+PDL+LRGH EGYG
Sbjct: 124 HDGEVNRARYMPQNPFIIATKTVNAEVYVFDYSKHPSKPPLDGACNPDLKLRGHSSEGYG 183
Query: 664 LSWN 675
LSW+
Sbjct: 184 LSWS 187
Score = 31.1 bits (67), Expect = 0.79
Identities = 23/79 (29%), Positives = 34/79 (43%)
Frame = +1
Query: 445 SVSGKIDIEIKINHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTKHPSKPEPSGECHP 624
S S ++ + H EVN + P N V+AT + V +FD K S H
Sbjct: 259 SPSASKPVQSVVAHSMEVNCLAFNPFNEWVVATGSTDKTVKLFDLRK------LSTALH- 311
Query: 625 DLRLRGHQKEGYGLSWNPK 681
H++E + + WNPK
Sbjct: 312 --TFDSHKEEVFQVGWNPK 328
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/75 (24%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
Frame = +1
Query: 481 NHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTKHPSKP--EPSGECHPDLRL--RGHQ 648
+H+ EV + + P+N ++A+ ++V+D ++ + E + + P+L GH
Sbjct: 315 SHKEEVFQVGWNPKNETILASCCLGRRLMVWDLSRIDEEQTVEDAEDGPPELLFIHGGHT 374
Query: 649 KEGYGLSWNPKP*WI 693
+ SWNP W+
Sbjct: 375 SKISDFSWNPCEDWV 389
>At4g35050.1 68417.m04974 WD-40 repeat protein (MSI3) contains 5
WD-40 repeats (PF0400); identical to WD-40 repeat
protein MSI3 (SP:O22469) [Arabidopsis thaliana]
Length = 424
Score = 174 bits (424), Expect = 4e-44
Identities = 84/173 (48%), Positives = 115/173 (66%), Gaps = 3/173 (1%)
Frame = +1
Query: 166 INEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPDGKD--YSVHRLILGTHTS- 336
+ EE+ IWK+NTPFLYDL+++H LEWPSLT W+P P KD ++VH+LILGTHTS
Sbjct: 15 VEEEFSIWKRNTPFLYDLMISHPLEWPSLTLHWVPSTPIPYSKDPYFAVHKLILGTHTSG 74
Query: 337 DEQNHLLIASVQLPNEDAQFDASHYDNDKGEFGGFGSVSGKIDIEIKINHEGEVNRARYM 516
Q+ L++A V +P DA+ D + + K++I+ KI +GEVNRAR M
Sbjct: 75 GAQDFLMVADVVIPTPDAEPGLGGRDQE--------PIVPKVEIKQKIRVDGEVNRARCM 126
Query: 517 PQNPCVIATKTPSSDVLVFDYTKHPSKPEPSGECHPDLRLRGHQKEGYGLSWN 675
PQ P ++ KT S+V +FDY + KP+ S EC PDLRL GH++EGYGL+W+
Sbjct: 127 PQKPTLVGAKTSGSEVFLFDYARLSGKPQTS-ECDPDLRLMGHEQEGYGLAWS 178
Score = 31.1 bits (67), Expect = 0.79
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +1
Query: 469 EIKINHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTK 585
++K+ HE E+N + P N V+AT + S V +FD K
Sbjct: 256 QVKV-HEREINYLSFNPFNEWVLATASSDSTVALFDLRK 293
>At2g16780.1 68415.m01924 WD-40 repeat protein (MSI2) contains 5
WD-40 repeats (PF0400); identical to WD-40 repeat
protein MSI2 (SP:O22468) [Arabidopsis thaliana] WD-40
repeats (PF0400);
Length = 415
Score = 171 bits (417), Expect = 3e-43
Identities = 90/192 (46%), Positives = 117/192 (60%), Gaps = 3/192 (1%)
Frame = +1
Query: 112 MGDKGDGETFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPDG 291
M D+G ET VEE ++ +WKKNTPFLYDL+++H LEWPSLT W+P P
Sbjct: 1 MADEGKEETGMGQVEE-----DFSVWKKNTPFLYDLLISHPLEWPSLTVHWVPSTPNPYV 55
Query: 292 KD--YSVHRLILGTHTS-DEQNHLLIASVQLPNEDAQFDASHYDNDKGEFGGFGSVSGKI 462
D + VH+LILGTHTS Q+ L++A V P +A+ + D K+
Sbjct: 56 ADSYFGVHKLILGTHTSGSAQDFLMVADVVTPTPNAEPGIGGANQD--------PFIPKV 107
Query: 463 DIEIKINHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTKHPSKPEPSGECHPDLRLRG 642
