Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc28l12
(626 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BT003265-1|AAO25022.1| 1176|Drosophila melanogaster LD22387p pro... 271 6e-73
AE014297-1804|AAF55024.1| 1176|Drosophila melanogaster CG3180-PA... 271 6e-73
X05709-1|CAA29180.2| 1123|Drosophila melanogaster RNA polymerase... 195 4e-50
M62972-1|AAA28476.1| 69|Drosophila melanogaster RNA polymerase... 105 4e-23
X58826-1|CAA41631.1| 1135|Drosophila melanogaster RNA polymerase... 64 2e-10
AY121645-1|AAM51972.1| 1146|Drosophila melanogaster LD14594p pro... 62 5e-10
AE013599-1387|AAF58590.1| 1137|Drosophila melanogaster CG8344-PA... 62 5e-10
AE013599-1761|AAF58340.2| 1093|Drosophila melanogaster CG6280-PA... 31 0.97
>BT003265-1|AAO25022.1| 1176|Drosophila melanogaster LD22387p
protein.
Length = 1176
Score = 271 bits (664), Expect = 6e-73
Identities = 128/152 (84%), Positives = 138/152 (90%), Gaps = 1/152 (0%)
Frame = +2
Query: 167 ITPELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSG 346
I+ ELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSP IELQAEAQH SG
Sbjct: 18 ISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPAIELQAEAQHTSG 77
Query: 347 EMETPPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEH 526
E+ETPP++ LKF+QIYLSKPTHWEKDG+PSPMMPNEARLRNLTYSAPLYVDITKT E
Sbjct: 78 EVETPPRFSLKFEQIYLSKPTHWEKDGSPSPMMPNEARLRNLTYSAPLYVDITKTKNVEG 137
Query: 527 EDPIETQHQKTFIGKIPIMLK-IYILLTKQFD 619
DP+ETQHQKTFIGKIPIML+ Y LL++ D
Sbjct: 138 LDPVETQHQKTFIGKIPIMLRSTYCLLSQLTD 169
Score = 31.9 bits (69), Expect = 0.73
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 552 RKHLLARFLLCLRSTYCLLNSLTDR 626
+K + + + LRSTYCLL+ LTDR
Sbjct: 146 QKTFIGKIPIMLRSTYCLLSQLTDR 170
>AE014297-1804|AAF55024.1| 1176|Drosophila melanogaster CG3180-PA
protein.
Length = 1176
Score = 271 bits (664), Expect = 6e-73
Identities = 128/152 (84%), Positives = 138/152 (90%), Gaps = 1/152 (0%)
Frame = +2
Query: 167 ITPELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSG 346
I+ ELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSP IELQAEAQH SG
Sbjct: 18 ISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPAIELQAEAQHTSG 77
Query: 347 EMETPPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEH 526
E+ETPP++ LKF+QIYLSKPTHWEKDG+PSPMMPNEARLRNLTYSAPLYVDITKT E
Sbjct: 78 EVETPPRFSLKFEQIYLSKPTHWEKDGSPSPMMPNEARLRNLTYSAPLYVDITKTKNVEG 137
Query: 527 EDPIETQHQKTFIGKIPIMLK-IYILLTKQFD 619
DP+ETQHQKTFIGKIPIML+ Y LL++ D
Sbjct: 138 LDPVETQHQKTFIGKIPIMLRSTYCLLSQLTD 169
Score = 31.9 bits (69), Expect = 0.73
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 552 RKHLLARFLLCLRSTYCLLNSLTDR 626
+K + + + LRSTYCLL+ LTDR
Sbjct: 146 QKTFIGKIPIMLRSTYCLLSQLTDR 170
>X05709-1|CAA29180.2| 1123|Drosophila melanogaster RNA polymerase
protein.
Length = 1123
Score = 195 bits (476), Expect = 4e-50
Identities = 93/116 (80%), Positives = 102/116 (87%), Gaps = 1/116 (0%)
Frame = +2
Query: 275 MSVQRIVEDSPPIELQAEAQHFSGEMETPPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNE 454
MSVQRIVEDSP IELQAE QH SGE+ETPP++ LKF+QIYLSKPTHWEKDG+PSPMMPNE
Sbjct: 1 MSVQRIVEDSPAIELQAERQHTSGEVETPPRFSLKFEQIYLSKPTHWEKDGSPSPMMPNE 60
Query: 455 ARLRNLTYSAPLYVDITKTIEKEHEDPIETQHQKTFIGKIPIMLK-IYILLTKQFD 619
ARLRNLTYSAPLYVDITKT E DP+ETQHQKTFIGKIPIML+ Y LL++ D
Sbjct: 61 ARLRNLTYSAPLYVDITKTKNVEGLDPVETQHQKTFIGKIPIMLRSTYCLLSQLTD 116
Score = 31.9 bits (69), Expect = 0.73
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 552 RKHLLARFLLCLRSTYCLLNSLTDR 626
+K + + + LRSTYCLL+ LTDR
Sbjct: 93 QKTFIGKIPIMLRSTYCLLSQLTDR 117
>M62972-1|AAA28476.1| 69|Drosophila melanogaster RNA polymerase II
protein.
