Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc3o08
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 116 1e-26
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 116 1e-26
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 113 1e-25
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 110 9e-25
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 110 9e-25
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 110 9e-25
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 102 2e-22
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 100 2e-21
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 100 2e-21
At3g23800.1 68416.m02991 selenium-binding family protein contain... 30 1.5
At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subuni... 30 1.5
At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subuni... 30 1.5
At4g20920.1 68417.m03032 double-stranded RNA-binding domain (DsR... 28 4.7
At3g03405.1 68416.m00338 hypothetical protein temporary automate... 28 6.2
At5g58360.1 68418.m07307 ovate family protein 69% similar to ova... 27 8.2
At3g21180.1 68416.m02677 calcium-transporting ATPase, plasma mem... 27 8.2
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 116 bits (279), Expect = 1e-26
Identities = 64/123 (52%), Positives = 84/123 (68%), Gaps = 2/123 (1%)
Frame = +3
Query: 282 GSRTDAEAVLREEEAISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIF 461
G TD++ V RE E++S K LR A+ + FQ EV+R+M +IINSLY NK+IF
Sbjct: 52 GLSTDSDVVHRESESMSK--------KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIF 103
Query: 462 LRELISNGSDALDKIRLMSLTDRGVLEA--NPELSIRIKAEPDKRLLHIIDSGVGMTRAD 635
LRELISN SDALDKIR ++LTD+ VL +L I+IK + K++L I D G+GMT+ D
Sbjct: 104 LRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKED 163
Query: 636 LIK 644
LIK
Sbjct: 164 LIK 166
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 116 bits (279), Expect = 1e-26
Identities = 64/123 (52%), Positives = 84/123 (68%), Gaps = 2/123 (1%)
Frame = +3
Query: 282 GSRTDAEAVLREEEAISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIF 461
G TD++ V RE E++S K LR A+ + FQ EV+R+M +IINSLY NK+IF
Sbjct: 52 GLSTDSDVVHRESESMSK--------KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIF 103
Query: 462 LRELISNGSDALDKIRLMSLTDRGVLEA--NPELSIRIKAEPDKRLLHIIDSGVGMTRAD 635
LRELISN SDALDKIR ++LTD+ VL +L I+IK + K++L I D G+GMT+ D
Sbjct: 104 LRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKED 163
Query: 636 LIK 644
LIK
Sbjct: 164 LIK 166
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 113 bits (272), Expect = 1e-25
Identities = 52/88 (59%), Positives = 67/88 (76%)
Frame = +3
Query: 378 AQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDRGVLEANPEL 557
A+ + FQ E+N+++ LIIN+ Y NKEIFLRELISN SDALDKIR SLTD+ L+ PEL
Sbjct: 9 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 68
Query: 558 SIRIKAEPDKRLLHIIDSGVGMTRADLI 641
IR+ + + L IIDSG+GMT+ADL+
Sbjct: 69 FIRLVPDKSNKTLSIIDSGIGMTKADLV 96
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 110 bits (264), Expect = 9e-25
Identities = 52/88 (59%), Positives = 65/88 (73%)
Frame = +3
Query: 378 AQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDRGVLEANPEL 557
A+ + FQ E+N+++ LIIN+ Y NKEIFLRELISN SDALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 558 SIRIKAEPDKRLLHIIDSGVGMTRADLI 641
I I + L IIDSG+GMT+ADL+
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLV 91
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 110 bits (264), Expect = 9e-25
Identities = 52/88 (59%), Positives = 65/88 (73%)
Frame = +3
Query: 378 AQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDRGVLEANPEL 557
A+ + FQ E+N+++ LIIN+ Y NKEIFLRELISN SDALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 558 SIRIKAEPDKRLLHIIDSGVGMTRADLI 641
I I + L IIDSG+GMT+ADL+
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLV 91
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 110 bits (264), Expect = 9e-25
Identities = 52/88 (59%), Positives = 65/88 (73%)
Frame = +3
Query: 378 AQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDRGVLEANPEL 557
A+ + FQ E+N+++ LIIN+ Y NKEIFLRELISN SDALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 558 SIRIKAEPDKRLLHIIDSGVGMTRADLI 641
I I + L IIDSG+GMT+ADL+
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLV 91
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 102 bits (245), Expect = 2e-22
Identities = 46/88 (52%), Positives = 68/88 (77%)
Frame = +3
Query: 378 AQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDRGVLEANPEL 557
A+ + +Q EV+R+M LI+NSLY NKE+FLRELISN SDALDK+R +S+T+ + + P+L
Sbjct: 94 AEKFEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRYLSVTNPELSKDAPDL 153
