Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc6e07
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 312 3e-86
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 176 2e-45
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 157 2e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 144 1e-35
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 31 0.11
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 29 0.75
SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 28 1.00
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 27 3.0
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 26 4.0
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 26 4.0
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 26 5.3
SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 26 5.3
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.3
SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 25 7.0
SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|c... 25 7.0
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 25 9.3
SPCC576.19c |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 25 9.3
SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.3
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 312 bits (766), Expect = 3e-86
Identities = 134/196 (68%), Positives = 168/196 (85%)
Frame = +2
Query: 53 MPSEMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHY 232
M E+ITLQ GQCGNQIG +FW+QLC+EHGI P+G LE FA+ G DRKDVFFYQ+DD Y
Sbjct: 1 MGREIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRY 60
Query: 233 IPRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASGYAQGEKLNEEVFD 412
IPRA+L+DLEPRV++ I++ Y LYNPEN+ ++K+GGGAGNNWA+GY+ E++ E++ D
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMD 120
Query: 413 IINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNLDEISD 592
+I+READGSD+LEGF L HSIAGGTGSG+GS++LE L+DR+PKK++QTYSVFPN +SD
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSD 180
Query: 593 VVVQPYNSLLTLKRLT 640
VVVQPYNSLL LKRLT
Sbjct: 181 VVVQPYNSLLALKRLT 196
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 176 bits (429), Expect = 2e-45
Identities = 80/195 (41%), Positives = 124/195 (63%), Gaps = 1/195 (0%)
Frame = +2
Query: 62 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHYIPR 241
E++ +Q GQCGNQ+G FW + EHG+ GI + A +R +V+F +A Y+PR
Sbjct: 3 EIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPR 62
Query: 242 AVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFDII 418
AVL+DLEP + + + + L+ P+N+ + GAGN WA G Y +G +L + V D++
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQ--SGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 419 NREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVV 598
REA+ D L+GF L HS+ GGTGSGMG+ +L + + +P +++ T+SV P + SD V
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAP-APKSSDTV 179
Query: 599 VQPYNSLLTLKRLTE 643
V+PYN+ L++ +L E
Sbjct: 180 VEPYNATLSMHQLVE 194
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 157 bits (380), Expect = 2e-39
Identities = 82/191 (42%), Positives = 115/191 (60%), Gaps = 3/191 (1%)
Frame = +2
Query: 62 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILE-EFASAGSDRK-DVFFYQADDDHYI 235
E+I++ +GQ G QIG W+ C+EHGI P G + E AS SD FF + Y+
Sbjct: 3 EIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYV 62
Query: 236 PRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFD 412
PR++ +DLEP VI + PY L++PE + K A NN+A G Y G++L +EV D
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKED--ASNNYARGHYTVGKELVDEVTD 120
Query: 413 IINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNLDEISD 592
I R AD L+GF++ HS GGTGSG G+ +LE L+ + KK +SV+P ++S
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP-APQVST 179
Query: 593 VVVQPYNSLLT 625
VV+PYNS+LT
Sbjct: 180 SVVEPYNSVLT 190
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 144 bits (348), Expect = 1e-35
Identities = 75/195 (38%), Positives = 114/195 (58%), Gaps = 7/195 (3%)
Frame = +2
Query: 62 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAG------SDRKDVFFYQADD 223
E+I++ +GQ G QIG W+ C+EHGI P+G E + +D FF +
Sbjct: 3 EVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQ 62
Query: 224 DHYIPRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNE 400
++PR++ +DLEP VI + PY L++PE + K A NN+A G Y G+++ +
Sbjct: 63 GKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKED--ASNNYARGHYTVGKEMID 120
Query: 401 EVFDIINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNLD 580
V + I R AD L+GF++ HS GGTGSG+G+ +LE L+ + KK +SV+P
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP-AP 179
Query: 581 EISDVVVQPYNSLLT 625
++S VV+PYNS+LT
Sbjct: 180 QVSTSVVEPYNSVLT 194
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 31.5 bits (68), Expect = 0.11
Identities = 19/63 (30%), Positives = 30/63 (47%)
Frame = +2
Query: 455 FVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKR 634
F L HSI G Y + +SD++ K+ + Y +F +L IS + + L L
Sbjct: 1370 FNLLHSIISYVRKHQGGYGVVAVSDKYSKQTLSRYPIFWSLVHISGLPNPDPSDLKLLNH 1429
Query: 635 LTE 643
LT+
Sbjct: 1430 LTD 1432
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 28.7 bits (61), Expect = 0.75
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 431 DGSDNLEGFVLCHSIAGGTGSG 496
DG + EGF + +IAGG G+G
Sbjct: 1104 DGENGEEGFAMYETIAGGAGAG 1125
>SPBC8D2.19 |mde3||serine/threonine protein kinase
Mde3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 559
Score = 28.3 bits (60), Expect = 1.00
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -3
Query: 269 HAAPNPAELLVVCSDHHQLDRKTHLYDQIP 180
++ PNPAE+ +C+DH + + L+ P
Sbjct: 530 YSTPNPAEVQNICNDHAKFETSKSLHLSSP 559
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 26.6 bits (56), Expect = 3.0
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 473 IAGGTGSGMGSYILEHLSDRFPKKLVQTYSV 565
+ G G+G +++ HL+ F KK + T V
Sbjct: 14 LVGDGGTGKTTFVKRHLTGEFEKKYIATLGV 44
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +2
Query: 74 LQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRK 196
LQ+ Q F W+ C++ G +P+ L+ F + D K
Sbjct: 18 LQICNMDEQTMFYKWESWCLQRGNTPKLDLDTFKAFAKDMK 58
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 26.2 bits (55), Expect = 4.0
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +2
Query: 143 ISP-EGILEEFASAGSDRKDVFFYQAD 220
ISP EGI+ E A+ D D+F Y D
Sbjct: 1029 ISPVEGIIPEDANVRFDNVDIFLYDCD 1055
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 236 PRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLS 334
P A ++ EP + SP+A ++NP NV S
Sbjct: 1588 PSAGSVEREPLFLIAASGSPHAFIWNPHNVSAS 1620
>SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase
Mde10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +2
Query: 29 YPELI*IKMPSEMITLQLGQCGNQI 103
Y L + PSE L+LG CGN I
Sbjct: 298 YVSLSCLSKPSEKSVLRLGTCGNGI 322
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.3
Identities = 17/74 (22%), Positives = 34/74 (45%)
Frame = +2
Query: 80 LGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHYIPRAVLLDL 259
LG G+ WK++ + ++ G++EEF + +D Y H + R + + L
Sbjct: 958 LGAFGDSSDIRDWKKISSQRRVTQLGVVEEF-DILLELRDKTLYA----HKLSRIIEMGL 1012
Query: 260 EPRVIHTIMNSPYA 301
I ++ +P+A
Sbjct: 1013 IESKIAVVIGTPHA 1026
>SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 570
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +2
Query: 122 QLCIEHGISPEGILEEFASAGSDRKDVFFYQAD 220
QL +E G S G+L + ++ D++F AD
Sbjct: 197 QLVMELGQSKNGVLGTVSKTANEGPDIYFSNAD 229
>SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 571
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2
Query: 467 HSIAGGTGSGMGSYILEHLSDRF 535
+ + GGT SG+GS L+ L+ RF
Sbjct: 345 NKVEGGTRSGIGSGFLKRLALRF 367
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1207
Score = 25.0 bits (52), Expect = 9.3
Identities = 8/28 (28%), Positives = 16/28 (57%)
Frame = +3
Query: 180 RDLIVKMCFSIKLMMITTYHEQFCWIWS 263
R L+ +C + + + T+H ++ WI S
Sbjct: 85 RKLLFSLCGHMDYVRVCTFHHEYPWILS 112
>SPCC576.19c |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 119
Score = 25.0 bits (52), Expect = 9.3
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 283 NRVYNTRLQIQQNCSWYVVIIISLIEKHIFTIRS 182
N +Y TRL +Q W++ I + L RS
Sbjct: 6 NDLYQTRLDVQTPNRWFLYISLELFNNMYSPFRS 39
>SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 25.0 bits (52), Expect = 9.3
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +2
Query: 437 SDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFPKKLVQTYSVFPNL 577
S +L V +IAG G GM S + +SD P K TY N+
Sbjct: 174 SRSLWQLVAARAIAGIGGGGMNSTVSILMSDIVPLKQRGTYQGIINV 220
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,708,550
Number of Sequences: 5004
Number of extensions: 58624
Number of successful extensions: 175
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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