Length = 69
Score = 105 bits (253), Expect = 4e-23
Identities = 49/52 (94%), Positives = 50/52 (96%)
Frame = +2
Query: 167 ITPELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQ 322
I+ ELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSP IELQ
Sbjct: 18 ISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPAIELQ 69
>X58826-1|CAA41631.1| 1135|Drosophila melanogaster RNA polymerase
III second-largestsubunit protein.
Length = 1135
Score = 63.7 bits (148), Expect = 2e-10
Identities = 41/137 (29%), Positives = 69/137 (50%)
Frame = +2
Query: 197 WIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSGEMETPPKYRL 376
W ++ A+ KGLV+Q +DSF+ FI + +++IV+ + + A+ P + L
Sbjct: 35 WKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIVKANELVTSGAD-----------PLFYL 83
Query: 377 KFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEHEDPIETQHQK 556
K+ + + KP + P+E RLR+ TYSAP+ VDI T + +
Sbjct: 84 KYLDVRVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDIEYTRGTQ-----RIKRNN 138
Query: 557 TFIGKIPIMLKIYILLT 607
IG++P+ML+ LT
Sbjct: 139 LLIGRMPLMLRSNCALT 155
>AY121645-1|AAM51972.1| 1146|Drosophila melanogaster LD14594p
protein.
Length = 1146
Score = 62.5 bits (145), Expect = 5e-10
Identities = 39/131 (29%), Positives = 67/131 (51%)
Frame = +2
Query: 197 WIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSGEMETPPKYRL 376
W ++ A+ KGLV+Q +DSF+ FI + +++IV+ + + A+ P + L
Sbjct: 44 WKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIVKANELVTSGAD-----------PLFYL 92
Query: 377 KFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEHEDPIETQHQK 556
K+ + + KP + P+E RLR+ TYSAP+ VDI T + +
Sbjct: 93 KYLDVRVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDIEYTRGTQ-----RIKRNN 147
Query: 557 TFIGKIPIMLK 589
IG++P+ML+
Sbjct: 148 LLIGRMPLMLR 158
>AE013599-1387|AAF58590.1| 1137|Drosophila melanogaster CG8344-PA
protein.
Length = 1137
Score = 62.5 bits (145), Expect = 5e-10
Identities = 39/131 (29%), Positives = 67/131 (51%)
Frame = +2
Query: 197 WIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPPIELQAEAQHFSGEMETPPKYRL 376
W ++ A+ KGLV+Q +DSF+ FI + +++IV+ + + A+ P + L
Sbjct: 35 WKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIVKANELVTSGAD-----------PLFYL 83
Query: 377 KFDQIYLSKPTHWEKDGAPSPMMPNEARLRNLTYSAPLYVDITKTIEKEHEDPIETQHQK 556
K+ + + KP + P+E RLR+ TYSAP+ VDI T + +
Sbjct: 84 KYLDVRVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDIEYTRGTQ-----RIKRNN 138
Query: 557 TFIGKIPIMLK 589
IG++P+ML+
Sbjct: 139 LLIGRMPLMLR 149
>AE013599-1761|AAF58340.2| 1093|Drosophila melanogaster CG6280-PA
protein.
Length = 1093
Score = 31.5 bits (68), Expect = 0.97
Identities = 24/102 (23%), Positives = 39/102 (38%), Gaps = 6/102 (5%)
Frame = +2
Query: 281 VQRIVEDSPPIELQAEAQHFSGEMETPPKYRLKFDQIYLSKPTHWEKDGAPSPMMPNEAR 460
VQ + D PPI + S + P + + G P E
Sbjct: 25 VQPALSDEPPILTSSSTPPSSAAVSAPGDLPQNLTVEAAATEALSDSSGKELNKQPAEQL 84
Query: 461 LR------NLTYSAPLYVDITKTIEKEHEDPIETQHQKTFIG 568
+R NL + D+ + +++E EDPIET+ + F+G
Sbjct: 85 IREVIEGPNLADEVRVDPDLERELKREGEDPIETETETEFVG 126
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,241,129
Number of Sequences: 53049
Number of extensions: 501203
Number of successful extensions: 1192
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1189
length of database: 24,988,368
effective HSP length: 82
effective length of database: 20,638,350
effective search space used: 2600432100
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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