BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc29h23
(616 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 29 0.71
SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|... 29 0.71
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 27 1.6
SPBC13E7.11 ||SPBC30D10.19c|mitochondrial rhomboid protease|Schi... 27 2.2
SPBC19C7.08c |||leucine carboxyl methyltransferase|Schizosacchar... 26 3.8
SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 26 5.0
SPACUNK4.17 |||NAD binding dehydrogenase family protein|Schizosa... 25 6.6
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 25 6.6
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 8.7
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 25 8.7
SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr 1||... 25 8.7
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 25 8.7
>SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 270
Score = 28.7 bits (61), Expect = 0.71
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +1
Query: 220 FYSRNRWVEYSDKYYLNYDGSQVPAEW 300
F + WV Y+D LNYDGS W
Sbjct: 139 FEKKAAWVLYADIICLNYDGSAFDYAW 165
>SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 381
Score = 28.7 bits (61), Expect = 0.71
Identities = 18/73 (24%), Positives = 31/73 (42%), Gaps = 1/73 (1%)
Frame = +1
Query: 121 ASLYKLYRQDDVKDGVLVGEDKYGNKYYENPRFFYSRNRWVEYSDKYYLNYDGSQVP-AE 297
+S+ L + VKD + D G+ +N + S N Y +K+ YD +P +
Sbjct: 47 SSMLHLSKGLTVKDVRIFFHDSIGSTLLKNRKKLNSENELPNYGEKFTHKYDNQDMPDSV 106
Query: 298 WFGWLHYKTDLPP 336
W ++ T P
Sbjct: 107 WLAKVNGMTKSDP 119
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 27.5 bits (58), Expect = 1.6
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
Frame = +1
Query: 4 LYGSCTPLVISTKMSLGKFLALDKLSN--FFNVIRQNGGIRASLYKLYR 144
L G + LV+ L ++L +K N FF ++ + I SLY LYR
Sbjct: 165 LEGMASLLVVQALGHLSRWLVHNKSENWMFFILLNASSAISMSLYLLYR 213
>SPBC13E7.11 ||SPBC30D10.19c|mitochondrial rhomboid
protease|Schizosaccharomyces pombe|chr 2|||Manual
Length = 298
Score = 27.1 bits (57), Expect = 2.2
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +2
Query: 371 WRTTPKIFLARPLNMCRTVRPDPKWKP 451
W+ K F RP ++P P WKP
Sbjct: 11 WKFGQKRFYYRPWIRIENIKPTPLWKP 37
>SPBC19C7.08c |||leucine carboxyl
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 681
Score = 26.2 bits (55), Expect = 3.8
Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +3
Query: 342 GSESSPLQVDGGP-HRKSFWHDRSICAVQYDPTQSGSLGAETQRQ 473
G +S P+ V G R W + S+C D + +G ET+++
Sbjct: 594 GDDSKPIIVGGAAVFRTILWEEESVCINMNDYSVTGVCIEETEKR 638
>SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 693
Score = 25.8 bits (54), Expect = 5.0
Identities = 10/39 (25%), Positives = 17/39 (43%)
Frame = +1
Query: 181 DKYGNKYYENPRFFYSRNRWVEYSDKYYLNYDGSQVPAE 297
DKY Y YS N W+++ K+ + + P +
Sbjct: 565 DKYNKDIYIGSTVCYSPNEWLDFFSKFQQPREALKFPED 603
>SPACUNK4.17 |||NAD binding dehydrogenase family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 405
Score = 25.4 bits (53), Expect = 6.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 178 EDKYGNKYYENPRFFYSRNRWVEYSDKY 261
E +Y + Y N R FYS + ++EY D +
Sbjct: 90 ETEYASSY-ANTREFYSADEYLEYLDSH 116
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 25.4 bits (53), Expect = 6.6
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 419 RTVRPDPKWKPGSRNAKTVFKNK 487
R+VR PK+KPG+R + T +N+
Sbjct: 110 RSVRSKPKYKPGTRRS-TRLRNR 131
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +1
Query: 154 VKDGVLVGEDKYGNKYYENPRFFYSRNRWVEYSDKYY 264
V G+++ + +K ENP F+Y R EY D +Y
Sbjct: 357 VSYGLMMNLVRDFSKNLENPNFYYER----EYVDSFY 389
>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 25.0 bits (52), Expect = 8.7
Identities = 12/56 (21%), Positives = 26/56 (46%)
Frame = +1
Query: 364 KWMADHTENLSGTTAQYVPYSTTRPKVEAWEPKRKDSL*K*IRAKNHLVEHLFFFV 531
KW + + ++ A Y+P+ +EA+ +S + K +L +F+F+
Sbjct: 355 KWASPDSSSILSWYAMYIPFMAANGVLEAFYVSAANSSQLYDQGKCYLASAVFYFI 410
>SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 469
Score = 25.0 bits (52), Expect = 8.7
Identities = 16/63 (25%), Positives = 26/63 (41%), Gaps = 1/63 (1%)
Frame = +1
Query: 250 SDKYYLNYDGSQVPAEWFGWLHYKTDLPPHQDPSRPHYKWMADHTENLSGTTAQ-YVPYS 426
S+ + +N +G +V + W + D H D + + H E L T + Y Y
Sbjct: 302 SNTFVVNNEGKRVRGRRYPWGVVEVDNEEHSDFPKLREMLIRTHLEELKEQTNKLYEAYR 361
Query: 427 TTR 435
T R
Sbjct: 362 TER 364
>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 683
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +1
Query: 235 RWVEYSDKYYLNYDGSQVPAEWFGWLHYKTD 327
+WV+YS+KY L Y Q+ E G +H+ D
Sbjct: 496 KWVDYSNKYGLGY---QLSDESVG-VHFNDD 522
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,803,180
Number of Sequences: 5004
Number of extensions: 65057
Number of successful extensions: 197
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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