Query: 558 SIRIKAEPDKRLLHIIDSGVGMTRADLI 641
IRI A+ + ++ + DSG+GMTR +L+
Sbjct: 154 DIRIYADKENGIITLTDSGIGMTRQELV 181
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 99.5 bits (237), Expect = 2e-21
Identities = 46/106 (43%), Positives = 71/106 (66%)
Frame = +3
Query: 324 AISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDK 503
A+ DA + + + +Q EV+R++ LI++SLY +KE+FLREL+SN SDALDK
Sbjct: 58 AVKCDAAVAEKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDK 117
Query: 504 IRLMSLTDRGVLEANPELSIRIKAEPDKRLLHIIDSGVGMTRADLI 641
+R +S+T+ +L +L IRIK +PD + I D+G+GMT+ +LI
Sbjct: 118 LRFLSVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELI 163
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 99.5 bits (237), Expect = 2e-21
Identities = 46/106 (43%), Positives = 71/106 (66%)
Frame = +3
Query: 324 AISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDK 503
A+ DA + + + +Q EV+R++ LI++SLY +KE+FLREL+SN SDALDK
Sbjct: 58 AVKCDAAVAEKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDK 117
Query: 504 IRLMSLTDRGVLEANPELSIRIKAEPDKRLLHIIDSGVGMTRADLI 641
+R +S+T+ +L +L IRIK +PD + I D+G+GMT+ +LI
Sbjct: 118 LRFLSVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELI 163
>At3g23800.1 68416.m02991 selenium-binding family protein contains
Pfam profile: PF05694 56kDa selenium binding protein
(SBP56)
Length = 480
Score = 29.9 bits (64), Expect = 1.5
Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 1/80 (1%)
Frame = +3
Query: 348 VAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEI-FLRELISNGSDALDKIRLMSLT 524
V ++K R R FQ ++ + NSL+ + F EL+ GS L ++ T
Sbjct: 383 VPKIKGQRLRGGPQMFQLSLDGKRLYVTNSLFSVWDRQFYPELVEKGSHML---QIDVDT 439
Query: 525 DRGVLEANPELSIRIKAEPD 584
D+G L NP + EPD
Sbjct: 440 DKGGLSINPNFFVDFGTEPD 459
>At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 643
Score = 29.9 bits (64), Expect = 1.5
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 8/79 (10%)
Frame = +3
Query: 390 TFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDR---GVLEANPEL- 557
T + + ++ I Y +IF+ + S+ L KI + TD+ G++E EL
Sbjct: 401 TIKANIFQIFPWYIKVYYHTLQIFVDQQQKTDSEVLKKINVSPSTDKVSSGMMEMMLELP 460
Query: 558 ----SIRIKAEPDKRLLHI 602
S+ I E DK LHI
Sbjct: 461 CEVKSVAISIEYDKGFLHI 479
>At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 644
Score = 29.9 bits (64), Expect = 1.5
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 8/79 (10%)
Frame = +3
Query: 390 TFQTEVNRMMKLIINSLYRNKEIFLRELISNGSDALDKIRLMSLTDR---GVLEANPEL- 557
T + + ++ I Y +IF+ + S+ L KI + TD+ G++E EL
Sbjct: 401 TIKANIFQIFPWYIKVYYHTLQIFVDQQQKTDSEVLKKINVSPSTDKVSSGMMEMMLELP 460
Query: 558 ----SIRIKAEPDKRLLHI 602
S+ I E DK LHI
Sbjct: 461 CEVKSVAISIEYDKGFLHI 479
>At4g20920.1 68417.m03032 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 870
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/50 (38%), Positives = 28/50 (56%)
Frame = -3
Query: 202 ESSRTPSPKSQICFIAGVKNIQRDLDVNYSILNNCRKC*LTQVPSFNVLS 53
E +T +PK I GVK I R +V+ S + +C L Q+P F+V+S
Sbjct: 4 EEKQTLTPKEMILQKFGVKAIYRIEEVHVSSNDCLYRCHL-QLPEFSVVS 52
>At3g03405.1 68416.m00338 hypothetical protein temporary automated
functional assignment
Length = 193
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = -3
Query: 178 KSQICFIAGVKNIQRDLDVNYSILNNCRKC*LTQVPSFNVLSRDPLG 38
K +I +I G R+LD+ SI NC + VPS V + P G
Sbjct: 127 KHRISYILGENGYLRELDLGESIYTNCWTQACSYVPSL-VQLKQPAG 172
>At5g58360.1 68418.m07307 ovate family protein 69% similar to ovate
protein (GI:23429649) [Lycopersicon esculentum];
contains TIGRFAM TIGR01568 : uncharacterized
plant-specific domain TIGR01568
Length = 296
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/55 (36%), Positives = 27/55 (49%)
Frame = -3
Query: 610 ESMMCSSRLSGSALMRMLSSGFASSTPRSVSDIRRILSNASEPFEINSRRKISLF 446
+ M SSR + + LSS ASS+ + +RR+ S A P NS K S F
Sbjct: 25 KGMSRSSRKHHLSSPKHLSSADASSSRKLRDPLRRLSSTAHHPQASNSPPKSSSF 79
>At3g21180.1 68416.m02677 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA9)
identical to SP|Q9LU41 Potential calcium-transporting
ATPase 9, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 9) {Arabidopsis thaliana}
Length = 1086
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = -2
Query: 374 VAQLLHLRHAQRVRGDGLLLAQHGLGVSAGSFSG*TQV 261
V ++ LR +V DG+L++ H L + S +G +++
Sbjct: 290 VGDVIPLRIGDQVPADGVLISGHSLAIDESSMTGESKI 327
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,015,071
Number of Sequences: 28952
Number of extensions: 193749
Number of successful extensions: 623
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 621
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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