Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc7o15
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 283 1e-77
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 59 5e-10
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 40 5e-04
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 39 8e-04
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 39 8e-04
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 38 0.001
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 37 0.003
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 35 0.013
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 33 0.030
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.64
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 27 1.9
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 27 2.6
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 3.4
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 26 4.5
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 4.5
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 26 5.9
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 26 5.9
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 26 5.9
SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 25 7.9
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 283 bits (695), Expect = 1e-77
Identities = 136/171 (79%), Positives = 149/171 (87%)
Frame = +3
Query: 180 LDVTKLSALSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLG 359
LD+++LS + P +ISRQATINIGTIGHVAHGKSTVVKAISGV TVRFKNELERNITIKLG
Sbjct: 5 LDISELSPIHPAIISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLG 64
Query: 360 YANAKIYQCDNPKCPRPTSFISGGSSKDDSFPCLRPACTGRFQLVRHVSFVDCPGHDILM 539
YANAKIY+C N +CPRP + S S+K+D PC C LVRHVSFVDCPGHDILM
Sbjct: 65 YANAKIYKCSNEECPRPGCYRSYSSNKEDHPPC--EICNSPMNLVRHVSFVDCPGHDILM 122
Query: 540 ATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKID 692
ATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIM+LKHI+ILQNK+D
Sbjct: 123 ATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMQLKHIIILQNKVD 173
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 59.3 bits (137), Expect = 5e-10
Identities = 26/66 (39%), Positives = 40/66 (60%)
Frame = +3
Query: 495 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILI 674
RH + VDCPGH + M+ GAA MD A+++++ + PQT EHL + +K I++
Sbjct: 116 RHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDG-QMPQTREHLLLARQVGVKQIVV 174
Query: 675 LQNKID 692
NK+D
Sbjct: 175 YINKVD 180
Score = 36.3 bits (80), Expect = 0.004
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = +3
Query: 219 ISRQATINIGTIGHVAHGKSTVVKAIS 299
+ ++ +NIGTIGHV HGK+T+ AI+
Sbjct: 48 VRKKPHVNIGTIGHVDHGKTTLTAAIT 74
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 39.5 bits (88), Expect = 5e-04
Identities = 21/72 (29%), Positives = 35/72 (48%), Gaps = 6/72 (8%)
Frame = +3
Query: 495 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQP------QTSEHLAAIEIMK 656
R S +D PGH + M+NGA+ D +L+I+ + QT EH
Sbjct: 316 RRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQG 375
Query: 657 LKHILILQNKID 692
+ H++++ NK+D
Sbjct: 376 INHLVVVINKMD 387
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 38.7 bits (86), Expect = 8e-04
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +3
Query: 498 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMKL 659
+V+ +D PGH + M+ G + D A+L+I G QT EH + +
Sbjct: 86 NVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
Query: 660 KHILILQNKID 692
K +++ NK+D
Sbjct: 146 KQLIVAVNKMD 156
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 38.7 bits (86), Expect = 8e-04
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +3
Query: 498 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMKL 659
+V+ +D PGH + M+ G + D A+L+I G QT EH + +
Sbjct: 86 NVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
Query: 660 KHILILQNKID 692
K +++ NK+D
Sbjct: 146 KQLIVAVNKMD 156
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 38.3 bits (85), Expect = 0.001
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +3
Query: 498 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMKL 659
+V+ +D PGH + M+ G + D A+L+I G QT EH + +
Sbjct: 86 NVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
Query: 660 KHILILQNKID 692
K +++ NK+D
