Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc4e23
(711 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 64 7e-11
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 58 4e-09
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 58 4e-09
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 56 2e-08
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 49 4e-06
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 42 4e-04
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 39 0.004
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 35 0.061
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 35 0.061
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 34 0.11
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 33 0.14
At1g36180.1 68414.m04497 acetyl-CoA carboxylase 2 (ACC2) nearly ... 33 0.25
At1g36160.1 68414.m04495 acetyl-CoA carboxylase 1 (ACC1) nearly ... 33 0.25
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 30 1.3
At5g64380.1 68418.m08087 fructose-1,6-bisphosphatase family prot... 29 2.3
At5g56320.1 68418.m07029 expansin, putative (EXP14) similar to a... 29 4.0
At2g25180.1 68415.m03011 two-component responsive regulator fami... 29 4.0
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 28 7.0
At5g06800.1 68418.m00768 myb family transcription factor contain... 28 7.0
At4g00440.1 68417.m00061 expressed protein 27 9.3
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 64.5 bits (150), Expect = 7e-11
Identities = 30/73 (41%), Positives = 46/73 (63%), Gaps = 1/73 (1%)
Frame = +3
Query: 348 DVTTPSFPDSVSEGDVKLD-KKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKD 524
+ P +S+++G + KK GD V ADE + +IETDK I + +P GVI+E VK+
Sbjct: 95 EAVVPHMGESITDGTLAAFLKKPGDRVEADEAIAQIETDKVTIDIASPASGVIQEFLVKE 154
Query: 525 GETVKAGQKLFRL 563
G+TV+ G K+ R+
Sbjct: 155 GDTVEPGNKVARI 167
>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 463
Score = 58.4 bits (135), Expect = 4e-09
Identities = 27/70 (38%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +3
Query: 348 DVTTPSFPDSVSEGDVKLD-KKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKD 524
+ P +S+++G + KK G+ V ADE + +IETDK I + +P GVI+E V +
Sbjct: 93 EAVVPHMGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNE 152
Query: 525 GETVKAGQKL 554
G+TV+ G K+
Sbjct: 153 GDTVEPGTKV 162
>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 58.4 bits (135), Expect = 4e-09
Identities = 27/70 (38%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +3
Query: 348 DVTTPSFPDSVSEGDVKLD-KKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKD 524
+ P +S+++G + KK G+ V ADE + +IETDK I + +P GVI+E V +
Sbjct: 94 EAVVPHMGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNE 153
Query: 525 GETVKAGQKL 554
G+TV+ G K+
Sbjct: 154 GDTVEPGTKV 163
>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 365
Score = 56.0 bits (129), Expect = 2e-08
Identities = 26/62 (41%), Positives = 41/62 (66%), Gaps = 1/62 (1%)
Frame = +3
Query: 372 DSVSEGDVKLD-KKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKAGQ 548
+S+++G + KK G+ V ADE + +IETDK I + +P GVI+E V +G+TV+ G
Sbjct: 3 ESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVEPGT 62
Query: 549 KL 554
K+
Sbjct: 63 KV 64
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 48.8 bits (111), Expect = 4e-06
Identities = 31/111 (27%), Positives = 58/111 (52%), Gaps = 2/111 (1%)
Frame = +3
Query: 222 STTQTPKILAPLHATKLNQPRALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVSEGDV-K 398
STT T K+ +P+ KL + V ++ L Q++ PS +++EG++ +
Sbjct: 72 STTST-KLSSPMAGPKLFKEFISSQMRSVRGFSSSSDLPPHQEIGMPSLSPTMTEGNIAR 130
Query: 399 LDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDG-ETVKAGQ 548
