Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc29h18
         (533 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc...   166   2e-42
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...    38   7e-04
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    33   0.020
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    33   0.020
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc...    26   3.1  
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    26   4.1  
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ...    25   5.4  
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc...    25   5.4  
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo...    25   7.1  
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos...    25   7.1  
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc...    25   7.1  
SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces ...    25   7.1  
SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz...    25   9.4  
SPAC7D4.11c |sec39||secretory pathway protein Sec39 |Schizosacch...    25   9.4  

>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 240

 Score =  166 bits (404), Expect = 2e-42
 Identities = 74/147 (50%), Positives = 113/147 (76%)
 Frame = +2

Query: 83  MVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 262
           MV++A ++  + GKA++SRQF EM++ R+E LL++FP L++   Q+T VE+++VR+VYQP
Sbjct: 1   MVVLAVSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKSQNTTVESDNVRFVYQP 60

Query: 263 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 442
           LD+LY++LIT   SNIL+D++TL L S+VV   C  L E E+L  AF +  AFDE  +LG
Sbjct: 61  LDELYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLG 120

Query: 443 YRESVNLAQVRSFVEMDSHEEKIYQAV 523
           YR++V+L Q+++++EM+SHEEKI + V
Sbjct: 121 YRDNVSLTQIKTYLEMESHEEKIQEIV 147


>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 38.3 bits (85), Expect = 7e-04
 Identities = 29/140 (20%), Positives = 63/140 (45%), Gaps = 2/140 (1%)
 Frame = +2

Query: 95  AATVCTKSGKALVSRQF-VEMTKARIEGLLAAFPKLMTG-GRQHTFVETESVRYVYQPLD 268
           A  V    GK ++SR +  ++  + +E  L    ++    G     +  E + Y+Y   +
Sbjct: 4   AIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYIHHN 63

Query: 269 KLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYR 448
            +Y+L ++   S+ +E L  LR  + V  +Y  +L E  + +    +    DEI+  G+ 
Sbjct: 64  DVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDFGFP 123

Query: 449 ESVNLAQVRSFVEMDSHEEK 508
           ++     ++ ++   S+  K
Sbjct: 124 QTTETKILQEYITQTSNTVK 143


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 33.5 bits (73), Expect = 0.020
 Identities = 20/76 (26%), Positives = 34/76 (44%)
 Frame = +2

Query: 215 QHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLN 394
           +H  V   S  Y+Y   + LY++ IT    N++  LE L    + +  Y  +L E  V +
Sbjct: 43  RHPIVSIGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKD 102

Query: 395 QAFNLLFAFDEIVALG 442
               +    DE++  G
Sbjct: 103 NVSFIFELLDEMIDYG 118


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 33.5 bits (73), Expect = 0.020
 Identities = 28/128 (21%), Positives = 51/128 (39%), Gaps = 3/128 (2%)
 Frame = +2

Query: 113 KSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQH---TFVETESVRYVYQPLDKLYML 283
           + GK  +S+ +V         L A   +L++   Q     F+E E+ + VY+    LY  
Sbjct: 10  RHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVYRRYAGLYFC 69

Query: 284 LITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYRESVNL 463
                  N L  LE +  F  ++  +   + E +++   + +    DEI+  G     N 
Sbjct: 70  FCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEIILGGEIGESNK 129

Query: 464 AQVRSFVE 487
             V   +E
Sbjct: 130 KSVLERIE 137


>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 18/87 (20%), Positives = 37/87 (42%)
 Frame = +2

Query: 224 FVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAF 403
           FVE +  + VY+    L+ +    +  N L  LE +  F   + +Y   + E +++    
Sbjct: 50  FVEYKGEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFE 109

Query: 404 NLLFAFDEIVALGYRESVNLAQVRSFV 484
              +  +E++  G  +  +   V S V
Sbjct: 110 KAYYVMEELLLAGELQESSKTNVLSAV 136


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = -3

Query: 372 SCTQYSGTTLLKSLNVSRSSRMLLAFVVISNIYN 271
           SC      + L S+N + +SR LLA +V  +I N
Sbjct: 134 SCDSNENLSTLSSVNQNFNSRQLLATIVPESIQN 167


