Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc7k24
(704 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52; cell... 48 3e-04
UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63; Euka... 47 5e-04
UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon nigrovirid... 39 0.14
UniRef50_Q7RA79 Cluster: Putative uncharacterized protein PY0662... 38 0.24
UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon nigrovirid... 36 0.73
UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondr... 35 1.7
UniRef50_A0CP61 Cluster: ER lumen protein retaining receptor; n=... 35 2.2
UniRef50_Q7RYR2 Cluster: Predicted protein; n=1; Neurospora cras... 35 2.2
UniRef50_Q5BXZ0 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 34 3.9
UniRef50_Q2ULD3 Cluster: Predicted protein; n=1; Aspergillus ory... 33 5.2
UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondri... 33 5.2
UniRef50_O57764 Cluster: Putative uncharacterized protein PH0016... 33 6.8
UniRef50_UPI000069DAAC Cluster: calcium activated chloride chann... 33 9.0
UniRef50_Q3WE82 Cluster: MMPL domain precursor; n=1; Frankia sp.... 33 9.0
>UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52;
cellular organisms|Rep: NADP-dependent malic enzyme -
Mus musculus (Mouse)
Length = 572
Score = 47.6 bits (108), Expect = 3e-04
Identities = 18/34 (52%), Positives = 23/34 (67%)
Frame = +3
Query: 369 ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWP 470
A+V P+P + EEF+ +QMYS Y LP YPWP
Sbjct: 527 ATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWP 560
>UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63;
Eukaryota|Rep: NADP-dependent malic enzyme - Homo
sapiens (Human)
Length = 572
Score = 46.8 bits (106), Expect = 5e-04
Identities = 17/40 (42%), Positives = 26/40 (65%)
Frame = +3
Query: 369 ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQPRV 488
A+V P+P + E F+R+QMYS +Y LP Y WP+ ++
Sbjct: 527 ATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKI 566
>UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 306
Score = 38.7 bits (86), Expect = 0.14
Identities = 15/40 (37%), Positives = 25/40 (62%)
Frame = +3
Query: 369 ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQPRV 488
A+++P+P D E ++RA +YS EY +Y WP++ V
Sbjct: 264 ATLRPEPSDKEAYVRALIYSTEYDEFAVDSYCWPEDSVTV 303
>UniRef50_Q7RA79 Cluster: Putative uncharacterized protein PY06623;
n=3; Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein PY06623 - Plasmodium yoelii yoelii
Length = 1155
Score = 37.9 bits (84), Expect = 0.24
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = -3
Query: 177 FHVNVLIYTKVVE*NLLNNSKFVMLNLEVYFVAVKGRTSIRNRDKRV 37
F+V +L+Y K + N LNN K ++N EV + +KG T++ K++
Sbjct: 452 FNVKILVYIKNIFYNFLNNEKNNLINNEVLYFLLKGITNLYELKKKI 498
>UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 694
Score = 36.3 bits (80), Expect = 0.73
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +3
Query: 369 ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQPRV 488
ASV P+P D E F+ + +YS Y + Y WP+ +V
Sbjct: 652 ASVYPEPKDKEAFVLSHIYSPNYDSFTLDMYDWPEEAMKV 691
>UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondrial
precursor; n=15; Bilateria|Rep: NADP-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 604
Score = 35.1 bits (77), Expect = 1.7
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = +3
Query: 369 ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQPRVAII 497
AS P+P D E F+R+ +Y+ +Y + +Y WP V +
Sbjct: 562 ASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV 604
>UniRef50_A0CP61 Cluster: ER lumen protein retaining receptor; n=2;
Paramecium tetraurelia|Rep: ER lumen protein retaining
receptor - Paramecium tetraurelia
Length = 280
Score = 34.7 bits (76), Expect = 2.2
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -1
Query: 350 CYGCVSCRFPLLIHHQ 303
C C+ CRFPLL+HHQ
Sbjct: 190 CLNCIICRFPLLLHHQ 205
>UniRef50_Q7RYR2 Cluster: Predicted protein; n=1; Neurospora
