Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc30m01
         (669 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo...   120   1e-28
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c...    29   0.80 
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    27   1.8  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    26   5.6  
SPAC13G6.08 |||Cdc20/Fizzy family WD repeat protein|Schizosaccha...    25   7.5  
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    25   7.5  
SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni...    25   9.9  
SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo...    25   9.9  

>SPCC777.13 |vps35||retromer complex subunit
           Vps35|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 785

 Score =  120 bits (290), Expect = 1e-28
 Identities = 64/126 (50%), Positives = 79/126 (62%), Gaps = 3/126 (2%)
 Frame = +3

Query: 300 LRTSLLSPKSYYELYMAITDXXXXXXXXXXXXFQKG-RKVADLYELVQYAGNIVPRLYLL 476
           +R S L+PK YYELYM   +               G   + DLYELVQYAG+IVPRLYL+
Sbjct: 1   MRNSALTPKQYYELYMFNMESLRLLGGTLLETHLNGTHNLMDLYELVQYAGSIVPRLYLM 60

Query: 477 ITVGLVYIKTNTNLRRDLLKDLVEMCRGVQHPLRGLFLRNYLLQCXRNVLP--DTHEAPN 650
           ITVG  Y++T   L R+++ DL++MCRGVQHPLRGLFLR+YLL   R  LP     E   
Sbjct: 61  ITVGSAYLETPNALVREIMNDLLDMCRGVQHPLRGLFLRHYLLTQTRKGLPLGSEDEEDA 120

Query: 651 ENEGTV 668
             +GTV
Sbjct: 121 SRKGTV 126


>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1315

 Score = 28.7 bits (61), Expect = 0.80
 Identities = 15/59 (25%), Positives = 33/59 (55%)
 Frame = +3

Query: 63  VNAYEFSSNLQKNLIWFFNCIGLVTQAQMTNQVSPVEEQEKLLEEALNIVKVQAFQMKR 239
           V+ YE + N     + F++   +++QAQM N  SP+ +      + ++IV + A++ ++
Sbjct: 269 VSCYELTKNGVNRCV-FYSFSSMISQAQMLNATSPLLDPR--TTQIVSIVPIPAYESQQ 324


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 20/73 (27%), Positives = 31/73 (42%)
 Frame = -1

Query: 474  TGTI*VQCSQHIGPVHKDLQPSFPFGTPPEDTAPGDAVHLLLPYKAHNSSLVITVKFSIH 295
            T  +   C  H+  V K L     + +   + APG   HL+L    HNS  V+       
Sbjct: 1164 TSEVPTVCKDHVMDVLKQLFRKI-YASKDTEIAPGILGHLVLELSNHNS--VVRSSTQKL 1220

Query: 294  PALLMHVSGRPLI 256
             +LL  +S  P++
Sbjct: 1221 LSLLSELSNTPIV 1233


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 15/49 (30%), Positives = 26/49 (53%)
 Frame = +3

Query: 168 VEEQEKLLEEALNIVKVQAFQMKRCLDKSKLMDALKHASTMLGELRTSL 314
           VE +EKL  +  ++ + Q        ++ KL+D++K A T L + R  L
Sbjct: 371 VERREKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTELEQARIEL 419


>SPAC13G6.08 |||Cdc20/Fizzy family WD repeat
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 535

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 10/33 (30%), Positives = 23/33 (69%), Gaps = 1/33 (3%)
 Frame = -3

Query: 328 LFGDNSEVLNSPSIVDACFRASINL-DLSRHLF 233
           ++G N+E  N+P+++D   + + N+  +S++LF
Sbjct: 33  VYGQNAERSNTPTLIDPSNKETANVCPISKNLF 65


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 8/20 (40%), Positives = 14/20 (70%)
 Frame = +2

Query: 101 PYLVFQLHRISYSGPNDKPG 160
           PY ++QL  +  +GP++ PG
Sbjct: 388 PYNIYQLQELVRNGPDEHPG 407


>SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit
           Cwg2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 355

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
 Frame = +3

Query: 489 LVYIKTN-TNLRRDLLKDLVEMCRGVQHPLRGL 584
           L+++  N + + RDL+K+ VE+C+  Q   R +
Sbjct: 107 LLFLGDNLSRIDRDLIKNFVELCKTSQGHFRSI 139


>SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 569

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 12/30 (40%), Positives = 15/30 (50%)
 Frame = +1

Query: 22  NVNSVYENLLID*LLTPTNFRRICKKTLSG 111
           N+N +Y  +L D L  P N     K  LSG
Sbjct: 432 NINEMYRKILQDPLRFPDNIDEKAKDLLSG 461


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,870,430
Number of Sequences: 5004
Number of extensions: 60541
Number of successful extensions: 149
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)