Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc7d07
(717 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.04c |pgk1||phosphoglycerate kinase|Schizosaccharomyces ... 252 4e-68
SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces p... 29 0.66
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.5
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.5
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 27 2.7
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 27 3.5
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 4.7
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 26 4.7
SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 26 6.2
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 26 6.2
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 8.2
>SPBC14F5.04c |pgk1||phosphoglycerate kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 414
Score = 252 bits (617), Expect = 4e-68
Identities = 126/198 (63%), Positives = 147/198 (74%)
Frame = +3
Query: 123 KLSIDALNLTGKRVLMRVDFNVPLKEGVITNNQRIVAALDSVKYALDKGAKSVVLMSHLG 302
KL+I ++L GK VL+RVDFNVPL ITNN RIV AL ++KYAL++ K+V+LMSHLG
Sbjct: 6 KLAITDVDLKGKNVLIRVDFNVPLDGDRITNNARIVGALPTIKYALEQQPKAVILMSHLG 65
Query: 303 RPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHI 482
RP+G KY+LKPVA EL KLL K V FL+DC+GPEVE AC G +ILLENLRFHI
Sbjct: 66 RPNGARVAKYSLKPVAAELSKLLGKPVKFLDDCVGPEVEKACKEAKGGEVILLENLRFHI 125
Query: 483 EEEGKGVDASGAKVKADPEKVKAFRASLRKLGDVYINDAFGTAHRAHSSMVGEGFEQRAS 662
EEEG G KVKAD V+AFR SL LGD+++NDAFGTAHRAHSSMVG R S
Sbjct: 126 EEEG-SAKVDGKKVKADASAVEAFRKSLTSLGDIFVNDAFGTAHRAHSSMVGVDL-PRVS 183
Query: 663 GFLLKKELQYFAKALHEP 716
GFL+KKEL YF+KAL P
Sbjct: 184 GFLMKKELDYFSKALENP 201
>SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 598
Score = 29.1 bits (62), Expect = 0.66
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = +3
Query: 195 KEGVITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRPDGQVNLKYTLKPVAEELKKLLN 374
K V +AAL+ ++ KG K+VV++ G P G + LKP + + L
Sbjct: 443 KARVFDAENDFIAALERGEFK--KGEKTVVIIRFEG-PKGGPGMPEMLKPSSAIMGAGLG 499
Query: 375 KDVTFLND 398
KDV L D
Sbjct: 500 KDVALLTD 507
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 27.9 bits (59), Expect = 1.5
Identities = 32/116 (27%), Positives = 50/116 (43%), Gaps = 2/116 (1%)
Frame = -3
Query: 685 SSFFSKKPLALCSKPSPTIE--LCALCAVPKASLI*TSPSFLKLALKALTFSGSAFTLAP 512
SS S PL+ S SP + +L + +S S + LT S SA +
Sbjct: 58 SSSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSS 117
Query: 511 EASTPLPSSSMWKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSAT 344
+S L SSS SS + L+H+ S S+ + +S+ ++ SSSA+
Sbjct: 118 SSSHALSSSSSSLVASSSSSGMSSSSLSHSSSVPSSSSSYHS-SSMTTSGLSSSAS 172
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 27.9 bits (59), Expect = 1.5
Identities = 24/99 (24%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
Frame = -3
Query: 634 TIELCALCAVPKASLI*TSP-SFLKLALKAL-TFSGSAFTLAPEASTPLPSSSMWKRRFS 461
T++ C+ C + +SL + S +A + T + S++ + +STP SSS S
Sbjct: 107 TLQACSTCTISTSSLSYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPS 166
Query: 460 SRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSAT 344
S + + + S+S +S + +S + SSS++
