Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc28g04
(610 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|... 171 7e-44
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr... 150 1e-37
SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 29 0.40
SPAC1006.02 |||WD repeat protein, human GNB1L family|Schizosacch... 28 1.2
SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |... 27 1.6
SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 27 1.6
SPCC576.14 |||diphthine synthase |Schizosaccharomyces pombe|chr ... 27 2.1
SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.8
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 27 2.8
SPBPB2B2.16c |||MFS family membrane transporter |Schizosaccharom... 27 2.8
SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.8
SPAC29A4.09 |||rRNA processing protein Rrp17|Schizosaccharomyces... 27 2.8
SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe... 27 2.8
SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting prot... 26 3.7
SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 26 3.7
SPAC10F6.15 |||S. pombe specific UPF0300 family protein 1|Schizo... 26 3.7
SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 3.7
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac... 26 5.0
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 26 5.0
SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14 |Schizo... 25 6.5
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 25 8.7
SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha sub... 25 8.7
>SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|chr
3|||Manual
Length = 542
Score = 171 bits (416), Expect = 7e-44
Identities = 93/177 (52%), Positives = 119/177 (67%), Gaps = 4/177 (2%)
Frame = +1
Query: 91 KHRYKNVG-LSADELRRRREEEGVQLRKQKREQQLFKRRN---VNLPGTNAAGAASQEDI 258
+ YK G ADELRRRRE + +++RKQKRE+ L KRRN V P Q+
Sbjct: 13 RQNYKGKGTFQADELRRRRETQQIEIRKQKREENLNKRRNLVDVQEPAEETI-PLEQDKE 71
Query: 259 NISPSDCITPDMVLAVYLDNPEEQVIATQKFRKLLSKEPNPPIDAVIQSGIVPKFIEFLT 438
N + PD++ A+Y D+ E Q+ AT KFRK LSKE NPPI VI +G+VP+F+EFL+
Sbjct: 72 NDLELELQLPDLLKALYSDDIEAQIQATAKFRKALSKETNPPIQKVIDAGVVPRFVEFLS 131
Query: 439 NHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGSTENVCEQAVWAL 609
H+N L+FEA+WALTN+ASGSS QT +VV+ AV V V LLS S ++V EQAVWAL
Sbjct: 132 -HENNLLKFEASWALTNVASGSSNQTHVVVEANAVPVFVSLLSSSEQDVREQAVWAL 187
Score = 53.6 bits (123), Expect = 2e-08
Identities = 27/83 (32%), Positives = 48/83 (57%)
Frame = +1
Query: 361 LSKEPNPPIDAVIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGA 540
LS N I A+I +GI + +E L H + +Q A ++ NI +G QT+++++ GA
Sbjct: 275 LSDGANEKIQAIIDAGIPRRLVELLM-HPSAQVQTPALRSVGNIVTGDDVQTQVIINCGA 333
Query: 541 VNVLVGLLSGSTENVCEQAVWAL 609
++ L+ LLS + V ++A W +
Sbjct: 334 LSALLSLLSSPRDGVRKEACWTI 356
Score = 32.7 bits (71), Expect = 0.043
Identities = 18/73 (24%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = +1
Query: 394 VIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGS 573
V+++ VP F+ L++ + + +A WAL NIA S V+ G + L+ ++ +
Sbjct: 159 VVEANAVPVFVSLLSSSEQDVRE-QAVWALGNIAGDSPMCRDHVLQCGVLEPLLNIIESN 217
Query: 574 TE-NVCEQAVWAL 609
++ + W L
Sbjct: 218 RRLSMLRNSTWTL 230
Score = 28.7 bits (61), Expect = 0.70
Identities = 16/66 (24%), Positives = 34/66 (51%)
