Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc4d19
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 148 7e-37
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 33 0.040
SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 33 0.053
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 29 0.49
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 29 0.86
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 27 2.0
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 2.0
SPAC24C9.09 |||mitochondrial threonine-tRNA ligase|Schizosacchar... 27 2.6
SPAC869.06c |||cation binding protein |Schizosaccharomyces pombe... 27 3.5
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 26 6.0
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 26 6.0
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 6.0
SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomy... 25 8.0
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 8.0
SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit... 25 8.0
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 148 bits (359), Expect = 7e-37
Identities = 77/200 (38%), Positives = 121/200 (60%), Gaps = 3/200 (1%)
Frame = +1
Query: 112 KFDLTFKIGQYLXRHXVFPLLEFLAAKETYDQSELLRAKLEILSKTNMIDYVTDIRRML- 288
K+DL+ KI Q+L RH +FPLLEFL+ ++T+D ELL+AK ++L TNM DYV ++ L
Sbjct: 14 KYDLSQKIMQHLDRHLIFPLLEFLSLRQTHDPKELLQAKYDLLKDTNMTDYVANLWTNLH 73
Query: 289 --YPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLS 462
+ +ED ++R VL EL EL++ V+ +L +++ D++ + +D + HL
Sbjct: 74 GGHTDEDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALR--QDKGQNLQHL- 130
Query: 463 TNKEYEFKIEMIDSMYRLAKYRYECGNYVESASYLYFCQLVMSPTDKNYLSVLWGKLASE 642
+ Y E I +Y+ A+++Y CGNY ++ LY + + N S WGK ASE
Sbjct: 131 -QEHYNITPERIAVLYKFAQFQYNCGNYGGASDLLYHFRAFSKDPELN-ASATWGKFASE 188
Query: 643 ILVQNWDGALDDLTKLREFI 702
IL +WDGA+++L KLRE +
Sbjct: 189 ILTVDWDGAMEELGKLREMV 208
>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 796
Score = 33.1 bits (72), Expect = 0.040
Identities = 22/61 (36%), Positives = 31/61 (50%)
Frame = -3
Query: 612 QIILVSWRHHQLTKVKI*C*LHIISTFIAIFGQTIHAIDHFNFKLILLVCRKMVYKSFRI 433
Q++ V W+HHQ C I + F IFGQ I AID ++ KL + + +SF
Sbjct: 332 QLLEVPWQHHQ-------CHPLIKTHFFGIFGQKIDAIDFYSAKLYKISQQIENARSFDY 384
Query: 432 P 430
P
Sbjct: 385 P 385
>SPBC1539.10 |||ribosome biogenesis protein Nop16
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 32.7 bits (71), Expect = 0.053
Identities = 25/87 (28%), Positives = 42/87 (48%), Gaps = 1/87 (1%)
Frame = +1
Query: 190 KETYDQSELLRAKLEILSKTNMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSELQELQDA 369
++ +D+ LR L +YVT LYP+ P+ + R + EL EL+ +
Sbjct: 37 QQNWDKHATLRQNYARLGLLATPNYVTGGVEKLYPD---PKRENEDRELTSEELDELKKS 93
Query: 370 VEPVLRLMQRDDVMKTIETMR-DPKTL 447
+ P +++RDD IE + + KTL
Sbjct: 94 LPPGQAIVRRDDDGNIIEIIHGEAKTL 120
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 29.5 bits (63), Expect = 0.49
Identities = 11/44 (25%), Positives = 25/44 (56%)
Frame = +1
Query: 415 TIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRLAKYRYECGNY 546
T+ T+ D ++ HL+ +F + ++M++ ++YRY N+
Sbjct: 180 TVATLGDLIGMVGHLNDTSGVDFNLNEYNAMWKTSEYRYVDFNF 223
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 28.7 bits (61), Expect = 0.86
Identities = 14/59 (23%), Positives = 29/59 (49%), Gaps = 5/59 (8%)
Frame = -3
Query: 537 TFIAIFGQTIHAIDHFNFKLILLVCRKMVYKSFRIPH-----GFNCFHYIISLHESQYW 376
TF+ +G I+ D +N L +VY ++ + F+ + ++S+++SQ W
Sbjct: 83 TFLVKYGDLINIQDPYNLNKTLFSVSDLVYNGIQLDYDSYSISFDAKYVLVSVNKSQRW 141