crassa|Rep: Predicted protein - Neurospora crassa
Length = 675
Score = 34.7 bits (76), Expect = 2.2
Identities = 23/72 (31%), Positives = 32/72 (44%)
Frame = +3
Query: 267 PDQDPMKQNVVVLMMDQQREPTGNAAVAEIVTGKASVQPQPLDTEEFIRAQMYSVEYGAA 446
P+ D + +N+ V M +P NAA E V G + +P EE + Q V A
Sbjct: 347 PEDDAVFKNLAVTNMAPGNKPKLNAAAPEFVAGDRAAKPSAKSNEEPVPKQNGDVVSEGA 406
Query: 447 LPPAYPWPDNQP 482
LP P +QP
Sbjct: 407 LPSPVRSP-SQP 417
>UniRef50_Q5BXZ0 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 166
Score = 33.9 bits (74), Expect = 3.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +3
Query: 363 GKASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWP 470
G + +PQP D E ++ +MY Y +P Y WP
Sbjct: 120 GLCNRKPQPEDIELYLSKKMYDPNYIPCMPEVYDWP 155
>UniRef50_Q2ULD3 Cluster: Predicted protein; n=1; Aspergillus
oryzae|Rep: Predicted protein - Aspergillus oryzae
Length = 237
Score = 33.5 bits (73), Expect = 5.2
Identities = 23/76 (30%), Positives = 40/76 (52%)
Frame = +3
Query: 267 PDQDPMKQNVVVLMMDQQREPTGNAAVAEIVTGKASVQPQPLDTEEFIRAQMYSVEYGAA 446
PD+DP K ++V LM +++R +A++ I+T SV Q T E I + +++G
Sbjct: 24 PDRDPAKLSMVELMQEKERIEEELSALSSILT---SVSNQMAPTLENISNHLNLLQHGVN 80
Query: 447 LPPAYPWPDNQPRVAI 494
+ + D+ PR I
Sbjct: 81 MNSSLTTFDDFPRADI 96
>UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=53; Eumetazoa|Rep: NAD-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 584
Score = 33.5 bits (73), Expect = 5.2
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +3
Query: 381 PQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQPRVAII 497
P+P D ++++ + + EY + LP Y WP++ +I
Sbjct: 544 PEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVI 582
>UniRef50_O57764 Cluster: Putative uncharacterized protein PH0016;
n=1; Pyrococcus horikoshii|Rep: Putative uncharacterized
protein PH0016 - Pyrococcus horikoshii
Length = 475
Score = 33.1 bits (72), Expect = 6.8
Identities = 23/67 (34%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = +3
Query: 279 PMKQNVVVLMMDQQREPTGNAAVA-EIVTGKASVQPQPLDTEEFIRAQMYSVEYGAALPP 455
P Q + + PT + A EIV K P PLD EFI Q+Y +EY L
Sbjct: 278 PSVQTYTIQSSFKYDHPTKDVVKAVEIVKSKKREAPDPLDIPEFI--QLYEIEYQKQLKI 335
Query: 456 AYPWPDN 476
P+ N
Sbjct: 336 LAPYIRN 342
>UniRef50_UPI000069DAAC Cluster: calcium activated chloride channel
1 precursor; n=1; Xenopus tropicalis|Rep: calcium
activated chloride channel 1 precursor - Xenopus
tropicalis
Length = 789
Score = 32.7 bits (71), Expect = 9.0
Identities = 27/110 (24%), Positives = 45/110 (40%), Gaps = 1/110 (0%)
Frame = +3
Query: 285 KQNVVVLMMDQQREPTGNAAVAEIVTGKASVQPQPLDTEEFIRAQMYSVEYGAALPPAYP 464
K VV L++D GN + + Q ++T +I +S YG L P
Sbjct: 305 KGRVVTLLIDVSGTMGGNNRIGRVFQAADIFLTQIIETGSYIGIVEFS-SYGFKLSDLKP 363
Query: 465 WPDNQPRVAI-I*PPHTTTHNRLVSKATLRTVCLVRVNVLDNVKVNETLY 611
N+ R + + PHTTT+ + LR V +N+L + + +
Sbjct: 364 IISNEDRENMKLLIPHTTTNRESNTCEGLRCALEVSLNILQQKPIQRSSF 413
>UniRef50_Q3WE82 Cluster: MMPL domain precursor; n=1; Frankia sp.
EAN1pec|Rep: MMPL domain precursor - Frankia sp. EAN1pec
Length = 745
Score = 32.7 bits (71), Expect = 9.0
Identities = 21/87 (24%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = +3
Query: 306 MMDQQRE-PTGNAAVAEIVTGK-ASVQPQPLDTEEFIRAQMYSVEYGAALPPAYPWPDNQ 479
+++ QR+ P G+A A +V + + LD +R ++ +P P PD++
Sbjct: 58 LLEAQRQLPGGDAVPAVVVLARDGGLTKADLDVATTLRTELAGFAATTTIPGVIPAPDHR 117
Query: 480 PRVAII*PPHTTTHNRLVSK-ATLRTV 557
+ + P TT +R + A +RT+
Sbjct: 118 AVIITVPMPQTTDADRFTADVARMRTI 144
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 581,785,519
Number of Sequences: 1657284
Number of extensions: 10196953
Number of successful extensions: 27096
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 26299
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27092
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 56198352344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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