Frame = +1
Query: 367 KEPNPPIDAVIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVN 546
K P P +++ Q ++P + + L +A WA++ ++ G++ + + ++D G
Sbjct: 237 KNPQPDWNSISQ--VIPVLSKLIYTLDEDVL-VDALWAISYLSDGANEKIQAIIDAGIPR 293
Query: 547 VLVGLL 564
LV LL
Sbjct: 294 RLVELL 299
>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
2|||Manual
Length = 539
Score = 150 bits (364), Expect = 1e-37
Identities = 87/178 (48%), Positives = 115/178 (64%), Gaps = 5/178 (2%)
Frame = +1
Query: 91 KHRYKNVGL-SADELRRRREEEGVQLRKQKREQQLFKRRNVNLPGTNAAGA---ASQEDI 258
+ R+K+ G+ ADELRR+REE+ +++RKQKRE+ L KRRN+N N A Q +
Sbjct: 10 RSRFKSKGVFKADELRRQREEQQIEIRKQKREESLNKRRNLNAVLQNDIDVEEEADQSQV 69
Query: 259 NISPS-DCITPDMVLAVYLDNPEEQVIATQKFRKLLSKEPNPPIDAVIQSGIVPKFIEFL 435
+ P + V D+ E Q+ A KFRK LSKE +PPID VI G+V +F++FL
Sbjct: 70 QMEQQMKDEFPKLTADVMSDDIELQLGAVTKFRKYLSKETHPPIDQVIACGVVDRFVQFL 129
Query: 436 TNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGSTENVCEQAVWAL 609
+ + LQFEAAWALTNIASG++ QTRIVVD GAV + LLS ++V EQ VWAL
Sbjct: 130 ESEHH-LLQFEAAWALTNIASGTTDQTRIVVDSGAVPRFIQLLSSPEKDVREQVVWAL 186
Score = 61.7 bits (143), Expect = 8e-11
Identities = 33/106 (31%), Positives = 59/106 (55%), Gaps = 1/106 (0%)
Frame = +1
Query: 295 VLAVYLDNPEEQVIATQKFR-KLLSKEPNPPIDAVIQSGIVPKFIEFLTNHKNPTLQFEA 471
+LA L + + ++I + LS PN I A++ G P+ +E L++ + +Q A
Sbjct: 252 ILAKLLYSEDVEIIVDACWAISYLSDGPNEKIGAILDVGCAPRLVELLSS-PSVNIQTPA 310
Query: 472 AWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGSTENVCEQAVWAL 609
++ NI +G+ QT+I++D GA+N LLS EN+ ++A W +
Sbjct: 311 LRSVGNIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEACWTI 356
Score = 44.0 bits (99), Expect = 2e-05
Identities = 25/74 (33%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
Frame = +1
Query: 394 VIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGS 573
V+ SG VP+FI+ L++ + + + WAL NIA SS V+ G + L+ +L S
Sbjct: 158 VVDSGAVPRFIQLLSSPEKDVRE-QVVWALGNIAGDSSACRDYVLGNGVLQPLLNILQSS 216
Query: 574 TENV--CEQAVWAL 609
+V A W L
Sbjct: 217 ASDVSMLRNATWTL 230
Score = 42.3 bits (95), Expect = 5e-05
Identities = 20/72 (27%), Positives = 40/72 (55%)
Frame = +1
Query: 394 VIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGS 573
+I G + F L+ H+ ++ EA W ++NI +G++ Q + +++ + LV LLS +
Sbjct: 328 IIDCGALNAFPSLLS-HQKENIRKEACWTISNITAGNTQQIQAIIESNLIPPLVHLLSYA 386
Query: 574 TENVCEQAVWAL 609
++A WA+
Sbjct: 387 DYKTKKEACWAI 398
>SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 745
Score = 29.5 bits (63), Expect = 0.40
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +1
Query: 385 IDAVIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGS 504
+DA S I+P+ + ++ +H NP++ A AL + AS +
Sbjct: 359 LDAQNSSQILPELLYYINSHPNPSVASTAVKALGDFASAN 398
>SPAC1006.02 |||WD repeat protein, human GNB1L
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 368
Score = 27.9 bits (59), Expect = 1.2
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +1
Query: 400 QSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDY 534
+SG V ++I L N K TL F+A W + A S Q + V+Y
Sbjct: 213 ESGHVVQYICSLENVKTVTLDFKAVWKMV-YAYKSHSQPVLSVEY 256
>SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 652
Score = 27.5 bits (58), Expect = 1.6
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
Frame = +1