Sbjct: 146 KQLIVAVNKMD 156
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/51 (33%), Positives = 30/51 (58%)
Frame = +3
Query: 495 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIE 647
+ ++F+D PGH A GA + D +L++AG++ +PQT E + I+
Sbjct: 220 KFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDGV-KPQTVEAIKHIQ 269
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 34.7 bits (76), Expect = 0.013
Identities = 26/103 (25%), Positives = 52/103 (50%), Gaps = 1/103 (0%)
Frame = +3
Query: 144 TQSNLHQQDLSKLDVTKLSALSPEVISRQATINIGT-IGHVAHGKSTVVKAISGVQTVRF 320
T +++H Q L++ ++ K SA++ S ++ I A+GK+ ++ A Q + +
Sbjct: 825 TMTDVHDQKLAERELQKQSAIAANENSYRSLAEASPQIVFAANGKNGIIYA--NAQWLSY 882
Query: 321 KNELERNITIKLGYANAKIYQCDNPKCPRPTSFISGGSSKDDS 449
L ++ LG+ +A +Y D KC P S +++D+S
Sbjct: 883 SG-LSLESSLGLGFLSA-VYHADRKKCLLPESLEGTFNNQDES 923
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 33.5 bits (73), Expect = 0.030
Identities = 18/66 (27%), Positives = 34/66 (51%), Gaps = 6/66 (9%)
Frame = +3
Query: 513 DCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMKLKHILI 674
D PGH ++ M+ GA+ D A+L++ +++ QT EH + + + I++
Sbjct: 261 DAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVV 320
Query: 675 LQNKID 692
NK+D
Sbjct: 321 SVNKLD 326
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.1 bits (62), Expect = 0.64
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +3
Query: 510 VDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKI 689
+D PGH+ G ++ + A+L+I +PQT E + + K ++ L NK+
Sbjct: 554 IDTPGHESFTNLRSRGTSLCNIAILVIDIMHGL-EPQTIESIRLLRDQKTPFVVAL-NKV 611
Query: 690 D 692
D
Sbjct: 612 D 612
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +3
Query: 255 GHVAHGKSTVVKAISGVQT 311
GH++HG ST KAIS V T
Sbjct: 139 GHLSHGFSTPQKAISAVST 157
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +3
Query: 411 TSFISGGSSKDDSFPCLRPACTGRFQ 488
+ FI G + DD CL P C GR +
Sbjct: 18 SGFIKAGFAGDDIPKCLFPTCVGRIK 43
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.6 bits (56), Expect = 3.4
Identities = 17/58 (29%), Positives = 25/58 (43%)
Frame = +3
Query: 297 SGVQTVRFKNELERNITIKLGYANAKIYQCDNPKCPRPTSFISGGSSKDDSFPCLRPA 470
S QT + L + + +++A + P+PT F SGG SK F PA
Sbjct: 207 SNTQTANTTSNLR--VPVNASWSDAGRKEKSQENKPKPTPFGSGGPSKPTPFESHGPA 262
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +1
Query: 4 GCIRFCFCLTFTAVNENYYRIFFLKHFANSNKI 102
G + F FC +EN + +F F+NS+ I
Sbjct: 756 GSVDFVFCAGDDKTDENMFEVFLPTAFSNSHLI 788
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/53 (26%), Positives = 24/53 (45%)
Frame = +1
Query: 34 FTAVNENYYRIFFLKHFANSNKIGLTQPLWLRTKGELLNQTCISKTYLNWMSQ 192
F+ + RI FL +S K+G+T W+ + + IS NW ++
Sbjct: 1041 FSVLARKIVRIPFLDFSPSSFKLGITLWNWMMNQVPSFSSFLISNIIRNWKNE 1093
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 25.8 bits (54), Expect = 5.9
Identities = 20/63 (31%), Positives = 26/63 (41%)
Frame = +3
Query: 264 AHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYQCDNPKCPRPTSFISGGSSKD 443
A G V+ S Q F +E+ T+KL Y N + + P PR T S D
Sbjct: 396 ARGWCNVLDDESSQQNQNFSSEIGIFQTVKLWYLNESKFDTNPP--PRSTMSCRKLSGID 453
Query: 444 DSF 452
D F
Sbjct: 454 DPF 456
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/52 (28%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +3
Query: 204 LSPEVISRQATINIGTIGHVAHGKSTVVKAIS-GVQTVRFKNELERNITIKL 356
L P ++S++ T+N ++ H VVK + GV V++ L + +TI++
Sbjct: 2568 LYPSILSQEDTMNDNSLLPTFHSMVAVVKNDTYGVTYVKYATILLQELTIEI 2619
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = +3
Query: 495 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGN 599
+ ++ +D PGH + AV+D A+ +I G+
Sbjct: 93 QRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGS 127
>SPBC1539.09c |trp1||anthranilate synthase component
II|Schizosaccharomyces pombe|chr 2|||Manual
Length = 759
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 153 NLHQQDLSKLDVTKLSALSPEVISRQATINIGTIGHVAHGKSTVVKAI 296
NLH ++ +KL+ + P+ + A I + VAH S V A+
Sbjct: 452 NLHSFEVDLSTTSKLAEMVPDDVILAALSGISSPADVAHYSSQGVSAV 499
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,759,461
Number of Sequences: 5004
Number of extensions: 55462
Number of successful extensions: 179
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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