BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc30l02
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 29 0.45
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 28 1.0
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 1.4
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 5.6
SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyc... 25 9.7
SPAC21E11.04 |ppr1||L-azetidine-2-carboxylic acid acetyltransfer... 25 9.7
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 25 9.7
SPBC19C2.10 |||BAR adaptor protein|Schizosaccharomyces pombe|chr... 25 9.7
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 29.1 bits (62), Expect = 0.45
Identities = 20/59 (33%), Positives = 25/59 (42%)
Frame = +2
Query: 371 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIF 547
EG M+L A PL + KD A + +K G R DLL AN+ IF
Sbjct: 59 EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIF 116
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 27.9 bits (59), Expect = 1.0
Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = -3
Query: 504 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 331
P F + P RK +A T+L SS P + RA SANS+T+P P A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573
Query: 330 KT 325
T
Sbjct: 574 TT 575
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.5 bits (58), Expect = 1.4
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = -3
Query: 465 ATSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPI 358
+T ++SG T SG + S+TTP+STP+
Sbjct: 574 STITSSASGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.4 bits (53), Expect = 5.6
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -3
Query: 411 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 289
+ R+ S N TPSS+P + SR + PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187
>SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1050
Score = 24.6 bits (51), Expect = 9.7
Identities = 10/31 (32%), Positives = 14/31 (45%)
Frame = +1
Query: 241 CCNWCCRTNCILTSLSNCVWSSFWTSTTCLP 333
CC CC + I SN ++ ST +P
Sbjct: 216 CCILCCLGSAIFAKASNALFVVIILSTISIP 246
>SPAC21E11.04 |ppr1||L-azetidine-2-carboxylic acid acetyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 209
Score = 24.6 bits (51), Expect = 9.7
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +3
Query: 36 TYVVHTYILM---FYLCIKQSRNSTEFYFFKAC 125
TY ++ I M + + IK +RN E +FFK C
Sbjct: 39 TYFINYEIEMGQTYPIDIKMTRNEAEDFFFKFC 71
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 235 KSCCNWCCRTNCILTSLSNCVW 300
KS +W C + ++S+SN VW
Sbjct: 447 KSSKSWRCMSQTHVSSISNLVW 468
>SPBC19C2.10 |||BAR adaptor protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 501
Score = 24.6 bits (51), Expect = 9.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -3
Query: 417 AKSGRAQSANSMTTPSSTPI 358
A++ QSA +TTPSS PI
Sbjct: 322 AQNSLGQSAVDLTTPSSPPI 341
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,090,822
Number of Sequences: 5004
Number of extensions: 38613
Number of successful extensions: 114
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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