Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc2p01
(736 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 246 3e-66
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 113 2e-26
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 74 2e-14
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 62 1e-10
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 53 4e-08
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 46 7e-06
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 39 8e-04
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 34 0.024
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 33 0.042
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 32 0.098
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 28 1.6
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 27 2.8
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 246 bits (602), Expect = 3e-66
Identities = 112/193 (58%), Positives = 143/193 (74%)
Frame = +1
Query: 157 GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQK 336
GF FE +QRN L +KGFP PKAT TGTTIVG+I D ++LGADTRAT +++DKNC+K
Sbjct: 9 GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68
Query: 337 IHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH 516
+H ++ N++C GAGTAADTE T ++S +EL L+T R V TA T+LK+ LFRYQGH
Sbjct: 69 LHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGH 128
Query: 517 IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKK 696
IGA LVLGG D GPH++ I HGS DKLPY +GSGSLAA++V E ++ D+ E +
Sbjct: 129 IGAYLVLGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDLERHEAME 188
Query: 697 LVRDAIAAGIFND 735
LV++AI AGIFND
Sbjct: 189 LVKEAIEAGIFND 201
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 113 bits (273), Expect = 2e-26
Identities = 58/170 (34%), Positives = 94/170 (55%), Gaps = 1/170 (0%)
Frame = +1
Query: 229 KTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQ 408
+ GTTI + Y DGVIL AD+R T ++++ K+ L N++CC +G+AADT+
Sbjct: 22 RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81
Query: 409 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCIYPH 585
+ L + R+ G V TAATL M ++ + + A L++ G D +TG +Y I
Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLG 141
Query: 586 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFND 735
GS+ K P A GSGS +A ++ +M +EE + +++A+A + D
Sbjct: 142 GSLHKQPLAIGGSGSAFIYGFCDANFRENMTQEEAVEFLKNAVALAMERD 191
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 74.1 bits (174), Expect = 2e-14
Identities = 51/200 (25%), Positives = 94/200 (47%), Gaps = 3/200 (1%)
Frame = +1
Query: 145 IPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDK 324
+PVP S + RN K GTT + Y G+++ D+RA+ +++ +
Sbjct: 33 VPVPQSSL--YLRNLTDETKNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQ 90
Query: 325 NCQKIHYLASNMYCCG--AGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRML 498
+K+ + N Y G AG AAD + + + L +L + V A+ +L +
Sbjct: 91 TVKKV--IEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSAASKILSNIT 148
Query: 499 FRYQGH-IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 675
+ Y+G+ + +L G + G +Y I G+ K ++GSGS A V ++G++ D+
Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALYYIDSDGTRLKGDLFSVGSGSTFAYGVLDSGYRWDL 208
Query: 676 NEEEGKKLVRDAIAAGIFND 735
+++E L + +I A D
Sbjct: 209 SKQEALYLAQRSIVAATHRD 228
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 61.7 bits (143), Expect = 1e-10
Identities = 43/174 (24%), Positives = 79/174 (45%), Gaps = 11/174 (6%)
Frame = +1
Query: 214 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 393
A +A K G+T +G+ D V+LG + R T + ++ + +K+ + S++ C +G AD
Sbjct: 27 AIEAIKLGSTAIGVKTKDAVVLGVEKRLT-SPLMESHSVEKLFEIDSHIGCAISGLTADA 85
Query: 394 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY-QGH----------IGAALVLG 540
+ Q + R +E+ + + R+ +G G AL++
Sbjct: 86 RTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIA 145
Query: 541 GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLV 702
G+D GP +Y P G+ + +GSGS A + + +DM EE + L+
Sbjct: 146 GIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLI 199
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 53.2 bits (122), Expect = 4e-08
Identities = 37/158 (23%), Positives = 73/158 (46%), Gaps = 7/158 (4%)
Frame = +1
Query: 247 VGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQL 426
+GI+ DG++L A+ + T + +++ +K++++ NM C AG AD + + A ++
Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILI-NYARRV 93
Query: 427 ELQRLHT-GRTVPVETAATL---LKRMLFRYQG--HIGAALVLGGVDR-TGPHIYCIYPH 585
Q L T +P E LK+ +Y G G + + G D G ++ P
Sbjct: 94 GQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPS 153
Query: 586 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKL 699
G+ ++G S + ++ +K D+N +E +
Sbjct: 154 GNYGSWQANSIGGNSTSVQSLMRQEYKDDINLDEASAM 191
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 45.6 bits (103), Expect = 7e-06
Identities = 46/193 (23%), Positives = 78/193 (40%), Gaps = 6/193 (3%)
Frame = +1
Query: 160 FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKI 339
FS F N L Q + A A G T VGI DGV+L + + T + + +K+
Sbjct: 7 FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIG-ASLEKV 64
Query: 340 HYLASNMYCCGAGTAADTEMTTQSV--ASQLELQRLHTG----RTVPVETAATLLKRMLF 501
+ ++ +G D + +Q ++++ + + E A+ + +
Sbjct: 65 CAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQS 124
Query: 502 RYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNE 681
G +L++ G+D GP +Y + P G+ +G S AA E KR +E
Sbjct: 125 GGVRPFGVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLE---KRYNDE 181
Query: 682 EEGKKLVRDAIAA 720
E V AI A
Sbjct: 182 LELDDAVHTAILA 194
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 38.7 bits (86), Expect = 8e-04
Identities = 29/132 (21%), Positives = 61/132 (46%), Gaps = 4/132 (3%)
Frame = +1
Query: 217 PKATKTGTTIVGIIYADG-VILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 393
P GTT+ I DG IL DTR+ ++ + ++H + ++ +G AD
Sbjct: 8 PYVQNGGTTVA--IAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADA 65
Query: 394 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF--RYQGHIGAALVLGGVDRTGP-H 564
+ + +++L + R + ++ A +++ +L+ R+ + + G+D+ G
Sbjct: 66 LALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYY-VYTTVAGIDKEGKGE 124
Query: 565 IYCIYPHGSVDK 600
IY P GS ++
Sbjct: 125 IYSFDPVGSYER 136
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 33.9 bits (74), Expect = 0.024
Identities = 35/147 (23%), Positives = 54/147 (36%), Gaps = 5/147 (3%)
Frame = +1
Query: 214 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 393
A KA + +T +GI DGVIL + T + N +I + ++ G D
Sbjct: 26 AYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVN-NRIGSVDRHIGIATTGFIPDG 84
Query: 394 EMTTQSVASQLELQRLHTGRTVPVETAATLLKR--MLFRYQGHI---GAALVLGGVDRTG 558
+ + + R + G +P A L LF + G + D G
Sbjct: 85 QHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEG 144
Query: 559 PHIYCIYPHGSVDKLPYATMGSGSLAA 639
PH+Y + P+G A G G A
Sbjct: 145 PHLYMVEPNGVYWGYNGAAAGKGRQVA 171
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 33.1 bits (72), Expect = 0.042
Identities = 35/152 (23%), Positives = 62/152 (40%), Gaps = 5/152 (3%)
Frame = +1
Query: 214 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 393
A +A K G+ VG++ +L A R E K K+ + ++ AG A D
Sbjct: 25 ALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQK---KLIRIDDHIGIAIAGLAPDA 81
Query: 394 EMTTQSVASQLELQRLHTGRTVPVETAAT-LLKRMLFRYQGH----IGAALVLGGVDRTG 558
+ + + + + R +PV + + ++ Q + G ++ G D +G
Sbjct: 82 RVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGYDESG 141
Query: 559 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 654
PH+ P G V + +MGS S +A E
Sbjct: 142 PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIE 173
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 31.9 bits (69), Expect = 0.098
Identities = 16/73 (21%), Positives = 39/73 (53%)
Frame = +1
Query: 232 TGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQS 411
TG++++ + +ADGV++ AD A+ ++ + +++ + N G +D + Q
Sbjct: 42 TGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQ-IQR 100
Query: 412 VASQLELQRLHTG 450
+ +LE++ + G
Sbjct: 101 LLEKLEIKEGNYG 113
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -2
Query: 591 RSMRINAVNMRTGSVNTSQNQSSPYMTLIAKQH 493
+S+ + M+TGSVN +Q+Q M ++A Q+
Sbjct: 267 QSIPSGPMGMKTGSVNDTQSQQITLMNVLASQN 299
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 27.1 bits (57), Expect = 2.8
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = -2
Query: 576 NAVNMRTGSVNTSQNQSSPYMTLIAKQHSFQQCGSSF 466
N + M+ S+ T + + +PY L+A + CGS +
Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,025,415
Number of Sequences: 5004
Number of extensions: 63569
Number of successful extensions: 170
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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