>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 2344

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 8/28 (28%), Positives = 15/28 (53%)
 Frame = -3

Query: 222  VCCRPPVISLGKAASRPSMRAFVISTNW 139
            +CC P  +++ +   +PS R+F     W
Sbjct: 2128 LCCDPSRLNIEQELQKPSGRSFACKKRW 2155


>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
           Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1141

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 14/36 (38%), Positives = 26/36 (72%), Gaps = 1/36 (2%)
 Frame = +2

Query: 206 GGRQHTFVE-TESVRYVYQPLDKLYMLLITTKASNI 310
           GGR  T +E   ++++ Y+ L KL++ LIT+K+S++
Sbjct: 664 GGRIVTRIEFLRALQFTYE-LRKLFIELITSKSSSV 698


>SPCC18.03 |||shuttle craft like transcriptional
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1077

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 8/12 (66%), Positives = 9/12 (75%)
 Frame = -2

Query: 235 CFYKCMLPSSCH 200
           C Y C+LP SCH
Sbjct: 642 CPYLCVLPKSCH 653


>SPAC212.04c |||S. pombe specific DUF999 family protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 288

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 3/69 (4%)
 Frame = +2

Query: 8   SNLWKPILKYFGVF-YEN*I*EYKNKMVLIAATVCTK--SGKALVSRQFVEMTKARIEGL 178
           S +W PI+ Y G+F        Y +K+++ A  + +   +   +V   F  +  A I G+
Sbjct: 142 SEIWGPIVIYVGLFILLLSAFNYCSKLIIKALPLISMVIAWVGVVIAAFSVIITATIAGV 201

Query: 179 LAAFPKLMT 205
           +AAF  ++T
Sbjct: 202 IAAFSVIIT 210


>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 313

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 16/42 (38%), Positives = 27/42 (64%), Gaps = 3/42 (7%)
 Frame = +2

Query: 236 ESVRYVYQPLDKLYMLLITTKASNILED--LET-LRLFSRVV 352
           ++VR V+Q LD+L  +L  +K   +L D   ET +RL++ +V
Sbjct: 197 KNVRVVFQGLDRLEQVLKESKGPFLLGDHLTETDVRLYTTIV 238


>SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 282

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 13/41 (31%), Positives = 26/41 (63%)
 Frame = +2

Query: 137 RQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQ 259
           +Q +E+++  ++ LL+   + ++     TF +TESVR +YQ
Sbjct: 223 QQLLELSRTELKRLLSKVNEKISD----TFPDTESVRQLYQ 259


>SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein
           Utp5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 666

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 24/93 (25%), Positives = 37/93 (39%), Gaps = 6/93 (6%)
 Frame = +1

Query: 178 TGGLP*TNDRRTAAYICRNRVRQICL------PATRQVVYVTDHDKG**HPGRSGDIETL 339
           TG  P    +   A +C N V+ + +      P T  +  +T+   G      +  +  L
Sbjct: 242 TGEFPTNTVKNVGALVCENEVKMLSIAYEIENPETGVLAALTND--GTIEMFENPWLSKL 299

Query: 340 QQSGTRILCTAHRN*SLEPGLQFAICFRRDCRS 438
           +Q+GT     +HR   L       ICF   CRS
Sbjct: 300 RQNGTNASSLSHRRKLLTSHSTLKICF---CRS 329


>SPAC7D4.11c |sec39||secretory pathway protein Sec39
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 769

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 3/48 (6%)
 Frame = -3

Query: 492 SISTNERTCARLTLSLYPRATISSKANSKLKA---WFKTSVSVSCTQY 358
           S+  +  T + L+ SL    T +    ++LK     FKTSVS+SC+ +
Sbjct: 319 SLQNDLTTPSSLSTSLLMEITKAIALLTQLKVPAPTFKTSVSISCSNF 366


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,240,226
Number of Sequences: 5004
Number of extensions: 43849
Number of successful extensions: 109
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)