BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc27o08
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g28690.1 68417.m04099 expressed protein 33 0.14
At5g27620.1 68418.m03309 cyclin family protein similar to SP|P51... 31 0.73
At3g10060.1 68416.m01206 immunophilin, putative / FKBP-type pept... 29 1.7
At5g13130.1 68418.m01504 hypothetical protein low similarity to ... 29 2.2
At2g24130.1 68415.m02883 leucine-rich repeat transmembrane prote... 29 2.2
At1g63420.1 68414.m07172 expressed protein 29 2.9
At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase fa... 28 5.1
At4g15440.1 68417.m02361 hydroperoxide lyase (HPL1) identical to... 27 6.8
At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1) ide... 27 6.8
At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative st... 27 6.8
At4g29990.1 68417.m04266 light repressible receptor protein kina... 27 9.0
At2g18876.2 68415.m02202 expressed protein 27 9.0
At2g18876.1 68415.m02201 expressed protein 27 9.0
At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase fa... 27 9.0
>At4g28690.1 68417.m04099 expressed protein
Length = 448
Score = 33.1 bits (72), Expect = 0.14
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = +3
Query: 273 DDDQMRYVLQTEPTVHHTLLEKLMKQELRPYRNSGEVYCTDFEHIKRA 416
DDD +L +PT T++E + GEV C DF H + A
Sbjct: 54 DDDDDCVILDFDPTAKETVIETCETDGVLVVGQKGEVACRDFPHPRHA 101
>At5g27620.1 68418.m03309 cyclin family protein similar to SP|P51946
Cyclin H (MO15-associated protein) {Homo sapiens};
contains Pfam profile PF00134: Cyclin, N-terminal domain
Length = 336
Score = 30.7 bits (66), Expect = 0.73
Identities = 24/83 (28%), Positives = 37/83 (44%), Gaps = 2/83 (2%)
Frame = +3
Query: 207 LFKIGQTTNLTRRLVSLNCGRADDDQMRYVLQTEPTVHHTLLE--KLMKQELRPYRNSGE 380
LF GQ + R+ + G D D+ + ++P HT E KL+ +N
Sbjct: 224 LFPPGQLALASLRIANGVLGVIDFDRYLENIVSQPNSEHTTSELTKLLDNIEYLVKNYKC 283
Query: 381 VYCTDFEHIKRALETCLPHCSQN 449
D +HI R L++CL H S +
Sbjct: 284 PSEKDMKHINRKLKSCLGHSSSH 306
>At3g10060.1 68416.m01206 immunophilin, putative / FKBP-type
peptidyl-prolyl cis-trans isomerase, putative
Pfam:PF-254: FKBP-type peptidyl-prolyl cis-trans
isomerases
Length = 230
Score = 29.5 bits (63), Expect = 1.7
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +1
Query: 139 SLIPLPSRAISMRPRRCCTPKGICSRSARLQT 234
S IP PSR +P RC P C + R +T
Sbjct: 17 SSIPFPSRKRQSKPYRCSLPSPGCEKVIRTET 48
>At5g13130.1 68418.m01504 hypothetical protein low similarity to
microrchidia [Mus musculus] GI:5410255
Length = 706
Score = 29.1 bits (62), Expect = 2.2
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +3
Query: 279 DQMRYVLQTEPTVHHTLLEKLMKQELRPYR 368
D R +L+ E HH++L+ +MK E + Y+
Sbjct: 365 DNFRIILRGEDVEHHSVLDDMMKIEEKTYK 394
>At2g24130.1 68415.m02883 leucine-rich repeat transmembrane protein
kinase, putative
Length = 980
Score = 29.1 bits (62), Expect = 2.2
Identities = 21/84 (25%), Positives = 42/84 (50%), Gaps = 3/84 (3%)
Frame = -1
Query: 332 EQRVVHGGLG--LQHVPHLVVVGAAA-IQRNQSPRQVCSLADLEQIPFGVQQRRGRIEMA 162
+Q +HG + + ++ +L ++ ++ + PR++C L+ LE++ G I M
Sbjct: 305 DQNRIHGSIPPEISNLLNLTLLNLSSNLLSGPIPRELCKLSKLERVYLSNNHLTGEIPME 364
Query: 161 LDGSGIKLYCRLGVLEQAQRRLSG 90
L RLG+L+ ++ LSG
Sbjct: 365 LGD-----IPRLGLLDVSRNNLSG 383
>At1g63420.1 68414.m07172 expressed protein
Length = 578
Score = 28.7 bits (61), Expect = 2.9
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +2
Query: 23 LQDDQRGGAAKLVLRTRVAPVHRPTV-GVEPAPRRPSDSK 139
L ++ G ++ L + P HRPT+ ++P P R S+ K
Sbjct: 89 LNQNRSGSCSRTPLLNKKKPSHRPTITTIKPVPVRVSEKK 128
>At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 650
Score = 27.9 bits (59), Expect = 5.1
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = -1
Query: 308 LGLQHVPHLVVVGAAAIQRNQSPRQVCSLADLEQIPFGVQQRRGRIEMALDGSGIKLYCR 129