Sbjct: 167 SSSSKSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSS 205
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 27.1 bits (57), Expect = 2.7
Identities = 21/67 (31%), Positives = 31/67 (46%)
Frame = -3
Query: 556 LKALTFSGSAFTLAPEASTPLPSSSMWKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTS 377
+K+ + +F + EA PSSS + F S + + STS + F+NVT
Sbjct: 516 IKSRNNNQMSFAMEEEADVSQPSSSSFTLSFPSALTSSKV----SSSTSHLLTRFRNVTL 571
Query: 376 LFSNFFS 356
L S FS
Sbjct: 572 LGSGEFS 578
>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1325
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/43 (23%), Positives = 20/43 (46%)
Frame = +3
Query: 354 ELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHI 482
E LL T + + P++ NP ++++L NL+ +
Sbjct: 145 ETDALLKNSATSIYKAVFPDLVQVLPNPEINNLVILRNLKLEV 187
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/63 (25%), Positives = 28/63 (44%)
Frame = -3
Query: 472 RRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATGLRVYLRFTWPSGLPKC 293
+ F S ++E + + VS + P +S N S +SSA+ + + P +PK
Sbjct: 176 QEFLSIVVENYKSMTTVVSEAFPPRSAPNTPSSHPMSAASSASPAEIGMEHAGPKMIPKA 235
Query: 292 DIS 284
S
Sbjct: 236 SSS 238
>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 968
Score = 26.2 bits (55), Expect = 4.7
Identities = 17/79 (21%), Positives = 34/79 (43%)
Frame = +3
Query: 177 DFNVPLKEGVITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRPDGQVNLKYTLKPVAEE 356
DF + + +N Q V+ +V +++ + +V ++HL D + + E
Sbjct: 285 DFPIDPFLSLESNVQTSVSQSSAVLKSINLAKQELVSVNHLVADDTKTPSPNLSSEIIEN 344
Query: 357 LKKLLNKDVTFLNDCIGPE 413
K + K + LN + PE
Sbjct: 345 TKADIKKSIRSLNKAVSPE 363
>SPCC306.04c |set1||histone lysine methyltransferase
Set1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/50 (26%), Positives = 23/50 (46%)
Frame = +3
Query: 357 LKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEEEGKGVD 506
LK L + + + +GPE T + ++ E LR+H + G+ D
Sbjct: 73 LKSLQTINYDYNENSLGPEPPTQVFVSNISPLVTSEQLRYHFKSFGEVFD 122
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.8 bits (54), Expect = 6.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 11 ILSISLHRNLKISRKLPKTESFEE 82
I + SLHR+ S K+ K SF+E
Sbjct: 137 ITNFSLHRSFSASGKMEKNNSFKE 160
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 25.4 bits (53), Expect = 8.2
Identities = 45/177 (25%), Positives = 78/177 (44%), Gaps = 6/177 (3%)
Frame = -3
Query: 685 SSFFSKKPLALCSKPSPTIELCALCAVPKASLI*TSPSFLKLALKALTFSGSAFTLAPEA 506
SS S L S S TI + + + +S+I PS L + + S+ +A +
Sbjct: 640 SSIISGSSSILSSSIS-TIPISSSLSTYSSSVI---PSSSTLVSSSSSLIVSSSPVASSS 695
Query: 505 STPLPSSSMWKRRFSSRI--IEPAEGLAHAVSTSGPMQSFKN----VTSLFSNFFSSSAT 344
S+P+PSSS +S+ + I + A+S+S + + N +T+ SN SS T
Sbjct: 696 SSPIPSSSSLVSTYSASLSNITHSSLSLTAMSSSSAIPTSVNSSTLITASSSNTLLSSIT 755
Query: 343 GLRVYLRFTWPSGLPKCDISTTDLAPLSKAYLTESKAATMRWLFVITPSLSGTLKST 173
+ T S + S T S++ LT S +T+ ++ S S T+ ++
Sbjct: 756 SSSAIVSSTTVSNISSNLPSAT---ASSQSQLTNS--STLATSLYLSSSSSRTISTS 807
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,756,699
Number of Sequences: 5004
Number of extensions: 53447
Number of successful extensions: 206
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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