+I +I +GEVNRAR MPQ P ++ KT +V +FDY KH +K + S EC PDLRL G
Sbjct: 108 EIRQRIRVDGEVNRARCMPQKPTLVGAKTSGCEVFLFDYAKHAAKSQTS-ECDPDLRLVG 166
Query: 643 HQKEGYGLSWNP 678
H KEGYGLSW+P
Sbjct: 167 HDKEGYGLSWSP 178
Score = 31.5 bits (68), Expect = 0.60
Identities = 16/39 (41%), Positives = 23/39 (58%)
Frame = +1
Query: 469 EIKINHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTK 585
++K+ HE EVN + P N V+AT + S V +FD K
Sbjct: 255 QVKV-HEREVNYLSFNPFNEWVLATASSDSTVALFDLRK 292
Score = 27.5 bits (58), Expect = 9.7
Identities = 19/82 (23%), Positives = 36/82 (43%), Gaps = 6/82 (7%)
Frame = +1
Query: 466 IEIKINHEGEVNRARYMPQNPCVIATKTPSSDVLVFDYTK---HPSKPEPSGECHPDLRL 636
+ + +HEGEV + + P + V+A+ ++V+D + + E E P L
Sbjct: 297 LHVMSSHEGEVFQVEWDPNHETVLASSGEDRRLMVWDLNRVGEEQLEIELDAEDGPPELL 356
Query: 637 ---RGHQKEGYGLSWNPKP*WI 693
GH+ + +WN W+
Sbjct: 357 FSHGGHKAKISDFAWNKNEPWV 378
>At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5
WD-40 repeats (PF0400); similar to WD-40 repeat protein
MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 496
Score = 83.8 bits (198), Expect = 1e-16
Identities = 55/171 (32%), Positives = 81/171 (47%), Gaps = 4/171 (2%)
Frame = +1
Query: 154 EERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPDGKDYSVHRLILGTHT 333
++ +++ Y WK P LYD + H L WPSL+ +W P + + K RL L T
Sbjct: 50 QKATVDDTYSQWKTLLPILYDSFVNHTLVWPSLSCRWGPQLEQAGSK---TQRLYLSEQT 106
Query: 334 SDE-QNHLLIASVQLPNEDA-QFDASHYDNDKGEFGGFGSVSGKIDIEIKINHEGEVNRA 507
+ N L+IA+ + N F N+K S + I H GEVNR
Sbjct: 107 NGSVPNTLVIANCETVNRQVISFSLPKLLNEKAH-------SPFVKKYKTIIHPGEVNRI 159
Query: 508 RYMPQNPCVIATKTPSSDVLVFDYTKHPSKPEPSG--ECHPDLRLRGHQKE 654
R +PQN ++AT T S D+L+++ P + G + PDL L GHQ +
Sbjct: 160 RELPQNSKIVATHTDSPDILIWNTETQPDRYAVLGAPDSRPDLLLIGHQDD 210
>At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 (4
significant) WD-40 repeats (PF0400); identical to WD-40
repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 507
Score = 83.0 bits (196), Expect = 2e-16
Identities = 57/164 (34%), Positives = 80/164 (48%), Gaps = 3/164 (1%)
Frame = +1
Query: 166 INEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPDGKDYSVHRLILGTHTSDE- 342
++E+Y WK P LYD + H L WPSL+ +W P + + K+ RL L T
Sbjct: 64 VDEKYSQWKGLVPILYDWLANHNLVWPSLSCRWGPQLEQATYKNRQ--RLYLSEQTDGSV 121
Query: 343 QNHLLIASVQLPNEDAQFDASHYDNDKGEFGGFGSVSGKIDIEIKINHEGEVNRARYMPQ 522
N L+IA+ ++ A H E + S + I H GEVNR R +PQ
Sbjct: 122 PNTLVIANCEVVKPRVAA-AEHISQFNEE-----ARSPFVKKYKTIIHPGEVNRIRELPQ 175
Query: 523 NPCVIATKTPSSDVLVFDYTKHPSKPEPSGECH--PDLRLRGHQ 648
N ++AT T S DVL++D P++ G + PDL L GHQ
Sbjct: 176 NSKIVATHTDSPDVLIWDVETQPNRHAVLGAANSRPDLILTGHQ 219
>At2g19540.1 68415.m02283 transducin family protein / WD-40 repeat
family protein contains WD-40 repeats (PF00400); similar
to Glutamate-rich WD repeat protein (GRWD)
(SP:Q9BQ67)[Homo sapiens]
Length = 469
Score = 44.4 bits (100), Expect = 8e-05
Identities = 34/111 (30%), Positives = 50/111 (45%), Gaps = 11/111 (9%)
Frame = +1
