Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc2d08
(760 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual 110 2e-25
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 67 3e-12
SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces pomb... 64 2e-11
SPBC725.07 |pex5||peroxisomal targeting signal receptor |Schizos... 30 0.41
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 29 0.95
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 26 5.1
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub... 26 6.7
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 6.7
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 26 6.7
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 8.9
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 25 8.9
>SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1029
Score = 110 bits (265), Expect = 2e-25
Identities = 66/223 (29%), Positives = 113/223 (50%), Gaps = 5/223 (2%)
Frame = +1
Query: 94 LIEILRATI--DPNQRQQAEEQLSQIHKIIGFAPALLQVVMQNDVNIPVRQAGVVYLKNL 267
L+E AT+ DPN R +AE L Q+ K F A+LQ++ ++++P +QA V+YLKN
Sbjct: 3 LVEHFDATLSADPNTRTKAELSLKQLEKEPSFVLAVLQLLSSQEISLPTQQAAVIYLKNR 62
Query: 268 ITSGWQDKEREEGEPIPFNIHEQDRAMIRDIIVEAIVQAPEIIRVQLCVCLKTIIKHDFP 447
++ W + P P +I E+ +A+ R I+ ++Q+P R L L I+ DFP
Sbjct: 63 VSRSWSSID---DAPSPLDIPEEQKALFRQNILPVLLQSPMSTRSHLMAILNIILSTDFP 119
Query: 448 ERWTQIVDKIHIFLQNPDANSWMGALQCLYQLIKNYEYHISEKRTPLIEAMNLLLPMIYN 627
E W + + + + L C ++L K Y + + +++ + + L P I
Sbjct: 120 EYWPGFSEYTSNLVHSTERCEVYAGLICFHELAKVYRWRLDDRQRDIGPLVAALFPTILQ 179
Query: 628 L---IVNLEPDQSVESILIQKQILKCFYALTKYILPLDLITRD 747
L ++NLE + S E + + ILK F ++ LP +L+ D
Sbjct: 180 LGQGLINLEDNDSAEML---RLILKTFKSVIALELPPELLAND 219
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 66.9 bits (156), Expect = 3e-12
Identities = 52/212 (24%), Positives = 104/212 (49%), Gaps = 2/212 (0%)
Frame = +1
Query: 91 KLIEILRATIDPNQRQQAEEQLSQIHKIIGFAPALLQVVMQNDVNIPVRQAGVVYLKNLI 270
KL+E +++ DPN R QAE L + K FA L + + ++P+RQ ++YL+ I
Sbjct: 8 KLLEQVQSA-DPNSRIQAELGLRDLEKYHDFAAKLTDIA-SSGASVPLRQGSLIYLQRYI 65
Query: 271 TSGWQDK-EREEGEPIPFNIHEQDRAMIRDIIVEAIVQAPEIIRVQLCVCLKTIIKH-DF 444
W E+ + PIP E + +R+ ++ +V ++ ++I + D+
Sbjct: 66 VHHWSPLFEQFQDGPIP---DENVKKHVRETLLHLLVSLDNFTLIKAVAYAVSLIANVDY 122
Query: 445 PERWTQIVDKIHIFLQNPDANSWMGALQCLYQLIKNYEYHISEKRTPLIEAMNLLLPMIY 624
P+ W ++V + LQ+ + NS +L L +L+ + + E++ +I L ++Y
Sbjct: 123 PDEWPEVVPAVLHLLQSTNENSINASLDVLDELV---DESLVEEQFFIIAPQ--LASILY 177
Query: 625 NLIVNLEPDQSVESILIQKQILKCFYALTKYI 720
I + P+ S+ ++Q + +K F + + I
Sbjct: 178 QFIFSAPPNDSMR--MLQARGIKLFRSCLELI 207
>SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 967
Score = 64.5 bits (150), Expect = 2e-11
Identities = 43/152 (28%), Positives = 76/152 (50%), Gaps = 1/152 (0%)
Frame = +1
Query: 103 ILRATIDPNQRQQAEEQLSQIH-KIIGFAPALLQVVMQNDVNIPVRQAGVVYLKNLITSG 279
+L T++P + AEE L + FA LL +V ++ V+I ++ A +Y KN I
Sbjct: 7 LLARTLNPTTSKSAEEALKVWELQDSSFALKLLNIVAEDTVDINIKLAASLYFKNYIKKH 66
Query: 280 WQDKEREEGEPIPFNIHEQDRAMIRDIIVEAIVQAPEIIRVQLCVCLKTIIKHDFPERWT 459
W + EEG I I ++ +I+ I+ ++++ II+VQL + I DFP+RW
Sbjct: 67 W---DSEEGASI--RISDEVAELIKREIINLMLKSTTIIQVQLGEVIGYIANFDFPDRWD 121