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 27.5 bits (58), Expect = 2.0
Identities = 16/63 (25%), Positives = 29/63 (46%)
Frame = +1
Query: 226 KLEILSKTNMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDD 405
K+ + S N I Y + +L P+E ++ RG+ + Q +++ E VL+
Sbjct: 188 KITLNSVVNSISYSSFFEELLITSYAKPKEALRTRGLAIVWNQRWKNSPESVLKARSEIT 247
Query: 406 VMK 414
V K
Sbjct: 248 VCK 250
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 27.5 bits (58), Expect = 2.0
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Frame = +1
Query: 400 DDVMKTIETMRDPKTLINHLSTNKE---YEFKIEMIDSMYRLAK 522
+DV+K + T+ PK +I L ++ +EF I+ ID ++ +K
Sbjct: 3764 NDVLKKLITILSPKVIITKLDFERDTFVHEFGIDSIDLIWLASK 3807
>SPAC24C9.09 |||mitochondrial threonine-tRNA
ligase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 473
Score = 27.1 bits (57), Expect = 2.6
Identities = 18/79 (22%), Positives = 37/79 (46%), Gaps = 5/79 (6%)
Frame = -3
Query: 441 FRIPHGFNCFHYIISLHESQYWLYC---ILKFLQFRKNY--TSPLYNFFRSVFFRIEHSS 277
F +PHG ++ ++ +QY ++ I+ L F+K+ S + + FR+E S
Sbjct: 69 FFLPHGTRIYNRLVDFLRAQYQIHGFEEIITPLIFKKDLWEKSGHWQNYEKEIFRVEESR 128
Query: 276 DVSNIIYHVSFAQNLQLCP 220
+V + + ++ CP
Sbjct: 129 NVEDEHSNATYGLKPMNCP 147
>SPAC869.06c |||cation binding protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 26.6 bits (56), Expect = 3.5
Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
Frame = -2
Query: 667 RHPNFVPKFR*PIYP-KEPTNNSCQLATSPVDKSKDMM 557
R FVP P P K P L +P+DK +DMM
Sbjct: 162 RTKKFVPTHSHPSAPNKPPFETVAGLFAAPIDKLRDMM 199
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = +1
Query: 373 EPVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIE 492
+PV+R+ + I + + P+ L H S K+Y++ ++
Sbjct: 1947 KPVIRISHFHHTFEVISSKQRPRRLTIHGSDGKDYQYVLK 1986
>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 781
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -2
Query: 601 CQLATSPVDKSKDMMLTPHNFHIHSDIW 518
C++ V KS D+ + H F ++SD W
Sbjct: 506 CRMEHPNVVKSYDLCIDSHIFPLYSDTW 533
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 25.8 bits (54), Expect = 6.0
Identities = 13/41 (31%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +1
Query: 265 VTDIRRMLYPEEDTPEEIIQRRGVVLSELQ-ELQDAVEPVL 384
VTDI+ +L + DTP ++ G + L+ +EP+L
Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTTPASVSLRQMIEPLL 573
>SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.4 bits (53), Expect = 8.0
Identities = 27/108 (25%), Positives = 49/108 (45%), Gaps = 1/108 (0%)
Frame = +1
Query: 193 ETYDQSELLRAKL-EILSKTNMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSELQELQDA 369
+T + + LR +L +I + +DYV RR+ E I G ++S Q D
Sbjct: 551 QTEELRKELRVQLPKIGGRLRDLDYVA--RRLRVNSSSVSEAI----GGIVS--QNASDI 602
Query: 370 VEPVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIEMIDSMYR 513
++ LR K T + TLI +LS ++ + + M+D +++
Sbjct: 603 LQTFLRPASLTSEEKPTFTPEESWTLITYLSQHEYIPYHMLMLDPLFK 650
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +1
Query: 127 FKIGQYLXRHXVFPLLEF-LAAKETYDQSELLRAKLEILSK 246
F I +YL + + P LEF L A E Q + A ++ SK
Sbjct: 1218 FHISKYLSSNLLLPFLEFLLTASEAEAQIPVTDASQKVSSK 1258
>SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit
Rpb4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 135
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 631 LASEILVQNWDGALDDLTKLREF 699
LA++I QN G LD+L+ LR+F
Sbjct: 111 LANKIDDQNLQGILDELSTLRKF 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,793,295
Number of Sequences: 5004
Number of extensions: 56405
Number of successful extensions: 176
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -