Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc5l06
(673 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 168 7e-43
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 52 1e-07
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 51 1e-07
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 46 4e-06
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 39 8e-04
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 36 0.007
SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces p... 30 0.018
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 31 0.20
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.46
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 27 3.3
SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|c... 26 4.3
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.3
SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces po... 26 4.3
SPAC328.08c |||tubulin specific chaperone cofactor C |Schizosacc... 26 5.7
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 25 7.5
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 25 7.5
SPCC63.06 |||human WDR89 family WD repeat protein|Schizosaccharo... 25 7.5
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 25 7.5
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 9.9
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 9.9
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 168 bits (408), Expect = 7e-43
Identities = 89/205 (43%), Positives = 116/205 (56%), Gaps = 12/205 (5%)
Frame = +1
Query: 94 LDRKGTFKVEYLQEIEKKVQERWESQKIFEVEAPDDG-------KSHEKFLCTFPYPYMN 252
L + T K + L E+EKK Q++W+ +K FEV+AP + K + KF PYPYMN
Sbjct: 18 LKLENTTKRDTLIELEKKYQQKWQEEKAFEVDAPLEDVPIDELRKKYPKFFGNMPYPYMN 77
Query: 253 GRLHLGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKREMAVYGCPPV 432
G LHLGH F+LSK EF + RL G+ VLFP GFHCTGMPI A AD+L RE+ ++G
Sbjct: 78 GALHLGHAFTLSKVEFTTAFERLNGKRVLFPMGFHCTGMPICASADRLSREIEMFGPSFD 137
Query: 433 FPEDEELIVKDEDIIPXXXXXXXXXXXXXXXXXXX-----YQWQIMKSIGVPEEEIKEFA 597
PE++E V+ E P YQ+QIM+S+GVP EI +FA
Sbjct: 138 VPEEKEEEVEVEVKTPNAREDVTKHSGKKSKAAAKTAAVKYQFQIMESLGVPRTEIHKFA 197
Query: 598 NESYWLEYFPPRAVTDLKRMGIHVD 672
+ YWL YFPP D G+ +D
Sbjct: 198 DAKYWLSYFPPLCQRDCTEFGLGID 222
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 51.6 bits (118), Expect = 1e-07
Identities = 27/88 (30%), Positives = 45/88 (51%), Gaps = 2/88 (2%)
Frame = +1
Query: 136 IEKKVQERWESQKIFEVEAPDDGKSHEK--FLCTFPYPYMNGRLHLGHTFSLSKCEFACR 309
+E + W FE E DGK ++ F+ T P P + G LH+GH +++ + R
Sbjct: 102 VESAWYDWWVKSGFFEPEFGPDGKPKKEGVFVITSPPPNVTGALHIGHALTIAIQDSLAR 161
Query: 310 YYRLKGRMVLFPFGFHCTGMPIKACADK 393
+ R+ G+ VLF GF G+ ++ +K
Sbjct: 162 WNRMLGKTVLFLGGFDHAGLSTQSVVEK 189
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 51.2 bits (117), Expect = 1e-07
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +1
Query: 148 VQERWESQKIFEVEAPDDGKSHEKFLCTFPYPYMNGRLHLGHTFSLSKCEFACRYYRLKG 327
+ E W+S + K +K++ + +PY +G LH+GH + + RYYR+KG
Sbjct: 37 IAENWKSYWKSHYPFVKNDKGKKKYILSM-FPYPSGLLHIGHVRVYTISDILSRYYRMKG 95
Query: 328 RMVLFPFGFHCTGMPIKACA 387
V+ P G+ G+P + A
Sbjct: 96 YKVIHPMGWDAFGLPAENAA 115
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 46.4 bits (105), Expect = 4e-06
Identities = 22/55 (40%), Positives = 32/55 (58%)
Frame = +1
Query: 241 PYMNGRLHLGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKRE 405
P+ NGRLH+GH + + R+ LKGR V + G+ C G+PI++ A K E
Sbjct: 87 PFANGRLHIGHALNKILKDIINRWQLLKGRSVHYVPGWDCHGLPIESKAIKANAE 141
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 38.7 bits (86), Expect = 8e-04
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +1
Query: 220 FLCTFPYPYMNGRLHLGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLK 399
F P P + G+LH+GH +++ + R+Y + G V F G G+ ++ +K
Sbjct: 60 FPILLPPPNITGKLHIGHALTITIQDALARFYAMHGYKVSFRPGTDHAGIATQSVVEKYL 119
Query: 400 REMAVY 417
++ VY
Sbjct: 120 QKKGVY 125
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 35.5 bits (78), Expect = 0.007
Identities = 26/86 (30%), Positives = 38/86 (44%), Gaps = 1/86 (1%)
Frame = +1
Query: 139 EKKVQERWESQKIFEVEAP-DDGKSHEKFLCTFPYPYMNGRLHLGHTFSLSKCEFACRYY 315
E+K+ E W F + G+ F P P+ GR H GH + + + RY
Sbjct: 9 EEKIVEFWREIDAFHTQLKLSQGRPTYTFF-DGP-PFATGRPHHGHLLASTIKDSVTRYA 66
Query: 316 RLKGRMVLFPFGFHCTGMPIKACADK 393
LKG V FG+ G+P++ DK
Sbjct: 67 CLKGYHVERRFGWDTHGLPVEHEIDK 92
>SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 832
Score = 29.9 bits (64), Expect(2) = 0.018
Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 7/70 (10%)
Frame = +1
Query: 244 YMNGRLHLGHTFSLSKCEFACRYYRLKGRMVLFPFG-------FHCTGMPIKACADKLKR 402
Y++ R GH +YY R VLFPFG F + + +K C ++L+
Sbjct: 668 YLSFRSSRGHCVYGEDVFVGYKYYEAVEREVLFPFGYGLSYTTFELSNLYLKNCGERLRI 727
Query: 403 EMAVYGCPPV 432
++ + P+
Sbjct: 728 DLEISNTGPM 737
Score = 23.0 bits (47), Expect(2) = 0.018
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 538 YQWQIMKSIGVPEEEIKEFA 597
Y QI++S+ P +E+KEF+
Sbjct: 746 YISQIVRSVNRPVKELKEFS 765
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 30.7 bits (66), Expect = 0.20
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +1
Query: 208 SHEKFLCTFPYPYMNGRLHLGHTFSLSKCEFACRYYRLK 324
S + + T P Y+N HLGH +SL + R+ LK
Sbjct: 17 SKKPYFLTTPIFYVNAAPHLGHLYSLVLTDAIARFQNLK 55
>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 489
Score = 29.5 bits (63), Expect = 0.46
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = -3
Query: 212 CDFPSSGASTSKIFWLSHLS*TFFSIS*RYSTLNVPF 102
C P+S AS S I W SHL+ +FS+ R S NV F
Sbjct: 246 CLLPASFASQSTIAWQSHLNSYYFSLRTR-SLNNVLF 281
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1
Query: 541 QWQIMKSIGVPEEEIKEFANES 606
QWQ+M++ G EE EFA S
Sbjct: 25 QWQVMRNQGATEENNPEFAQIS 46
>SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 26.2 bits (55), Expect = 4.3
Identities = 14/57 (24%), Positives = 25/57 (43%)
Frame = -1
Query: 295 HIWIEKMYDPNASVHSCMDMEKYTKTSHVISHHQELQLQKSSGSPIFPELSSQFPED 125
H + + +P HS D+ + ++ HH+ + +S F EL +QF D
Sbjct: 228 HESLPALQNPPIQSHSETDLYADEELDSILMHHERPPIPESPRVEEFEELLNQFEGD 284
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 26.2 bits (55), Expect = 4.3
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -2
Query: 393 LISTSFNRHPSTVESKWKQHHSTFQP 316
L S S +RH + SK KQH QP
Sbjct: 695 LASLSASRHADAISSKSKQHTEIAQP 720
>SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 427
Score = 26.2 bits (55), Expect = 4.3
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = -2
Query: 603 FIGKFFDLLFRNTNTLHDLPLVL*TTSLGYSFAFLAFTFVFGYN 472
F G D N NTL D +L +S+ Y+ + TF++ N
Sbjct: 31 FSGLLRDFNLNNKNTLQDRYELLRKSSIRYTILQIGITFIYLQN 74
>SPAC328.08c |||tubulin specific chaperone cofactor C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 25.8 bits (54), Expect = 5.7
Identities = 11/37 (29%), Positives = 17/37 (45%)
Frame = +1
Query: 211 HEKFLCTFPYPYMNGRLHLGHTFSLSKCEFACRYYRL 321
H C F +P + G +HL + C +C +RL
Sbjct: 167 HNATKCNFTFPTIQGSIHLSDINDSTIC-VSCHQFRL 202
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.4 bits (53), Expect = 7.5
Identities = 23/78 (29%), Positives = 35/78 (44%)
Frame = -1
Query: 469 LRP*QLTPHLQEILEDNHIQPFLALAYQHKL*SASQYSGIQMETAPFYLSTGSTCRQTHI 290
L P LTP L + + H L L + + L Q S I+ E P +L + T I
Sbjct: 108 LLPLLLTPSLLQGPLNLHYADLLLL-HLYLLNCHEQGSLIE-EPRPLFLDPSWKEKVTSI 165
Query: 289 WIEKMYDPNASVHSCMDM 236
+M DP+ + SC+ +
Sbjct: 166 MSPQMVDPSKMISSCLSL 183
>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 7.5
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = -2
Query: 369 HPSTVESKWKQHHSTFQPVVPAGKLT 292
H S +WKQH + + P GK T
Sbjct: 233 HQSVNLKRWKQHPDQIEFIPPDGKFT 258
>SPCC63.06 |||human WDR89 family WD repeat
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 331
Score = 25.4 bits (53), Expect = 7.5
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = -2
Query: 363 STVESKWKQHHSTFQPVVPAGKLTFG*RKCMTQMQASIHVWIWKSTQKLL 214
S V S W Q + F + K F +T+ AS+ +W +S QKL+
Sbjct: 90 SAVRS-WTQQSTPFTCIALNKKNQFATGSELTRSLASVQLWDVRSEQKLI 138
>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
Sad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 25.4 bits (53), Expect = 7.5
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1
Query: 115 KVEYLQEIEKKVQERWESQKIFEVEAPDDGK 207
KVE L++ K+ ++S FEVE DD K
Sbjct: 266 KVEQLKQSLKEEMSNYKSSVPFEVELNDDWK 296
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = -1
Query: 661 YPFSSNL*QHEVGSIQASSFHWQIL*SPLQE 569
+P S N E+G++ +SSF +Q++ S L +
Sbjct: 1498 FPRSMNGWLEEIGTMSSSSFEYQLVRSSLSD 1528
>SPAC1093.03 |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 25.0 bits (52), Expect = 9.9
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = -2
Query: 384 TSFNRHPSTVESKWKQHHSTFQPVVPAG 301
TS+ +H ++ +W Q S P P G
Sbjct: 283 TSYVQHRGSIPLRWSQEFSNITPKPPIG 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,918,907
Number of Sequences: 5004
Number of extensions: 62493
Number of successful extensions: 175
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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