Query: 460 QIVDKIHIFLQNPDANSWMGALQCLYQLIKNY 555
++ + L D N+ + L + + K +
Sbjct: 122 TLLPDLISKLSAVDMNTNIAVLSTAHAIFKRW 153
>SPBC725.07 |pex5||peroxisomal targeting signal receptor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 29.9 bits (64), Expect = 0.41
Identities = 22/91 (24%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
Frame = +1
Query: 355 DIIVE-AIVQAPEIIRVQLCVCLKTIIKHDFPERWTQIVDKIHIFLQNPDANSWMGALQC 531
D++++ A+ + + VQ VCL+ I + FP+ + KI+ + D+ + + +Q
Sbjct: 363 DLMMDLAVSYVNQSLNVQALVCLEDWIVNSFPQYRNRFA-KINERFEEKDSANDLLKMQ- 420
Query: 532 LYQLIKNYEYHISEKRTPLIEA-MNLLLPMI 621
+Y L YE +++KR+ ++A + +++ M+
Sbjct: 421 MYFLDVAYELSLAKKRSSKVQAGLGIIMYML 451
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 28.7 bits (61), Expect = 0.95
Identities = 24/102 (23%), Positives = 51/102 (50%), Gaps = 8/102 (7%)
Frame = +1
Query: 415 CLKTIIKHDFPERWTQIVD----KIHIFLQNPDAN--SWMGALQCLYQLIKN-YEYHISE 573
C++ +H W ++ + K +F N + + S++G+ Y++ K+ + I+
Sbjct: 831 CIEVRTRHRLHIPWLKMPNFDFKKNQVFFGNYEDSKLSFIGSNHTRYRIDKDEFSSGIAS 890
Query: 574 KRTPLIEAMNLLLPMIY-NLIVNLEPDQSVESILIQKQILKC 696
R L+ ++ P+I ++I+ L+P + S LI + LKC
Sbjct: 891 IRGTLMSSVLPDAPLIIRDMIIRLKPPKFCNSALIDPEFLKC 932
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 26.2 bits (55), Expect = 5.1
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +1
Query: 460 QIVDKIHIFLQNPDANSWMGALQCLYQLIKNYEYHISEKRTPLIEAM 600
Q +K + QNPD LQ + +IKN I++ L++ +
Sbjct: 1005 QFFEKAYKLFQNPDLEVSQETLQVIISVIKNRRSCIADVYNELLQGL 1051
>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 838
Score = 25.8 bits (54), Expect = 6.7
Identities = 11/23 (47%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -2
Query: 600 HSF-YEWRSFLGNVVFIVLDQLI 535
H F Y W +FL NVV+ V+ Q++
Sbjct: 532 HFFDYPWNNFLHNVVYDVVQQVL 554
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.8 bits (54), Expect = 6.7
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = -2
Query: 459 SPSLWKVMFYDSLQADTQLYSDYFRSLDDRLYYDVSN 349
S S+ KV+ + + ++ L S YF ++ DRLY D+ N
Sbjct: 736 SYSINKVVHHLNYFMNSFLSSTYFDAVKDRLYADLPN 772
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 106 LRATIDPNQRQQAEEQLSQIHKIIGFAPAL 195
LR + + ++E LSQ+HKI+G A L
Sbjct: 2732 LRRLFELVKNHYSQELLSQVHKIVGSADFL 2761
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.4 bits (53), Expect = 8.9
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = +1
Query: 505 NSW-MGALQCLYQLIKNYEYHISEKRTPLIEAMNLLLPMIYNLIVNLEPDQSVESILIQK 681
N W MG L+ L +L Y ++ R L + LLL + + N+EP + + L+QK
Sbjct: 1026 NPWLMGILKLLVEL-----YQFADFRLNLKFEIELLLNNLNVKMDNIEPSEMYRNHLVQK 1080
Query: 682 QILK 693
L+
Sbjct: 1081 ADLE 1084
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 25.4 bits (53), Expect = 8.9
Identities = 12/48 (25%), Positives = 25/48 (52%)
Frame = -2
Query: 681 LLYQN*FNRLIWFQVNYEIIYHREKQVHSFYEWRSFLGNVVFIVLDQL 538
L +Q +IW Q + +YH+E + + +R+ + V+ ++ D L
Sbjct: 104 LFFQASGQGIIWGQAGWFSVYHQELVISAITRYRNEIKRVLGVLEDIL 151
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,314,777
Number of Sequences: 5004
Number of extensions: 70862
Number of successful extensions: 206
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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