Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc4n21
         (626 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce...    48   8e-07
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy...    40   3e-04
SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ...    35   0.011
SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha...    30   0.24 
SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch...    30   0.24 
SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar...    30   0.24 
SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha...    30   0.24 
SPAC607.03c |snu13||U3 snoRNP-associated protein Snu13|Schizosac...    29   0.73 
SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces po...    28   1.3  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    26   3.9  
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase...    25   6.8  
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr...    25   6.8  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    25   6.8  
SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyce...    25   9.0  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    25   9.0  
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone...    25   9.0  
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    25   9.0  

>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 285

 Score = 48.4 bits (110), Expect = 8e-07
 Identities = 26/88 (29%), Positives = 46/88 (52%)
 Frame = +3

Query: 84  DLVLADGSVDVNGRENEQERLNFDLIMCETQLILICLRPGGNCVLKVFDAFEHKTIQMLN 263
           DLV++DG+ DV G  +  E +   +++    L +  L+PGG  V K+F   +   +    
Sbjct: 119 DLVVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQL 178

Query: 264 KFVNHFEKWVLYKPPSSRPANSERYLIC 347
           + +  F K    KP SSR ++ E +++C
Sbjct: 179 RLM--FRKVSCAKPRSSRASSIESFVVC 204


>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 802

 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 26/88 (29%), Positives = 43/88 (48%)
 Frame = +3

Query: 84  DLVLADGSVDVNGRENEQERLNFDLIMCETQLILICLRPGGNCVLKVFDAFEHKTIQMLN 263
           D+VL DG+ +V     +       L++   +L    L  GG  V KVF + ++  +  + 
Sbjct: 113 DVVLHDGAPNVGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWV- 171

Query: 264 KFVNHFEKWVLYKPPSSRPANSERYLIC 347
            F   F K    KPPSSR  ++E +++C
Sbjct: 172 -FKQLFNKVEATKPPSSRNVSAEIFVVC 198


>SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 218

 Score = 34.7 bits (76), Expect = 0.011
 Identities = 25/99 (25%), Positives = 41/99 (41%)
 Frame = +3

Query: 60  SIKCGNACDLVLADGSVDVNGRENEQERLNFDLIMCETQLILICLRPGGNCVLKVFDAFE 239
           SI C    D V++D      G       L+ +L      + L  L+  G+ + K +   E
Sbjct: 121 SIDC-RVVDAVISDMLHKATGIRIRDHALSMELCASALHVALTFLKSNGSFICKFYMGDE 179

Query: 240 HKTIQMLNKFVNHFEKWVLYKPPSSRPANSERYLICFNK 356
              +Q L K  +HF    + KP +S   + E Y +C  +
Sbjct: 180 DADLQNLLK--SHFRFVQVMKPKASLKESREAYFVCLER 216


>SPBC1348.02 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 473 CVYKKPAASNQASFNMDLLIYDVDNYSFKNDSV 571
           CV  +P   + A FN+DL+ Y V      ND +
Sbjct: 61  CVAARPMTMDGAEFNLDLMGYSVSEDQINNDEI 93


>SPBPB2B2.19c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 473 CVYKKPAASNQASFNMDLLIYDVDNYSFKNDSV 571
           CV  +P   + A FN+DL+ Y V      ND +
Sbjct: 61  CVAARPMTMDGAEFNLDLMGYSVSEDQINNDEI 93


>SPAC977.01 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1||Partial|Manual
          Length = 316

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 473 CVYKKPAASNQASFNMDLLIYDVDNYSFKNDSV 571
           CV  +P   + A FN+DL+ Y V      ND +
Sbjct: 33  CVAARPMTMDGAEFNLDLMGYSVSEDQINNDEI 65


>SPAC750.05c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 344

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 473 CVYKKPAASNQASFNMDLLIYDVDNYSFKNDSV 571
           CV  +P   + A FN+DL+ Y V      ND +
Sbjct: 61  CVAARPMTMDGAEFNLDLMGYSVSEDQINNDEI 93


