Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc28f22
(668 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subuni... 200 2e-52
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 44 3e-05
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 33 0.037
SPCC737.07c |||DNA polymerase alpha-associated DNA helicase A |S... 29 0.46
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 29 0.46
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 29 0.61
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 28 1.1
SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 27 1.8
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 27 3.2
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 27 3.2
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 3.2
SPAC27E2.10c |rfc3|SPAPJ698.01c|DNA replication factor C complex... 27 3.2
SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch... 26 4.3
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 26 5.6
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 25 7.5
SPBC244.02c |||U3 snoRNP-associated protein Utp6 |Schizosaccharo... 25 9.9
SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |S... 25 9.9
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 9.9
SPBC1921.07c ||SPBC21D10.13|SAGA complex subunit Sgf29 |Schizosa... 25 9.9
>SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subunit
Rad15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 772
Score = 200 bits (487), Expect = 2e-52
Identities = 95/184 (51%), Positives = 123/184 (66%)
Frame = +1
Query: 115 MKLTVDGLLVYFPYDYIYPEQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYM 294
MK +D L + FPY IYPEQY YM +LK +LDA G LLEMPSGTGKTISLLSLIV+Y
Sbjct: 1 MKFYIDDLPILFPYPRIYPEQYQYMCDLKHSLDAGGIALLEMPSGTGKTISLLSLIVSYQ 60
Query: 295 IQNPHHVRKLIYCSRTVPEIEKVLEELKNLFNYYEKSQGEKPNLTGVVLSSRKNLCIHPD 474
P H RKLIYCSRT+ EI+K L ELK L Y G + G+ L+SRKNLC+HP
Sbjct: 61 QHYPEH-RKLIYCSRTMSEIDKALAELKRLMAYRTSQLGYEEPFLGLGLTSRKNLCLHPS 119
Query: 475 VSREREGKLVDGKCHALTASYIRDRHERDSSVPICQFYEGFNREGKESMLPYGVYTMDDM 654
V RE+ G +VD +C +LTA ++R++ VP C+F++ S++ GV+T+DD+
Sbjct: 120 VRREKNGNVVDARCRSLTAGFVREQRLAGMDVPTCEFHDNLEDLEPHSLISNGVWTLDDI 179
Query: 655 KQYG 666
+YG
Sbjct: 180 TEYG 183
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 43.6 bits (98), Expect = 3e-05
Identities = 26/79 (32%), Positives = 45/79 (56%)
Frame = +1
Query: 298 QNPHHVRKLIYCSRTVPEIEKVLEELKNLFNYYEKSQGEKPNLTGVVLSSRKNLCIHPDV 477
+ P V+K+ + SRT +++++++E+K L N Q + V L+SRKNLCI+ +V
Sbjct: 206 EKPPIVQKIYFTSRTHSQLQQLVQEIKKLNN-----QTFSTPIRVVSLASRKNLCINNEV 260
Query: 478 SREREGKLVDGKCHALTAS 534
+ R ++ KC L S
Sbjct: 261 RKLRPTSALNEKCIELQGS 279
Score = 31.1 bits (67), Expect = 0.15
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +1
Query: 145 YFPYDYIYPEQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYM 294
Y PYD Q +M L ++ G+ E P+GTGK++SL+ + ++
Sbjct: 17 YTPYDI----QLEFMRSLYSSISDGKIGIFESPTGTGKSLSLICASLTWL 62
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 33.1 bits (72), Expect = 0.037
Identities = 16/55 (29%), Positives = 33/55 (60%), Gaps = 1/55 (1%)
Frame = +1
Query: 205 ALDAKGHGLLEMPSGTGKTISLLSLIVAYMIQ-NPHHVRKLIYCSRTVPEIEKVL 366
ALD G L++ P GTGKT +++ +I A ++ + +H+ + S++ +++L
Sbjct: 1141 ALDNNGFTLIQGPPGTGKTKTIIGIISALLVDLSRYHITRPNQQSKSTESKQQIL 1195
>SPCC737.07c |||DNA polymerase alpha-associated DNA helicase A
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 660
Score = 29.5 bits (63), Expect = 0.46
Identities = 14/62 (22%), Positives = 35/62 (56%)
Frame = +1
Query: 196 LKRALDAKGHGLLEMPSGTGKTISLLSLIVAYMIQNPHHVRKLIYCSRTVPEIEKVLEEL 375
+K ++ K L+ P GTGKT +L+ +I +++N ++++ C + ++ +++ L
Sbjct: 218 VKFSIAVKELSLIHGPPGTGKTHTLVEIIQQLVLRN----KRILVCGASNLAVDNIVDRL 273
Query: 376 KN 381
+
Sbjct: 274 SS 275
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 29.5 bits (63), Expect = 0.46
Identities = 29/104 (27%), Positives = 43/104 (41%), Gaps = 6/104 (5%)
Frame = +1
Query: 172 EQYAYMLELKRAL-DAKGHGLLEMPSGTGKTISLLSLIVAYMIQNPHHVRKLIYCSRTV- 345
E Y LE AL D+ +G+L G GKTI ++L+ + + LI +V
Sbjct: 449 EYQQYGLEWLTALHDSNTNGILADEMGLGKTIQTIALLAHLACEKENWGPHLIIVPTSVM 508
Query: 346 ----PEIEKVLEELKNLFNYYEKSQGEKPNLTGVVLSSRKNLCI 465
E +K L K + YY Q K +G ++CI
Sbjct: 509 LNWEMEFKKFLPGFK-ILTYYGNPQERKEKRSGWYKPDTWHVCI 551
>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
heterochromatin assembly Hrr1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1015
Score = 29.1 bits (62), Expect = 0.61
Identities = 21/84 (25%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
Frame = +1
Query: 127 VDGLLVYFPYDYIYPEQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYMIQNP 306
V+ L + P +YI L+ +++ K +++ P GTGK+ L I +++N
Sbjct: 359 VNALEPFGPGEYILDSS---QLKAYQSMLTKRLSIIQGPPGTGKSFVTLKAIET-LLENT 414
Query: 307 H-HVRKLIYCSRTVPEIEKVLEEL 375
H HV ++ +T ++++L L
Sbjct: 415 HSHVLPILVACQTNHAVDQILIRL 438
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = +1
Query: 169 PEQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLI 282
P+ A E RA+ +K L++ P GTGKT++ S++
Sbjct: 391 PKLNASQSEAVRAVLSKPLSLIQGPPGTGKTVTSASVV 428
>SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog
Rhp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 963
Score = 27.5 bits (58), Expect = 1.8
Identities = 27/100 (27%), Positives = 49/100 (49%), Gaps = 5/100 (5%)
Frame = +1
Query: 163 IYPEQYAYMLELKRALDAK-GHGLLEMPSGTGKTISLLSLIVAYMIQNPH-HVRKLIYCS 336
+ P Q + LKR D+ G G+L G GKTI ++L+++ P V ++
Sbjct: 357 LLPFQREGVYWLKRQEDSSFGGGILADEMGMGKTIQTIALLLSEPRGKPTLVVAPVVAIM 416
Query: 337 RTVPEIEKVLEELKNLFNYYEKSQ---GEKPNLTGVVLSS 447
+ EI+ + + + YY +++ GE+ + VVL+S
Sbjct: 417 QWKEEIDTHTNKALSTYLYYGQARDISGEELSSYDVVLTS 456
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 26.6 bits (56), Expect = 3.2
Identities = 18/61 (29%), Positives = 34/61 (55%)
Frame = +1
Query: 196 LKRALDAKGHGLLEMPSGTGKTISLLSLIVAYMIQNPHHVRKLIYCSRTVPEIEKVLEEL 375
LK+ A+ + L+ GTGKT ++ SLI + + + +K++ S T ++ +L +L
Sbjct: 941 LKKCHAAEHYSLILGMPGTGKTTTISSLIRSLLAKK----KKILLTSFTHLAVDNILIKL 996
Query: 376 K 378
K
Sbjct: 997 K 997
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 304 PHHVRKLIYCSRTVPEIEKVLEELKNLFNYYEKSQG 411