Query: 184 QQLFKRRNVNLPGTNAAGAASQEDINISPSDC-ITPDMVLAVYLDNPEEQVIA 339
++ F+R+NV+L T+ G ++ + SDC I + ++L+ P EQVIA
Sbjct: 527 EEAFERKNVHLKSTDIRGFHAKVPQQSNFSDCGIYALHFVELFLETP-EQVIA 578
>SPBC947.07 |||ribosome biogenesis protein
Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
Length = 233
Score = 27.5 bits (58), Expect = 1.6
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +1
Query: 91 KHRYKNVGLSADELRRRREEEGVQLRKQKREQQLFKRR 204
K + K A + R+ E++ + +R+Q+RE+ L KRR
Sbjct: 176 KEKEKKKSSDAWKERKDNEKKAMLMRQQRREENLKKRR 213
>SPCC576.14 |||diphthine synthase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 283
Score = 27.1 bits (57), Expect = 2.1
Identities = 20/88 (22%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Frame = +1
Query: 157 VQLRKQKREQQLFKRRNVNLPGTNAAGAASQEDINISPS---DCITPD-MVLAVYLDNPE 324
+++++Q E R+ P +A A+Q+ + + + TPD + +AV +
Sbjct: 165 IKVKEQSWENLARGRKVYEPPRYMSASLAAQQMLEVEEDRQENICTPDSLCVAVGRMGSD 224
Query: 325 EQVIATQKFRKLLSKEPNPPIDAVIQSG 408
+QVI ++L + PP+ +V+ G
Sbjct: 225 DQVIFAGTLQELAEHDIGPPLHSVVLVG 252
>SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 485
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/59 (23%), Positives = 25/59 (42%)
Frame = -2
Query: 591 LAHVLRRATQ*SYQHIYCAIIHYDTSLS*RTRGDVCQRPCRLKL*GWILMVCQEFNKFW 415
LA + + + Y IY ++ ++ G + Q + WIL++ FN FW
Sbjct: 156 LADLFEKKDRGKYMVIYFTVLSIGPGIAPIISGFISQSSIGWQWEFWILLILSGFNLFW 214
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 26.6 bits (56), Expect = 2.8
Identities = 18/55 (32%), Positives = 22/55 (40%)
Frame = +1
Query: 370 EPNPPIDAVIQSGIVPKFIEFLTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDY 534
EPN V S + F+E L H N FE A L S S R+ D+
Sbjct: 8 EPNHSCKVVYASRVAETFVEQLKQHVN---LFEFAPKLVGFLSNSDPAARMYADW 59
>SPBPB2B2.16c |||MFS family membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 485
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/59 (23%), Positives = 25/59 (42%)
Frame = -2
Query: 591 LAHVLRRATQ*SYQHIYCAIIHYDTSLS*RTRGDVCQRPCRLKL*GWILMVCQEFNKFW 415
LA + + + Y IY ++ ++ G + Q + WIL++ FN FW
Sbjct: 156 LADLFEKKDRGKYMVIYFTVLSIGPGIAPIISGFISQSSIGWQWEFWILLILSGFNLFW 214
>SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 485
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/59 (23%), Positives = 25/59 (42%)
Frame = -2
Query: 591 LAHVLRRATQ*SYQHIYCAIIHYDTSLS*RTRGDVCQRPCRLKL*GWILMVCQEFNKFW 415
LA + + + Y IY ++ ++ G + Q + WIL++ FN FW
Sbjct: 156 LADLFEKKDRGKYMVIYFTVLSIGPGIAPIISGFISQSSIGWQWEFWILLILSGFNLFW 214
>SPAC29A4.09 |||rRNA processing protein Rrp17|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/38 (31%), Positives = 23/38 (60%)
Frame = +1
Query: 76 MSGGHKHRYKNVGLSADELRRRREEEGVQLRKQKREQQ 189
++G HK + + + +L +++ EE + LRK REQ+
Sbjct: 38 LTGFHKRKVERRKHAQVQLEQQKREERLALRKSLREQR 75
Score = 25.8 bits (54), Expect = 5.0
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = +1
Query: 139 RREEEGVQLRKQKREQQLFKRRNV 210
RR+ VQL +QKRE++L R+++
Sbjct: 48 RRKHAQVQLEQQKREERLALRKSL 71
>SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 886
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/57 (21%), Positives = 28/57 (49%)
Frame = +1
Query: 226 NAAGAASQEDINISPSDCITPDMVLAVYLDNPEEQVIATQKFRKLLSKEPNPPIDAV 396
N +G ++ +I + + V +DNP+ + +T K+R + P+ P+ ++
