BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc27m06
(459 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 28 0.60
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 1.0
SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c... 27 1.8
SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 26 2.4
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 25 4.2
SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces pom... 25 5.6
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 24 9.7
SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70 |Schi... 24 9.7
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 24 9.7
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 24 9.7
SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 24 9.7
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 28.3 bits (60), Expect = 0.60
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -1
Query: 417 SNLNDYFEECFLLHHFRYRIHLYLCLPIG 331
S L DY + HHF + H+Y C IG
Sbjct: 445 SPLADYGNTYYNDHHFHWGYHIYACAVIG 473
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1811
Score = 27.5 bits (58), Expect = 1.0
Identities = 14/55 (25%), Positives = 25/55 (45%)
Frame = +3
Query: 261 SVLKKEITHFFDNDDIXPPASLNSQLGDINIDECDSENDVKENILQSNHSDLIDS 425
S +K ++ D D P S + +IN ++E D++ N+ D +DS
Sbjct: 33 STIKSRVSDEIDEHDSIPEQSNTEKSIEINDKNLEAEKDIESNLSLRPPEDDLDS 87
>SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1316
Score = 26.6 bits (56), Expect = 1.8
Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 4/61 (6%)
Frame = -3
Query: 277 SFFKTDISGNIFLIVGLSRTSCSSISMLSTKSSAIFTLHEVRSIIF----STFVTQGSQM 110
S K D G I ++ R S ++STK S I + IIF +T V QG
Sbjct: 491 SLKKVDFLGQIAMMSATDREEVKSSIVVSTKKSTILEFAKEIEIIFYKKGTTIVRQGDHA 550
Query: 109 E 107
+
Sbjct: 551 D 551
>SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 26.2 bits (55), Expect = 2.4
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +3
Query: 327 NSQLGDINIDECDSENDVKENILQSNHSDLIDS 425
NS+L N+ +CD+ +D ++N + S D+
Sbjct: 89 NSELSQENLTKCDTTHDFEKNCSEKTDSTSADN 121
>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1098
Score = 25.4 bits (53), Expect = 4.2
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
Frame = +3
Query: 204 IEEHDVLDNPTIRKIF---PDISVLKKEITHFFDNDDIXPPASLNSQLGDINIDEC 362
+E I KIF PD++VL +E+ + N+D+ A LN+ + D+C
Sbjct: 278 VERECYYSENAISKIFQNYPDLAVLFEELQYAEGNNDLL-SAILNTNKYTLRPDDC 332
>SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 470
Score = 25.0 bits (52), Expect = 5.6
Identities = 11/39 (28%), Positives = 18/39 (46%)
Frame = -3
Query: 337 NWEFNEAGGXISSLSKKCVISFFKTDISGNIFLIVGLSR 221
NW+ ++ ISS KK + S D G + + S+
Sbjct: 255 NWKPDKQAFNISSFKKKLIPSLLHLDTDGRVLRVDSFSK 293
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 24.2 bits (50), Expect = 9.7
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +3
Query: 285 HFFDNDDIXPPASLNSQLGDINIDECDSENDVKENILQSNHSDLIDS 425
H +N +I PP++ G N + S++ + +L S LIDS
Sbjct: 149 HAPNNSNIQPPSAAPPVPGKENYNAVGSKSPNEPELLNSLDPSLIDS 195
>SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 615
Score = 24.2 bits (50), Expect = 9.7
Identities = 12/55 (21%), Positives = 29/55 (52%)
Frame = +3
Query: 99 LYISI*LPCVTKVLKIILRTSCKVKMAEDFVDSIDIEEHDVLDNPTIRKIFPDIS 263
+YI I CV K+L + + ++K+ E V+ + +E ++ + ++ P ++
Sbjct: 209 IYIKIKGECVVKLLHAVSMRTDEIKLNEGSVNFVTGKEDVSINLVALSRLLPAVA 263
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 24.2 bits (50), Expect = 9.7
Identities = 16/56 (28%), Positives = 27/56 (48%)
Frame = +3
Query: 165 KVKMAEDFVDSIDIEEHDVLDNPTIRKIFPDISVLKKEITHFFDNDDIXPPASLNS 332
K++ ED +++ E+ V D IRK FPD+S + ++ + P L S
Sbjct: 275 KLREPEDDIENRQFSEY-VADR--IRKSFPDVSFKNLHVLSCMEDSVMSPFRELGS 327
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 24.2 bits (50), Expect = 9.7
Identities = 10/41 (24%), Positives = 22/41 (53%)
Frame = -3
Query: 286 CVISFFKTDISGNIFLIVGLSRTSCSSISMLSTKSSAIFTL 164
C++ + ++ G +++G S +S+L K++A TL
Sbjct: 150 CILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTL 190
>SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 507
Score = 24.2 bits (50), Expect = 9.7
Identities = 15/49 (30%), Positives = 27/49 (55%)
Frame = -3
Query: 268 KTDISGNIFLIVGLSRTSCSSISMLSTKSSAIFTLHEVRSIIFSTFVTQ 122
K D+ + I GLS S + ++M + SSA FT E+ +F+ +++
Sbjct: 167 KFDLGNDDQGIWGLSAMSAAEVNMTTGDSSASFT--ELAQAVFNEIMSR 213
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,774,700
Number of Sequences: 5004
Number of extensions: 37061
Number of successful extensions: 127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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