Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc28h07
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 81 2e-16
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 79 6e-16
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 77 2e-15
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 49 6e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 46 4e-06
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 3e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 5e-04
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 37 0.002
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 32 0.091
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.48
SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce... 25 7.9
SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 25 7.9
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 80.6 bits (190), Expect = 2e-16
Identities = 52/163 (31%), Positives = 74/163 (45%), Gaps = 5/163 (3%)
Frame = +3
Query: 225 SSDVIELTPSN-FDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGA 395
+S V+EL N + + S + +IEF+A WCGHCKSL P Y++ + V +G
Sbjct: 19 ASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGK 78
Query: 396 LDADEHRSVSQKYGVTGFPTIKIF--TGSKHTPYQGQRTAEGFVXXXXXXXXXXXXXNLX 569
+DAD H V+ KY +TGFPT+ F GS+ Y R +
Sbjct: 79 IDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLT---------QFVSEKT 129
Query: 570 XXXXXXXXXXXXVITLTDSNFKELVLXSDDLWLVEFYAPWXGH 698
V+ L NF ++V+ LVEFYA W G+
Sbjct: 130 GIKKRKIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGY 172
Score = 73.7 bits (173), Expect = 2e-14
Identities = 37/103 (35%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +3
Query: 228 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALD 401
S+V+EL NFDK+V + + ++EF+A WCG+CK L P Y+ + K V++ ++
Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKIN 199
Query: 402 ADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAEGFV 521
AD + + + V FPTIK F K P Y+G R+ E +
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLI 242
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 79.0 bits (186), Expect = 6e-16
Identities = 44/101 (43%), Positives = 60/101 (59%), Gaps = 8/101 (7%)
Frame = +3
Query: 228 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--D 401
S+ IEL NF K V ++ F+APWCG+CK LVP Y+K A L ++ V A+ D
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVV-FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCD 89
Query: 402 ADEHRSVSQKYGVTGFPTIK-IFTGSK-----HTPYQGQRT 506
AD++R+V +Y V GFPTIK ++ SK T Y G R+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRS 130
Score = 26.6 bits (56), Expect = 3.4
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +3
Query: 609 ITLTDSNFKELVLXSDDLWLVEFYAPWXGH 698
I L NF++ V LV FYAPW G+
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGY 62
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 77.4 bits (182), Expect = 2e-15
Identities = 34/102 (33%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
Frame = +3
Query: 222 SSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGAL 398
+S++V ++ ++L+T +D++ +++F+APWCGHCK+L PEY+ AA L K + + +
Sbjct: 20 ASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEV 78
Query: 399 DADEHRSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAEGFV 521
D E + +Y + G+PT+ +F G + + Y G R + V
Sbjct: 79 DCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALV 120
Score = 74.9 bits (176), Expect = 1e-14
Identities = 41/103 (39%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +3
Query: 219 DSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVG 392
+S D++ L NFD +V + + ++EF+APWCGHCK+L P Y+K A V V
Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVA 411
Query: 393 ALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAE 512
+DA E+ +S ++GFPTI F K P Y+G RT E
Sbjct: 412 KIDATEN-DIS--VSISGFPTIMFFKANDKVNPVRYEGDRTLE 451
Score = 35.5 bits (78), Expect = 0.007
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +3
Query: 606 VITLTDSNFKELVLXSDDLWLVEFYAPWXGH 698
++ L NF ++V+ LVEFYAPW GH
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGH 387
Score = 29.1 bits (62), Expect = 0.64
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +3
Query: 642 VLXSDDLWLVEFYAPWXGH 698
++ +D + +V+FYAPW GH
Sbjct: 35 LITADKVLMVKFYAPWCGH 53
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.2 bits (112), Expect = 6e-07
Identities = 21/64 (32%), Positives = 40/64 (62%)
Frame = +3
Query: 276 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 455
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 456 IKIF 467
+ +F
Sbjct: 92 MVLF 95
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 46.4 bits (105), Expect = 4e-06
Identities = 22/94 (23%), Positives = 49/94 (52%), Gaps = 1/94 (1%)
Frame = +3
Query: 234 VIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEH 413
V +++ S+ K + D++ +++FFA WCG CK++ P++++ + + +D D+
Sbjct: 2 VKQVSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQL 60
Query: 414 RSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAE 512
++ + GV P+ ++ G K G A+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAK 94
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.9 bits (89), Expect = 3e-04
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +3
Query: 297 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 473
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 474 SK 479
K
Sbjct: 84 GK 85
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 39.5 bits (88), Expect = 5e-04
Identities = 20/83 (24%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +3
Query: 237 IELT-PSNFDKLVTNSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADE 410
+E+T F +++ N E I ++ F+APW CK + + + A+ K V + ++A++
Sbjct: 3 VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEK 61
Query: 411 HRSVSQKYGVTGFPTIKIFTGSK 479
+++ + V P + G+K
Sbjct: 62 FSDIAESFDVNAVPLFVLIHGAK 84
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 37.1 bits (82), Expect = 0.002
Identities = 18/82 (21%), Positives = 36/82 (43%)
Frame = +3
Query: 252 SNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQK 431
++ D +T+ E W I+F++ C C + + A ++G + V ++ + ++
Sbjct: 289 ADIDAALTDK-EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQ 347
Query: 432 YGVTGFPTIKIFTGSKHTPYQG 497
Y + FPT F Y G
Sbjct: 348 YSIQYFPTFLFFKEEAFVEYVG 369
Score = 30.3 bits (65), Expect = 0.28
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 3/80 (3%)
Frame = +3
Query: 237 IELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DAD 407
+ LT ++ + V+ W I+++ P CG CK L P + K V+ + D
Sbjct: 29 VPLTDNDLESEVSKGT--WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 408 EHRSVSQKYGVTGFPTIKIF 467
+ +S + PT+ ++
Sbjct: 87 CSKELSSCANIRAVPTLYLY 106
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 31.9 bits (69), Expect = 0.091
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +3
Query: 198 TGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFF 308
TGS + SDV+ L+ S L+ SD WII FF
Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 29.5 bits (63), Expect = 0.48
Identities = 13/60 (21%), Positives = 24/60 (40%)
Frame = +3
Query: 318 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 497
C C + R +K+ ++ DE + + + + FPT ++F G Y G
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
>SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 459
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/54 (25%), Positives = 21/54 (38%)
Frame = -1
Query: 415 LCSSASKAPTFTMPFRALAAFLYSGTRLLQCPHHGAKNSMIQISSEFVTNLSKL 254
LC K T + + F +CPH G KN+ +Q + SK+
Sbjct: 37 LCMECGKNGTTKLLLTVIPYFREVVLMSFECPHCGFKNAQVQHAETIQPEGSKI 90
>SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 138
Score = 25.4 bits (53), Expect = 7.9
Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
Frame = +3
Query: 324 HCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGV-TGFPTIKIFTG 473
HC KAAR L I++ ++ E S+SQK + PT+ I G
Sbjct: 69 HCTYSQVRGPKAARVLSEILRNRITESKEKLSLSQKEKLFQNLPTVYILHG 119
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,835,562
Number of Sequences: 5004
Number of extensions: 56541
Number of successful extensions: 169
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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