Sbjct: 729 NVSGLPAETEIAYHHTLSASNSYVFTDLVDNPDSMLKSTLKYRFVFKFSPSQPLSSL 785
>SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting protein
1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 26.2 bits (55), Expect = 3.7
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 18 TNKNLKYFFFSLRCSVFKYNVWRSQTSLQE 107
T +LKY++ S CS F YN + S S E
Sbjct: 143 TTIDLKYWYCS--CSQFSYNAFNSSRSFDE 170
>SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 658
Score = 26.2 bits (55), Expect = 3.7
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +1
Query: 433 LTNHKNPTLQFEAAWALTNIASGSSGQTRIVVDYGAVNVLVGLLSGSTEN 582
L ++N + + LTN A GSSG + AV+ L+G GS+ N
Sbjct: 473 LFQNRNLLTKLAQSGVLTNAAQGSSGG----LSAAAVSGLIGSFMGSSSN 518
>SPAC10F6.15 |||S. pombe specific UPF0300 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 432
Score = 26.2 bits (55), Expect = 3.7
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 211 NLPGTNAAGAASQEDINISPSDCITPDMVLAVYLDNPEEQ 330
NLP + AG+A + S +C+T + L LD P ++
Sbjct: 380 NLPNFSEAGSAKSSNFCSSKDNCLTNRLTLNC-LDTPSDE 418
>SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 364
Score = 26.2 bits (55), Expect = 3.7
Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = +1
Query: 91 KHRYKNVGLSADELR--RRREEEGVQLRKQKREQQL 192
K R ++ + D L+ +RR EG + RK +REQ L
Sbjct: 248 KSRIVHIDEAIDRLKEVKRRRIEGFERRKMRREQNL 283
>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
Hip3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1630
Score = 25.8 bits (54), Expect = 5.0
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = +1
Query: 445 KNPTLQFEAAWALTNIASGSSGQTRIVVD--YGAV-NVLVGLLSGSTENV 585
+N + FEAA AL SGS Q ++++ Y + N+ + GS E V
Sbjct: 1247 ENALVHFEAAAALAPTKSGSGSQQALLIEPHYALLSNLSKAAIEGSVEIV 1296
>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 578
Score = 25.8 bits (54), Expect = 5.0
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +1
Query: 118 SADELRRRREEEGVQLRKQKREQQLFKRRNVNLPGTN 228
S +EL+R +EE RK+K+ + K+ + P +
Sbjct: 54 SVEELKRSADEEASVKRKEKKSKHEHKKHKKDKPSAD 90
>SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 566
Score = 25.4 bits (53), Expect = 6.5
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 259 NISPSDCITPDMVLAVYLDNPEEQVIATQKFRKLLSKEPN 378
N++ S+ + D++ Y D P + RKLL K+ N
Sbjct: 428 NMTFSNILHKDVIFPEYADAPSISSLCKNLIRKLLVKDEN 467
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 3/49 (6%)
Frame = +1
Query: 85 GHKHRYKNVGLSADELRRR---REEEGVQLRKQKREQQLFKRRNVNLPG 222
GH + + L + + R+ R +QL ++ +F RR+V +PG
Sbjct: 525 GHSTPHNSPSLLSVKKRQNNHIRSNTLIQLNSNSKDSTIFPRRSVTVPG 573
>SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha
subunit Tfg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 490
Score = 25.0 bits (52), Expect = 8.7
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +1
Query: 145 EEEGVQLRK--QKREQQLFKRRNVNLPGTNAAGAASQEDINISP----SDCITPDMVLAV 306
EEE +Q + QKRE++L R + N TN + ++SP S +P+ + +
Sbjct: 360 EEEVLQEEEELQKREEKLKSRFSANASKTNTPRPLERTPSSVSPVKASSQLQSPNTSIQI 419
Query: 307 YLDNPEEQVI 336
P+EQ+I
Sbjct: 420 ---RPQEQLI 426
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,209,055
Number of Sequences: 5004
Number of extensions: 40511
Number of successful extensions: 192
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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