+G++ +P +G A RN+ V + ++ PF +G + AL G+K R
Sbjct: 165 MGVEPLPVHSGLGGAYYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVR 224
Query: 128 LG 123
+G
Sbjct: 225 VG 226
>At4g15440.1 68417.m02361 hydroperoxide lyase (HPL1) identical to
hydroperoxide lyase GI:3822403 from [Arabidopsis
thaliana]
Length = 384
Score = 27.5 bits (58), Expect = 6.8
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +2
Query: 146 FRSRRGPFLCGHDAAVRRKESVQDRP-DYKLDAATG 250
F ++G LCG+ V R +V D P ++K D G
Sbjct: 283 FEVKKGELLCGYQPLVMRDANVFDEPEEFKPDRYVG 318
>At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1)
identical to 2-isopropylmalate synthase (IMS1)
[Arabidopsis thaliana] GI:12330687; identical to cDNA
2-isopropylmalate synthase (IMS1) GI:12330686
Length = 631
Score = 27.5 bits (58), Expect = 6.8
Identities = 20/76 (26%), Positives = 31/76 (40%)
Frame = -1
Query: 461 LNGLVLRAVGQARLERALDVLEIRAVDLAAVAVRSQLLFHQLFEQRVVHGGLGLQHVPHL 282
+NG+ RA G A LE + ++ R + H + ++V G+Q PH
Sbjct: 320 INGIGERA-GNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHK 378
Query: 281 VVVGAAAIQRNQSPRQ 234
+VGA A Q
Sbjct: 379 AIVGANAFAHESGIHQ 394
>At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative
strong similarity to 2-isopropylmalate synthase (IMS1)
[Arabidopsis thaliana] GI:12330687; contains Pfam
profile PF00682: HMGL-like
Length = 631
Score = 27.5 bits (58), Expect = 6.8
Identities = 20/76 (26%), Positives = 31/76 (40%)
Frame = -1
Query: 461 LNGLVLRAVGQARLERALDVLEIRAVDLAAVAVRSQLLFHQLFEQRVVHGGLGLQHVPHL 282
+NG+ RA G A LE + ++ R + H + ++V G+Q PH
Sbjct: 322 INGIGERA-GNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHK 380
Query: 281 VVVGAAAIQRNQSPRQ 234
+VGA A Q
Sbjct: 381 AIVGANAFAHESGIHQ 396
>At4g29990.1 68417.m04266 light repressible receptor protein kinase
identical to light repressible receptor protein kinase
[Arabidopsis thaliana] gi|1321686|emb|CAA66376
Length = 876
Score = 27.1 bits (57), Expect = 9.0
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = -1
Query: 506 LFTTTRDDSTKARNKLNGLVLRAVGQARLERALDVLEIRAVD 381
LFT TR K+N +VL+ G + L ++ +EI ++
Sbjct: 314 LFTDTRSTVDPVGRKMNEIVLQRTGVSTLPPIINAIEIYQIN 355
>At2g18876.2 68415.m02202 expressed protein
Length = 284
Score = 27.1 bits (57), Expect = 9.0
Identities = 20/64 (31%), Positives = 32/64 (50%)
Frame = -1
Query: 284 LVVVGAAAIQRNQSPRQVCSLADLEQIPFGVQQRRGRIEMALDGSGIKLYCRLGVLEQAQ 105
LV G +QSP + D+ +PF + RG+IE +L + + R+G L AQ
Sbjct: 170 LVANGRHGADPSQSP--LGGKTDVFDLPF--RMARGQIEDSLRSKMVSIKERMGQLVDAQ 225
Query: 104 RRLS 93
+ +S
Sbjct: 226 KEVS 229
>At2g18876.1 68415.m02201 expressed protein
Length = 382
Score = 27.1 bits (57), Expect = 9.0
Identities = 20/64 (31%), Positives = 32/64 (50%)
Frame = -1
Query: 284 LVVVGAAAIQRNQSPRQVCSLADLEQIPFGVQQRRGRIEMALDGSGIKLYCRLGVLEQAQ 105
LV G +QSP + D+ +PF + RG+IE +L + + R+G L AQ
Sbjct: 268 LVANGRHGADPSQSP--LGGKTDVFDLPF--RMARGQIEDSLRSKMVSIKERMGQLVDAQ 323
Query: 104 RRLS 93
+ +S
Sbjct: 324 KEVS 327
>At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 622
Score = 27.1 bits (57), Expect = 9.0
Identities = 15/62 (24%), Positives = 30/62 (48%)
Frame = -1
Query: 308 LGLQHVPHLVVVGAAAIQRNQSPRQVCSLADLEQIPFGVQQRRGRIEMALDGSGIKLYCR 129
+G++ +P +G A R++ + V + ++ PF +G + AL G+K R
Sbjct: 157 MGVEPIPVNGGLGGAYYFRDEKGQSVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVR 216
Query: 128 LG 123
+G
Sbjct: 217 VG 218
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,299,110
Number of Sequences: 28952
Number of extensions: 219168
Number of successful extensions: 598
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 598
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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