>SPAC607.03c |snu13||U3 snoRNP-associated protein
           Snu13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 125

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -2

Query: 157 KSKLRRSCSFSRPLTSTEPSANTKSHALPHLMLI 56
           K+ L R+C  SRP+ S   + N  S  LP +  I
Sbjct: 83  KAALGRACGVSRPVISASITTNEASDLLPQIQAI 116


>SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 635

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 15/36 (41%), Positives = 18/36 (50%)
 Frame = +1

Query: 223 FLTRSNTKRFKC*TSLLTILKNGFYTNRLLLGLPIP 330
           F+  S TK+    TSL   + NG Y  R  LGL  P
Sbjct: 359 FMFHSKTKKSSFNTSLGEFISNGIYEARKALGLLTP 394


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
 Frame = +3

Query: 93   LADGSVDVNGRENEQERLNFDLI-MCETQLILICLRPGGNCVLKVFDAFEHKTIQMLNKF 269
            LA+ +  +N R+ E ERL  D+I +    L +  L+   N   +     E K  ++  K 
Sbjct: 942  LAEANEKLNARDEEIERLKVDIIGLQNASLNMQSLKDSDN---RTISDLESKNKELEKKL 998

Query: 270  VNHFEKWVL 296
                E W+L
Sbjct: 999  KEADEYWLL 1007


>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
           Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1448

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = +3

Query: 291 VLYKPPSSRPANSERYLICFNKLVRPYCNDYVNELEKQFKK 413
           + Y PPSS   NS+ Y+  F   V     DY+   +++F +
Sbjct: 199 IRYSPPSSSKLNSKLYVKGFGTHVSLKRTDYLVVDDREFPR 239


>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
            heterochromatin assembly Hrr1|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1015

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +3

Query: 72   GNACDLVLADGSVDVNGRENEQERLNF 152
            G+   L   DG++ VNG++++Q + NF
Sbjct: 969  GDHLPLFTKDGTIYVNGKKSKQFQFNF 995


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
 Frame = -2

Query: 259 NI*IVLCSNASKTFKTQLPPGRKQIKISCVSHMIKSKLRRSCS---FSRPLTSTE---PS 98
           N+ I L +     FKT + P  KQI++S +    ++   R+ +    S  L + E   PS
Sbjct: 189 NLSITLYAWVGNAFKTHVFPQLKQIQVSDLEASFQNVTSRTTTGGHISNSLNTQEVVLPS 248

Query: 97  ANTKSHALPHL 65
            ++ +   PHL
Sbjct: 249 FSSNAKPKPHL 259


>SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 698

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/36 (33%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
 Frame = +3

Query: 300 KPPSSRPANSERYLICFNKLVRPYCND--YVNELEK 401
           +PP+ R  N +++   F+++  P+C D  Y+NE+ K
Sbjct: 542 RPPAKR-VNYQKFGDNFSEIGNPFCCDWVYLNEMVK 576


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 10/24 (41%), Positives = 15/24 (62%)
 Frame = -3

Query: 498 EAAGFLYTHVIFLTNLSTCLSFLI 427
           +A   +   V+F+T L TCL+F I
Sbjct: 113 QAVNIINIIVVFITTLLTCLAFAI 136


>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 809

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -2

Query: 184 KISCVSHMIKSKLRRSCSFSRPLTSTEPSANTKSHALPHLMLI 56
           + S  SH++K++     S S     T P A T+ +AL  LML+
Sbjct: 82  RFSDPSHLLKAREISVLSISEKCL-TSPRAGTRENALEALMLL 123


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 14/43 (32%), Positives = 22/43 (51%)
 Frame = -2

Query: 211 QLPPGRKQIKISCVSHMIKSKLRRSCSFSRPLTSTEPSANTKS 83
           +LPPG ++   S           R+ +++RP +ST P  NT S
Sbjct: 237 RLPPGWERRADSLGRTYYVDHNTRTTTWTRPASSTNPVHNTSS 279


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,336,781
Number of Sequences: 5004
Number of extensions: 44362
Number of successful extensions: 123
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)