BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc30e18
(637 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 29 0.56
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 26 4.0
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 5.2
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 5.2
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 26 5.2
SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces pom... 25 9.1
SPAC4H3.01 |||DNAJ domain protein Caj1/Djp1 type|Schizosaccharom... 25 9.1
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 29.1 bits (62), Expect = 0.56
Identities = 14/60 (23%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = +2
Query: 428 ECNV--KAVDSACMRCRRSFAVYPAVTYLHCGHSCLCTDCDETVNVDNTCPKCKSGIRYK 601
+CN+ + ++S +R F ++ +L C S T+ + + ++ C KCK +R++
Sbjct: 179 KCNLCYECIESDELRANCPFTDCNSINHLTCLASSFLTEECQVLPIEGMCTKCKRVLRWR 238
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 26.2 bits (55), Expect = 4.0
Identities = 10/43 (23%), Positives = 17/43 (39%)
Frame = -2
Query: 633 GQHVTKFCISNLYLMPLLHLGHVLSTFTVSSQSVHKHECPQCR 505
G C+ ++ GH+ ++ + K ECP CR
Sbjct: 252 GNRKCSLCMEFIHCPAATECGHIFCWSCINGWTSKKSECPLCR 294
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.8 bits (54), Expect = 5.2
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = -1
Query: 604 QFISNAAFTFRTRIVHVYRFVAIGAQTRMSAMQI 503
Q++SN + RT ++ + V+ +T+MSA Q+
Sbjct: 996 QYLSNMSHEIRTPLIGITGMVSFLLETQMSAEQL 1029
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 25.8 bits (54), Expect = 5.2
Identities = 21/69 (30%), Positives = 29/69 (42%), Gaps = 5/69 (7%)
Frame = +2
Query: 422 NTECNVKAVDSACMRCRRSFAVYPAVTYLH---CGHS-CLCTDCDETVNVDN-TCPKCKS 586
+ E V CM C + A+T + CGH+ C V+N +CP CK
Sbjct: 180 SAETTVPQECGLCMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICKH 239
Query: 587 GIRYKLEIQ 613
IR+ L Q
Sbjct: 240 RIRFALPDQ 248
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 25.8 bits (54), Expect = 5.2
Identities = 19/68 (27%), Positives = 29/68 (42%)
Frame = +2
Query: 386 LEIIKTDRNLPLNTECNVKAVDSACMRCRRSFAVYPAVTYLHCGHSCLCTDCDETVNVDN 565
+++IK D ++ + C C V P V+Y C H + DC +
Sbjct: 769 IQVIKNDIEKKRQLLGTIQDSEKVCDNCEGLLDV-PFVSY-SCLH-LVHRDCA----TET 821
Query: 566 TCPKCKSG 589
CPKCK+G
Sbjct: 822 VCPKCKAG 829
>SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 403
Score = 25.0 bits (52), Expect = 9.1
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = +2
Query: 131 KTVLCCAYVVRR 166
KT LCC+YV+R+
Sbjct: 63 KTKLCCSYVIRK 74
>SPAC4H3.01 |||DNAJ domain protein Caj1/Djp1
type|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 25.0 bits (52), Expect = 9.1
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = +3
Query: 309 GTAARCSRCGATTDV*LTLRAFATKRWKSLRPTETCRSTQNAM*RLSTVHACV 467
G R S CGA D+ LR + K PTE +A+ + T+ + V
Sbjct: 306 GKILRASWCGARYDIQGVLREACSNLLKKRVPTELRLKRAHALLEIGTIFSNV 358
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,588,417
Number of Sequences: 5004
Number of extensions: 51406
Number of successful extensions: 146
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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