KK GD VA EV+ E+ETDK + + G + ++ ++G + ++ G+
Sbjct: 131 WLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEIQVGE 181
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 41.9 bits (94), Expect = 4e-04
Identities = 21/70 (30%), Positives = 41/70 (58%), Gaps = 2/70 (2%)
Frame = +3
Query: 345 QDVTTPSFPDSVSEGDV-KLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVK 521
Q++ PS +++EG++ + KK GD VA EV+ E+ETDK + + G + ++
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKA 171
Query: 522 DG-ETVKAGQ 548
+G + ++ G+
Sbjct: 172 EGSKEIQVGE 181
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 38.7 bits (86), Expect = 0.004
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +3
Query: 360 PSFPDSVSEGDV-KLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDG 527
P+ ++S G+V K KK GD V +V+ EIETDK + + G + ++ V +G
Sbjct: 91 PALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKILVTEG 147
Score = 35.1 bits (77), Expect = 0.046
Identities = 16/57 (28%), Positives = 33/57 (57%), Gaps = 1/57 (1%)
Frame = +3
Query: 360 PSFPDSVSEGDV-KLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDG 527
P+ ++++G++ K KK GD + +V+ EIETDK + + G + ++ + +G
Sbjct: 218 PALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYLAKILIPEG 274
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 34.7 bits (76), Expect = 0.061
Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +3
Query: 348 DVTTPSFPDSVSEGDV-KLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKD 524
DV + ++E ++ K K GDSV + + E+++DK I + + G + +
Sbjct: 77 DVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSP 136
Query: 525 GETVKAGQKLFRLEI 569
G+ +K G+ L RL +
Sbjct: 137 GDIIKVGETLVRLAV 151
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 34.7 bits (76), Expect = 0.061
Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +3
Query: 348 DVTTPSFPDSVSEGDV-KLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKD 524
DV + ++E ++ K K GDSV + + E+++DK I + + G + +
Sbjct: 77 DVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSP 136
Query: 525 GETVKAGQKLFRLEI 569
G+ +K G+ L RL +
Sbjct: 137 GDIIKVGETLVRLAV 151
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 33.9 bits (74), Expect = 0.11
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +3
Query: 408 KVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKAGQKLF 557
KVGD V ++V IE K + A G I EL +DG+ V LF
Sbjct: 202 KVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVDTPLF 251
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 33.5 bits (73), Expect = 0.14
Identities = 19/68 (27%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +3
Query: 345 QDVTTPSFPDSVSEGD-VKLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVK 521
+++ P+ +++EG V K G+ +A E V+ +E+DK + V G + + V
Sbjct: 56 REIFMPALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVG 115
Query: 522 DGETVKAG 545
+GET G
Sbjct: 116 EGETAPVG 123
>At1g36180.1 68414.m04497 acetyl-CoA carboxylase 2 (ACC2) nearly
identical to acetyl-CoA carboxylase 2 (ACC2)
[Arabidopsis thaliana] GI:11869928
Length = 1755
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/50 (28%), Positives = 30/50 (60%)
Frame = +3
Query: 420 SVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKAGQKLFRLEI 569
S+ D E+E K +P+++P GVI + +G+ ++AG+ + +L++
Sbjct: 199 SIDTDTPYAEVEVMKMCMPLISPASGVI-HFKLSEGQAMQAGELIAKLDL 247
>At1g36160.1 68414.m04495 acetyl-CoA carboxylase 1 (ACC1) nearly
identical to acetyl-CoA carboxylase 1 (ACC1)
[Arabidopsis thaliana] GI:11869927
Length = 2247
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/50 (28%), Positives = 30/50 (60%)
Frame = +3
Query: 420 SVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKAGQKLFRLEI 569