P+H K++Y SR + L++ NLF + K G
Sbjct: 9 PNHSCKVVYASRVAETFVEQLKQHVNLFEFAPKLVG 44
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 26.6 bits (56), Expect = 3.2
Identities = 16/55 (29%), Positives = 30/55 (54%)
Frame = +1
Query: 172 EQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYMIQNPHHVRKLIYCS 336
E +A+M ++++ +K G++ G GKTI ++L++ Q+P LI S
Sbjct: 256 EGHAWMESMEQS--SKCGGVMADDMGLGKTIQTIALLLTQKSQDPLRKTNLIVVS 308
>SPAC27E2.10c |rfc3|SPAPJ698.01c|DNA replication factor C complex
subunit Rfc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 342
Score = 26.6 bits (56), Expect = 3.2
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 223 HGLLEMPSGTGKTISLLSLIVAYMIQNPHHVRKLIYCSRTVPE-IEKVLEELKN 381
H L P GTGKT ++L+ A I P++ +L+ + + I+ V E++KN
Sbjct: 58 HMLFYGPPGTGKTSTILA--CARKIYGPNYRNQLMELNASDDRGIDAVREQIKN 109
>SPBC354.01 |gtp1|SPBC649.06|GTP binding protein
Gtp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.3
Identities = 18/58 (31%), Positives = 33/58 (56%), Gaps = 6/58 (10%)
Frame = +1
Query: 355 EKVLEELKNLFNYYEKSQGEKPNLTGVVL----SSRKNLC--IHPDVSREREGKLVDG 510
E++ EEL NL+ Y K +GE P+ + ++ S+ + +C IH ++ + + LV G
Sbjct: 280 ERIWEEL-NLYRIYTKRKGEMPDFSEALIVRKGSTIEQVCNRIHRTLAEQLKYALVWG 336
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 25.8 bits (54), Expect = 5.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 172 EQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYM 294
E AY + D G L++ P GTGKT ++L +I A +
Sbjct: 1273 EPQAYAIYASSVND--GFTLIQGPPGTGKTKTILGMIGAVL 1311
>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 7.5
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +1
Query: 226 GLLEMPSGTGKTISLLSLIVAYMIQNPHH 312
G+L G GKTI +++LI+++ + H
Sbjct: 258 GILADDMGLGKTIQMIALILSHPLPKKKH 286
>SPBC244.02c |||U3 snoRNP-associated protein Utp6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 488
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +1
Query: 328 YCSRTVPEIEKVLEELKNLFN 390
Y ++VPE+E +LE KN+FN
Sbjct: 8 YMEQSVPELEDLLE--KNIFN 26
>SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 345
Score = 25.0 bits (52), Expect = 9.9
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +3
Query: 129 RWFIGLFPIRLH 164
RWF FPIRLH
Sbjct: 92 RWFCHYFPIRLH 103
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 25.0 bits (52), Expect = 9.9
Identities = 17/60 (28%), Positives = 28/60 (46%)
Frame = +1
Query: 118 KLTVDGLLVYFPYDYIYPEQYAYMLELKRALDAKGHGLLEMPSGTGKTISLLSLIVAYMI 297
K D +VY + Y Y L + D +G G+L G GKT+ +L L++ + +
Sbjct: 251 KSLFDDRMVYVNHVYGYMTFSKEKTYLLASGDIRG-GILADEMGMGKTLEVLGLVLHHQL 309
>SPBC1921.07c ||SPBC21D10.13|SAGA complex subunit Sgf29
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = +1
Query: 349 EIEKVLEELKNLFNYYEKSQGEKPNLTGVVLS-SRKN 456
E+E ++ L+ + N EK+ EKP +T L+ SR+N
Sbjct: 66 ELEHTMQSLEMIINVLEKA-NEKPVITNSPLTRSRRN 101
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,645,812
Number of Sequences: 5004
Number of extensions: 53420
Number of successful extensions: 183
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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