Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc30b09
(641 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 157 1e-39
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 65 9e-12
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 62 5e-11
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 48 9e-07
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 40 2e-04
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 40 3e-04
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 36 0.004
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy... 26 5.3
SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch... 25 7.0
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 7.0
SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase Ogm2|Schizo... 25 9.3
>SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 194
Score = 157 bits (381), Expect = 1e-39
Identities = 76/164 (46%), Positives = 105/164 (64%), Gaps = 1/164 (0%)
Frame = +1
Query: 145 LQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYI 324
++ LL +Q DFV+ A+ S I ++K D++K ++ +M G++GDT FAEYI
Sbjct: 1 MESLLAVQGQDFVLTASSSSAVRGITVLKPDDDKSQILNSHNLMLYCGEAGDTTNFAEYI 60
Query: 325 AKNIQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENG-PELYFMD 501
A NI LY +R+ L P A ASFTR+ LA LRS PY VN+L+ GY+ G PEL+++D
Sbjct: 61 AANISLYTLRHNLNLSPEATASFTRKQLATSLRSRKPYQVNILLAGYETNLGKPELFWLD 120
Query: 502 YLASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKC 633
YLA+ V+VP+A GY + LSI DRY+K D T EA I+K C
Sbjct: 121 YLATCVRVPYACQGYSSFYCLSIFDRYYKPDLTIDEAVRIMKLC 164
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 64.9 bits (151), Expect = 9e-12
Identities = 39/162 (24%), Positives = 81/162 (50%)
Frame = +1
Query: 154 LLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKN 333
++G+ D +++ AD + +I + +K++ IS + G + DT I+ N
Sbjct: 38 IVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMISSN 97
Query: 334 IQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLAS 513
I+L+ + + A + +++L Y Y V +GGYD + GP L+ + S
Sbjct: 98 IELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYLV---LGGYDCK-GPHLFTIAAHGS 153
Query: 514 SVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQ 639
S K+P+ A G G ++S+++ ++ D EA +++K+ ++
Sbjct: 154 SDKLPYVALGSGSLAAISVLETKYQPDLERHEAMELVKEAIE 195
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 62.5 bits (145), Expect = 5e-11
Identities = 42/153 (27%), Positives = 71/153 (46%), Gaps = 2/153 (1%)
Frame = +1
Query: 154 LLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAK- 330
+L ++ D VMIAAD S+ + D E++ K+ D ++G GD D Q + K
Sbjct: 46 VLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLLEKL 105
Query: 331 NIQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKEN-GPELYFMDYL 507
I+ +GY L PS + + L PY+ +++ G D EN P + F D
Sbjct: 106 EIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAFADLR 165
Query: 508 ASSVKVPFAAHGYGGYLSLSIMDRYHKKDATET 606
++ P A G+ +L+L ++ + D +T
Sbjct: 166 GTTYSAPAIATGFAMHLALPMLRKATDDDRWKT 198
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 48.4 bits (110), Expect = 9e-07
Identities = 30/119 (25%), Positives = 54/119 (45%)
Frame = +1
Query: 157 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 336
+ I + F ++A D + + I + +++++ D LV+G G D + I + I
Sbjct: 17 VAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQRI 76
Query: 337 QLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLAS 513
LY + ++ + A R L Y + PY+V + G DKE E+Y D + S
Sbjct: 77 DLYHDNHERKMSAQSCACMVRTLL--YGKRFFPYYVYTTVAGIDKEGKGEIYSFDPVGS 133
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 40.3 bits (90), Expect = 2e-04
Identities = 27/115 (23%), Positives = 51/115 (44%), Gaps = 3/115 (2%)
