Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc3a02
         (637 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr...    53   3e-09
AY569709-1|AAS86662.1|  408|Apis mellifera complementary sex det...    23   3.3  
AY569704-1|AAS86657.1|  426|Apis mellifera complementary sex det...    22   4.3  

>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
           protein.
          Length = 1370

 Score = 52.8 bits (121), Expect = 3e-09
 Identities = 32/80 (40%), Positives = 48/80 (60%), Gaps = 2/80 (2%)
 Frame = +3

Query: 390 LNLSGNAISTLSRELY--LPNLQKLDLSRNQITLIESDAFYNMTALQKLDLSQNHISNVY 563
           L LSGNAI+++    +    +L++LDLS N++T +  DA  ++  L+ LDL +N ISN Y
Sbjct: 412 LTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVP-DALRDLALLKTLDLGENRISNFY 470

Query: 564 KEMFKSLINLERLILAQNQI 623
              F++L  L  L L  N I
Sbjct: 471 NGSFRNLDQLTGLRLIGNDI 490



 Score = 51.2 bits (117), Expect = 8e-09
 Identities = 32/84 (38%), Positives = 47/84 (55%), Gaps = 2/84 (2%)
 Frame = +3

Query: 381 VVSLNLSGNAISTLSRELY--LPNLQKLDLSRNQITLIESDAFYNMTALQKLDLSQNHIS 554
           ++ LNLS N ++ +   ++  L  LQ LDL  N I  IES+AF  +  L  L+LS N + 
Sbjct: 337 LIVLNLSYNMLTHIDARMFKDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLR 396

Query: 555 NVYKEMFKSLINLERLILAQNQIS 626
            V  ++F  L  L RL L+ N I+
Sbjct: 397 TVGAQLFNGLFVLNRLTLSGNAIA 420



 Score = 48.4 bits (110), Expect = 6e-08
 Identities = 31/83 (37%), Positives = 48/83 (57%), Gaps = 2/83 (2%)
 Frame = +3

Query: 381 VVSLNLSGNAISTLSRELY--LPNLQKLDLSRNQITLIESDAFYNMTALQKLDLSQNHIS 554
           +  L L GN I  LSR +   LPNLQ L+L+RN++  +E  AF     L+ + L  N +S
Sbjct: 480 LTGLRLIGNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAFERNMRLEAIRLDGNFLS 539

Query: 555 NVYKEMFKSLINLERLILAQNQI 623
           ++   +F S+ +L  L L++N I
Sbjct: 540 DI-NGVFTSIASLLLLNLSENHI 561



 Score = 31.1 bits (67), Expect = 0.009
 Identities = 23/83 (27%), Positives = 41/83 (49%), Gaps = 2/83 (2%)
 Frame = +3

Query: 390 LNLSGNAISTLSR--ELYLPNLQKLDLSRNQITLIESDAFYNMTALQKLDLSQNHISNVY 563
           L++ GN I +L    ++    ++ LD S N+IT  E        +++ L ++ N+I+ V 
Sbjct: 576 LDIHGNFIESLGNYYKIRDSKVKTLDASHNRIT--ELSPLSVPDSVELLFINNNYINLVR 633

Query: 564 KEMFKSLINLERLILAQNQISVM 632
              F   +NL R+ +  N I  M
Sbjct: 634 PNTFTDKVNLTRVDMYANMIETM 656



 Score = 30.7 bits (66), Expect = 0.012
 Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
 Frame = +3

Query: 444 NLQKLDLSRNQIT-LIESDAFYNMTALQKLDLSQNHISNVYKEMFKSLINLERLILAQNQ 620
           +++ LDLSRN+IT L E+    ++  LQ+L L +N I  +  +    L  L     + N 
Sbjct: 213 DIRILDLSRNEITRLQENSPLLDLRQLQELHLQRNAIVEIAGDALTGLTVLRTFNASYNS 272

Query: 621 I 623
           +
Sbjct: 273 L 273



 Score = 25.4 bits (53), Expect = 0.47
 Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
 Frame = +3

Query: 357 DLSKLWTIVVSL---NLSGNAISTLSRELYLPNLQKLDLSRNQITLIESDAFYNMTALQK 527
           D++ ++T + SL   NLS N I          NL+ LD+  N I  + +      + ++ 
Sbjct: 540 DINGVFTSIASLLLLNLSENHIEWFDYAFIPGNLKWLDIHGNFIESLGNYYKIRDSKVKT 599

Query: 528 LDLSQNHISNV 560
           LD S N I+ +
Sbjct: 600 LDASHNRITEL 610



 Score = 24.2 bits (50), Expect = 1.1
 Identities = 11/43 (25%), Positives = 24/43 (55%)
 Frame = +3

Query: 495 DAFYNMTALQKLDLSQNHISNVYKEMFKSLINLERLILAQNQI 623
           D+F  +  L  L++ ++++  +      SL NL+ L L +N++
Sbjct: 141 DSFLGLRELHTLEIVESNVQALPVNSLCSLDNLQTLNLTENRL 183


>AY569709-1|AAS86662.1|  408|Apis mellifera complementary sex
           determiner protein.
          Length = 408

 Score = 22.6 bits (46), Expect = 3.3
 Identities = 18/61 (29%), Positives = 29/61 (47%)
 Frame = +2

Query: 422 VERAVLAKSTETRFKQKPDNINRIGCILQYDSFAKVRPIAEPHQ*CVQGNV*KLNQFGEI 601
           + R +  KST T  + K D IN    IL   +    +PI E  +  ++  + K+N+  E 
Sbjct: 135 IHRDLPGKSTTTTVEVKRDTINPEDVILIRRTGEGSKPIFEREE--IKNVLTKINKIEEH 192

Query: 602 D 604
           D
Sbjct: 193 D 193


>AY569704-1|AAS86657.1|  426|Apis mellifera complementary sex
           determiner protein.
          Length = 426

 Score = 22.2 bits (45), Expect = 4.3
 Identities = 11/50 (22%), Positives = 27/50 (54%)
 Frame = +3

Query: 453 KLDLSRNQITLIESDAFYNMTALQKLDLSQNHISNVYKEMFKSLINLERL 602
           K+  S +  T+  ++ + N       +   N+  N YK+++ ++IN+E++
Sbjct: 313 KIISSLSNKTIHNNNNYNNNNYNNNYNNYNNNNYNNYKKLYYNIINIEQI 362


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 164,616
Number of Sequences: 438
Number of extensions: 3149
Number of successful extensions: 10
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19071468
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)