Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc28l12
         (626 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp...   134   7e-33
SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subun...    68   1e-12
SPBC776.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    29   0.73 
SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    26   5.1  
SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    26   5.1  
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce...    25   6.8  

>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
           subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1210

 Score =  134 bits (325), Expect = 7e-33
 Identities = 71/149 (47%), Positives = 94/149 (63%), Gaps = 12/149 (8%)
 Frame = +2

Query: 179 LWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSGEMET 358
           L QE CW VI+++F+E  L RQQL SFDEF+Q ++Q IV+D   + L   AQH   + + 
Sbjct: 12  LTQEDCWTVISSFFEETSLARQQLFSFDEFVQNTMQEIVDDDSTLTLDQYAQHTGAQGDV 71

Query: 359 PPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIE------- 517
             +Y + F QIYLS+PT  E DG+ + M P EARLRNLTYS+PLYVD+ K +        
Sbjct: 72  TRRYEINFGQIYLSRPTMTEADGSTTTMFPQEARLRNLTYSSPLYVDMRKKVMVAADSNV 131

Query: 518 --KEHEDPIETQHQ---KTFIGKIPIMLK 589
              E E  +E + +   K FIGKIPIML+
Sbjct: 132 PIGEEEWLVEEEDEEPSKVFIGKIPIMLR 160



 Score = 25.8 bits (54), Expect = 5.1
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +3

Query: 555 KHLLARFLLCLRSTYCLLNSLTD 623
           K  + +  + LRST+C+LN ++D
Sbjct: 149 KVFIGKIPIMLRSTFCILNGVSD 171


>SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subunit
           Rpc2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1165

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 40/131 (30%), Positives = 70/131 (53%)
 Frame = +2

Query: 197 WIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSGEMETPPKYRL 376
           W ++ A+   KGLV+Q LDS++ F+ + +++IV+ +  +    E           P + L
Sbjct: 51  WQLLPAFLKVKGLVKQHLDSYNYFVDVDLKKIVQANEKVTSDVE-----------PWFYL 99

Query: 377 KFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEHEDPIETQHQK 556
           K+  I +  P   + D   + + P+E RLR+LTY A +YVDI  T  K+       + + 
Sbjct: 100 KYLDIRVGAPVRTDADAIQASISPHECRLRDLTYGANIYVDIEYTRGKQ-----VVRRRN 154

Query: 557 TFIGKIPIMLK 589
             IG++P+ML+
Sbjct: 155 VPIGRMPVMLR 165


>SPBC776.16 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 253

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 19/66 (28%), Positives = 31/66 (46%)
 Frame = +2

Query: 320 QAEAQHFSGEMETPPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVD 499
           Q+E+  F        + ++KF+Q++ S P     + A S +  + ARL  L  S P    
Sbjct: 4   QSESHAFINNAPKEDRIQVKFEQLFESLPLPLRAEEALSKLRHDSARLMILKTSDPTLNM 63

Query: 500 ITKTIE 517
            T +IE
Sbjct: 64  STYSIE 69


>SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 430

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = +2

Query: 533 PIETQHQKTFIGKIPIMLKIYILL 604
           P ET+HQ  F+ +  I +KIY LL
Sbjct: 24  PNETKHQWIFVPRDLIKIKIYSLL 47


>SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 313

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 17/73 (23%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
 Frame = +2

Query: 233 LVRQQLDSFDEFIQMSVQRIVEDSPP-IELQAEAQHFSGEMETPPKYRLKFDQIYLSKPT 409
           LV      F+E   MS  RI+  SPP +E+     + + ++ +  + +     + ++ P+
Sbjct: 62  LVNTLSKGFNETSNMSYNRILPSSPPTLEIGEIDYNEALQIRSADENQQSVPTVSIASPS 121

Query: 410 HWEKDGAPSPMMP 448
             E   + SP++P
Sbjct: 122 TPELPPSSSPLLP 134


>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 238

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
 Frame = +2

Query: 497 DITKTIEKEHEDPIETQHQ-KTFIGKIPIMLKIYILLTKQFD 619
           + T+ +EKEHE+   T+ +  +F  K+  MLK    L+++ D
Sbjct: 72  NFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEELD 113


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,451,338
Number of Sequences: 5004
Number of extensions: 49347
Number of successful extensions: 128
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)