BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc3n15
(747 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 90 3e-19
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 27 2.8
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 25 8.7
>SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 90.2 bits (214), Expect = 3e-19
Identities = 55/165 (33%), Positives = 87/165 (52%), Gaps = 6/165 (3%)
Frame = +3
Query: 264 VVVLGDIGRSPRMQYHALSLANNGLDVKIISYVETDP---LPEVLNNPHITVIKLHPFEL 434
V+VLGDI RSPRMQYHA+S A G V ++ Y L E N I P L
Sbjct: 29 VLVLGDIARSPRMQYHAVSFAKLGWKVDLLGYQHPGSSVGLFESHENIRFYPIPSLPAYL 88
Query: 435 K-WGPVVLKYIA--KTLWQSVSLMFTLFLTGRCDYLLGQNPPAIPSLPVFRLYCMVSKAQ 605
+ + ++ K L Q ++L + LF+ +L QNPP IP + + ++ +
Sbjct: 89 QPKNRLQFLFLGPLKVLHQFLALNWALFVRKPASFLFIQNPPCIPVFFIAQCLHILRGTK 148
Query: 606 FVIDWHNYAYSIMAMTLEPDHMLVRMARSTERFFGQSSHFNLCVT 740
F+IDWHN+ YSI+A+ L H V++ + E++ + ++ +L V+
Sbjct: 149 FIIDWHNFGYSILALKLGKQHTFVKLLKIYEKYMARGAYAHLTVS 193
>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 27.1 bits (57), Expect = 2.8
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +3
Query: 309 HALSLANNGLDVKIISYVETDPLPEVLNNPHITVIKLHPF 428
H L++ LD I +E PLP V N HI +L F
Sbjct: 359 HLPLLSDEKLDKSAIDKIEPTPLPSVHMNSHIAKGELPKF 398
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = +3
Query: 447 VVLKYIAKTLWQSVSLMFTLFLTGRCDYLLGQNPPAIPSL 566
VV+ + L L + L L G+C+Y++ +PP+ L
Sbjct: 170 VVVTSFERDLIPFHELYYKLALEGKCNYVIRYSPPSSSKL 209
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,145,287
Number of Sequences: 5004
Number of extensions: 66865
Number of successful extensions: 126
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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