Frame = +1
Query: 157 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 336
LGI D +++AA++ + ++ ++ EK+Y I D ++ V G + D N Y +
Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVG 94
Query: 337 QLYKMRNGYELGPSAAASFT---RRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 492
Q Y E+ ++ +Y P+ V+ L G+D G +L+
Sbjct: 95 QQYLQTFNEEMPCEQLVRRVCDLKQGYTQY-GGLRPFGVSFLYAGWDHIRGYQLF 148
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 39.9 bits (89), Expect = 3e-04
Identities = 31/152 (20%), Positives = 69/152 (45%)
Frame = +1
Query: 181 VMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNG 360
+++ D S +I +K+ +I+ L+ + G + D + + +L+++RN
Sbjct: 73 IVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRN- 131
Query: 361 YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAH 540
EL +AAS N+ Y + ++ G K G LY++D + +K +
Sbjct: 132 KELISVSAASKILSNIT-YSYKGYGLSMGTMLAGTGK-GGTALYYIDSDGTRLKGDLFSV 189
Query: 541 GYGGYLSLSIMDRYHKKDATETEAYDILKKCV 636
G G + ++D ++ D ++ EA + ++ +
Sbjct: 190 GSGSTFAYGVLDSGYRWDLSKQEALYLAQRSI 221
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 36.3 bits (80), Expect = 0.004
Identities = 38/169 (22%), Positives = 70/169 (41%), Gaps = 8/169 (4%)
Frame = +1
Query: 157 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 336
+G++ D V++ ++ + +M EK+++I + + G + D E+
Sbjct: 38 IGVKTKDAVVLGVEKRLTSPLM-ESHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQT 96
Query: 337 Q----LYKMRNGYELGPSA----AASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 492
Q Y G E + A F E S P+ V +L+ G D E+GP+LY
Sbjct: 97 QNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIAGID-EHGPQLY 155
Query: 493 FMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQ 639
+ + + A G G + S + + KD T EA ++ K ++
Sbjct: 156 HSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLILKVLR 204
>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 764
Score = 25.8 bits (54), Expect = 5.3
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +2
Query: 278 VSLVTQETQINLLNILQKIFSCIKCVMGMNWGPQLQPVS 394
VS+ ++ + LLN+L + SCI + P++ PVS
Sbjct: 673 VSIENSKSDMKLLNVLSRYTSCIGPGLFDIHSPRVPPVS 711
>SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 658
Score = 25.4 bits (53), Expect = 7.0
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -3
Query: 321 IFSKLICVS*VTNDTHY*SIRYLIDLFF 238
++SKLIC S V NDT IR + +F
Sbjct: 612 VYSKLICSSAVRNDTTRSLIRLIPKSYF 639
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 7.0
Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 1/116 (0%)
Frame = +1
Query: 295 GDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFTRRNLAEYLRSS-TPYFVNVLMGGYDK 471
GD ++ IAK++ K N +G A+ ++A+ LR PY VL +D+
Sbjct: 352 GDYSELLRVIAKSL---KDANVVVVG---VAALLLTHIAKALRKGFLPYTGIVLPSLFDR 405
Query: 472 ENGPELYFMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQ 639
+ + L + F + G + ++ HK +TE L +C+Q
Sbjct: 406 FKERKSSLVHSLLDAANAIFESCGLNDIMDETLEFLKHKNPQVKTETLRWLNRCLQ 461
>SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase
Ogm2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 739
Score = 25.0 bits (52), Expect = 9.3
Identities = 18/73 (24%), Positives = 29/73 (39%), Gaps = 2/73 (2%)
Frame = -3
Query: 375 GPQFIPITHFIQLNIFCNIFSKLICVS*VTNDTHY*SI-RY-LIDLFFIVFHDHNAVAVA 202
G IP+ +F LN + + +C V D H +I R+ L+D + F ++
Sbjct: 152 GTLCIPLVYFTALNFNYSFLAATLCTLMVALDNHLATISRFILLDSMLLFFIISTFFCLS 211
Query: 201 LIGCYHNKVVALY 163
YH Y
Sbjct: 212 RYHVYHKAPFTFY 224
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,404,299
Number of Sequences: 5004
Number of extensions: 48885
Number of successful extensions: 134
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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