++ AD E+E K +P+++P GVI + +G+ ++AG+ + L++
Sbjct: 697 NIDADTPYAEVEVMKMCMPLLSPASGVI-HFKMSEGQAMQAGELIANLDL 745
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 30.3 bits (65), Expect = 1.3
Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 1/77 (1%)
Frame = +3
Query: 345 QDVTTPSFPDSVSEGD-VKLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVK 521
+++ P+ +++EG V K GD + E V+ +E+DK + V G + + V+
Sbjct: 40 REIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVE 99
Query: 522 DGETVKAGQKLFRLEIT 572
+G G + L T
Sbjct: 100 EGGVAPVGSAIALLAET 116
>At5g64380.1 68418.m08087 fructose-1,6-bisphosphatase family protein
similar to SP|P22418 Fructose-1,6-bisphosphatase,
chloroplast precursor (EC 3.1.3.11)
(D-fructose-1,6-bisphosphate 1-phosphohydrolase)
(FBPase) {Spinacia oleracea}; contains Pfam profile
PF00316: fructose-1,6-bisphosphatase
Length = 404
Score = 29.5 bits (63), Expect = 2.3
Identities = 30/114 (26%), Positives = 55/114 (48%), Gaps = 4/114 (3%)
Frame = +3
Query: 171 KHIQTLYRRQGQSIRFKSTTQTPKILAPLHATKLNQPRALVAHNQVASIHFTNPL--LVE 344
K+I T+ + +GQ+ + S ++A LH T L A+ + + ++ NPL LVE
Sbjct: 291 KYIDTVRQGKGQNPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEGNPLAFLVE 350
Query: 345 Q--DVTTPSFPDSVSEGDVKLDKKVGDSVAADEVVLEIETDKTAIPVMAPGHGV 500
Q ++ +S VKL +++ + + E V E+E+ + PG+ V
Sbjct: 351 QAGGKSSDGKRGILSIQPVKLHQRLPLFLGSLEDVAELESYGDVQQTVNPGYEV 404
>At5g56320.1 68418.m07029 expansin, putative (EXP14) similar to
alpha-expansin 3 GI:6942322 from [Triphysaria
versicolor]; alpha-expansin gene family, PMID:11641069
Length = 255
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = +3
Query: 114 SVKIKGKYVQ*AKMLRRCSKHIQTLYRRQGQSIRFKSTTQTPKILAPLHATKLN 275
SV IKG + M R ++ Q+ + GQ++ FK TT + + +AT N
Sbjct: 186 SVSIKGTNTRWQSMSRNWGQNWQSNAKLDGQALSFKVTTSDGRTVISNNATPRN 239
>At2g25180.1 68415.m03011 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver domain
Length = 596
Score = 28.7 bits (61), Expect = 4.0
Identities = 19/72 (26%), Positives = 28/72 (38%)
Frame = +2
Query: 140 SISENATTLLEAHPDAVPASGPVHPLQVNNSNAQNTCTLTRNQTEPTESSGRPQSSSIHP 319
S N +LE HP A P P H + + N + + + P +S +S H
Sbjct: 403 SAPNNNVVVLEGHPQATPPGFPGHQINKRLEHWSNAVSSSTHPPPPAHNS----NSINHQ 458
Query: 320 LHESPACRARCD 355
SP +R D
Sbjct: 459 FDVSPLPHSRPD 470
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = +3
Query: 408 KVGDSVAADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKAGQKLF 557
KVGD V +V+ +E K + + G + ++ +DG+ V LF
Sbjct: 227 KVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPLF 276
>At5g06800.1 68418.m00768 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 375
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/36 (38%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = +2
Query: 257 TRNQTEPTE-SSGRPQSSSIHPLHESPACRARCDHS 361
T NQ+ P + SS +P SS HP H + D S
Sbjct: 59 TANQSFPVQCSSSKPYPSSFHPYHHQSSDSPSLDQS 94
>At4g00440.1 68417.m00061 expressed protein
Length = 831
Score = 27.5 bits (58), Expect = 9.3
Identities = 18/64 (28%), Positives = 35/64 (54%), Gaps = 1/64 (1%)
Frame = +3
Query: 237 PKILAPLHATKLNQPRALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVSEGDV-KLDKKV 413
P+ L+PL++ ++ AH + AS F N + ++++ T S P+ ++ V L K+
Sbjct: 431 PEYLSPLNSPGRRWEKSSTAHKKSASADFINLVNIKKE-THASQPEENTDIQVCNLSKEP 489
Query: 414 GDSV 425
DS+
Sbjct: 490 DDSI 493
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,716,716
Number of Sequences: 28952
Number of extensions: 293892
Number of successful extensions: 1049
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1022
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1049
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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