Query: 379 NEDAQFDASHYDNDKGEFGGFGSVSGKIDIEIKINHEGEVNRARYMPQNPCVIATKTPSS 558
+ED + D+ D+D E S + I + ++ H G VNR R MPQN + + S
Sbjct: 128 DEDDEDDSDSDDDDGDE----ASKTPNIQVR-RVAHHGCVNRIRAMPQNSHICVSWADSG 182
Query: 559 DVLVFDYTKH-----PSKPEPSGECHPDL------RLRGHQKEGYGLSWNP 678
V V+D + H S+ E P L GH+ EGY + W+P
Sbjct: 183 HVQVWDMSSHLNALAESETEGKDGTSPVLNQAPLVNFSGHKDEGYAIDWSP 233
>At2g23170.1 68415.m02768 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 595
Score = 30.3 bits (65), Expect = 1.4
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -1
Query: 360 DQQVILFIRCVCS*DKSVYGIILSVWP 280
DQ++ FI VC D S GII +WP
Sbjct: 278 DQELADFITSVCGQDNSWEGIITKIWP 304
>At4g29230.1 68417.m04181 no apical meristem (NAM) family protein
similar to NAM family proteins GP|12751304|,
GP|6223650|, GP|9758909 - Arabidopsis
thaliana,PIR2:T04621
Length = 498
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/55 (21%), Positives = 28/55 (50%)
Frame = +1
Query: 280 RPDGKDYSVHRLILGTHTSDEQNHLLIASVQLPNEDAQFDASHYDNDKGEFGGFG 444
+P+ ++ +H+ LGTH +++ L+++ + + Q + S + GG G
Sbjct: 183 KPEKTNWVMHQYHLGTHEEEKEGELVVSKIFYQTQPRQCNWSSSTSSLNAIGGGG 237
>At4g00590.1 68417.m00083 asparaginase 2 family protein very low
similarity to glycosylasparaginase [SP|P20933] from Homo
sapiens; contains Pfam profile PF01112: Asparaginase 2
Length = 408
Score = 27.9 bits (59), Expect = 7.3
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 397 DASHYDNDKGEFGGFGSVSG 456
DAS D D G FGG G+V G
Sbjct: 88 DASLMDGDSGMFGGVGAVPG 107
>At3g24340.1 68416.m03056 SNF2 domain-containing protein / helicase
domain-containing protein similar to SP|P41410 DNA repair
protein rhp54 (RAD54 homolog) {Schizosaccharomyces
pombe}; contains PFam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain
Length = 1132
Score = 27.9 bits (59), Expect = 7.3
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 139 FDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEW 243
++ +VE + I+ ++I +K F+Y L++ EW
Sbjct: 1030 WNPSVESQAISRAFRIGQKRAVFIYHLMVKDTSEW 1064
>At3g49890.1 68416.m05454 expressed protein lea32, Arabidopsis
thaliana, EMBL:ATH131342
Length = 220
Score = 27.5 bits (58), Expect = 9.7
Identities = 15/56 (26%), Positives = 22/56 (39%)
Frame = +1
Query: 487 EGEVNRARYMPQNPCVIATKTPSSDVLVFDYTKHPSKPEPSGECHPDLRLRGHQKE 654
E VN P+N C + + +VFD+ P+ PD + G KE
Sbjct: 81 EDPVNIPEDKPENDCGVRLFKRCATGIVFDHVDEIRGPKKKPNLRPDKGVEGSSKE 136
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 27.5 bits (58), Expect = 9.7
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 303 GIILSVWPGDIGEPLGSERGPLQCMGH 223
G+ILS+ PGDIG+P + GH
Sbjct: 34 GLILSLVPGDIGKPTARSEKAIVNDGH 60
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,338,798
Number of Sequences: 28952
Number of extensions: 365218
Number of successful extensions: 936
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 925
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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